scholarly journals Complete Genome Sequence of a Porcine Epidemic Diarrhea Virus Strain from Vietnam, HUA-14PED96, with a Large Genomic Deletion

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Se-Eun Choe ◽  
Kee-Hwan Park ◽  
Seong-In Lim ◽  
Van Phan Le ◽  
Nguyen Ba Hien ◽  
...  
Keyword(s):  
Complete Genome Sequence ◽  
Porcine Epidemic Diarrhea Virus ◽  
Virulent Strain ◽  
Open Reading Frame ◽  
High Identity ◽  
Reading Frame ◽  
Diarrhea Virus ◽  
Severe Diarrhea ◽  
Porcine Epidemic Diarrhea ◽  
Highly Virulent

A highly virulent strain of Porcine epidemic diarrhea virus (PEDV) causing severe diarrhea has recently emerged in Vietnam. Genomic sequences from a novel strain, HUA-14PED96, isolated from a Vietnamese piglet with serious diarrhea show relatively high identity with U.S.-like PEDV strains, and have a 72-nt deletion in the open reading frame 1a (ORF1a) gene.

scholarly journals Complete Genome Sequence of Variant Porcine Epidemic Diarrhea Virus Strain ZJ/ZX2018-C10, Isolated in Zhejiang, China, in 2018

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Fei Su ◽  
Bin Yu ◽  
Junxing Li ◽  
Lihua Xu ◽  
Xiufang Yuan
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Porcine Epidemic Diarrhea Virus ◽  
Complete Genome ◽  
Zhejiang Province ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Highly Virulent

We report here the complete genome sequence of porcine epidemic diarrhea virus (PEDV) strain ZJ/ZX2018-C10, isolated from infected piglets in Zhejiang Province, China. The genome sequence was highly similar to AH2012, a highly virulent Chinese PEDV strain.

scholarly journals The S Gene Is Necessary but Not Sufficient for the Virulence of Porcine Epidemic Diarrhea Virus Novel Variant Strain BJ2011C

2018 ◽  
Vol 92 (13) ◽  
Author(s):  
Di Wang ◽  
Xinna Ge ◽  
Dongjie Chen ◽  
Jie Li ◽  
Yueqi Cai ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Vaccine Development ◽  
Economic Losses ◽  
Avirulent Strain ◽  
Diarrhea Virus ◽  
Severe Diarrhea ◽  
S Gene ◽  
Viral Virulence ◽  
Porcine Epidemic Diarrhea ◽  
Highly Virulent

ABSTRACT The recently emerged highly virulent variants of porcine epidemic diarrhea virus (PEDV) have caused colossal economic losses to the worldwide swine industry. In this study, we investigated the viral virulence determinants by constructing a series of chimeric mutants between the highly virulent strain BJ2011C and the avirulent strain CHM2013. When tested in the 2-day-old piglet model, wild-type (WT) BJ2011C caused severe diarrhea and death of the piglets within 72 h. In contrast, its chimeric derivative carrying the S gene from CHM2013 (BJ2011C-S CHM ) was avirulent to the piglets. Moreover, reciprocal substitution of the BJ2011C S gene (CHM2013-S BJ ) did not enable CHM2013 to gain any virulence. However, when the whole structural protein-coding region of BJ2011C (CHM2013-SP BJ ) was swapped, CHM2013 started to gain the ability to efficiently colonize the intestinal tract and caused diarrhea in piglets. A further gain of virulence required additional acquisition of the 3′ untranslated region (UTR) of BJ2011C, and the resultant virus (CHM2013-SP + 3UTR BJ ) caused more severe diarrhea and death of piglets. Together, our findings suggest that the virulence of PEDV epidemic strains is a multigenic event and that the S gene is only one of the necessary determinants. IMPORTANCE The recently emerged highly virulent PEDV variants are the major cause of the global porcine epidemic diarrhea (PED) pandemic. The S gene of the variants undergoes remarkable variations and has been thought to be the virulence determinant for the enhanced pathogenesis. Our studies here showed that the S gene is only part of the story and that full virulence requires cooperation from other genes. Our findings provide insight into the pathogenic mechanism of the highly virulent PEDV variants and have implications for future vaccine development.

scholarly journals Evaluation of the Efficacy of Lactogenic Immunity of Sow Induced by Porcine Epidemic Diarrhea Virus (PEDV) Vaccine By Intranasal Immunization

