Evaluation of the biosafety of cyanoacrylate adhesive in vitro

2017 ◽  
Vol 10 (4) ◽  
pp. 357-360
Author(s):  
M. S. Belova ◽  
D. G. Korovina ◽  
V. N. Tsygankov ◽  
A. B. Varava ◽  
I. P. Savchenkova ◽  
...  
Keyword(s):  
Cyanoacrylate Adhesive

In Vitro Evaluation of a Cyanoacrylate Bonding Agent

1989 ◽  
Vol 16 (2) ◽  
pp. 75-78 ◽  
Author(s):  
David J. Howells ◽  
Peter Jones
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Composite Resin ◽  
Clinical Use ◽  
Bonding Agents ◽  
In Vitro Evaluation ◽  
Strength Performance ◽  
Cyanoacrylate Adhesive ◽  
Acid Etch

A new cyanoacrylate adhesive developed for orthodontic use was subjected to a controlled in vitro trial of ultimate shear bond strength. Performance deteriorated on storage in saline, rendering the material unsuitable for clinical use. Other alternatives to conventional acid-etch retained composite resin bonding agents are discussed.

scholarly journals Hysteroscopy and the butyl-cyanoacrylate on experimental sterilization of rabbit uterine tubes

2007 ◽  
Vol 22 (5) ◽  
pp. 396-400 ◽  
Author(s):  
Henri Chaplin Rivoire ◽  
Djalma José Fagundes ◽  
Sergio Bigolin ◽  
Anna Tereza Negrini Fagundes
Keyword(s):  
Inflammatory Process ◽  
Sham Group ◽  
Significant Degree ◽  
P Value ◽  
Uterine Tube ◽  
Tube Obstruction ◽  
Cyanoacrylate Adhesive ◽  
Air Insufflation ◽  
Thickness Measurements

PURPOSE: To assess the sterilization effectiveness on uterine tube of rabbit by the cyanoacrylate adhesive. METHODS: Hysteroscopy tubal catheterization was performed randomly in 12 animals (24 uterine tubes) assigned to the sham group (GS) and 15 animals (30 uterine tubes) to the n-butyl-cyanoacrylate (GB). The female rabbits were observed during 30, 90 and 180 days and mated to fertile males. The no pregnant rabbits were submitted to in vitro burst pressure test for patency by air insufflation (40 mmHg). The microscopic assessment was performed to parameters of damages in epithelium caused by the adhesive, the degree of inflammatory process, morphometry data values of tube diameter (UT) (cm), mucosa thickness (MT) and the myosalpinx thickness (MyT) (mm). The mucosa cells densitometry (total optical density) was expressed by the amount of DNA. The significance of the differences in histological scores and in thickness measurements were made by ANOVA test (P value < 0.05). RESULTS: In all animals of GB: the adhesive was attached to the mucosa; there was no pregnancy; no records of significant degree on inflammatory process; the patency test was negative and densitometry of DNA showed similar values to the both groups independently of observation periods. The layers thickness of GB-UT(1.118±0.117), GB-MT(0.447±0.247) and GB-MyT(0.853±0.097) were larger than the GS-UT(0.666±0.409), GS-MT(0.211±0.070) and GS-MyT(0.442±0.143). CONCLUSION: This approach offers a safe and feasible method of uterine tube obstruction.

Bond strength of orthodontic adhesives to dry and saliva-moistened enamel – a comparative in vitro study

