Biosynthetic and cytological studies in tissue cultures and regenerated plants of haploid Atropa belladonna

1978 ◽  
Vol 56 (21) ◽  
pp. 2781-2784 ◽  
Author(s):  
Susan Eapen ◽  
T. S. Rangan ◽  
M. S. Chadha ◽  
M. R. Heble

Tissue cultures have been established from leaves of one anther-derived haploid plant of Atropa belladonna L. Regenerants obtained from callus cultures were transferred to soil and reared to maturity. Callus cells and regenerants exhibited variable degrees of ploidy. The frequency of different ploidy levels in both the systems did not vary significantly during fifth to eighth serial passages.Callus tissue and regenerated plants (at flowering stage) were analyzed to determine the concentration of tropine, atropine, scopolamine, and total alkaloids. While the alkaloid content in callus cultures was very low (0.8 × 10−3%), the regenerated plants contained different levels of individual and total alkaloids. The ploidy of the plant had direct bearing on the individual and total alkaloid contents.


Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 122-128 ◽  
Author(s):  
Michael Lee ◽  
R. L. Phillips

Chromosomal instability is a common occurrence in plant tissue cultures and has been documented in plants regenerated from several genotypes of maize (Zea mays L.) tissue cultures. The objective of this research was to evaluate the frequency and types of chromosomal aberrations in regenerated plants of an Oh43–A188 genetic background, which had not been examined previously for chromosome stability in culture. Organogenic callus cultures were intitated from immature embryos of F2 plants for several Oh43 ms isoline × A188 crosses. The chromosome constitution of 267 plants was investigated through meiotic analysis of plants regenerated either 3 to 4 or 8 to 9 months after culture initiation. No abnormalities were detected in 78 plants regenerated during the first period. During the second period, however, 91 of the 189 plants were cytologically abnormal. One hundred and eight aberrations were detected and most (96%) involved changes in chromosome structure such as interchanges (42%), deficiencies (35%), and heteromorphic pairs (19%). All deficiencies were intercalary. Also, most (51%) interchanges involved chromosome 6. An association between male-sterility factors and chromosome instability was not observed. Breakpoints were primarily on chromosome arms containing large blocks of heterochromatin such as knobs. Several abnormal plants from the same culture appeared to contain identical aberrations indicating the aberrations may trace to a single event. A hypothesis for the involvement of heterochromatin in chromosome breakage during in vitro culture is supported. Key words: Zea mays L., tissue culture, somaclonal variation, chromosome breakage, heterochromatin.



Methodology ◽  
2006 ◽  
Vol 2 (4) ◽  
pp. 142-148 ◽  
Author(s):  
Pere J. Ferrando

In the IRT person-fluctuation model, the individual trait levels fluctuate within a single test administration whereas the items have fixed locations. This article studies the relations between the person and item parameters of this model and two central properties of item and test scores: temporal stability and external validity. For temporal stability, formulas are derived for predicting and interpreting item response changes in a test-retest situation on the basis of the individual fluctuations. As for validity, formulas are derived for obtaining disattenuated estimates and for predicting changes in validity in groups with different levels of fluctuation. These latter formulas are related to previous research in the person-fit domain. The results obtained and the relations discussed are illustrated with an empirical example.



Author(s):  
Gulbarshyn Chepurko ◽  
Valerii Pylypenko

The paper examines and compares how the major sociological theories treat axiological issues. Value-driven topics are analysed in view of their relevance to society in times of crisis, when both societal life and the very structure of society undergo dramatic change. Nowadays, social scientists around the world are also witnessing such a change due to the emergence of alternative schools of sociological thought (non-classical, interpretive, postmodern, etc.) and, subsequently, the necessity to revise the paradigms that have been existed in sociology so far. Since the above-mentioned approaches are often used to address value-related issues, building a solid theoretical framework for these studies takes on considerable significance. Furthermore, the paradigm revision has been prompted by technological advances changing all areas of people’s lives, especially social interactions. The global human community, integral in nature, is being formed, and production of human values now matters more than production of things; hence the “expansion” of value-focused perspectives in contemporary sociology. The authors give special attention to collectivities which are higher-order units of the social system. These units are described as well-organised action systems where each individual performs his/her specific role. Just as the role of an individual is distinct from that of the collectivity (because the individual and the collectivity are different as units), so too a distinction is drawn between the value and the norm — because they represent different levels of social relationships. Values are the main connecting element between the society’s cultural system and the social sphere while norms, for the most part, belong to the social system. Values serve primarily to maintain the pattern according to which the society is functioning at a given time; norms are essential to social integration. Apart from being the means of regulating social processes and relationships, norms embody the “principles” that can be applied beyond a particular social system. The authors underline that it is important for Ukrainian sociology to keep abreast of the latest developments in the field of axiology and make good use of those ideas because this is a prerequisite for its successful integration into the global sociological community.



