scholarly journals P623 Post-transcriptional regulation of genes by non-coding RNA inneisseria gonorrhoeae, an obligate human pathogen

2019 ◽  
Author(s):  
Pooja Tanwer ◽  
Daman Saluja
Keyword(s):  
Transcriptional Regulation ◽  
Human Pathogen ◽  
Non Coding Rna ◽  
Post Transcriptional Regulation

scholarly journals Thioredoxin post-transcriptional regulation by H19 provides a new function to mRNA-like non-coding RNA

Oncogene ◽  
2002 ◽  
Vol 21 (10) ◽  
pp. 1625-1631 ◽  
Author(s):  
Séverine Lottin ◽  
Anne-Sophie Vercoutter-Edouart ◽  
Eric Adriaenssens ◽  
Xavier Czeszak ◽  
Jérôme Lemoine ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Non Coding Rna ◽  
Post Transcriptional Regulation

scholarly journals Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA

2007 ◽  
Vol 35 (3) ◽  
pp. 999-1006 ◽  
Author(s):  
Véronique Arluison ◽  
Sungchul Hohng ◽  
Rahul Roy ◽  
Olivier Pellegrini ◽  
Philippe Régnier ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Spectroscopic Observation ◽  
Rna Chaperone ◽  
Non Coding Rna ◽  
Post Transcriptional Regulation

scholarly journals Novel Aspects of Nitrate Regulation in Arabidopsis

2020 ◽  
Vol 11 ◽  
Author(s):  
Hongmei Fan ◽  
Shuxuan Quan ◽  
Shengdong Qi ◽  
Na Xu ◽  
Yong Wang
Keyword(s):  
Transcriptional Regulation ◽  
Regulatory Network ◽  
Growth And Development ◽  
Significant Progress ◽  
Plant Growth And Development ◽  
General Picture ◽  
The Past ◽  
Non Coding Rna ◽  
Post Transcriptional Regulation ◽  
Made In

Nitrogen (N) is one of the most essential macronutrients for plant growth and development. Nitrate (NO3–), the major form of N that plants uptake from the soil, acts as an important signaling molecule in addition to its nutritional function. Over the past decade, significant progress has been made in identifying new components involved in NO3– regulation and starting to unravel the NO3– regulatory network. Great reviews have been made recently by scientists on the key regulators in NO3– signaling, NO3– effects on plant development, and its crosstalk with phosphorus (P), potassium (K), hormones, and calcium signaling. However, several novel aspects of NO3– regulation have not been previously reviewed in detail. Here, we mainly focused on the recent advances of post-transcriptional regulation and non-coding RNA (ncRNAs) in NO3– signaling, and NO3– regulation on leaf senescence and the circadian clock. It will help us to extend the general picture of NO3– regulation and provide a basis for further exploration of NO3– regulatory network.

scholarly journals Post-transcriptional regulation of microRNAs in cancer: From prediction to validation

2018 ◽  
Author(s):  
Sheril June Ankasha ◽  
Mohamad Nasir Shafiee ◽  
Norhazlina Abdul Wahab ◽  
Raja Affendi Raja Ali ◽  
Norfilza Mohd Mokhtar
Keyword(s):  
Transcriptional Regulation ◽  
Messenger Rna ◽  
Vital Role ◽  
Transcriptional Level ◽  
Rna Expression ◽  
Biological Processes ◽  
Target Region ◽  
Non Coding Rna ◽  
Target Sites ◽  
Post Transcriptional Regulation

MicroRNA (miRNA) is a small non-coding RNA with an established function to regulate genes at the post-transcriptional level leading to suppression or degradation of its messenger RNA expression (mRNA). Its dysregulation plays a vital role in a variety of biological and pathological processes including cancer. A lot of algorithms have been established to predict the target sites of miRNA, but experimentally identifying and validating its target region is still lacking. Guidance in experimental procedures is really needed to find genuine miRNA targets. Therefore, in this review, we provide an outline on the workflow in predicting and validating the targeted sites of miRNA using several methods as a guideline for the scientists. The final outcome of this type of experiment is essential to explore the major impact of miRNAmRNA interaction involved in the biological processes and to assist miRNA-based drug development in the future.

scholarly journals The Intergenic Small Non-Coding RNA ittA is Required for Optimal Infectivity and Tissue Tropism in Borrelia burgdorferi

2020 ◽  
Author(s):  
Diana N. Medina-Pérez ◽  
Beau Wager ◽  
Erin Troy ◽  
Lihui Gao ◽  
Steven J. Norris ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Borrelia Burgdorferi ◽  
The United States ◽  
Heart Tissue ◽  
Translational Efficiency ◽  
Rna Molecules ◽  
Regulatory Processes ◽  
Non Coding Rna ◽  
Small Regulatory Rnas ◽  
Post Transcriptional Regulation

