scholarly journals Growth Supplements for Helicobacter pylori

2000 ◽  
Vol 38 (5) ◽  
pp. 1984-1987 ◽  
Author(s):  
Xiuping Jiang ◽  
Michael P. Doyle
Keyword(s):  
Helicobacter Pylori ◽  
Ferrous Sulfate ◽  
Growth Response ◽  
Culture Media ◽  
Horse Serum ◽  
Brain Heart Infusion ◽  
Lag Time ◽  
Inhibitory Effects ◽  
Sodium Pyruvate ◽  
H Pylori

The growth response of Helicobacter pylori in broth was determined in the presence of ferrous sulfate, sodium pyruvate, and mucin (porcine stomach). The addition of either ferrous sulfate and sodium pyruvate or mucin to brain heart infusion broth with 7% horse serum (BHI-HS) enhanced the growth of H. pylori. The best growth of strain NB2-1, which was the slowest growing of 10 H. pylori strains evaluated, occurred in the presence of 0.05% ferrous sulfate and 0.05% sodium pyruvate. The addition of 0.3% mucin to BHI-HS reduced the lag time of H. pylori by 48 h and enhanced the growth. On the basis of the results for 10 H. pylori strains, the combination of ferrous sulfate (0.025%), sodium pyruvate (0.025%), and mucin (0.15%) in BHI-HS counteracted the inhibitory effects of the antibiotics used in culture media for selective growth of H. pylori. Results suggest that these supplements may be useful for enhancement of the growth of H. pylori in enrichment media.

Effect of Environmental and Substrate Factors on Survival and Growth of Helicobacter pylori

1998 ◽  
Vol 61 (8) ◽  
pp. 929-933 ◽  
Author(s):  
XIUPING JIANG ◽  
MICHAEL P. DOYLE
Keyword(s):  
Helicobacter Pylori ◽  
Horse Serum ◽  
Brain Heart Infusion ◽  
Laboratory Culture ◽  
Effect Of Temperature ◽  
Initial Population ◽  
Nacl Concentration ◽  
Survival And Growth ◽  
H Pylori ◽  
Microaerobic Conditions

The effect of temperature (4 to 42°C), NaCl concentration (0.5 to 7.5%), NaNO2 concentration (0 to 400 μg/ml), water activity (aw level of 0.6 to 0.995), pH (3.5 to 7.3) and urea (8 mM) on the survival and growth of Helicobacter pylori in a nutrient-rich laboratory culture medium was investigated. Under microaerobic conditions (5% O2, 10% CO2, and 85% N2), the organism grew well in brain heart infusion broth supplemented with 7% horse serum and antibiotics (BHI-HS-TVA) in a temperature range of 30 to 37°C with agitation. H. pylori (initial population of ca. 5 × 103 CFU/ml) survived for 14 days at 4°C, for 2 days at 25°C, and for less than 1 day at 40 and 42°C. The optimal NaCl concentration for growth of H. pylori was 0.5 to 1.0%; 2.0% NaCl inhibited growth. Up to 400 μg of NaNO2 per ml did not prevent growth. The minimum aw (adjusted with glycerol) and pH (acidified with HCl) for growth of H. pylori was 0.98 and 4.5, respectively. The addition of urea to broth greatly enhanced the growth of H. pylori at both pH 4.5 and 5.5. Although H. pylori did not grow at pH 3.5, the presence of urea in broth enhanced its survival. Considering the apparent fastidious conditions for growth of H. pylori in BHI-HS-TVA broth, H. pylori is unlikely to grow well, if at all, in most foods. The bacterium may, however, survive for extended periods of time in low acid-high moisture environments under refrigerated storage.

scholarly journals Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori

1998 ◽  
Vol 36 (10) ◽  
pp. 3048-3050 ◽  
Author(s):  
L. K. Siu ◽  
W. K. Leung ◽  
A. F. B. Cheng ◽  
J. Y. Sung ◽  
T. K. W. Ling ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Horse Serum ◽  
Brain Heart Infusion ◽  
Selective Medium ◽  
Gastric Biopsy ◽  
Detection Rates ◽  
Transport Medium ◽  
Selective Transport ◽  
Biopsy Specimens ◽  
H Pylori

Since the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to haveH. pylori infection. Total rates of recovery ofH. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.

