scholarly journals The Human Cationic Antimicrobial Protein (hCAP-18) Is Expressed in the Epithelium of Human Epididymis, Is Present in Seminal Plasma at High Concentrations, and Is Attached to Spermatozoa

2000 ◽  
Vol 68 (7) ◽  
pp. 4297-4302 ◽  
Author(s):  
Johan Malm ◽  
Ole Sørensen ◽  
Terese Persson ◽  
Margareta Frohm-Nilsson ◽  
Bengt Johansson ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Reproductive System ◽  
Male Reproductive System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antimicrobial Protein ◽  
Healthy Donors ◽  
Standard Deviation Range ◽  
Doublet Band ◽  
High Concentrations

ABSTRACT Innate immunity is important for the integrity of the host against potentially invasive pathogenic microorganisms in the environment. Antibiotic peptides with broad antimicrobial activity are part of the innate immune system. We investigated the presence of the cathelicidin, human cationic antimicrobial protein (hCAP-18), in the male reproductive system. We found strong expression of the hCAP-18 gene by in situ hybridization and hCAP-18 protein, as detected by immunohistochemistry, in the epithelium of the epididymis, but not in the testis. The highest expression in the epididymis was in the caudal part. Western blotting showed a doublet band, the upper part corresponding to the size of hCAP-18 in plasma and neutrophils. Using a specific enzyme-linked immunosorbent assay (ELISA), levels of 86.5 ± 37.8 μg/ml (mean ± standard deviation; range, 41.8 to 142.8 μg/ml; n = 10) were detected in seminal plasma from healthy donors, which is 70-fold higher than the level in blood plasma. Flow cytometry and immunocytochemistry revealed the presence of hCAP-18 on spermatozoa. ELISA measurement showed levels of 196 ng/106 spermatozoa, corresponding to 6.6 × 106 molecules of hCAP-18 per spermatozoon. Our results suggest a key role for hCAP-18 in the antibacterial integrity of the male reproductive system. The attachment of hCAP-18 to spermatozoa may implicate a role for hCAP-18 in conception.

scholarly journals Application and Progress of Raman Spectroscopy in Male Reproductive System

2022 ◽  
Vol 9 ◽  
Author(s):  
Feng Zhang ◽  
Yiling Tan ◽  
Jinli Ding ◽  
Dishuang Cao ◽  
Yanan Gong ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Seminal Plasma ◽  
Reproductive System ◽  
Biochemical Composition ◽  
Male Reproductive System ◽  
Non Invasive ◽  
Mesh Terms ◽  
Time Scanning ◽  
Non Destructive

Raman spectroscopy is a fast-developing, unmarked, non-invasive, non-destructive technique which allows for real-time scanning and sampling of biological samples in situ, reflecting the subtle biochemical composition alterations of tissues and cells through the variations of spectra. It has great potential to identify pathological tissue and provide intraoperative assistance in clinic. Raman spectroscopy has made many exciting achievements in the study of male reproductive system. In this review, we summarized literatures about the application and progress of Raman spectroscopy in male reproductive system from PubMed and Ovid databases, using MeSH terms associated to Raman spectroscopy, prostate, testis, seminal plasma and sperm. The existing challenges and development opportunities were also discussed and prospected.

Seminal plasma as a diagnostic fluid for male reproductive system disorders

2014 ◽  
Vol 11 (5) ◽  
pp. 278-288 ◽  
Author(s):  
Andrei P. Drabovich ◽  
Punit Saraon ◽  
Keith Jarvi ◽  
Eleftherios P. Diamandis
Keyword(s):  
Seminal Plasma ◽  
Reproductive System ◽  
Male Reproductive System

scholarly journals Expression of estrogen receptor-alpha and -beta mRNAs in the male reproductive system of the rat as revealed by in situ hybridization

2001 ◽  
Vol 26 (3) ◽  
pp. 165-174 ◽  
Author(s):  
CN Mowa ◽  
T Iwanaga
Keyword(s):  
Estrogen Receptor ◽  
In Situ Hybridization ◽  
Reproductive System ◽  
Initial Segment ◽  
Vas Deferens ◽  
Perinatal Period ◽  
Reproductive Organs ◽  
Male Reproductive System ◽  
Prenatal Period

We mapped the cellular expression of estrogen receptor (ER) alpha and ERbeta mRNAs in the male reproductive system of the rat during development and adulthood by in situ hybridization. The expression patterns of ERalpha mRNA in the gonad, efferent duct and initial segment of the epididymis during the perinatal period were essentially similar to those of the adult: ERalpha mRNA signals were expressed most intensely in the epithelia of the efferent ducts and initial segment of the epididymis, and in the interstitial cells of the testis from the prenatal period to adulthood. However, ERalpha mRNA signals in the primordial epididymis and vas deferens during the prenatal period were confined to the outermost cellular layer of the ducts, whereas thereafter they were only expressed weakly in the epithelium and stroma of the epididymis and moderately in the muscle layer of the vas deferens. ERbeta signals were expressed intensely (1) in primordial germ and Sertoli cells only during the prenatal period, (2) in arterial walls in the adult testis, and (3) in the epithelium of the sex accessory glands from the perinatal period to adulthood. This report is the first to describe the cellular distribution of ER mRNA in the male reproductive organs during the perinatal period, and complements and confirms earlier data on its distribution in the adult. The broad expression of ERs in male reproductive organs suggests roles for estrogen in regulating tissue development and reproductive events.

