Covid-19: MHRA is concerned over use of rapid lateral flow devices for mass testing

BMJ ◽  
2021 ◽  
pp. n1090
Author(s):  
Gareth Iacobucci
Keyword(s):  
Lateral Flow ◽  
Flow Devices

Lateral flow devices for nucleic acid analysis exploiting quantum dots as reporters

2015 ◽  
Vol 864 ◽  
pp. 48-54 ◽  
Author(s):  
Eleni A. Sapountzi ◽  
Sotirios S. Tragoulias ◽  
Despina P. Kalogianni ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos
Keyword(s):  
Quantum Dots ◽  
Nucleic Acid ◽  
Lateral Flow ◽  
Nucleic Acid Analysis ◽  
Flow Devices

scholarly journals Validation of Two Commercial Lateral Flow Devices for the Detection of Peanut Proteins in Cookies: Interlaboratory Study

2006 ◽  
Vol 89 (2) ◽  
pp. 462-468 ◽  
Author(s):  
Arjon J van Hengel ◽  
Claudia Capelletti ◽  
Marcel Brohee ◽  
Elke Anklam ◽  
M-C S Baumgartner ◽  
...  
Keyword(s):  
False Negative ◽  
Lateral Flow ◽  
Food Matrix ◽  
Negative Results ◽  
False Negative Results ◽  
Test Kit ◽  
Interlaboratory Validation ◽  
Peanut Proteins ◽  
Flow Devices ◽  
Positive Results

Abstract Results are reported for an interlaboratory validation study of 2 commercially available lateral flow devices (dipstick tests) designed to detect peanut residues in food matrixes. The test samples used in this study were cookies containing peanuts at 7 different concentrations in the range of 030 mg peanuts/kg food matrix. The test samples with sufficient and proven homogeneity were prepared in our laboratory. The analyses of the samples (5 times per level by each laboratory) were performed by 18 laboratories worldwide, which submitted a total of 1260 analytical results. One laboratory was found to be an outlier for one of the test kits. In general, both test kits performed well. However, some false-negative results were reported for all matrixes containing <21 mg peanuts/kg cookie. It must be stressed that the test kits were challenged beyond their cut-off limits (5 mg/kg, depending on the food matrix). One test kit showed fewer false-negative results, but it led to some false-positive results for the blank materials. The sensitivity of the dipstick tests approaches that achieved with enzyme-linked immunosorbent assays.

Evaluation of rapid test kits for quantification of deoxynivalenol in naturally contaminated oats and wheat

2012 ◽  
Vol 5 (4) ◽  
pp. 339-350 ◽  
Author(s):  
H.U. Aamot ◽  
I.S. Hofgaard ◽  
G. Brodal ◽  
O. Elen ◽  
M. Jestoi ◽  
...  
Keyword(s):  
European Union ◽  
Rapid Test ◽  
Reference Method ◽  
Lateral Flow ◽  
First Report ◽  
Ms Analysis ◽  
Simultaneous Testing ◽  
Immunosorbent Assays ◽  
Flow Devices

The aim of this study was to evaluate the performance of rapid test kits for analysis of deoxynivalenol (DON), a mycotoxin commonly found in small-grain cereals. Concentrations of DON and its acetylated derivative 3-acetyldeoxynivalenol (3-ADON) were analysed in naturally contaminated wheat (n=56) and oats (n=81) using two enzyme-linked immunosorbent assays, i.e. Ridascreen® DON (‘Standard ELISA’) and Ridascreen® FAST DON (‘Fast ELISA’), and two lateral flow devices, i.e. Rida® QUICK DON (‘Quick LFD’) and Rosa® DON (‘Rosa LFD’). Analysis by the LC-MS/MS reference method showed a median DON + 3-ADON concentration of 1,168 μg/kg (90th percentile 3,264 μg/kg) in wheat and 2,250 μg/kg (90th percentile of 9,008 μg/kg) in oats. Rosa LFD offered the best reliability, achieving detection that was stable across toxin levels in both matrices. In addition, Rosa LFD had the highest accuracy (i.e. proportion of correct results) at a DON level of 1,250 μg/kg in wheat (0.83) and 8,000 μg/kg in oats (0.96). Standard ELISA achieved the highest accuracy at a DON level of 1,750 μg/kg in oats (0.85). Detection by Quick LFD in wheat and Fast ELISA in both wheat and oats differed significantly among toxin levels (P≤0.05), with recoveries outside European Union requirements (70-120%) in some cases. The correlation with results from LC-MS/MS analysis in oats was best for Rosa LFD (R2=0.79). In wheat, however the correlation was best for Fast ELISA (R2=0.81). Rosa LFD was regarded as well-suited for on-site detection. Standard ELISA allowed simultaneous testing of several samples and achieved detection that was stable across toxin levels in both matrices, making it a useful alternative in laboratories. To our knowledge, this is the first report describing the performance of rapid test kits for detection of DON in naturally contaminated oats.

