Furanocoumarins on the surface of callus cultures from species of the Rutaceae and Umbelliferae

1993 ◽  
Vol 71 (7) ◽  
pp. 966-969 ◽  
Author(s):  
Alicja M. Zobel ◽  
Stewart A. Brown
Keyword(s):  
Cell Surface ◽  
Key Words ◽  
Daucus Carota ◽  
Callus Cultures ◽  
Ruta Graveolens ◽  
Pastinaca Sativa ◽  
Fresh Weight ◽  
Callus Cell ◽  
The Individual ◽  
Total Concentrations

Four linear furanocoumarins (psoralen, xanthotoxin, isopimpinellin, and bergapten) were identified on the surfaces of callus cells of Ruta graveolens, Ruta pinnata, Daucus carota, and Pastinaca sativa in the concentration range of 0.055 – 1.1 μg total/g fresh weight. In some cases these surface deposits amounted to a substantial percentage of the total, with the highest (67%) in Daucus, but they were not always proportional to the total concentrations in the cells. Variation in the ratios of concentrations of the individual coumarins indicated selective extrusion. An unresolved mixture of isopimpinellin and bergapten, with the former predominating in the proportion of ca. 7:1, was extruded in the highest concentrations on brown callus. Shake cultures produced up to two orders of magnitude more furanocoumarins than callus cells and extrusion was accelerated, but the concentrations of particular exported furanocoumarins varied, again pointing to selective extrusion. Key words: callus, cell surface, furanocoumarins, Ruta, Daucus, Pastinaca.

Gamma-hydroxybutyrate accumulates in green tea and soybean sprouts in response to oxygen deficiency

2003 ◽  
Vol 83 (4) ◽  
pp. 951-953 ◽  
Author(s):  
W. L Allan ◽  
C. Peiris ◽  
A. W. Bown ◽  
B. J. Shelp
Keyword(s):  
Key Words ◽  
Human Health ◽  
Green Tea ◽  
Illicit Drug ◽  
Oxygen Deficiency ◽  
Fresh Weight ◽  
Modified Atmospheres ◽  
Soybean Sprouts ◽  
Gamma Hydroxybutyrate ◽  
Bean Sprouts

Gamma-hydroxybutyrate (GHB) is an illicit drug, which may be derived in plants from a stress-inducible metabolite known as gamma-aminobutyrate. Here, oxygen deficiency caused the accumulation of GHB in green tea and soybean sprouts (76 and 155 nmol g-1 fresh weight, respectively). These findings demonstrate that GHB levels are elevated in severely modified atmospheres, but they pose no risk to human health. Further work is required to assess the extent of this phenomenon. Key words: Gamma-hydroxybutyrate, gamma-aminobutyrate, oxygen deficiency, tea, bean sprouts

scholarly journals Organogenesis of Dendrobium Orchid Using Traditional Media and Organic Extracts

1970 ◽  
pp. 30-35 ◽  
Author(s):  
S Akter ◽  
KM Nasiruddin ◽  
ABM Khaldun
Keyword(s):  
Key Words ◽  
Plantlet Regeneration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Traditional Media ◽  
Organic Extracts ◽  
Half Strength ◽  
The Media ◽  
Basal Media ◽  
Weight Number

The study was undertaken with a view to investigate the effects of different basal media and organic extracts on organogenesis of Dendrobium orchid. Protocorm like bodies (PLBs) were cultured in Knudson C (KC), Vacin and Went (VW), half strength Murashige and Skoog (½MS) and New Phalaenopsis (NP) media supplemented with Sabri banana pulp (Sb), Charcoal (C) and Coconut water (Cw) organic extracts. KC, VW, ½MS and NP media significantly influenced the plantlet regeneration of Dendrobium orchid. Among the media, ½MS showed better performance on fresh weight, number PLBs, shoots and leaves per plantlet, The longest shoots were found in KC medium and maximum length of leaves was found in both KC and ½MS media. In case of different organic extracts Sb showed significantly better performance on fresh weights of PLBs, number of shoot and leaves per explant, length of shoots and leaves. Key words: Organogenesis, Dendrobium, media, organic extracts. DOI = 10.3329/jard.v5i1.1454 J Agric Rural Dev 5(1&2), 30-35, June 2007

An evaluation of five methods for the determination of moisture in grass seeds

2002 ◽  
Vol 82 (2) ◽  
pp. 401-405
Author(s):  
N. A. Fairey
Keyword(s):  
Key Words ◽  
Rapid Determination ◽  
Performance Characteristics ◽  
Grass Species ◽  
Moisture Determination ◽  
Fresh Weight ◽  
Seed Moisture ◽  
Seed Crops ◽  
Time Of Harvest

Seed of four grass species was re-hydrated to 11 pre-determined moisture concentrations ranging from 100 to 600 g kg-1 fresh weight ( FW) to simulate seed maturation during swathing and combining. The performance characteristics of three thermogravimetric and two electronic capacitance methods of moisture determination were evaluated. The thermogravimetric methods had no moisture range limitations and were, in general, more accurate than the electronic methods. The thermogravimetric Koster tester is suitable for grass seeds of all moisture concentrations, and can be easily adapted for use at field sites. The John Deere Moisture- Chek electronic tester is suitable for the rapid determination of moisture in grass seeds but is limited to concentrations of 80–250 g kg-1 FW. Key words: Grass seed crops, seed moisture measurement, swathing, combining, time of harvest

scholarly journals Capí­tulo Segundo: Mundo Reo.