Optimizing Enrichment Culture Conditions for Detecting Helicobacter pylori in Foods

2002 ◽  
Vol 65 (12) ◽  
pp. 1949-1954 ◽  
Author(s):  
XIUPING JIANG ◽  
MICHAEL P. DOYLE
Keyword(s):  
Helicobacter Pylori ◽  
Enrichment Culture ◽  
Horse Serum ◽  
Bovine Milk ◽  
Brain Heart Infusion ◽  
Ground Beef ◽  
Culture Conditions ◽  
Enrichment Broth ◽  
H Pylori ◽  
Microaerobic Conditions

The survival and growth of Helicobacter pylori under enrichment conditions in fresh, autoclaved and irradiated ground beef were determined. H. pylori grew in autoclaved ground beef at 37°C under microaerobic conditions in brain heart infusion broth with 7% horse serum at pH 7.3 after 3 to 7 days of lag time but did not grow within 7 days in irradiated (10 kGy) ground beef under the same enrichment conditions. Adjustment of the enrichment broth to pH 5.5 enabled the growth (ca. 2 log10 CFU/ml) of H. pylori within 7 days in the presence of irradiated ground beef and the prolific growth (ca. 3 to 4 log10 CFU/ml) of H. pylori within 3 days in the presence of autoclaved beef. H. pylori in fresh ground beef could not be isolated from enrichment media with antibiotics; however, H. pylori ureA could be detected by polymerase chain reaction (PCR) in such enrichment media after 1 to 3 days of incubation at 37°C. The addition of supplements, i.e., 0.3% mucin, 0.05% ferrous sulfate, and 0.05% sodium pyruvate or 0.008 M urea, or the adjustment of the enrichment broth pH to 5.5 or 4.5 enabled the detection of H. pylori ureA in enrichment media incubated for 1, 2, 3, and/or 7 days at 37°C. H. pylori in sterile milk refrigerated at 4°C at an initial level of 106 CFU/ml was inactivated to an undetectable level within 6 days; however, H. pylori was not detected either by a PCR assay or by the plating of enrichment cultures of 120 raw bovine milk samples.

scholarly journals Plasminogen Binding and Activation at the Surface of Helicobacter pylori CCUG 17874

1998 ◽  
Vol 66 (10) ◽  
pp. 4976-4980 ◽  
Author(s):  
Martina Pantzar ◽  
Åsa Ljungh ◽  
Torkel Wadström
Keyword(s):  
Helicobacter Pylori ◽  
Plasminogen Activator ◽  
Culture Media ◽  
Aminocaproic Acid ◽  
Immunoblot Analysis ◽  
Tissue Type ◽  
Tissue Type Plasminogen Activator ◽  
Ulcer Disease ◽  
Type Plasminogen Activator ◽  
H Pylori

ABSTRACT The binding of iodine-labelled plasminogen to Helicobacter pylori CCUG 17874 was characterized. Inhibition of the binding was observed after preincubation of H. pylori cells with nonradiolabelled plasminogen, lysine, or the lysine analogue ɛ-aminocaproic acid. Fragments of plasminogen, kringles 1 to 3, kringle 4, and mini-plasminogen, were also studied as potential inhibitors. Mini-plasminogen caused total inhibition of the plasminogen binding, while the other fragments caused only partial inhibition. These findings suggest that H. pylori binds specifically the fifth kringle structure of the plasminogen molecule. Plasminogen binding to H. pylori seems to be independent of culture media and independent of the presence of the cytotoxin-associated CagA antigen. Immunoblot analysis identified two plasminogen binding proteins of 57 and 42 kDa. Scatchard plot analysis revealed one binding mechanism with a Kd value of 7 × 10−7 M. Conversion of H. pylori cell-bound plasminogen to plasmin in the presence of a tissue-type plasminogen activator was demonstrated by digestion of the chromogenic substrate S-2251. No activation was noted when plasminogen or tissue-type plasminogen activator was incubated with H. pylori cells alone. Formation of H. pylori cell surface-bound plasmin may be important to provide a powerful proteolytic mechanism for gastric tissue penetration in type B gastritis and peptic ulcer disease, since plasmin degrades not only fibrin but also extracellular matrix proteins such as various collagens and fibronectin.