2021 ◽  
Author(s):  
Shengchao Deng ◽  
Juan Wang ◽  
Mudassar Mohiuddin ◽  
Lisai Zhu ◽  
Guiping Wang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Protective Efficacy ◽  
Virulent Strain ◽  
Passive Immunization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diarrhea Virus ◽  
Suckling Piglets ◽  
Porcine Epidemic Diarrhea ◽  
Lactogenic Immunity ◽  
Highly Virulent

Abstract Background: Porcine epidemic diarrhea virus belongs to family of coronaviruses which are notorious for rapid spread of severe diarrhea among suckling piglets. The virus mainly replicates in the epithelial cells of duodenum, jejunum, ileum and colon and is a life threatening condition in pigs. A highly virulent strain “CHYJ130330” having high mortality rate was isolated from a field outbreak, identified as a new virulent genotype II/G2-b strain and adapted successfully to vero cells was used to prepare inactivated vaccine against PEDV. This newly prepared vaccine was given through intranasal route and is compared with the commercially available bi-combined (PEDV and TGEV) vaccine given by intramuscular injection. In this study milk or mucosal IgA and IgG antibody levels have been used to predict vaccine efficacy and the level of protective immunity against PED virus. Antibody titers in the milk of sows and intestines of suckling piglets were compared by enzyme-linked immunosorbent assay (ELISA). Results: It was shown that CHYJ vaccine induced significantly higher levels of PEDV IgA antibody in milk of sows and intestines of piglets as compared to commercial bi-combined vaccine. Both CHYJ and commercial vaccines were not able to induce detectable IgG levels in the intestines of piglets; the later however induced higher IgG levels when detected in the sow’s milk. Protective efficacy of vaccines was determined against a highly virulent PEDV strain. CHYJ intranasal vaccine gives a better protection 80% (4/5) rate as compared to commercial i.m. vaccine conferring 60% (3/5) immunity in suckling piglets. Conclusions: It is therefore concluded that PEDV inactivated CHYJ vaccine confer better lactogenic immunity and gives more protection to suckling piglets than available bi-combined TGEV and PEDV vaccine through passive immunization.

scholarly journals Complete Genome Sequences of Porcine Epidemic Diarrhea Virus Strains JSLS-1/2015 and JS-2/2015 Isolated from China

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Jie Tao ◽  
Benqiang Li ◽  
Chunling Zhang ◽  
Huili Liu
Keyword(s):  
Amino Acid ◽  
Porcine Epidemic Diarrhea Virus ◽  
Open Reading Frame ◽  
Watery Diarrhea ◽  
Amino Acid Deletion ◽  
Genome Sequences ◽  
Reading Frame ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Virus Strains

Two porcine epidemic diarrhea virus (PEDV) strains, JSLS-1/2015 and JS-2/2015, were isolated from piglets with watery diarrhea in South China. Two genomic sequences were highly homologous to the attenuated DR13 strain. Furthermore, JSLS-1/2015 contains a 24-amino-acid deletion in open reading frame 1b, which was first reported in PEDV isolates.

scholarly journals Complete Genome Sequence of a Recombinant Porcine Epidemic Diarrhea Virus Strain, CH/JXJA/2017, Isolated in Jiangxi, China, in 2017

2018 ◽  
Vol 6 (6) ◽  
pp. e01590-17 ◽  
Author(s):  
Kai Li ◽  
Deping Song ◽  
Fanfan Zhang ◽  
Wang Gong ◽  
Nannan Guo ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Porcine Epidemic Diarrhea Virus ◽  
Complete Genome ◽  
Recombinant Strain ◽  
Diarrhea Virus ◽  
Insertion Sites ◽  
Porcine Epidemic Diarrhea ◽  
Full Length Genome ◽  
Highly Virulent

ABSTRACT The full-length genome sequence of a variant of porcine epidemic diarrhea virus (PEDV), that of strain CH/JXJA/2017, was highly homologous to CH/ZMDZY/11, a highly virulent Chinese PEDV strain. CH/JXJA/2017 had a distant relationship with the attenuated CV777 vaccine strain, but the insertion sites of the S1 gene were similar to those of the recombinant strain of CH/ZMDZY/11.

scholarly journals Investigation of the Role of the Spike Protein in Reversing the Virulence of the Highly Virulent Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strains and Its Attenuated Counterpart