Xihmai ◽  
2013 ◽  
Vol 5 (10) ◽  
Author(s):  
Jesús Ignacio Panedas Galindo
Keyword(s):  
Key Words ◽  
Future Generations ◽  
Human Race ◽  
End Of History ◽  
Palabras Clave ◽  
The Future ◽  
The End Of History ◽  
The Individual

Resumen Cuando se empezaron a conocer los testimonios de los supervivientes de los campos de exterminio nazis, la humanidad se consternó. El sufrimiento provocado y la aplicación sistemática y consciente de la técnica a la destrucción de la persona, fueron descubrimientos que pusieron en alerta al hombre sobre la naturaleza del hombre mismo.   Tanto fue el horror que se alcanzó a entrever a través de las narraciones que el  tiempo  se  congeló.  El  reclamo  silencioso  de  las  auténticas  ví­ctimas, quienes murieron, se suspendió en el aire de la memoria hasta que los responsables reconocieran sus culpas. El olvido no podí­a abrazar tan profundos crí­menes.   Por este motivo no puede realizarse el fin de la historia. Los sufrimientos del hombre provocados hasta este grado por el mismo hombre fuerzan un pendiente que ya no puede borrarse. El grito de dolor recuerda a las generaciones futuras la necesidad de una reparación, del perdón, del reconocimiento.   Palabras Clave: Testimonio, memoria, campos de exterminio, fenomenologí­a, hermenéutica, sufrimiento, herencia.   Abstract When testimony from the survivors from Nazi extermination fields were first known, the human race filled with dismay. The suffering provoked and the systematic conscious application of the technique of destruction of the individual, were discoveries that alerted the individual on the nature of the individual itself.   Such a horror was seen through the narrations that time froze.     The silent demand from the authentic victims, who died, was suspended on the air of memory until the responsible recognized their  guilt. Obscurity could not hold such deep crimes.   For this reason the end of history cannot be made. The suffering of the individual provoked up to this point by the individual itself, force an unresolved point that cannot be erased.   The scream of pain reminds the future generations the need to repair, forgive and recognize it.   Key words: Testimony, memory, extermination fields, phenomenology, hermeneutics, suffering, inheritance.

scholarly journals INFLUENCE OF AUXIN ON IN VITRO SECONDARY METABOLITES PRODUCTION FROM BALANITES AEGYPTIACA CALLUS CULTURES

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Asmaa A.A. Abdel- Kareem ◽  
H.A. El- Shamy ◽  
A.K. Dawh ◽  
S.G. Gwiefel
Keyword(s):  
Secondary Metabolites ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ms Medium ◽  
Total Phenolic Compounds ◽  
Fresh Weight ◽  
Balanites Aegyptiaca ◽  
Metabolites Production

The present work was conducted in order to investigate the effect of auxin type (2,4-D and NAA) and concentration (0.00, 0.25, 0.50, 1.00 and 2.00 mg/l) on Balanites aegyptiaca callus cultures growth and production of secondary metabolites. Obtained results demonstrated that supplementation MS medium with 2,4-D at 2.0 mg/l could enhanced and recorded the ultimate values of callus fresh weight, antioxidant activity (%), total flavonoids, total phenolic compounds and total saponins contents and yields of Balanites aegyptiaca L. callus.

scholarly journals INDUCING SOME SECONDARY METABOLITES FROM CALLUS CULTURES DERIVED FROM Plantago psyllium AND Plantago major EXPOSED TO COBALT STRESS

2020 ◽  
Vol 51 (3) ◽  
pp. 938-943
Author(s):  
S. I. Neamah
Keyword(s):  
Secondary Metabolites ◽  
Dry Weight ◽  
Callus Cultures ◽  
Cobalt Metal ◽  
Plantago Major ◽  
Fresh Weight ◽  
Skoog Medium ◽  
Flavonoid Compounds ◽  
Metal Treatment ◽  
Plantago Psyllium

