Developmental morphology of the flower of Anthurium jenmanii: a new element in our understanding of basal Araceae

Botany ◽  
2008 ◽  
Vol 86 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Denis Barabé ◽  
Christian Lacroix
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Floral Development ◽  
Developmental Morphology ◽  
Calcium Oxalate Crystals ◽  
Stages Of Development ◽  
Early Stages ◽  
Floral Primordia ◽  
Scanning Electron

The early stages of development of the inflorescence of Anthurium jenmanii Engl. were examined using scanning electron microscopy. The inflorescence of A. jenmanii consists of more than 100 flowers arranged in recognizable spirals. Each flower has four broad tepals enclosing four stamens that are not visible prior to anthesis. The gynoecium consists of two carpels. The floral primordia are first initiated on the lower portion of the inflorescence, they then increase in size and appear as transversely extended bulges. The two lateral tepals are the first organs to be initiated, followed shortly thereafter by the two median tepals. The two lateral stamens are initiated first, directly opposite the lateral tepals, and are followed by two median stamens initiated directly opposite the median tepals. A two-lobed stigma is clearly visible during the early stages of development of the gynoecium. On some of the young inflorescences, all floral parts were covered by extracellular calcium oxalate crystals. The release of these prismatic crystals occurs before the stamens and petals have reached maturity. The mode of floral development observed in Anthurium has similarities with that reported for Gymnostachys . However, contrary to Gymnostachys, the development of the flower of A. jenmanii is not unidirectional.

Identification of calcium oxalate crystals in dried or fixed tobacco leaf

1984 ◽  
Vol 42 ◽  
pp. 288-289
Author(s):  
Vicki L. Baliga ◽  
Mary Ellen Counts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Growth And Development ◽  
Polarized Light ◽  
Calcium Oxalate Crystals ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Calcium is an important element in the growth and development of plants and one form of calcium is calcium oxalate. Calcium oxalate has been found in leaf seed, stem material plant tissue culture, fungi and lichen using one or more of the following methods—polarized light microscopy (PLM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction.Two methods are presented here for qualitatively estimating calcium oxalate in dried or fixed tobacco (Nicotiana) leaf from different stalk positions using PLM. SEM, coupled with energy dispersive x-ray spectrometry (EDS), and powder x-ray diffraction were used to verify that the crystals observed in the dried leaf with PLM were calcium oxalate.

Effect of Seed Crystals of Uric Acid and Monosodium Urate on the Crystallization of Calcium Oxalate in Undiluted Human Urine in Vitro

1997 ◽  
Vol 92 (2) ◽  
pp. 205-213 ◽  
Author(s):  
Phulwinder K. Grover ◽  
Rosemary L. Ryall
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Uric Acid ◽  
Calcium Oxalate ◽  
Human Urine ◽  
Monosodium Urate ◽  
Calcium Oxalate Crystals ◽  
Seed Crystals ◽  
Oxalate Crystals ◽  
Scanning Electron

1. The aim of this study was to determine whether seed crystals of uric acid or monosodium urate promote the epitaxial deposition of calcium oxalate in undiluted human urine. The effects of seed crystals of uric acid, monosodium urate or calcium oxalate on calcium oxalate crystallization induced in pooled 24-h urine samples collected from six healthy men were determined by [14C]oxalate deposition and Coulter counter particle analysis. The precipitated crystals were examined by scanning electron microscopy. 2. Seed crystals of uric acid, monosodium urate and calcium oxalate increased the precipitated particle volume in comparison with the control containing no seeds by 13.6%, 56.8% and 206.5% respectively, whereas the deposition of [14C]oxalate in these samples relative to the control was 1.4% (P < 0.05), 5.2% (P < 0.01) and 54% (P < 0.001) respectively. The crystalline particles deposited in the presence of monosodium urate seeds were smaller than those in the control samples. Scanning electron microscopy showed that large aggregates of calcium oxalate were formed in the presence of calcium oxalate seeds, which themselves were not visible. In contrast, monosodium urate and, to a lesser extent, uric acid seeds were scattered free on the membrane surfaces and attached like barnacles upon the surface of the calcium oxalate crystals. 3. It was concluded that seed crystals of monosodium urate and uric acid do not promote calcium oxalate deposition to a physiologically significant degree in urine. Howsever, binding of monosodium urate and uric acid crystals and their subsequent enclosure within actively growing calcium oxalate crystals might occur in vivo, thereby explaining the occurrence of mixed urate/oxalate stones.

