scholarly journals Complete and Assembled Genome Sequence of Staphylococcus aureus RKI4, a Food-Poisoning Strain Exhibiting a Novel S. aureus Pathogenicity Island Carrying seb

2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Marc J. A. Stevens ◽  
Roger Stephan ◽  
Sophia Johler
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Food Poisoning ◽  
Pathogenicity Island

scholarly journals Comparative Genomic Analysis of Fifty-Two Staphylococcus aureus Isolates Identified from Uncharacterized Staphylococcus Genomes in the NCBI database

2020 ◽  
Author(s):  
Mohamed A. Abouelkhair
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Food Poisoning ◽  
Bacterial Pathogen ◽  
Variant Calling ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Genome Sequences ◽  
Spa Typing ◽  
Genome Level

AbstractBackgroundStaphylococcus aureus is a major bacterial pathogen that causes a variety of diseases, ranging from wound infections to severe bacteremia or food poisoning. The course and severity of the disease are mainly dependent on the bacterium genotype as well as host factors. Whole-genome sequencing (WGS) is currently the most extensive genotyping method available, followed by bioinformatic sequence analysis.MethodsA total of 253 uncharacterized staphylococcus genome sequences were downloaded from the National Center for Biotechnology Information (NCBI) (August 2012 to March 2020) from different studies. Samples were clustered based on core and accessory pairwise distances between isolates and then analyzed by multilocus sequence typing tool (MLST). Staphylococcal Cassette Chromosome mec (SCCmec), spa typing, variant calling, core genome alignment, and recombination sites prediction were performed on detected S. aureus isolates. S. aureus isolates were also analyzed for the presence of genes coding for virulence factors and antibiotic resistance.Results and conclusionUncategorized genome sequences were clustered into 24 groups. About 182 uncharacterized Staphylococcus genomes were identified at the species level based on MLST, including 32 S. lugdunensis genome sequence, thus doubling the number of the publicly accessible S. lugdunensis genome sequence in Genbank. MLST identified another four species (S. epidermidis (33/253), S. lugdunensis (32/253), S. haemolyticus (41/253), S. hominis (24/253) and S. aureus (52/253)). Among the 52 S. aureus isolates, 21 (40.38%) isolates carried mecA gene, with 57.14% classified as SCCmec IV. The results of this study provide knowledge that facilitates evolutionary studies of staphylococcal species and other bacteria at the genome level.

scholarly journals Complete Sequence of a Staphylococcus aureus Clonal Complex 81 Strain, the Dominant Lineage in Food Poisoning Outbreaks in Japan

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Yusuke Sato’o ◽  
Junzo Hisatsune ◽  
Hideki Hirakawa ◽  
Hisaya K. Ono ◽  
Katsuhiko Omoe ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Clonal Complex ◽  
Food Poisoning ◽  
Complete Sequence ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Content Type

ABSTRACT Staphylococcus aureus No. 10 is an isolate from a staphylococcal food poisoning outbreak in Japan, classified as clonal complex 81 subtype 1. It preferentially produces larger quantities of staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin H (SEH) in foods and media. Here, we report the complete annotated genome sequence of the chromosome and a plasmid.

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

scholarly journals Complement Inactivation Strategy of Staphylococcus aureus Using Decay-Accelerating Factor and the Response of Infected HaCaT Cells

2021 ◽  
Vol 22 (8) ◽  
pp. 4015
Author(s):  
Kyoung Ok Jang ◽  
Youn Woo Lee ◽  
Hangeun Kim ◽  
Dae Kyun Chung
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Cells ◽  
Respiratory Infections ◽  
Food Poisoning ◽  
Surface Expression ◽  
Host Cells ◽  
Hacat Cells ◽  
Decay Accelerating Factor ◽  
Ligand Expression ◽  
The Complement System

Staphylococcus aureus is a species of Gram-positive staphylococcus. It can cause sinusitis, respiratory infections, skin infections, and food poisoning. Recently, it was discovered that S. aureus infects epithelial cells, but the interaction between S. aureus and the host is not well known. In this study, we confirmed S. aureus to be internalized by HaCaT cells using the ESAT-6-like protein EsxB and amplified within the host over time by escaping host immunity. S. aureus increases the expression of decay-accelerating factor (CD55) on the surfaces of host cells, which inhibits the activation of the complement system. This mechanism makes it possible for S. aureus to survive in host cells. S. aureus, sufficiently amplified within the host, is released through the initiation of cell death. On the other hand, the infected host cells increase their surface expression of UL16 binding protein 1 to inform immune cells that they are infected and try to be eliminated. These host defense systems seem to involve the alteration of tight junctions and the induction of ligand expression to activate immune cells. Taken together, our study elucidates a novel aspect of the mechanisms of infection and immune system evasion for S. aureus.

scholarly journals Whole-Genome Sequence for Methicillin-Resistant Staphylococcus aureus Strain ATCC BAA-1680

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Luke T. Daum ◽  
Violet V. Bumah ◽  
Daniela S. Masson-Meyers ◽  
Manjeet Khubbar ◽  
John D. Rodriguez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome ◽  
Strain Atcc ◽  
Methicillin Resistant

Bacterial community of castor plants growing on municipal solid waste dumpsites of Wukari metropolis

2021 ◽  
Vol 6 (1) ◽  
pp. 1-6
Author(s):  
Anongo, M’ember Catherine ◽  
Ibrahim Jamilat Mijinyawa
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Community ◽  
Ricinus Communis ◽  
Alcoholic Beverage ◽  
Food Poisoning ◽  
Ecological Factors ◽  
Suitable Habitat ◽  
Frozen Food ◽  
Hospital Site ◽  
Ricinus Communis L

Ricinus communis L. are usually found growing abundantly in wastelands where MSW are disposed in urban and semi-urban settlements. The aim of the research is to ascertain the ecological factors that influence the proliferation of Castor bean plants Ricinus communis L. on MSW dumpsites. A study was conducted between the months of December 2018 to April 2019 on fourteen MSW dumpsites from fourteen selected localities in Wukari Metropolis. Three localities were categorized into highly populated (Hospital site, Marmara 1 and Marmara 2) sites; six were moderately populated (GRA, New Site, T-junction, Avyi 1, Mission Quarters 1, Mission Quarters 2) and five (New Market, Kwararafa 1, Timber Shed, Rice Mill, Albaco Maiko) were sparsely populated sites. Results revealed that Hospital Site, Marmara 1and Marmara 2 exhibited the highest biochemical activities due to high population density and waste generation at the study sites. The bacterial community reveals high diversity of gram-positive (Lactobacillus, Bacillus and Staphylococcus aureus) and gram-negative (Pseudomonas and Klebsiella) due to the presence of large volumes of untreated sewage, household garbage, agricultural wastes/poultry farms/abbatoirs, frozen food shops, hospital wastes, fermented sorghum-an alcoholic beverage called “burukutu” and “nono” – fresh cowmilk in the MSW dumpsites. The presence of Klebsiella in the dumpsites reveals their ubiquity of belonging to the coliforms group and potential indicator organism as an index of possible water contamination and from hospital acquired infection including Staphylococcus aureus (food poisoning). The survival, growth and proliferation of Ricinus communis L. on the MSW dumpsites is dependent on its symbiotic relationship with the five Orders of bacteria that plays critical roles in organic matter synthesis, degradation, detoxification and novel metabolic abilities thus creates a suitable habitat.

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