Evidence for the Existence of the Loop E Motif of Potato Spindle Tuber Viroid In Vivo
Keyword(s):
ABSTRACT RNA motifs comprising nucleotides that interact through non-Watson-Crick base pairing play critical roles in RNA functions, often by serving as the sites for RNA-RNA, RNA-protein, or RNA small ligand interactions. The structures of viral and viroid RNA motifs are studied commonly by in vitro, computational, and mutagenesis approaches. Demonstration of the in vivo existence of a motif will help establish its biological significance and promote mechanistic studies on its functions. By using UV cross-linking and primer extension, we have obtained direct evidence for the in vivo existence of the loop E motif of Potato spindle tuber viroid. We present our findings and discuss their biological implications.
2013 ◽
Vol 2013
◽
pp. 1-15
◽
2003 ◽
Vol 23
(24)
◽
pp. 9061-9072
◽
Keyword(s):
2011 ◽
Vol 15
(03)
◽
pp. 174-180
◽
Keyword(s):
1979 ◽
Vol 150
(5)
◽
pp. 1241-1254
◽
1999 ◽
Vol 147
(6)
◽
pp. 1275-1286
◽
Keyword(s):
2005 ◽
Vol 79
(20)
◽
pp. 12721-12731
◽
Keyword(s):