scholarly journals Improved sensitivity and limit-of-detection of lateral flow devices using spatial constrictions of the flow-path

2018 ◽  
Vol 113 ◽  
pp. 95-100 ◽  
Author(s):  
Ioannis N. Katis ◽  
Peijun J.W. He ◽  
Robert W. Eason ◽  
Collin L. Sones
Keyword(s):  
Limit Of Detection ◽  
Flow Path ◽  
Lateral Flow ◽  
Flow Devices

Synthesis and characterization of colloidal gold particles as labels for antibodies as used in lateral flow devices

The Analyst ◽  
2012 ◽  
Vol 137 (8) ◽  
pp. 1882 ◽  
Author(s):  
Barbara Cvak ◽  
Dietmar Pum ◽  
Alexandra Molinelli ◽  
Rudolf Krska
Keyword(s):  
Colloidal Gold ◽  
Lateral Flow ◽  
Synthesis And Characterization ◽  
Gold Particles ◽  
Flow Devices

Feasibility of Immunodiagnostic Devices for the Detection of Ricin, Amanitin, and T-2 Toxin in Food

2005 ◽  
Vol 68 (6) ◽  
pp. 1294-1301 ◽  
Author(s):  
ERIC A. E. GARBER ◽  
ROBERT M. EPPLEY ◽  
MICHAEL E. STACK ◽  
MICHAEL A. McLAUGHLIN ◽  
DOUGLAS L. PARK
Keyword(s):  
High Throughput Screening ◽  
Limit Of Detection ◽  
Extraction Methods ◽  
Food Samples ◽  
Lateral Flow ◽  
Health Concern ◽  
High Background ◽  
Baked Goods ◽  
Simple Extraction ◽  
Flow Devices

Qualitative and quantitative comparisons were conducted of commercially available immunodiagnostic devices for the detection of three select agents with oral LD50 values ≥0.1 mg/kg of body weight. Ricin (oral LD50 > 1 mg/kg), amanitin (oral LD50 approximately 0.1 mg/kg), and T-2 toxin (oral LD50 > 1 mg/kg) were spiked into beverages, produce, dairy, and baked goods and assayed using commercially available enzyme-linked immunosorbent assays (ELISAs) and lateral flow devices. In all cases, the commercial diagnostic kits successfully detected all three select agents at concentrations below what might be a health concern. The considerable difference between the limit of detection of the immunodiagnostic devices employed (typically ≤0.020 μg/g) and the amount of the select agent necessary to pose a health threat in a single serving of food facilitated the design of protocols for the high throughput screening of food samples. These protocols entailed simple extraction methods followed by sample dilution. Lateral flow devices and sandwich ELISAs for the detection of ricin had no significant background problems due to the food matrices. Competitive ELISAs, which typically have unacceptably high background reactions with food samples, successfully detected amanitin and T-2 toxin.

scholarly journals Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 97 ◽  
Author(s):  
Peijun He ◽  
Ioannis Katis ◽  
Robert Eason ◽  
Collin Sones
Keyword(s):  
Bacterial Infections ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Direct Write ◽  
Multiplexed Detection ◽  
Amyloid A ◽  
Flow Devices ◽  
Laser Direct Write

Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure.

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