scholarly journals Complete Genome Sequences of Pseudomonas alkylphenolica Neo and Variovorax sp. Strain CSUSB, Obtained in Undergraduate Microbiology Courses Using a Hybrid Assembly Approach

2020 ◽  
Vol 9 (9) ◽  
Author(s):  
Christopher Ne Ville ◽  
Dylan Enright ◽  
Ivan Hernandez ◽  
Jeremey Dodsworth ◽  
Paul Orwin
Keyword(s):  
Complete Genome ◽  
Hybrid Approach ◽  
Gram Negative Bacteria ◽  
Hybrid Assembly ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type ◽  
Pesticide Tolerance

Two Gram-negative bacteria with a high G+C content were isolated from soil in undergraduate microbiology classes by enriching for low nutrient growth and neonicotinoid pesticide tolerance. DNA from these isolates was purified and sequenced using a hybrid approach. Here we report the genome sequences of Pseudomonas alkylphenolica strain Neo and Variovorax sp. strain CSUSB.

scholarly journals Complete Genome Sequence of SMBL-WEM22, a Halotolerant Strain of Kosakonia cowanii Isolated from Hong Kong Seawater

2021 ◽  
Vol 10 (41) ◽  
Author(s):  
W. E. Moore ◽  
G. K. K. Lai ◽  
S. D. J. Griffin ◽  
F. C. C. Leung
Keyword(s):  
Hong Kong ◽  
Dna Sequences ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Hybrid Assembly ◽  
Gram Negative ◽  
Content Type ◽  
Facultative Anaerobic ◽  
Long Read

Kosakonia cowanii is a Gram-negative, motile, facultative anaerobic enterobacterium that is found in soil, water, and sewage. K. cowanii SMBL-WEM22 is a halotolerant strain that was isolated from seawater in Hong Kong. The complete genome of SMBL-WEM22 (5,037,617 bp, with a GC content of 55.02%) was determined by hybrid assembly of short- and long-read DNA sequences.

scholarly journals Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Håkon Kaspersen ◽  
Thomas H. A. Haverkamp ◽  
Hanna Karin Ilag ◽  
Øivind Øines ◽  
Camilla Sekse ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Complete Genome ◽  
Flow Cell ◽  
Hybrid Assembly ◽  
Genome Sequences ◽  
Content Type ◽  
Short Reads ◽  
Long Reads ◽  
Long Read ◽  
Hybrid Assemblies

ABSTRACT In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.

scholarly journals Complete Genome Sequences for Pseudomonas sp. Strains 29A and 43A

2020 ◽  
Vol 9 (39) ◽  
Author(s):  
David A. Baltrus ◽  
Meara Clark ◽  
Parris T. Humphrey ◽  
Noah K. Whiteman
Keyword(s):  
Complete Genome ◽  
Hybrid Approach ◽  
Flow Cell ◽  
Pseudomonas Sp ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Cardamine Cordifolia

ABSTRACT Pseudomonas sp. strains 29A and 43A were originally isolated from the phyllosphere of individual plants of Cardamine cordifolia (Brassicaceae). Here, we report complete genome sequences for these two closely related strains, assembled using a hybrid approach combining Illumina paired-end reads and longer reads sequenced on an Oxford Nanopore MinION flow cell.

scholarly journals Complete Genome Sequences of Clinical Pandoraea fibrosis Isolates

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Miranda E. Pitt ◽  
Son H. Nguyen ◽  
Tânia P. S. Duarte ◽  
Louise F. Roddam ◽  
Mark A. T. Blaskovich ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Cystic Fibrosis Patient ◽  
Antibiotic Treatment ◽  
Complete Genome ◽  
Bacterial Species ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type

Pandoraea fibrosis is a newly identified Gram-negative bacterial species that was isolated from the respiratory tract of an Australian cystic fibrosis patient. The complete assembled genome sequences of two consecutive isolates (second isolate collected 11 months after antibiotic treatment) from the same individual are presented here.

scholarly journals Complete Genome Sequence of Lactobacillus plantarum subsp. plantarum Strain LB1-2, Isolated from the Hindgut of European Honeybees, Apis mellifera L., from the Philippines

2018 ◽  
Vol 6 (14) ◽  
Author(s):  
M. Fatima C. Ilagan-Cruzada ◽  
Albert Remus R. Rosana ◽  
Andrew D. Montecillo ◽  
Noel G. Sabino ◽  
Ida F. Dalmacio
Keyword(s):  
Apis Mellifera ◽  
Lactobacillus Plantarum ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gene Clusters ◽  
The Philippines ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type