ABSTRACTPost-transcriptional regulation via small regulatory RNAs (sRNAs) has been implicated in diverse regulatory processes in bacteria, including virulence. One class of sRNAs, termed trans-acting sRNAs, can affect the stability and/or the translational efficiency of regulated transcripts. In this study, we utilized a collaborative approach that employed data from infection with the Borrelia burgdorferi Tn library, coupled with Tn-seq, together with borrelial sRNA and total RNA transcriptomes, to identify an intergenic trans-acting sRNA, which we designate here as ittA for infectivity-associated and tissue-tropic sRNA locus A. The genetic inactivation of ittA resulted in a significant attenuation in infectivity, with decreased spirochetal load in ear, heart, skin and joint tissues. In addition, the ittA mutant did not disseminate to peripheral skin sites or heart tissue, suggesting a role for ittA in regulating a tissue-tropic response. RNA-Seq analysis determined that 19 transcripts were differentially expressed in the ittA mutant relative to its genetic parent, including vraA, bba66, ospD and oms28 (bba74). Subsequent proteomic analyses also showed a significant decrease of OspD and Oms28 (BBA74) proteins. To our knowledge this is the first documented intergenic sRNA that alters the infectivity potential of B. burgdorferi.AUTHOR SUMMARYLyme disease is a tick-borne infection mediated by the spirochetal bacterium, Borrelia burgdorferi, that is responsible for greater than 300,000 infections in the United States per year. As such, additional knowledge regarding how this pathogen modulates its regulatory armamentarium is needed to understand how B. burgdorferi establishes and maintains infection. The identification and characterization of small, non-coding RNA molecules in living systems, designated as sRNAs, has recalibrated how we view post-transcriptional regulation. Recently, over 1,000 sRNAs were identified in B. burgdorferi. Despite the identification of these sRNAs, we do not understand how they affect infectivity or B. burgdorferi pathogenesis related outcomes. Here, we characterize the ittA B. burgdorferi sRNA and show that it is essential for optimal infection using murine experimental infection as our readout. We also track the effect of this sRNA on the transcriptional and proteomic profile as the first step in providing mechanistic insight into how this important sRNA mediates its regulatory effect.

scholarly journals Synthetic Artificial Long Non-coding RNA Shows Higher Efficiency in Specific Malignant Phenotype Inhibition Compared to the CRISPR/Cas Systems

2020 ◽  
Vol 7 ◽  
Author(s):  
Lin Yao ◽  
Quan Zhang ◽  
Aolin Li ◽  
Binglei Ma ◽  
Zhenan Zhang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Transcriptional Regulation ◽  
Cancer Cell ◽  
Bladder Cancer Cell ◽  
Luciferase Assay ◽  
Therapeutic Effects ◽  
Malignant Phenotype ◽  
Non Coding Rna ◽  
Post Transcriptional Regulation ◽  
Long Non Coding Rna

Objective: Both oncogenic transcription factors (TFs) and microRNAs (miRNAs) play an important regulator in human cancer by transcriptional and post-transcriptional regulation, respectively. These phenomena raise questions about the ability of artificial device to regulate miRNAs and TFs simultaneously. In this study, we aimed to construct an artificial long non-coding RNA, “alncRNA,” which imitated CRISPR/Cas systems and to illuminate its therapeutic effects in bladder cancer cell lines. At the same time, we also compared the efficiency of alncRNA and CRISPR/Cas systems in regulating gene expression.Study Design and Methods: Based on engineering principles of synthetic biology, we combined tandem arrayed cDNA sequences of aptamer for TFs with tandem arrayed cDNA copies of binding sites for the miRNAs to construct alncRNA. In order to prove the utility of this platform, we chose β -catenin, NF-κB, miR-940, and miR-495 as the functional targets and used the bladder cancer cell lines 5637 and T24 as the test models. Real-time Quantitative PCR (qPCR), dual-luciferase assay and relative phenotypic experiments were applied to severally test the expression of relative gene and therapeutic effects of our devices.Result: Dual-luciferase assay indicated alncRNA could inhibit transcriptional activity of TFs. What’s more, the result of qPCR showed that expression levels of the relative TFs target genes and miRNAs were reduced by corresponding alncRNA and the inhibitory effect was better than CRIPSR dCas9-KRAB. By functional experiments, decreased cell proliferation, increased apoptosis, and motility inhibition were observed in alncRNA-infected bladder cells.Conclusion: In summary, our synthetic devices indeed function as anti-tumor regulator, which synchronously accomplish transcriptional and post-transcriptional regulation in bladder cancer cell and show higher efficiency in specific malignant phenotype inhibition compared to the CRISPR/Cas systems. Most importantly, Anti-cancer effects were induced by the synthetic alncRNA in the bladder cancer lines. Our devices, therefore, provides a novel strategy for cancer therapy and could be a useful “weapon” for cancer cell.

43-OR: Post-transcriptional Regulation of Slc16a1 mRNA Is Essential for Maintaining Normal ß-Cell Function

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 43-OR
Author(s):  
DINA MOSTAFA ◽  
AKINORI TAKAHASHI ◽  
TADASHI YAMAMOTO
Keyword(s):  
Transcriptional Regulation ◽  
Cell Function ◽  
Post Transcriptional Regulation
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