scholarly journals Concanavalin a and wheat germ agglutinin receptors on dictyostelium: Their visualization by scanning electron microscopy with microspheres

1976 ◽  
Vol 71 (1) ◽  
pp. 314-322 ◽  
Author(s):  
R Molday ◽  
R Jaffe ◽  
D McMahon
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Surface ◽  
Wheat Germ ◽  
Cellular Interactions ◽  
Con A ◽  
Stages Of Development ◽  
Lectin Receptors ◽  
Plant Lectins ◽  
Scanning Electron

The cellular slime mold, Dictyostelium discoideum, is a convenient model for studying cellular interactions during development. Evidence that specific cell surface components are involved in cellular interactions during its development has been obtained by Gerisch and co-workers (1, 2) using immunological techniques. Smart and Hynes (3) have shown that a cell surface protein can be iodinated on cells in aggregation phase, but not in vegetative phase, by the lactoperoxidase procedure. Recently, McMahon et al. (4), and Hoffman and McMahon have demonstrated, by SDS gel electrophoresis, considerable differences in cell surface proteins and glycoproteins of plasma membranes isolated from cells at different stages of development. Plant lectins have also been used to monitor changes in cell surface properties of D. discoideum cells during development. Weeks and co-workers (5, 6) have detected differences in the binding and agglutination of cells by concanavalin A (Con A). Gillette and Filosa (7) have shown that Con A inhibits cell aggregation and prematurely induces cyclic AMP phosphodiesterase. Capping of Con A receptors has also been reported (8). Reitherman et al. (9) have recently reported that agglutination of cells by several plant lectins and the slime mold agglutination, discoidin, changes during development. Such studies indicate that differences in surface properties exist for cells at various stages of development. However, owing to the uncertainties in the factors which contribute to lectin-induced cell agglutination (10), the molecular basis for these observations remain to be determined. In this study, we have used microspheres (11-14) coupled to either Con A or wheat germ agglutinin (WGA) as visual markers to study by scanning electron microscopy the topographical distribution of lectin receptors on D. discoideum cells fixed at different stages of development. We also describe the effect of labeling on the distribution of lectin receptors and on the morphology of the cell surface.

Investigation of flowering in Banksia baxteri R. Br. and B. hookeriana Meissner for improving pruning practices

1994 ◽  
Vol 34 (8) ◽  
pp. 1209 ◽  
Author(s):  
LJ Rohl ◽  
AM Fuss ◽  
JA Dhaliwal ◽  
MG Webb ◽  
BB Lamont
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Floral Development ◽  
Early Summer ◽  
Stem Length ◽  
First Year ◽  
Floral Initiation ◽  
Scanning Electron

Floral initiation and development in relation to time of flowering were investigated in Banksia baxteri and B. hookeriana with the aid of scanning electron microscopy. Floral initiation occurred in spring in B. baxteri and in early summer in B. hookeriana. Floral development was rapid in B. baxteri (3 months to reach anthesis in summer). In B. hookeriana, development took 5 months, with anthesis occurring in winter. Most B. hookeriana blooms were produced on 2-year-old shoots, while B. baxteri produced about half of its blooms on 2-year-old shoots and almost as many on 3-year-old shoots. In both species, shoots that flowered within 2 years were longer and thicker in their first year than other shoots. A critical minimum stem length was determined for the first year's growth, to be used as a criterion for determining which shoots to remove during pruning. Details are provided for the timing of pruning to achieve maximum bloom production in B. baxteri and B. hookeriana.

