His-154 is involved in the linkage of the Saccharomyces cerevisiae L-A double-stranded RNA virus Gag protein to the cap structure of mRNAs and is essential for M1 satellite virus expression.
1994 ◽
Vol 14
(4)
◽
pp. 2664-2674
◽
Keyword(s):
The coat protein (Gag) of the double-stranded RNA virus L-A was previously shown to form a covalent bond with the cap structure of eukaryotic mRNAs. Here, we identify the linkage as a phosphoroimidazole bond between the alpha phosphate of the cap structure and a nitrogen in the Gag protein His-154 imidazole side chain. Mutations of His-154 abrogate the ability of Gag to bind to the cap structure, without affecting cap recognition, in vivo virus particle formation from an L-A cDNA clone, or in vitro specific binding and replication of plus-stranded single-stranded RNA. However, genetic analyses demonstrate that His-154 is essential for M1 satellite virus expression.
1994 ◽
Vol 14
(4)
◽
pp. 2664-2674
Keyword(s):
2005 ◽
Vol 86
(9)
◽
pp. 2595-2603
◽
1995 ◽
Vol 15
(5)
◽
pp. 2763-2771
◽
Keyword(s):
2019 ◽
Vol 19
(12)
◽
pp. 950-960
Keyword(s):
Keyword(s):
Keyword(s):