Inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria

AbstractThe oral environment affects not only oral health, but also general health, and the importance of oral self-care has recently been recognised. Although toothbrushes are the most important self-care product, there are few toothbrushes that have an inhibitory effect on oral bacteria. In the present study, monofilaments used for toothbrushes containing surface pre-reacted glass-ionomer (S-PRG) filler (a component recently applied to various dental materials) were developed. Among nylon and polyester monofilaments commonly used for toothbrushes, nylon monofilaments can accommodate more S-PRG filler than polyester monofilaments, resulting in greater release of ions from the S-PRG filler. These monofilaments containing S-PRG filler formed less biofilm containing Streptococcus mutans, a major pathogen of dental caries, than monofilaments without S-PRG filler. Moreover, S. mutans adhering to monofilaments containing S-PRG filler were more easily exfoliated and eliminated than those adhering to monofilaments without S-PRG filler. Such inhibitory effects on S. mutans were more marked in nylon monofilaments than in polyester monofilaments. These findings that monofilaments containing S-PRG filler can release ions and have an inhibitory effect on S. mutans suggest that they may be an effective material for toothbrushes.

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Inhibitory effects of extracellular products from oral bacteria on human fibroblasts and stimulated lymphocytes.

Infection and Immunity ◽

10.1128/iai.21.2.567-574.1978 ◽

1978 ◽

Vol 21 (2) ◽

pp. 567-574 ◽

Cited By ~ 13

Author(s):

T B Higerd ◽

D H Vesole ◽

J M Goust

Keyword(s):

Human Fibroblasts ◽

Oral Bacteria ◽

Inhibitory Effects ◽

Extracellular Products

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Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D

Microbial Cell Factories ◽

10.1186/s12934-021-01563-w ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Soyon Mann ◽

Myeong Soo Park ◽

Tony V. Johnston ◽

Geun Eog Ji ◽

Keum Taek Hwang ◽

...

Keyword(s):

Cell Adhesion ◽

Starter Culture ◽

Oral Bacteria ◽

Inhibitory Effects ◽

Food Ingredient ◽

Anaerobic Microorganisms ◽

Oral Pathogens ◽

Displacement Assays ◽

Probiotic Microorganism ◽

Oral Microorganisms

Abstract Background Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body. Results One organism was isolated, named “L. gasseri HHuMIN D”, and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. Conclusion These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

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The effect of cranberry juice and a cranberry functional beverage on the growth and metabolic activity of selected oral bacteria

BMC Oral Health ◽

10.1186/s12903-021-02025-w ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Paulina M. Nowaczyk ◽

Joanna Bajerska ◽

Małgorzata Lasik-Kurdyś ◽

Elżbieta Radziejewska-Kubzdela ◽

Artur Szwengiel ◽

...

Keyword(s):

Metabolic Activity ◽

Oral Bacteria ◽

Oral Disease ◽

Oral Microbiota ◽

Risk Indicator ◽

Oral Diseases ◽

Cranberry Juice ◽

Impedance Measurements ◽

Antimicrobial Potential ◽

Periodontal Inflammation

Abstract Background The oral microbiota is a significant risk indicator for oral diseases, such as dental caries and periodontal inflammation. Much attention is presently paid to the development of functional foods (e.g. beverages containing cranberry constituents, or foods containing probiotics) that may serve as adjuncts for oral disease treatments (e.g. periodontitis and caries). Cranberry fruit, due to its unique chemical composition and antimicrobial potential, is a possible ingredient of such foods. The study aimed to investigate the effects of cranberry juice (CJ) and a cranberry functional beverage (mixture of 80% v/v apple juice, 20% v/v cranberry juice, and 0.25 g/100 mL ground cinnamon; CFB) on the growth and metabolic activity of selected oral bacteria. Methods Serial dilution pour plate method (SDPP) was used to examine the effect of CJ and CFB on the growth of Actinomyces naeslundii, Streptococcus mutans, and Lactobacillus paracasei subsp. paracasei. 48-h electrical impedance measurements (EIM) during the cultivation of A. naeslundii were applied to evaluate the utility of the method as a rapid alternative for the assessment of the antimicrobial potential of cranberry beverages. Results The tested bacteria differed in their susceptibility to the antimicrobial action of CJ and CFB, with L. paracasei subsp. paracasei being least vulnerable to CFB (according to SDPP). Although CJ at a concentration of 0.5 mL/mL, showed a bactericidal effect on the growth of S. mutans, A. naeslundii was more sensitive to CJ (SDPP). Its inhibitory effect on A. naeslundii was seen even at concentrations as small as 0.03125–0.125 mL/mL (SDPP and EIM). On the other hand, S. mutans seemed to be more vulnerable to CFB than A. naeslundii (SDPP). Conclusions CFB may be considered an adjunct in the treatment of oral diseases due to its action against selected oral pathogens, and not against the presumably beneficial L. paracasei subsp. paracasei. Bioelectrical impedance measurements appear to be a quick alternative to evaluating the antimicrobial activity of fruit beverages, but their utility should be confirmed with tests on other bacteria.