In vitro inhibition ofHelicobacter pyloribyEnterococcus faeciumGM-1

2005 ◽  
Vol 51 (8) ◽  
pp. 629-636 ◽  
Author(s):  
J H Kang ◽  
M S Lee
Keyword(s):  
Helicobacter Pylori ◽  
Inhibitory Activity ◽  
Enterococcus Faecium ◽  
Culture Supernatant ◽  
Proteolytic Enzymes ◽  
Cell Structure ◽  
Inhibitory Effects ◽  
Biochemical Tests ◽  
H Pylori

A strain of Enterococcus faecium that exhibits antibacterial activity against Helicobacter pylori was isolated from the feces of newborn babies. This strain was selected for its ability to inhibit the growth of H. pylori and to withstand harsh environmental conditions, such as acidic pH and high bile concentration. Biochemical tests and 16S rRNA sequencing specific for Enterococcus faecium GM-1 were used to identify the isolated bacterial strain. In vitro studies were used to investigate the inhibitory effects of E. faecium GM-1 on H. pylori. These results showed that the culture supernatant of E. faecium GM-1 significantly decreased the viability and urease activity of H. pylori. This inhibitory activity remained after adjustment of pH of culture supernatant to neutral. However, treatment with proteolytic enzymes reduced the anti-H. pylori activity of GM-1. Therefore, some substance(s) of E. faecium GM-1 other than pH and lactic acid might be associated with this inhibitory activity. Analysis by electron microscopy also demonstrated that the addition of GM-1 destroyed the cell structure of H. pylori. Additional studies suggested that the binding of H. pylori to human colonial cells decreased in the presence of GM-1.Key words: Enterococcus faecium, Helicobacter pylori, inhibition, human fecal strain, proteinaceous substance(s).

scholarly journals Growth response of Aspergillus flavus IMS1103 isolated from poultry feed

2016 ◽  
Vol 2 (2) ◽  
pp. 221-228 ◽  
Author(s):  
Monzur Morshed Ahmed ◽  
Md Fakruddin ◽  
Md Nur Hossain ◽  
Khandaker Rayhan Mahbub ◽  
Abhijit Chowdhury
Keyword(s):  
Growth Rate ◽  
Aspergillus Flavus ◽  
Growth Response ◽  
Culture Media ◽  
Agar Medium ◽  
Lag Time ◽  
Potato Dextrose Agar ◽  
Poultry Feed ◽  
Effect Of Temperature ◽  
Temperature Range

Aspergillus flavus strains were isolated from locally available poultry feeds. Effect of temperature, pH and culture media on growth of Aspergillus flavus was studied. Temperature ranged from 4-42°C (4, 10, 20, 25, 30, 37 and 42°C) was examined. Except for 4°C and 10°C, the isolate was able to grow for the whole temperature range. The growth was maximum at 25°C and was influenced with increasing or decreasing of temperature from 42°C to 20°C.The lag time was strongly influenced by the temperature at lower temperature level than at higher temperature range. Effect of pH on growth of Aspergillus flavus was also examined; from comparison of 3 different pH levels, it is concluded that at most temperatures pH 6.5 showed a higher growth rate and as a consequence required a shorter time to achieve maximum colony diameter. No significant variations in the lag time were observed. A natural poultry feed meal agar medium (FMAM) was developed in the laboratory and growth of A. flavus was compared with other 2 synthetic dehydrated media namely; Czapek’sdox Agar (CDA) and potato dextrose Agar (PDA). Poultry feed meal agar medium showed better growth response than Czapek’sdox agar and potato dextrose agar at all conditions. At 25°C and pH 6.5 found optimum for growth of Aspergillus flavus in feed meal agar medium whereas, temperature 30°C and pH 6.5 found optimum for growth for Czapek’sdox agar media and temperature 30°C and pH 6 showed high growth rate on potato dextrose agar. Poultry feed meal media showed high affinity for growth of mycelium and early spore formation than other media examined.Asian J. Med. Biol. Res. June 2016, 2(2): 221-228

