scholarly journals Prevention of Assay Interference in Infectious-Disease Serology Tests Done on the Liaison Platform

2008 ◽  
Vol 15 (5) ◽  
pp. 891-892 ◽  
Author(s):  
Mario Berth ◽  
Eugene Bosmans
Keyword(s):  
Infectious Disease ◽  
Polyvinyl Alcohol ◽  
Epstein Barr Virus ◽  
Immunoglobulin M ◽  
Systematic Evaluation ◽  
Barr Virus ◽  
Epstein Barr ◽  
Magnetic Microparticle ◽  
Positive Results ◽  
Assay Interference

ABSTRACT Immunoassay interference causing unexpected reactive results in magnetic-microparticle-based assays was detected. A systematic evaluation of Liaison Epstein-Barr virus immunoglobulin M showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.

scholarly journals Acute Parvovirus B19 Infection Frequently Causes False-Positive Results in Epstein-Barr Virus- and Herpes Simplex Virus-Specific Immunoglobulin M Determinations Done on the Liaison Platform

2008 ◽  
Vol 16 (3) ◽  
pp. 372-375 ◽  
Author(s):  
Mario Berth ◽  
Eugene Bosmans
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Positive Result ◽  
Epstein Barr Virus ◽  
Parvovirus B19 ◽  
Immunoglobulin M ◽  
Barr Virus ◽  
Epstein Barr ◽  
Simplex Virus ◽  
Assay Interference

ABSTRACT During an outbreak of parvovirus B19 we collected serum samples from 68 nonpregnant patients in the region of Antwerp (Belgium). Fifty-seven (84%) of the parvovirus B19 immunoglobulin M (IgM)-positive sera had a positive result for Epstein-Barr virus (EBV) IgM by Liaison testing, 61 (90%) had a positive result for herpes simplex virus (HSV) IgM, 20 (29%) samples were positive for cytomegalovirus IgM, and 15 (22%) had a positive result for Borrelia burgdorferi sensu lato IgM. As assay interference was suspected, sera were further investigated by using additional infectious-disease serology tests and by performing various interference elimination procedures. We could show that the EBV IgM and HSV IgM results were false positives due to aspecific IgM reactions with the solid phase. All samples were also analyzed by a modified Liaison EBV IgM assay, based on the addition of polyvinylpyrrolidone and polyvinyl alcohol to the dilution buffer, which partially eliminated this type of assay interference. Although the Liaison is a very convenient, automated immunoassay platform, this study demonstrates the potential for improvement of mainly the EBV IgM and HSV IgM tests.

Epstein-Barr Virus Infectious Mononucleosis in Children

PEDIATRICS ◽  
1985 ◽  
Vol 75 (6) ◽  
pp. 1011-1019 ◽  
Author(s):  
Ciro Valent Sumaya ◽  
Yasmin Ench
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Immunoglobulin M ◽  
Antibody Responses ◽  
Adult Patients ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
Age Related ◽  
Slide Test ◽  
Epstein Barr

An investigation was performed to address the need to establish the rate of positive heterophil antibody responses, oropharyngeal isolation of Epstein-Barr virus (EBV), and the evolving pattern of EBV-specific antibody responses among children with documented EBV-infectious mononucleosis. Findings showed that the rate of heterophil antibody responses appeared to increase progressively with advancing age from infancy up to 4 years, after which the rates approached values similar to that reported in young adult patients. The rapid slide test detected a heterophil antibody response as frequently as the Paul-Bunnell-Davidsohn horse cell test, except in children less than 4 years old. The decreased sensitivity found with the rapid slide test in the very young was associated with their less intense heterophil response. The younger group of children also developed a lower acute mean titer and, as a result, a decreased persistence of immunoglobulin M antibody to EBV-capsid antigen, whereas they had more frequent responses to EBV-early antigen directed to restricted component than both the older subjects and adults reported elsewhere. Antibodies to EBV-nuclear antigen, characteristically a late-onset antibody, tended to develop earlier than noted in adult patients. In contrast, the prevalence and continued excretion of EBV from oropharyngeal secretions was similar to that reported in adult patients. It is speculated that these age-related differences in host responses are associated with the ontogeny of the immunologic system.

scholarly journals Establishment and characterization of an Epstein-Barr virus transformed cell line with strong phagocytic activity

Blood ◽  
1989 ◽  
Vol 74 (1) ◽  
pp. 430-436 ◽  
Author(s):  
P von den Driesch ◽  
R Bhardwaj ◽  
HD Flad ◽  
DC Neugebauer ◽  
HJ Pielken ◽  
...  
Keyword(s):  
Cell Line ◽  
Phagocytic Activity ◽  
Antigen Processing ◽  
Epstein Barr Virus ◽  
Interleukin 1 ◽  
Immunoglobulin M ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
Transformed Cell Line ◽  
Epstein Barr

Abstract An immunoglobulin M (IgM)-positive cell line, Ms 28, apparently spontaneously transformed by Epstein-Barr virus (EBV) was established from peripheral blood cells of a patient with immature myeloblastic leukemia. It has been characterized according to phenotype, cytochemistry, and membrane antigen pattern. The cell line expresses lymphoid markers like CD 19, CD 22, and CD 30 and synthesizes and secretes IgM. Monocyte markers CD 11c, CD 14, and CD 15 are absent. Neither interleukin-1 (IL-1), nor tumor necrosis factor (TNF-alpha) are produced. But Ms 28 cells show strong phagocytic activity and engulf Latex particles and sheep RBCs (SRBCs) that need not to be opsonized. The phagocytic activity can be inhibited by chloroquine. Both phagocytosis and EBV nuclear-antigen (EBNA) expression can be observed in one and the same cell. Ms 28 cells might be useful to study immunologic activities like antigen processing and presentation.