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Functional Profiling Reveals Altered Metabolic Activity in Divers’ Oral Microbiota During Commercial Heliox Saturation Diving

Frontiers in Physiology ◽

10.3389/fphys.2021.702634 ◽

2021 ◽

Vol 12 ◽

Author(s):

Roxane Monnoyer ◽

Ingrid Eftedal ◽

Astrid Hjelde ◽

Sanjoy Deb ◽

Kjersti Haugum ◽

...

Keyword(s):

Metabolic Activity ◽

Stress Responses ◽

Metabolic Pathways ◽

Marker Gene ◽

Extreme Environment ◽

Oral Bacteria ◽

Antioxidant Defenses ◽

Metagenomic Data ◽

Oral Microbiota ◽

Induced Changes

Background: The extreme environment in saturation diving affects all life forms, including the bacteria that reside on human skin and mucosa. The oral cavity alone is home to hundreds of different bacteria. In this study, we examined the metabolic activity of oral bacteria from healthy males during commercial heliox saturation diving. We focused on environmentally induced changes that might affect the divers’ health and fitness.Methods: We performed pathway abundance analysis using PICRUSt2, a bioinformatics software package that uses marker gene data to compute the metabolic activity of microbial communities. The analysis is based on 16S rRNA metagenomic data generated from the oral microbiota of 23 male divers before, during, and after 4weeks of commercial heliox saturation diving. Environmentally induced changes in bacterial metabolism were computed from differences in predicted pathway abundances at baseline before, versus during, and immediately after saturation diving.Results and Conclusion: The analysis predicted transient changes that were primarily associated with the survival and growth of bacteria in oxygenated environments. There was a relative increase in the abundance of aerobic metabolic pathways and a concomitant decrease in anaerobic metabolic pathways, primarily comprising of energy metabolism, oxidative stress responses, and adenosylcobalamin biosynthesis. Adenosylcobalamin is a bioactive form of vitamin B12 (vitB12), and a reduction in vitB12 biosynthesis may hypothetically affect the divers’ physiology. While host effects of oral bacterial vitamin metabolism are uncertain, this is a finding that concurs with the existing recommendations for vitB12 supplements as part of the divers’ diet, whether to boost antioxidant defenses in bacteria or their host or to improve oxygen transport during saturation diving.

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Oral Probiotic Activities and Biosafety of Lactobacillus gasseri HHuMIN D

10.21203/rs.3.rs-144596/v1 ◽

2021 ◽

Author(s):

Soyon Mann ◽

Myeong Soo Park ◽

Tony V. Johnston ◽

Geun Eog Ji ◽

Keum Taek Hwang ◽

...

Keyword(s):

Cell Adhesion ◽

Starter Culture ◽

Oral Bacteria ◽

Inhibitory Effects ◽

Food Ingredient ◽

Genetic Characteristics ◽

Anaerobic Microorganisms ◽

Oral Bacterium ◽

Oral Pathogens ◽

Hemolytic Property

Abstract Background Specific probiotic bacteria may have inhibitory effects against oral pathogens. Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research aimed to determine the safety and inhibitory impacts of L. gasseri HHuMIN D as a potential oral probiotic with biotherapeutic capabilities against oral pathogens. Results The microbial and genetic characteristics of L. gasseri HHuMIN D were evaluated in this research. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and L. gasseri HHuMIN D exhibited powerful inhibitory effects on the growth of every harmful oral bacterium tested. Unfortunately, other oral bacteria affected the growth of L. gasseri HHuMIN D, suggesting a poor proliferative response to most co-cultures. Hydrogen peroxide production by L. gasseri HHuMIN D reached 802 µmol/L after 12 hours and gradually diminished until 24 hours. It efficiently aggregated with P. catoniae and S. sanguinis, and completely supressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. L. gasseri HHuMIN D was evaluated for safety using ammonia and biogenic amine development, hemolytic property and mucin degradation testing, antibiotic susceptibility, and whole genome sequencing (WGS). Conclusion Our results suggest that L. gasseri HHuMIN D may be a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

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