ABSTRACT Lactobacillus plantarum subsp. plantarum strain LB1-2, isolated from the hindgut of European honeybees in the Philippines, is active against Paenibacillus larvae and has broad activity against several Gram-positive and Gram-negative bacteria. The complete genome sequence reported herein contains gene clusters for multiple bacteriocins and extensive gene inventories for carbohydrate metabolism.

scholarly journals Complete Genome Sequence of Streptomyces sp. Strain BSE7F, a Bali Mangrove Sediment Actinobacterium with Antimicrobial Activities

2018 ◽  
Vol 6 (26) ◽  
Author(s):  
Ira Handayani ◽  
Shanti Ratnakomala ◽  
Puspita Lisdiyanti ◽  
Fahrurrozi ◽  
Mohammad Alanjary ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Antimicrobial Activities ◽  
Mangrove Sediment ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Streptomyces Sp ◽  
Gram Positive Bacteria

The strain Streptomyces sp. BSE7F, a novel Streptomyces strain isolated from Indonesian mangrove sediment, displays antimicrobial activities against Gram-positive bacteria, Gram-negative bacteria, and yeast.

scholarly journals Complete Genome Sequence of the Citrobacter freundii Type Strain

2020 ◽  
Vol 9 (19) ◽  
Author(s):  
Kenneth H. Wan ◽  
Soo Park ◽  
Becky M. Hess ◽  
Michael J. Neff ◽  
Benjamin W. Booth ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Gram Negative Bacteria ◽  
Citrobacter Freundii ◽  
Gram Negative ◽  
Content Type ◽  
The Family ◽  
Facultative Anaerobic

Citrobacter freundii is a species of facultative anaerobic Gram-negative bacteria of the family Enterobacteriaceae. The complete genome is composed of a single chromosomal circle of 4,957,773 bp with a G+C content of 52%.

scholarly journals Whole-Genome Sequences of Two Pseudoalteromonas piscicida Strains, DE1-A and DE2-A, with Strong Antibacterial Activity against Vibrio vulnificus

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Gary P. Richards ◽  
David S. Needleman ◽  
Michael A. Watson ◽  
Shawn W. Polson
Keyword(s):  
Antibacterial Activity ◽  
Vibrio Vulnificus ◽  
Proteolytic Enzymes ◽  
Antibacterial Properties ◽  
Whole Genome ◽  
Gram Negative Bacteria ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type ◽  
Bactericidal Properties

Highly vesiculated Pseudoalteromonas piscicida strains DE1-A and DE2-A were isolated from seawater and show bactericidal properties toward Vibrio vulnificus and other Gram-positive and Gram-negative bacteria. Here, we report the complete genome sequences of these two P. piscicida strains and identify proteolytic enzymes potentially involved in their antibacterial properties.

scholarly journals Complete Genome Sequences of Bordetella pertussis Clinical Isolate FR5810 and Reference Strain Tohama from Combined Oxford Nanopore and Illumina Sequencing

2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Valérie Bouchez ◽  
Sarah Louise Baines ◽  
Sophie Guillot ◽  
Sylvain Brisse
Keyword(s):  
Clinical Isolate ◽  
Bordetella Pertussis ◽  
Complete Genome ◽  
Reference Strain ◽  
Sequence Data ◽  
Hybrid Approach ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

Here, we describe the complete genome sequences of two Bordetella pertussis strains, FR5810, a clinical isolate recovered from the respiratory tract of an infant, and Tohama, a key reference strain for the species. Sequences were obtained using a hybrid approach combining Oxford Nanopore Technologies MinION and Illumina NextSeq 500 sequence data.

scholarly journals Complete Genome Sequence of a Colistin-Resistant Klebsiella pneumoniae Isolate from Houseflies (Musca domestica) in a Trash Disposal Truck in the United States

2020 ◽  
Vol 9 (22) ◽  
Author(s):  
Anwar Kalalah ◽  
Anil Poudel ◽  
Jiansen Gong ◽  
Li Chen ◽  
Yi Yang ◽  
...  
Keyword(s):  
United States ◽  
Klebsiella Pneumoniae ◽  
Genome Sequencing ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type

ABSTRACT A Klebsiella pneumoniae strain isolated from houseflies in a trash disposal truck in the United States was resistant to colistin, a last-resort drug for treating infections caused by multidrug-resistant Gram-negative bacteria. Complete genome sequencing resulted in a total genome size of 5,337,408 bp for this isolate with a plasmid of 224,442 bp.

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