2019 ◽  
Vol 2 (96) ◽  
pp. 79-84
Author(s):  
T. Stefański ◽  
A. Kloc-Ptaszna ◽  
L. Postek-Stefańska
Keyword(s):  
Composite Materials ◽  
Bond Strength ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Clinical Situation ◽  
Cyanoacrylate Adhesive ◽  
Significant Difference ◽  
Materials Research ◽  
Mandibular Third Molars

salivamoistened enamel. Design/methodology/approach: One hundred twenty stainless steel brackets were bonded to human mandibular third molars with six materials: Enlight LC® (Ormco Corp), Grengloo® (Ormco Corp), Light Bond® (Reliance Orthodontic Products), Charisma® (Heraeus Kulzer), SmartBond® (Gestenco), and Transbond XT® with MIP® primer (3M Unitek). One half of the specimens in each group (n=10) was bonded to dry enamel, while the other half to saliva-moistened enamel. Bond strength testing was performed with a wire loop loading technique after 30-min incubation in artificial saliva. Failure mode was evaluated using adhesive remaining index (ARI). Findings: No significant differences in bond strength to dry enamel were noted for all composite materials (p>0.05). SmartBond exhibited significantly greater bond strength to moistened enamel (7.10 ± 1.47 MPa) and comparable with other composite materials to dry enamel. Composite materials demonstrated significantly reduced bond strength to saliva-moistened enamel (p<0.001), except for Transbond MIP, whose bond strength was not significantly decreased by saliva contamination (p=0.089). There was not statistically significant difference between bond strength of SmartBond and Transbond to saliva contaminated enamel. A higher incidence of cohesive failures was noted for all materials. Research limitations/implications: Composite light-cured materials provide adequate bond strength to dry enamel. Cyanoacrylate adhesive provides sufficient bond strength only under moist conditions. Transbond XT+MIP has sufficient bond strength to either dry or saliva-moistened enamel. Practical implications: The study evaluated the bonding strength of orthodontic brackets, which is a critical parameter in orthodontics, especially in situations that do not allow for proper isolation from saliva, such as bonding to impacted teeth following surgical exposure. Originality/value: Bond strengths evaluated with loop loading technique in different study environments would help the orthodontist to choose the most effective adhesive for the specific clinical situation.

scholarly journals Analysis of stress in the walls of simulated artificial root canals during instrumentation with Reciproc system: a pilot study using a photoelastic model

2016 ◽  
Vol 19 (3) ◽  
pp. 88
Author(s):  
Ana Elisa Vilela ◽  
Denise Pontes Raldi ◽  
Flavia Goulart Rosa Cardoso ◽  
Cristiane Aparecida de Assis Claro ◽  
Sandra Márcia Habitante
Keyword(s):  
Pilot Study ◽  
Video Camera ◽  
Distal Region ◽  
Dark Field ◽  
Field Configuration ◽  
Root Canals ◽  
System A ◽  
Cyanoacrylate Adhesive ◽  
Working Length

<p><strong>Objective:</strong> The aim of this pilot study was to perform <em>in vitro</em> analysis of the stress related to instrumentation of artificial root canals with Reciproc System by using the photoelasticity method. <strong>Material and Methods:</strong> Photoelastic models consisted of two epoxy resin blocks simulating root canals, which were attached with cyanoacrylate adhesive to a base and placed at the centre of a circular polariscope in a dark-field configuration. The Reciproc R25 instrument was mounted to a VDW motor and used in block 1 up to 12 mm (working length) and then the same instrument was used in block 2. The images were captured by video camera and analysed at the time of the fourth penetration. Isochromatic fringes were observed in the cervical, middle and apical thirds at mesial and distal regions of each block. Therefore, they were divided into cervical-mesial (CM), cervical-distal (CD), middle-mesial (MM), middle-distal (MD), apical-mesial (AM) and apical-distal (AD). <strong>Results:</strong> In the first instrumentation, it was found that the greatest stress occurred at the middle-distal region (1.38), followed by middle-mesial (1.20), apical-distal (1.20) and apical-mesial regions (1.20). In the second instrumentation, the greatest stress occurred at the middle-mesial (1.20), apical-distal (1.20), apical-mesial (1.20) and middle-distal regions (0.90). <strong>Conclusion:</strong> The greatest stress occurred in the middle and apical thirds during the first instrumentation. Re-utilization caused less stress.</p><p> </p><p><strong>Keywords</strong></p><p>Dental Stress Analysis; Endodontic; Instrumentation.</p><p> </p>

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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