Inhibitory effects of extracellular products from oral bacteria on human fibroblasts and stimulated lymphocytes.

Fibroproliferative diseases are characterized by excessive accumulation of extracellular matrix (ECM) components leading to organ dysfunction. This process is characterized by an increase in myofibroblast content and enzyme activity of xylosyltransferase-I (XT-I), the initial enzyme in proteoglycan (PG) biosynthesis. Therefore, the inhibition of XT-I could be a promising treatment for fibrosis. We used a natural product-inspired compound library to identify non-substrate-based inhibitors of human XT-I by UPLC-MS/MS. We combined this cell-free approach with virtual and molecular biological analyses to confirm and prioritize the inhibitory potential of the compounds identified. The characterization for compound potency in TGF-β1-driven XYLT1 transcription regulation in primary dermal human fibroblasts (key cells in ECM remodeling) was addressed by gene expression analysis. Consequently, we identified amphotericin B and celastrol as new non-substrate-based XT-I protein inhibitors. Their XT-I inhibitory effects were mediated by an uncompetitive or a competitive inhibition mode, respectively. Both compounds reduced the cellular XYLT1 expression level and XT-I activity. We showed that these cellular inhibitor-mediated changes involve the TGF-β and microRNA-21 signaling pathway. The results of our study provide a strong rationale for the further optimization and future usage of the XT-I inhibitors identified as promising therapeutic agents of fibroproliferative diseases.

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Inhibitory Effects on Selected Oral Bacteria of Antibacterial Agents Incorporated in a Glass Ionomer Cement

Caries Research ◽

10.1159/000069019 ◽

2003 ◽

Vol 37 (2) ◽

pp. 108-114 ◽

Cited By ~ 49

Author(s):

Michael G. Botelho

Keyword(s):

Antibacterial Agents ◽

Glass Ionomer Cement ◽

Oral Bacteria ◽

Glass Ionomer ◽

Inhibitory Effects ◽

Ionomer Cement

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Inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria

Dental Materials ◽

10.1016/j.dental.2014.04.007 ◽

2014 ◽

Vol 30 (9) ◽

pp. e238-e244 ◽

Cited By ~ 13

Author(s):

Éfani C.F. Banzi ◽

Ana R. Costa ◽

Regina M. Puppin-Rontani ◽

Jegdish Babu ◽

Franklin García-Godoy

Keyword(s):

Metabolic Activity ◽

Oral Bacteria ◽

Inhibitory Effects ◽

Bonding System

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Inhibitory effect of toothbrush monofilament containing surface pre-reacted glass-ionomer (S-PRG) filler on Streptococcus mutans

Scientific Reports ◽

10.1038/s41598-020-80646-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Saaya Matayoshi ◽

Ryota Nomura ◽

Takahiro Kitamura ◽

Rena Okawa ◽

Kazuhiko Nakano

Keyword(s):

Oral Health ◽

Dental Caries ◽

Streptococcus Mutans ◽

Dental Materials ◽

Self Care ◽

Inhibitory Effect ◽

Oral Bacteria ◽

Glass Ionomer ◽

Inhibitory Effects ◽

Oral Environment

AbstractThe oral environment affects not only oral health, but also general health, and the importance of oral self-care has recently been recognised. Although toothbrushes are the most important self-care product, there are few toothbrushes that have an inhibitory effect on oral bacteria. In the present study, monofilaments used for toothbrushes containing surface pre-reacted glass-ionomer (S-PRG) filler (a component recently applied to various dental materials) were developed. Among nylon and polyester monofilaments commonly used for toothbrushes, nylon monofilaments can accommodate more S-PRG filler than polyester monofilaments, resulting in greater release of ions from the S-PRG filler. These monofilaments containing S-PRG filler formed less biofilm containing Streptococcus mutans, a major pathogen of dental caries, than monofilaments without S-PRG filler. Moreover, S. mutans adhering to monofilaments containing S-PRG filler were more easily exfoliated and eliminated than those adhering to monofilaments without S-PRG filler. Such inhibitory effects on S. mutans were more marked in nylon monofilaments than in polyester monofilaments. These findings that monofilaments containing S-PRG filler can release ions and have an inhibitory effect on S. mutans suggest that they may be an effective material for toothbrushes.

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Effect of silica nano-spheres on adhesion of oral bacteria and human fibroblasts

Biomaterial Investigations in Dentistry ◽

10.1080/26415275.2020.1816175 ◽

2020 ◽

Vol 7 (1) ◽

pp. 134-145

Author(s):

Pawel Kallas ◽

Hua Kang ◽

Håkon Valen ◽

Håvard Jostein Haugen ◽

Martin Andersson ◽

...

