SAFFLOWER LEAF TISSUE CULTURES

1956 ◽  
Vol 34 (6) ◽  
pp. 825-829 ◽  
Author(s):  
Franziska L. M. Turel ◽  
Mary M. Howes
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Leaf Tissue ◽  
Dry Weight ◽  
Coconut Milk ◽  
Tissue Cultures ◽  
Indole 3 Acetic Acid

A tissue culture was obtained from the cells around the vein of a piece of normal safflower leaf. The tissue has now been transferred monthly for two and one-half years. Growth was measured on White's, Knop's, and Heller's media with and without 0.1 mgm. indole-3-acetic acid per liter and/or 10% coconut milk. Indole-3-acetic acid had no effect but coconut milk greatly enhanced growth. Heller's medium plus coconut milk was the best for growth of the leaf tissue. The addition of coconut milk to White's medium caused a decrease in percentage of dry weight of the leaf tissue culture, whereas its addition to Heller's medium had no such effect.

scholarly journals Effects of exogenous indole-3-acetic acid on the growth and cadmium accumulation of lettuce under cadmium stress

2019 ◽  
Vol 136 ◽  
pp. 07002
Author(s):  
Le Liang ◽  
Wanjia Tang ◽  
Xuemei Peng ◽  
Jing Lu ◽  
Han Liu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Dry Weight ◽  
Cadmium Stress ◽  
Optimal Dose ◽  
Cadmium Accumulation ◽  
Cd Uptake ◽  
Cd Toxicity ◽  
Substantial Decline ◽  
Indole 3 Acetic Acid ◽  
Exogenous Iaa

Indole-3-acetic acid (IAA) plays crucial roles in plant growth and stress tolerance. In present study, the effects of spraying different concentrations (0, 25, 50, 100 and 200 μmol/L) of IAA on the growth and cadmium (Cd) accumulation in lettuce (Lactuca sativa) were investigated. The lettuce exposed to Cd exhibited a substantial decline in growth, and the Cd content of them significantly increased. Spraying exogenous IAA resulted in alleviating the inhibitory of Cd toxicity to lettuce. The dry weight in shoots of lettuce increased by spraying with IAA compared with the Cd treatment alone, but the dry weight of roots had no significantly differences. Although exogenous IAA increased the root Cd content, it significantly reduced shoot Cd content, indicating its role in Cd transport. Therefore, spraying IAA effectively alleviated Cd toxicity and reduced Cd uptake in the edible parts of lettuce, and the 100 μmol/L IAA was the optimal dose.

scholarly journals Improvement of Phosphate Solubilization and Medicago Plant Yield by an Indole-3-Acetic Acid-Overproducing Strain of Sinorhizobium meliloti

2010 ◽  
Vol 76 (14) ◽  
pp. 4626-4632 ◽  
Author(s):  
Carmen Bianco ◽  
Roberto Defez
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Type Strain ◽  
Sinorhizobium Meliloti ◽  
Wild Type Strain ◽  
Dry Weight ◽  
Limiting Factors ◽  
Wild Type ◽  
System A ◽  
Indole 3 Acetic Acid

ABSTRACT Nitrogen (N) and phosphorus (P) are the most limiting factors for plant growth. Some microorganisms improve the uptake and availability of N and P, minimizing chemical fertilizer dependence. It has been published that the RD64 strain, a Sinorhizobium meliloti 1021 strain engineered to overproduce indole-3-acetic acid (IAA), showed improved nitrogen fixation ability compared to the wild-type 1021 strain. Here, we present data showing that RD64 is also highly effective in mobilizing P from insoluble sources, such as phosphate rock (PR). Under P-limiting conditions, the higher level of P-mobilizing activity of RD64 than of the 1021 wild-type strain is connected with the upregulation of genes coding for the high-affinity P transport system, the induction of acid phosphatase activity, and the increased secretion into the growth medium of malic, succinic, and fumaric acids. Medicago truncatula plants nodulated by RD64 (Mt-RD64), when grown under P-deficient conditions, released larger amounts of another P-solubilizing organic acid, 2-hydroxyglutaric acid, than plants nodulated by the wild-type strain (Mt-1021). It has already been shown that Mt-RD64 plants exhibited higher levels of dry-weight production than Mt-1021 plants. Here, we also report that P-starved Mt-RD64 plants show significant increases in both shoot and root fresh weights when compared to P-starved Mt-1021 plants. We discuss how, in a Rhizobium-legume model system, a balanced interplay of different factors linked to bacterial IAA overproduction rather than IAA production per se stimulates plant growth under stressful environmental conditions and, in particular, under P starvation.

Is there a relationship between tracheary element formation and lignification in soybean (Glycine max var. Wayne) callus cultures?

