A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.
1987 ◽
Vol 7
(9)
◽
pp. 3268-3276
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Keyword(s):
The poly(A)-binding protein (PAB) gene of Saccharomyces cerevisiae is essential for cell growth. A 66-amino acid polypeptide containing half of a repeated N-terminal domain can replace the entire protein in vivo. Neither an octapeptide sequence conserved among eucaryotic RNA-binding proteins nor the C-terminal domain of PAB is required for function in vivo. A single N-terminal domain is nearly identical to the entire protein in the number of high-affinity sites for poly(A) binding in vitro (one site with an association constant of approximately 2 X 10(7) M-1) and in the size of the binding site (12 A residues). Multiple N-terminal domains afford a mechanism of PAB transfer between poly(A) strands.
1987 ◽
Vol 7
(9)
◽
pp. 3268-3276
◽
1997 ◽
Vol 17
(6)
◽
pp. 3194-3201
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Keyword(s):
1997 ◽
Vol 17
(11)
◽
pp. 6402-6409
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2021 ◽
2014 ◽
Vol 306
(12)
◽
pp. C1167-C1175
◽
1994 ◽
Vol 14
(5)
◽
pp. 3197-3207
2003 ◽
Vol 23
(19)
◽
pp. 7055-7067
◽
1993 ◽
Vol 13
(10)
◽
pp. 6102-6113