Inhibition of acrosin by protein C inhibitor and localization of protein C inhibitor to spermatozoa

1994 ◽  
Vol 267 (2) ◽  
pp. C466-C472 ◽  
Author(s):  
X. Zheng ◽  
M. Geiger ◽  
S. Ecke ◽  
E. Bielek ◽  
P. Donner ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Protein C ◽  
Reproductive Tract ◽  
Human Sperm ◽  
Order Rate Constant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amidolytic Activity ◽  
Protein C Inhibitor ◽  
Seminal Plasma Proteins ◽  
High Concentrations

Protein C inhibitor (PCI) is synthesized by cells throughout the male reproductive tract and is present in high concentrations (220 micrograms/ml) in seminal plasma. Seminal plasma as well as the acrosome of spermatozoa are rich in possible target proteases for PCI. We analyzed the interaction of PCI with acrosin, a serine protease stored in its zymogen form in the acrosome of spermatozoa. Purified human PCI inhibited the amidolytic activity of purified boar acrosin with an apparent second-order rate constant of 3.7 x 10(4) M-1.s-1. Inhibition was paralleled by the degradation of PCI from its 57- to its 54-kDa form. Human PCI also inhibited the amidolytic activity of activated human sperm extracts and formed complexes with acrosin as determined by an enzyme-linked immunosorbent assay. Immunocytochemistry revealed that morphologically abnormal spermatozoa stained for PCI antigen, whereas morphologically normal spermatozoa were negative. In immunoelectron microscopy, PCI was exclusively localized in the immediate vicinity of disrupted acrosomal membranes of sperm heads. These data suggest that PCI might function as a scavenger of prematurely activated acrosin, thereby protecting intact surrounding cells and seminal plasma proteins from possible proteolytic damage.

Calcium and Magnesium in Male Reproductive System and in Its Secretion. I. Level in Normal Human Semen, Seminal Plasma and Spermatozoa

2015 ◽  
Vol 82 (3) ◽  
pp. 174-178 ◽  
Author(s):  
James Valsa ◽  
Kalanghot Padmanabhan Skandhan ◽  
Pulikkal Sahab Khan ◽  
Kalanghot Padmanabhan Skandhan Avni ◽  
Skandhan Amith ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Reproductive System ◽  
Male Reproductive System ◽  
Human Semen ◽  
Normal Human ◽  
Calcium And Magnesium

scholarly journals Human leukocyte antigen-G in the male reproductive system and in seminal plasma

2011 ◽  
Vol 17 (12) ◽  
pp. 727-738 ◽  
Author(s):  
M. H. Larsen ◽  
M. Bzorek ◽  
M. B. Pass ◽  
L. G. Larsen ◽  
M. W. Nielsen ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Seminal Plasma ◽  
Reproductive System ◽  
Human Leukocyte ◽  
Male Reproductive System ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

New Technologies for Decontamination of Radioactive Substances Scattered by Nuclear Accident / Nowe Technologie Dekontaminacji Radioaktywnych Substancji Rozproszonych Przez Awarie Nuklearna

2013 ◽  
Vol 58 (1) ◽  
pp. 283-290 ◽  
Author(s):  
Y. Nishizaki ◽  
H. Miyamae ◽  
S. Ichikawa ◽  
K. Izumiya ◽  
T. Takano ◽  
...  
Keyword(s):  
Radioactive Waste ◽  
New Technologies ◽  
Ferric Hydroxide ◽  
Nuclear Accident ◽  
Radioactive Cesium ◽  
Cesium Ions ◽  
Naoh Solution ◽  
High Concentrations ◽  
Water Rinsing

Our effort for decontamination of radioactive cesium scattered widely by nuclear accident in March 2011 in Fukushima, Japan has been described. Radioactive cesium scattered widely in Japan has been accumulating in arc or plasma molten-solidified ash in waste incinerating facilities up to 90,000 Bq/kg of the radioactive waste. Water rinsing of the ash resulted in dissolution of cesium ions together with high concentrations of potassium and sodium ions. Although potassium inhibits the adsorption of cesium on zeolite, we succeeded to precipitate cesium by in-situ formation of ferric ferrocyanide and iron rust in the radioactive filtrate after rinsing of the radioactive ash with water. Because the regulation of no preservation of any kind of cyanide substances, cesium was separated from the precipitate consisting of cesium-captured ferric ferrocyanide and ferric hydroxide in diluted NaOH solution and subsequent filtration gave rise to the potassium-free radioactive filtrate. Cesium was captured by zeolite from the potassium-free radioactive filtrate. The amount of this final radioactive waste of zeolite was significantly lower than that of the arc-molten-solidified ash.

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