This experiment was conducted to study the influence of cobalt concentrations on the production of seven flavonoid compounds in callus derived from Plantago psyllium L. and Plantago major L. Results showed that the best combination of 2,4-D and  kinetin concentrations add to Muroshige and Skoog medium to obtain the highest fresh weight of 541.0 mg was 3.0 and 1.0 mg.L-1 respectively. psyllium stimulated callus produced the highest fresh weight of 365.7 mg. The addition of 75 ppm of cobalt resulted in a significantly lower fresh weight of P. psyllium callus (139.8 mg). The interaction between Plantago species and cobalt concentrations was significant. The callus inducted from P. major had significant increases of the scutallarein, apigenin, nepetin and luteolin compounds with 26.40, 22.64, 14.93 and 26.20 µg.100mg-1 dry weight, respectively. The production of the hispidulin compound was increased in P. psyllium at 29.40 µg.100mg-1 dry weight. Also, the addition of cobalt metal stimulated the production of flavonoids at 50 ppm cobalt producing the highest amounts of hispidulin and luteolin at 40.30 and 41.60 µg.100mg-1 dry weight, respectively. Meanwhile, 75 ppm cobalt treatment produced the highest amount of scutallarein, apigenin, nepetin and aucubin at 25.61, 23.25, 15.90 and 13.70 µg.100mg-1 dry weight, respectively. The callus inducted from P. major treated with 50 ppm of cobalt showed the highest production of scutallarein, apigenin and luteolin at 30.33, 32.26 and 51.90 µg.100mg-1 dry weight respectively. Baicalein reached 16.46 µg.100mg-1 dry weight, at 75 ppm of cobalt metal treatment in callus inducted from P. psyllium.

scholarly journals Um encontro com as fontes em História Oral

2006 ◽  
Vol 32 (1) ◽  
Author(s):  
Marcos Fábio Freire Montysuma
Keyword(s):  
Key Words ◽  
Oral History ◽  
Field Work ◽  
The Individual ◽  
The Relationship

Neste trabalho refletimos sobre a produção e os usos das fontes e dos instrumentos metodológicos que orientam as ações na condução do trabalho do historiador que lida com o que se convencionou chamar História Oral. Buscamos discutir a singularidade do trabalho de campo, envolvendo a relação entre o pesquisador e as pessoas ouvidas, como condição de uma ciência que lida essencialmente com o indivíduo. Abstract In this article we reflect upon the production and uses of sources and methodological tools which guide the actions of the historian who deals with the field conventionally referred to as Oral History. We aim at discussing the singularity of field work, bringing into play the relationship between the researcher and people who are listened to – a vital condition for a science that essentially deals with the individual. Palavras-chave: Fontes. Metodologia. História Oral. Key words: Sources. Methodology. Oral History.

Biosynthetic and cytological studies in tissue cultures and regenerated plants of haploid Atropa belladonna

1978 ◽  
Vol 56 (21) ◽  
pp. 2781-2784 ◽  
Author(s):  
Susan Eapen ◽  
T. S. Rangan ◽  
M. S. Chadha ◽  
M. R. Heble
Keyword(s):  
Callus Cultures ◽  
Haploid Plant ◽  
Tissue Cultures ◽  
Atropa Belladonna ◽  
Ploidy Levels ◽  
Total Alkaloids ◽  
Regenerated Plants ◽  
The Individual ◽  
Different Levels ◽  
Alkaloid Contents

Tissue cultures have been established from leaves of one anther-derived haploid plant of Atropa belladonna L. Regenerants obtained from callus cultures were transferred to soil and reared to maturity. Callus cells and regenerants exhibited variable degrees of ploidy. The frequency of different ploidy levels in both the systems did not vary significantly during fifth to eighth serial passages.Callus tissue and regenerated plants (at flowering stage) were analyzed to determine the concentration of tropine, atropine, scopolamine, and total alkaloids. While the alkaloid content in callus cultures was very low (0.8 × 10−3%), the regenerated plants contained different levels of individual and total alkaloids. The ploidy of the plant had direct bearing on the individual and total alkaloid contents.

Cybersex: nuove forme di dipendenza sessuale

2009 ◽  
pp. 63-78
Author(s):  
Valentina Cosmi ◽  
Luca Pierleoni ◽  
Chiara Simonelli ◽  
Adele Fabrizi
Keyword(s):  
Key Words ◽  
Internet Addiction ◽  
Sexual Addiction ◽  
Intervention Strategies ◽  
Diagnostic Classification ◽  
Sexual Dysfunctions ◽  
Industrialized Countries ◽  
Scientific Debate ◽  
The Poor ◽  
The Individual

- Sexual and internet addiction represent a rapidly growing phenomenon in the industrialized countries. The union between these clinical diseases has contributed to create new addiction forms, as cybersex addiction and cyber-relational addiction. Despite the poor diagnostic classification on this matter, the scientific debate is high pitched and thought-provoking. The different causes of these phenomenon and their heterogeneity suggest the adoption of different intervention strategies for clinical treatment of subjects with these kind of diseases, to take into account the individual needs and peculiarity.Key words: sexual addiction, internet, cybersex, compulsion, sexual dysfunctions, diagnostic criteria.Parole chiave: dipendenza sessuale, internet, sesso virtuale, compulsione, disfunzioni sessuali, criteri diagnostici.

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