scholarly journals Ovule Structure of Scotch thistle Onopordum acanthium L. (Cynareae, Asteraceae)

2016 ◽  
Vol 58 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Jolanta Kolczyk ◽  
Piotr Stolarczyk ◽  
Bartosz J. Płachno
Keyword(s):  
Electron Microscopy ◽  
Calcium Oxalate ◽  
Calcium Oxalate Crystals ◽  
Polarizing Microscopy ◽  
Onopordum Acanthium ◽  
Oxalate Crystals ◽  
Transmission Electron ◽  
Mature Ovule ◽  
Vacuolated Cells ◽  
Scanning Electron

AbstractStudies concerning the ultrastructure of the periendothelial zone integumentary cells of Asteraceae species are scarce. The aim was to check whether and/or what kinds of integument modifications occur inOnopordum acanthium. Ovule structure was investigated using light microscopy, scanning electron microscopy, transmission electron microscopy and histochemistry. For visualization of calcium oxalate crystals, the polarizing microscopy was used. The periendothelial zone of integument inO. acanthiumis well developed and composed of mucilage cells near the integumentary tapetum and large, highly vacuolated cells at the chalaza and therefore they differ from other integumentary cells. The cells of this zone lack starch and protein bodies. Periendothelial zone cells do not have calcium oxalate crystals, in contrast to other integument cells. The disintegration of periendothelial zone cells was observed in a mature ovule. The general ovule structure ofO. acanthiumis similar to other members of the subfamily Carduoideae, although it is different to “Taraxacum”, “Galinsoga” and “Ratibida” ovule types.

First Evidence for the Presence of Weddellite Crystallites in Opuntia ficus indica Parenchyma

2003 ◽  
Vol 58 (11-12) ◽  
pp. 812-816 ◽  
Author(s):  
Mohamed E. Malainine ◽  
Alain Dufresne ◽  
Danielle Dupeyre ◽  
Michel R. Vignon ◽  
Mostafa Mahrouz
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
X Ray Diffraction ◽  
Experimental Protocol ◽  
Opuntia Ficus Indica ◽  
Hydration Level ◽  
X Ray ◽  
Opuntia Species ◽  
Scanning Electron

Abstract Calcium oxalate crystallites occur very often in the plants tissues and their role is still poorly known. We report here the experimental protocol leading to the isolation of two forms of calcium oxalate crystallites differing in their hydration level in the parenchymal tissues of Opuntia ficus indica (Miller). Whereas the whewellite crystallites are habitual in all Opuntia species, the weddellite form has never been isolated from these species before, which is probably due to their small size (about 1 μm). We have identified these forms using X-ray diffraction and scanning electron microscopy.

scholarly journals Architectural Development of Inflorescence in Hydrangea macrophylla cv. Hermann Dienemann

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 361-365 ◽  
Author(s):  
Gilles Galopin ◽  
Sandrine Codarin ◽  
Jean-Daniel Viemont ◽  
Philippe Morel
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Inflorescence Development ◽  
Schematic Representation ◽  
Early Stages ◽  
Hydrangea Macrophylla ◽  
Floral Differentiation ◽  
Architectural Development ◽  
Scanning Electron

Architectural development of inflorescence in Hydrangea macrophylla cv. Hermann Dienemann was observed using scanning electron microscopy. The study of inflorescence morphogenesis shows that the architecture is of the dichasial type. The first two orders of branching are initiated from a dichasial branching without floral differentiation. The following orders present floral differentiation. They determine the formation of small units through the development of composite dichasium into biparous and uniparous cymes. This research makes it possible to establish a schematic representation of the first phases of inflorescence development and to define early stages of inflorescence morphogenesis.

Sign in / Sign up

Export Citation Format

Share Document