The Biochemistry of Bereavement: Possible Basis for Chemotherapy?

1983 ◽  
Vol 13 (4) ◽  
pp. 295-303 ◽  
Author(s):  
Jerome F. Fredrick
Keyword(s):  
Infectious Disease ◽  
Epstein Barr Virus ◽  
Herpes Virus ◽  
Barr Virus ◽  
Adrenal Axis ◽  
Epstein Barr ◽  
Herpès Virus ◽  
Pituitary Adrenal Axis ◽  
Increased Susceptibility

The effect of acute grief on the pituitary-adrenal axis and the hypersecretion of cortisol is reviewed. Particular attention is devoted to the immunosuppressive effects of this hypersecretion. The increased susceptibility to infectious disease agents—bacterial, fungal and viral—is explored in terms of the altered biochemistry of the bereaved individual. The probable increased susceptibility towards oncogenic “passenger” viruses, such as the Epstein-Barr Virus and the Herpes Virus, is discussed as a possible mechanism for the increased incidence of malignancies in the bereaved. The use of dexamethasone in preventing hypersecretion of cortisol in the bereaved, as well as the use of L-dopa and clonidine to control A.C.T.H. levels in such individuals, has given rise to much conflicting data in recent studies. The use of antibiotics in a prophylactic sense, to bolster falling immunity during the grief period, remains a distinct possibility. However, until the basic reactions of grief are defined and the altered biochemistry established, it appears that psychological methods offer the best therapeutic means.

scholarly journals Occurrence of Immunoglobulin M Antibodies against Several Bacterial and Viral Pathogens in Acute Hantavirus Infection

2012 ◽  
Vol 19 (9) ◽  
pp. 1549-1551
Author(s):  
Nele Wellinghausen ◽  
Andrea Goetz ◽  
Ursula Weber
Keyword(s):  
Epstein Barr Virus ◽  
Immunoglobulin M ◽  
Hantavirus Infection ◽  
Puumala Virus ◽  
Virus Infections ◽  
Viral Pathogens ◽  
Content Type ◽  
Barr Virus ◽  
Epstein Barr ◽  
Type Gene

ABSTRACTElevated levels of immunoglobulin M antibodies against various pathogens, most frequently Epstein-Barr-virus andCoxiella burnetii, were detected by immunoassay in 15 of 48 patients (31.3%) with acute Puumala virus infections. Although the mechanisms leading to this IgM response are not clear yet, polyspecific immunoglobulin M antibodies have to be taken into account to avoid misinterpretation of serological results in acute hantavirus infection.

Epstein-barr virus and cytomegalovirus infections cause false-positive results in IgM two-test protocol for early lyme borreliosis

Infection ◽  
1999 ◽  
Vol 27 (3) ◽  
pp. 231-231 ◽  
Author(s):  
H. A. T. Goossens ◽  
A. E. J. M. van den Bogaard ◽  
M. K. E. Nohlmans ◽  
A. E. J. M. van den Bogaard
Keyword(s):  
Lyme Borreliosis ◽  
False Positive ◽  
Epstein Barr Virus ◽  
Test Protocol ◽  
Barr Virus ◽  
Epstein Barr ◽  
Cytomegalovirus Infections ◽  
Positive Results

scholarly journals Comparison of Three Automated Immunoassay Methods for the Determination of Epstein-Barr Virus-Specific Immunoglobulin M

2010 ◽  
Vol 17 (4) ◽  
pp. 559-563 ◽  
Author(s):  
Mario Berth ◽  
Eugene Bosmans
Keyword(s):  
Epstein Barr Virus ◽  
Antibody Test ◽  
Immunoglobulin M ◽  
Nuclear Antigen ◽  
Two Phase ◽  
Barr Virus ◽  
Epstein Barr ◽  
Automated Immunoassay ◽  
A Prospective Study

