Palladin Compensates for the Arp2/3 Complex and Supports Actin Structures duringListeriaInfections
ABSTRACTPalladin is an important component of motile actin-rich structures and nucleates branched actin filament arraysin vitro. Here we examine the role of palladin duringListeria monocytogenesinfections in order to tease out novel functions of palladin. We show that palladin is co-opted byL. monocytogenesduring its cellular entry and intracellular motility. Depletion of palladin resulted in shorter and misshapen comet tails, and when actin- or VASP-binding mutants of palladin were overexpressed in cells, comet tails disintegrated or became thinner. Comet tail thinning resulted in parallel actin bundles within the structures. To determine whether palladin could compensate for the Arp2/3 complex, we overexpressed palladin in cells treated with the Arp2/3 inhibitor CK-666. In treated cells, bacterial motility could be initiated and maintained when levels of palladin were increased. To confirm these findings, we utilized a cell line depleted of multiple Arp2/3 complex subunits. Within these cells,L. monocytogenesfailed to generate comet tails. When palladin was overexpressed in this Arp2/3 functionally null cell line, the ability ofL. monocytogenesto generate comet tails was restored. Using purified protein components, we demonstrate thatL. monocytogenesactin clouds and comet tails can be generated (in a cell-free system) by palladin in the absence of the Arp2/3 complex. Collectively, our results demonstrate that palladin can functionally replace the Arp2/3 complex during bacterial actin-based motility.IMPORTANCEStructures containing branched actin filaments require the Arp2/3 complex. One of the most commonly used systems to study intracellular movement generated by Arp2/3-based actin motility exploits actin-rich comet tails made byListeria. Using these infections together with live imaging and cell-free protein reconstitution experiments, we show that another protein, palladin, can be used in place of Arp2/3 to form actin-rich structures. Additionally, we show that palladin is needed for the structural integrity of comet tails as its depletion or mutation of critical regions causes dramatic changes to comet tail organization. These findings are the first to identify a protein that can functionally replace the Arp2/3 complex and have implications for all actin-based structures thought to exclusively use that complex.