1986 ◽  
Vol 64 (11) ◽  
pp. 2716-2718 ◽  
Author(s):  
A. Raymond Miller ◽  
Lorin W. Roberts
Keyword(s):  
Acetic Acid ◽  
Glycine Max ◽  
Time Course ◽  
Lignin Content ◽  
Tracheary Element ◽  
Dry Weight ◽  
Naphthaleneacetic Acid ◽  
Tracheary Elements ◽  
Element Number ◽  
Indole 3 Acetic Acid

The possible relationship between tracheary element number and lignin content was studied in cultured soybean (Glycine max L. var. Wayne) cotyledon callus. Callus initiated on 4.5 μM 2,4-dichlorophenoxyacetic acid contained 3.0 × 104 tracheary elements per gram fresh weight and 41 μg lignin per milligram dry weight after 10 days incubation, and these values did not vary significantly after two subsequent transfers (7 days each) to a medium containing 0.1 μM α-naphthaleneacetic acid and 0.01 μM kinetin. Transfer of this callus to a medium supplemented with 60 μM indole-3-acetic acid and 0.5 μM kinetin resulted in significant increases in tracheary element number and lignin content (290 and 56%, respectively). A time-course study revealed that both tracheary element number and lignin content reached a maximum 5 to 6 days after transfer to the medium containing indole-3-acetic acid and kinetin. However, when total callus lignin content was plotted against total tracheary element number, no statistically significant relationship was found. The formation of lignin not associated with tracheary elements may have been a factor. These results indicate that the induction of tracheary element formation and lignification in soybean callus have similar hormonal requirements, but lignification occurs independently of tracheary element formation in this system.

scholarly journals Induksi Kalus Kentang Asal Desa Dombu (Solanum tuberosum L.) Dengan Zpt Indole-3-Acetic Acid (IAA)

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Lyly Zulraufianti ◽  
Asri Pirade Paserang
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Solanum Tuberosum ◽  
Natural Science ◽  
Callus Induction ◽  
Solanum Tuberosum L ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Department Faculty ◽  
Indole 3 Acetic Acid

The research of callus induction in potato (S. tuberosum  L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that  the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.

scholarly journals AUXIN ACTIVITY OF 3-METHYLENEOXINDOLE

HortScience ◽  
1992 ◽  
Vol 27 (12) ◽  
pp. 1263g-1263
Author(s):  
V. Tuli
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Biological Activity ◽  
Aqueous Solutions ◽  
Bacterial Growth ◽  
Aqueous Media ◽  
Auxin Action ◽  
Auxin Activity ◽  
Growth Assay ◽  
Indole 3 Acetic Acid

3-Methyleneoxindole (MO), a metabolite of the plant auxin 1- H-indole-3-acetic acid (IAA), is a potent sulfhydryl reagent that can profoundly affect bacterial growth and metabolism. For investigative purposes, MO is obtained from the degradation of 3-bromooxindole-3-acetic acid (3-Br-lAA) in aqueous media. Alternatively, it can be prepared from the riboflavin-catalyzed photooxidation of IAA. My earlier claims that MO possesses auxin activity were refuted by independent investigators either because the results could not be reproduced when 3-Br-IAA was used, or the results were ascribed to contamination with residual IAA if MO obtained from photooxidation was used. Recent investigations indicate that, contrary to previous assumptions, the quantitative degradation of 3-Br-lAA resulting in the formation of MO is not instantaneous; depending on the purity of 3-Br-lAA, it may take several hours to several days to reach completion. Furthermore, aqueous solutions of MO ≥0.1 mm are rapidly polymerized, thus causing a loss of biological activity. These findings may explain why MO that is derived from 3-Br-lAA often fails to produce auxin action. Ultrapure MO, obtained from either 3-Br-IAA or photooxidation, is 50- to 1000-fold as effective as IAA in the straight growth assay, induction of xylogenesis in parenchymatous tissue, and rooting of explants in tissue culture.

scholarly journals PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 157-166
Author(s):  
Apri Salfiani ◽  
Asri Pirade Paserang
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Fusarium Oxysporum ◽  
Maximum Concentration ◽  
Shoot Length ◽  
Vanilla Planifolia ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Indole 3 Acetic Acid

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).

scholarly journals Stability of IAA and IBA in Nutrient Medium to Several Tissue Culture Procedures

HortScience ◽  
1990 ◽  
Vol 25 (7) ◽  
pp. 800-802 ◽  
Author(s):  
Scott J. Nissen ◽  
Ellen G. Sutter
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Activated Charcoal ◽  
Nutrient Medium ◽  
Relative Stabilities ◽  
Murashige And Skoog Medium ◽  
Agar Media ◽  
Indole 3 Acetic Acid

The relative stabilities of IAA and IBA under various tissue culture procedures were determined. IBA was significantly more stable than IAA to autoclaving. IBA was also found to be more stable than IAA in liquid Murashige and Skoog medium (MS) under growth chamber conditions. The stabilities of IBA and IAA were similar in agar-solidified MS. Light provided by cool-white fluorescent bulbs promoted degradation of IAA and IBA in both liquid and agar media. Activated charcoal in concentrations as high as 5% was found to adsorb more than 97% of IAA and IBA in liquid MS. These results have important implications for the preparation, storage, and handling of IBA and IAA in plant tissue culture. Chemical names used: indole-3-acetic acid (IAA); indole-3-butyric acid (IBA).

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