ABSTRACT In this study we compared the performances of three commercially available Epstein-Barr virus (EBV) immunoglobulin M (IgM) assays on highly automated immunoassay platforms: BioPlex 2200 (Bio-Rad Laboratories), Immulite 2000 (Siemens Healthcare Diagnostics), and Liaison (DiaSorin). As a confirmatory method, immunoblotting was performed. The specificity of the three EBV IgM assays was evaluated by testing 293 selected sera from patients with various infectious and noninfectious diseases. After the exclusion of 30 samples, the specificities were 96.2% for Liaison, 98.1% for Immulite, and 97.0% for BioPlex. For evaluation of the sensitivity, samples from 70 consecutive patients with a positive heterophile antibody test were examined, irrespective of clinical or biological findings. After the exclusion of six samples, the sensitivities were 89.1% for Liaison, 84.4% for Immulite, and 89.1% for BioPlex. Finally, in a prospective study performed with 500 samples obtained from consecutive patients and sent in by general practitioners, we also determined Epstein-Barr nuclear antigen IgG and viral capsid antigen IgG in a two-phase approach. Concordance of the EBV serologic status was 96.2% between Liaison and Immulite, 96.4% between Immulite and BioPlex, and 97.8% between BioPlex and Liaison. The three EBV IgM immunoassays that we evaluated have acceptable and comparable performances.

scholarly journals The effect of ingaron on the dynamics of copies of deoxyribonucleic acid Epstein - Barr virus in saliva samples and on the manifestation of clinical

2019 ◽  
Vol 21 (2) ◽  
pp. 18-23
Author(s):  
I A Rakityanskaya ◽  
T S Ryabova ◽  
U A Todzhibaev ◽  
A A Kalashnikov ◽  
A S Manuilov
Keyword(s):  
Epstein Barr Virus ◽  
Deoxyribonucleic Acid ◽  
Chain Reaction ◽  
Barr Virus ◽  
Negative Results ◽  
Significant Positive Change ◽  
Effect Of Treatment ◽  
Epstein Barr ◽  
Group 2 ◽  
Positive Results

A total of 60 patients (38 women and 22 men, 36,54±2,14 years old) with a Epstein - Barr infection were examined. The patients were divided into two groups depending on the treatment regimen: 1 group - 34 patients received ingaron therapy (10 intramuscular injections of 500000 units every other day); group 2-26 patients received valcyte (450 mg 2 times a day, by mouth, 2 months) + ingaron at the same dosage. One month after the end of antiviral therapy in the1st group, negative results of half-chain reaction were obtained only in 29,41% of patients. The combination of valcyte and ingaron in patients of the 2nd group was different and differed in the duration of the injection of ingarone: 2a group: ingaron10 injections of 500000 IU intramuscularly every other day; 2b group: ingaron 20 injections of 500000 IU intramuscularly every other day; 2c group: ingaron 10 injections of 500000 IU and 15 injections of 100000 IU intramuscularly every other day. The best result after the introduction of ingaron was obtained in 90% of patients who received the longest course of ingaron in the form of 20 injections of 500000 IU every other day in combination with valcyte. In the other groups, where the total dose of ingaron was lower, positive results were found in 60 and 66,7% of patients. A significant positive change in the number of copies of deoxyribonucleic acid Epstein - Barr virus was found in samples of saliva and the severity of clinical complaints in patients one month after the ingaron monotherapy or combination therapy (valcyte and ingaron) was completed. The best effect of treatment with ingaron is achieved in patients with chronic Epstein - Barr infection with the prolonged introduction of ingaron (at least 20 injections).

scholarly journals Clinical and laboratory features of infectious mononucleosis caused by the Epstein-Barr virus

2014 ◽  
Vol 95 (5) ◽  
pp. 722-725 ◽  
Author(s):  
R A Gileva ◽  
Z A Hohlova ◽  
Yu S Chechet ◽  
A S Arsenenko ◽  
L M Povolotskaya
Keyword(s):  
G Proteins ◽  
Infectious Mononucleosis ◽  
High Frequency ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Severe Form ◽  
Immunoglobulin M ◽  
Standard Laboratory ◽  
Barr Virus ◽  
Epstein Barr

Aim. To study the clinical and laboratory characteristics of patients with infectious mononucleosis caused by the Epstein-Barr virus. Methods. In infectious hospital under observation there were 108 patients aged from 11 months to 31 years with a laboratory confirmed diagnosis of «infectious mononucleosis caused by the Epstein-Barr virus». Standard laboratory tests for blood serum immunoglobulin M to the capsid protein of Epstein-Barr virus, immunoglobulin class G proteins to early Epstein-Barr virus and the index of their avidity by ELISA were carried out. Tests for other infections were conducted if it is required. Results. Patients dominated by children aged 1-7 years - 73.1%. Adult patients was 1.8% (2 patients). Only 4.6% of the patients were sent to the hospital with a diagnosis of «infectious mononucleosis» or suspected of having the disease. The typical clinical picture of infectious mononucleosis was observed in 75% of cases, atypical - in 25%. Mononuclear cells in peripheral blood were found in less than half of the patients (44.4%), in 53.1% of cases with typical and in 18.5% of cases with atypical clinic. In the remaining patients, the diagnosis of infectious mononucleosis was verified by ELISA. In 90.4% of the cases the disease was in the moderate, and in 9.6% - in the severe form. Conclusion. The high frequency of atypical course of infectious mononucleosis caused by the Epstein-Barr virus justifies the need to include it into differential diagnosis process in case of unclear infectious disease, to identify the antibodies to the Epstein-Barr virus proteins, (immunoglobulin M and G) and the index of avidity antibodies (immunoglobulin G).

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