scholarly journals Chitosan Inhibits Helicobacter pylori Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 542
Author(s):  
Shun-Hsien Chang ◽  
Pei-Ling Hsieh ◽  
Guo-Jane Tsai
Keyword(s):  
Helicobacter Pylori ◽  
Culture Medium ◽  
Fluorescent Microscopy ◽  
Inhibitory Effect ◽  
Carcinoma Cells ◽  
Inhibitory Effects ◽  
Urease Production ◽  
Decreasing Ph ◽  
H Pylori ◽  
Human Gastric Carcinoma Cells

This study investigated the effects of shrimp chitosan with 95% degree of deacetylation (DD95) in combination with clinical antibiotics on the growth and urease production of Helicobacter pylori. The inhibitory effect of DD95 on the adherence of H. pylori to the human intestinal carcinoma cells (TSGH9201) was also investigated. Five strains of H. pylori, including three standard strains and two strains of clinical isolates were used as the test strains. The inhibitory effects of DD95 on growth and urease production of various strains of H. pylori increased with increasing DD95 concentration and decreasing pH values from pH 6.0 to pH 2.0. Urease activity of H. pylori at pH 2.0 in the presence of 4000 μg/mL of DD95 decreased by 37.86% to 46.53%. In the presence of 50 μg/mL antibiotics of amoxicillin, tetracycline, or metronidazole at pH 6.0 and pH 2.0, H. pylori counts were decreased by 1.51–3.19, and 1.47–2.82 Log CFU/mL, respectively. Following the addition of 4000 μg/mL DD95 into the 50 μg/mL antibiotic-containing culture medium with pH 6.0 and pH 2.0, overall H. pylori counts were strongly decreased by 3.67–7.61 and 6.61–6.70 Log CFU/mL, respectively. Further, DD95 could inhibit the adherence of H. pylori on TSGH 9201 cells, as evidenced by fluorescent microscopy and thus may potentially protect against H. pylori infection.

scholarly journals Helicobacter Pylori UreB Upregulates the Expression of PD-L1 through Myh9 /mTOR Pathway and Inhibits the Activation of CD8+ T Cells

2020 ◽  
Author(s):  
Jian Wu ◽  
Honghao Wang ◽  
Xia Guo ◽  
Qinzhen Cai ◽  
Tian Xiang ◽  
...  
Keyword(s):  
T Cells ◽  
Helicobacter Pylori ◽  
Cd8 T Cells ◽  
Mtor Inhibitor ◽  
Mtor Pathway ◽  
Host Cells ◽  
Regulation Mechanism ◽  
Inhibitory Effects ◽  
Culture Model ◽  
H Pylori

Abstract Objectives: Immune regulation mechanism of how Helicobacter pylori urease disrupting the homeostasis of host cells remains unknown.Methods: We thus detected the effect of Helicobacter pylori UreB on macrophage PD-L1 expression with recombinant protein and defective strains. The influence of UreB induced PD-L1 on CD8+ T cells’ proliferation and perforin and granzyme expression were assessed through co-culture model. Results: Urease subset B (UreB) significantly promoted PD-L1 expression in Bone marrow-derived macrophages (BMDMs) and thus blocked the proliferation and activity of H. pylori-primed CD8+ T cells. Myosin heavy chain 9 (Myh9) works as the receptor for UreB. The interaction between UreB and Myh9 promoted amino acid anabolism, activated mTOR pathway and induced PD-L1 expression in BMDMs. mTOR inhibitor Temsirolimus reversed UreB-induced PD-L1 expression and the inhibitory effects on CD8+ T cells. Conclusion: Our study reveals a hitherto-unknown immunosuppressive mechanism of UreB during H. pylori infection, provides clues for the development of H. pylori vaccine.