Keyword(s):

Human Fibroblasts ◽

Oral Bacteria

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Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D

Microbial Cell Factories ◽

10.1186/s12934-021-01563-w ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Soyon Mann ◽

Myeong Soo Park ◽

Tony V. Johnston ◽

Geun Eog Ji ◽

Keum Taek Hwang ◽

...

Keyword(s):

Cell Adhesion ◽

Starter Culture ◽

Oral Bacteria ◽

Inhibitory Effects ◽

Food Ingredient ◽

Anaerobic Microorganisms ◽

Oral Pathogens ◽

Displacement Assays ◽

Probiotic Microorganism ◽

Oral Microorganisms

Abstract Background Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body. Results One organism was isolated, named “L. gasseri HHuMIN D”, and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. Conclusion These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

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Growth inhibitory effects of ATP and its derivatives on human fibroblasts immortalized with 60Co-gamma rays.

International Journal of Molecular Medicine ◽

10.3892/ijmm.5.1.59 ◽

2000 ◽

Author(s):

J W Li ◽

Y Inoue ◽

M Miyazaki ◽

H Pu ◽

A Kondo ◽

...

Keyword(s):

Gamma Rays ◽

Human Fibroblasts ◽

Inhibitory Effects ◽

Growth Inhibitory ◽

60Co Gamma ◽

60Co Gamma Rays

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The regulation of proteolysis in normal fibroblasts as they approach confluence. Evidence for the participation of the lysosomal system

Biochemical Journal ◽

10.1042/bj2080795 ◽

1982 ◽

Vol 208 (3) ◽

pp. 795-800 ◽

Cited By ~ 22

Author(s):

S M Cockle ◽

R T Dean

Keyword(s):

Proteinase Inhibitors ◽

Human Fibroblasts ◽

Growth Phases ◽

Inhibitory Effects ◽

Intracellular Proteins ◽

Lysosomotropic Agent ◽

Normal Human ◽

Lysosomal System ◽

Swiss 3T3

The effect of the lysosomotropic agent NH4Cl and the proteinase inhibitors leupeptin, Z-Phe-Ala-CHN2 (benzyloxycarbonylphenylalanylalanyldiazomethane) and pepstatin on the degradation of intracellular proteins in Swiss 3T3 mouse and normal human fibroblasts in both the exponential and stationary (confluent) growth phases in nutritionally complete conditions was investigated. Inhibitory effects of all four agents on degradation in both growth states were detected. The increase in proteolysis normally occurring as cells approach confluence could be completely blocked by NH4Cl, by Z-Phe-Ala-CHN2, or by pepstatin in the presence of leupeptin. These results suggest that the lysosomal system is responsible for the regulation of proteolysis at confluence and further confirm its role in ‘basal’ proteolysis in growing cells.

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Oral Probiotic Activities and Biosafety of Lactobacillus gasseri HHuMIN D

10.21203/rs.3.rs-144596/v1 ◽

2021 ◽

Author(s):

Soyon Mann ◽

Myeong Soo Park ◽

Tony V. Johnston ◽

Geun Eog Ji ◽

Keum Taek Hwang ◽

...

Keyword(s):

Cell Adhesion ◽

Starter Culture ◽

Oral Bacteria ◽

Inhibitory Effects ◽

Food Ingredient ◽

Genetic Characteristics ◽

Anaerobic Microorganisms ◽

Oral Bacterium ◽

Oral Pathogens ◽

Hemolytic Property

Abstract Background Specific probiotic bacteria may have inhibitory effects against oral pathogens. Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research aimed to determine the safety and inhibitory impacts of L. gasseri HHuMIN D as a potential oral probiotic with biotherapeutic capabilities against oral pathogens. Results The microbial and genetic characteristics of L. gasseri HHuMIN D were evaluated in this research. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and L. gasseri HHuMIN D exhibited powerful inhibitory effects on the growth of every harmful oral bacterium tested. Unfortunately, other oral bacteria affected the growth of L. gasseri HHuMIN D, suggesting a poor proliferative response to most co-cultures. Hydrogen peroxide production by L. gasseri HHuMIN D reached 802 µmol/L after 12 hours and gradually diminished until 24 hours. It efficiently aggregated with P. catoniae and S. sanguinis, and completely supressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. L. gasseri HHuMIN D was evaluated for safety using ammonia and biogenic amine development, hemolytic property and mucin degradation testing, antibiotic susceptibility, and whole genome sequencing (WGS). Conclusion Our results suggest that L. gasseri HHuMIN D may be a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

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