Viruses ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 41 ◽  
Author(s):  
Chi-Fei Kao ◽  
Hui-Wen Chang
Keyword(s):  
Mortality Rate ◽  
Porcine Epidemic Diarrhea Virus ◽  
Rational Design ◽  
Economic Losses ◽  
Diarrhea Virus ◽  
Viral Shedding ◽  
S Gene ◽  
Porcine Epidemic Diarrhea ◽  
Highly Virulent

Porcine epidemic diarrhea virus (PEDV) has continuously caused severe economic losses to the global swine industries; however, no successful vaccine against PEDV has been developed. In this study, we generated four autologous recombinant viruses, including the highly virulent iPEDVPT-P5, attenuated iPEDVPT-P96, and two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) with the reciprocally exchanged spike (S) gene, to study the role of the S gene in PEDV pathogenesis. A deeper understanding of PEDV attenuation will aid in the rational design of a live attenuated vaccine (LAV) using reverse genetics system. Our results showed that replacing the S gene from the highly virulent iPEDVPT-P5 led to complete restoration of virulence of the attenuated iPEDVPT-P96, with nearly identical viral shedding, diarrhea pattern, and mortality rate as the parental iPEDVPT-P5. In contrast, substitution of the S gene with that from the attenuated iPEDVPT-P96 resulted in partial attenuation of iPEDVPT-P5, exhibiting similar viral shedding and diarrhea patterns as the parental iPEDVPT-P96 with slightly severe histological lesions and higher mortality rate. Collectively, our data confirmed that the attenuation of the PEDVPT-P96 virus is primarily attributed to mutations in the S gene. However, mutation in S gene alone could not fully attenuate the virulence of iPEDVPT-P5. Gene (s) other than S gene might also play a role in determining virulence.

scholarly journals Full-Length Genome Sequence of Porcine Epidemic Diarrhea Virus Strain CH/GX/2015/750A

2017 ◽  
Vol 5 (27) ◽  
Author(s):  
Yibin Qin ◽  
Bingxia Lu ◽  
Ying He ◽  
Bin Li ◽  
Qunpeng Duan ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Porcine Epidemic Diarrhea Virus ◽  
Complete Genome ◽  
Full Length ◽  
Watery Diarrhea ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Full Length Genome

ABSTRACT We report here the complete genome sequence of porcine epidemic diarrhea virus (PEDV) strain CH/GX/2015/750A (750A), which was isolated from a suckling piglet with watery diarrhea in Guangxi, China. The isolate is genetically close to other recent Chinese variant PEDVs and distinct from the classical PEDVs.

scholarly journals Molecular Characteristics and Pathogenicity of Porcine Epidemic Diarrhea Virus Isolated in Some Areas of China in 2015–2018

2020 ◽  
Vol 7 ◽  
Author(s):  
Linyang Yu ◽  
Yanling Liu ◽  
Shuangyun Wang ◽  
Leyi Zhang ◽  
Pengshuai Liang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Vero Cells ◽  
Economic Losses ◽  
Molecular Characteristics ◽  
Phylogenetic Tree Analysis ◽  
Genetic Characteristics ◽  
Diarrhea Virus ◽  
Severe Diarrhea ◽  
Positive Rate ◽  
Porcine Epidemic Diarrhea

Since 2010, Porcine epidemic diarrhea virus (PEDV) has caused severe diarrhea disease in piglets in China, resulting in large economic losses. To understand the genetic characteristics of the PEDV strains that circulated in some provinces of China between 2015 and 2018, 375 samples of feces and small intestine were collected from pigs and tested. One hundred seventy-seven samples tested positive and the PEDV-positive rate was 47.20%. A phylogenetic tree analysis based on the entire S gene showed that these strains clustered into four subgroups, GI-a, GI-b, GII-a, and GII-b, and that the GII-b strains have become dominant in recent years. Compared with previous strains, these strains have multiple variations in the SP and S1-NTD domains and in the neutralizing epitopes of the S protein. We also successfully isolated and identified a new virulent GII-b strain, GDgh16, which is well-adapted to Vero cells and caused a high mortality rate in piglets in challenge experiments. Our study clarifies the genetic characteristics of the prevalent PEDV strains in parts of China, and suggests that the development of effective novel vaccines is both necessary and urgent.

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