scholarly journals Survival of Helicobacter pylori in the wastewater treatment process and the receiving river in Michigan, USA

2016 ◽  
Vol 14 (4) ◽  
pp. 692-698 ◽  
Author(s):  
Xiaohui Bai ◽  
Chuanwu Xi ◽  
Jianfeng Wu
Keyword(s):  
Helicobacter Pylori ◽  
Wastewater Treatment ◽  
Treatment Process ◽  
Culture Media ◽  
Treatment Plant ◽  
Resistant Bacteria ◽  
Wastewater Treatment Process ◽  
Ann Arbor ◽  
Huron River ◽  
H Pylori

Contaminated water may play a key role in the transmission of Helicobacter pylori, resulting in gastrointestinal diseases in humans. The wastewater treatment process is an important barrier to control the transmission of H. pylori. However, the presence and viability of H. pylori in the treatment process is not well known. In this paper, the real colony morphology of H. pylori was confirmed by two types of culture media. The survival of H. pylori through the tertiary wastewater treatment process, especially UV disinfection, and in the receiving Huron River in Ann Arbor, Michigan, was investigated by plates cultivation, regular polymerase chain reaction (PCR) assays and quantitative real-time PCR from DNA. The results demonstrated that H. pylori was not only present, but also viable in all processed wastewater samples in the Ann Arbor wastewater treatment plant (WWTP). H. pylori can be found in a higher concentration in the receiving Huron River. There are many kinds of antibiotic- and UV-resistant bacteria, including H. pylori, in the final effluent of Ann Arbor WWTP.

scholarly journals Helicobacter pylori Activates the Early Growth Response 1 Protein in Gastric Epithelial Cells

2004 ◽  
Vol 72 (6) ◽  
pp. 3549-3560 ◽  
Author(s):  
M. M. M. Abdel-Latif ◽  
H. J. Windle ◽  
K. A. Fitzgerald ◽  
Y. S. Ang ◽  
D. Ní Eidhin ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Growth Response ◽  
Early Growth ◽  
Dependent Manner ◽  
Mobility Shift ◽  
Ags Cells ◽  
Early Growth Response ◽  
H Pylori ◽  
Early Growth Response 1

ABSTRACT The early growth response 1 (Egr-1) transcription factor is rapidly induced by various stimuli and is implicated in the regulation of cell growth, differentiation, and gene expression. The aim of this study was to examine the effect of Helicobacter pylori on the expression of Egr-1 and Egr-1-regulated genes in gastric epithelial AGS cells. Egr-1 expression was assayed by immunoblotting and electrophoretic mobility shift assays using H. pylori-stimulated AGS cells. Transient transfection experiments with promoter-reporter constructs of CD44, ICAM-1, and CD95L were used for expression studies. H. pylori induced the expression of Egr-1 in gastric epithelial cell lines in a dose-dependent manner, with the rapid kinetics that are typical of this class of transcription factors. Immunohistochemical studies of biopsies revealed that Egr-1 expression is more abundant in H. pylori-positive patients than in uninfected individuals. Reporter-promoter transfection studies indicated that Egr-1 binding is required for the H. pylori-induced transcriptional promoter activity of the CD44, ICAM-1, and CD95L (APO-1/Fas) constructs. The blocking of egr-1 with an antisense sequence prevented H. pylori-induced Egr-1 and CD44 protein expression. The MEK1/2 signaling cascade participates in H. pylori-mediated Egr-1 expression, but the p38 pathway does not. The data indicate that H. pylori induces Egr-1 expression in AGS cells in vitro and that the Egr-1 protein is readily detectable in biopsies from H. pylori-positive subjects. These observations suggest that H. pylori-associated Egr-1 expression may play a role, in part, in H. pylori-induced pathology.

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