2021 ◽  
pp. 1354067X2110040
Author(s):  
Josefine Dilling ◽  
Anders Petersen

In this article, we argue that certain behaviour connected to the attempt to attain contemporary female body ideals in Denmark can be understood as an act of achievement and, thus, as an embodiment of the culture of achievement, as it is characterised in Præstationssamfundet, written by the Danish sociologist Anders Petersen (2016) Hans Reitzels Forlag . Arguing from cultural psychological and sociological standpoints, this article examines how the human body functions as a mediational tool in different ways from which the individual communicates both moral and aesthetic sociocultural ideals and values. Complex processes of embodiment, we argue, can be described with different levels of internalisation, externalisation and materialisation, where the body functions as a central mediator. Analysing the findings from a qualitative experimental study on contemporary body ideals carried out by the Danish psychologists Josefine Dilling and Maja Trillingsgaard, this article seeks to anchor such theoretical claims in central empirical findings. The main conclusions from the study are used to structure the article and build arguments on how expectations and ideals expressed in an achievement society become embodied.



Author(s):  
Maria Fedorova

The article argues that considering the individual as an economic and social actor in a socio-economic System makes it possible to look into various aspects of human development at different levels in interrelation. The main indicators of the economic and social subsystems are analyzed, which characterize the development of human potential in Russia in the period 2010-2019. A number of measures for the development of social responsibilities of the state, charity and volunteering have been substantiated.



1975 ◽  
Vol 38 (2) ◽  
pp. 300-303 ◽  
Author(s):  
S. M. Horvath ◽  
P. B. Raven ◽  
T. E. Dahms ◽  
D. J. Gray

Previous studies had indicated that maximum aerobic power (VO2 max) would be seriously impaired when HbCO levels were above 7% but was not altered if HbCO was around 2.7%. The present studies indicated that the critical level at which HbCO influenced VO2 max was approximately 4.3%. This was accompanied as in the above-noted studied with a reduction in total work time to the attainment of VO2 max. Two procedures to raise HbCO to appropriate levels were employed, i.e., a buildup wherin HbCO was incrementally increased by breathing ambient air containing 75 or 100 ppm CO and a bolus plus maintenance procedure. In the latter, HbCO was raised to the level attained in the buildup test by giving a “bolus” of CO followed by the continued inhalation of CO at a level to just maintain this level of HbCO regardless of the magnitude of the ventilation. Regardless of the mode of presentation, the decrement in VO2 max occurred at the same level of HbCO. These observations are of considerable significance, since it indicated that even low ambient levels of CO (23.7 ppm) would result in lowering maximum aerobic power if the individual had been previously exposed to CO such that the level was raised to this critical point.



1983 ◽  
Vol 73 (3) ◽  
pp. 135-149 ◽  
Author(s):  
F. Debon ◽  
P. Le Fort

ABSTRACTA classification is proposed, based mainly on major element analytical data plotted in a coherent set of three simple chemical-mineralogical diagrams. The procedure follows two complementary steps at two different levels. The first is concerned with the individual sample: the sample is given a name (e.g. granite, adamellite, granodiorite) and its chemical and mineralogical characteristics are determined. The second one is more important: it aims at defining the type of magmatic association (or series) to which the studied sample or group of samples belongs. Three main types of association are distinguished: cafemic (from source-material mainly or completely mantle-derived), aluminous (mainly or completely derived by anatexis of continental crust), and alumino-cafemic (intermediate between the other two types). Subtypes are then distinguished among the cafemic and alumino-cafemic associations: calc-alkaline (or granodioritic), subalkaline (or monzonitic), alkaline (and peralkaline), tholeiitic (or gabbroic-trondhjemitic), etc. In the same way, numerous subtypes and variants are also distinguished among the aluminous associations using a set of complementary criteria such as quartz content, colour index, alkali ratio, quartz–alkalies relationships and alumina index.Although involving a new approach using partly new criteria, this classification is consistent with most of the divisions used in previous typologies. The method may also be used in the classification of the volcanic equivalents of common plutonic rocks.



2016 ◽  
Vol 9 (1) ◽  
pp. 5-20 ◽  
Author(s):  
B.B. Velichkovsky ◽  
A.N. Gusev ◽  
V.F. Vinogradova ◽  
O.A. Arbekova

User interaction with a virtual reality system may be accompanied with a sense of presence, the illusion of reality of virtual environment. The emergence of a sense of presence is determined by both technological and psychological factors. The authors show that a sense of presence may depend on the individual characteristics of cognitive control, i.e. the system of metacognition providing cognitive system setting on the solution of specific problems in context. It was found that the expression of a feeling of presence may depend on the efficiency of the control switch functions, interference suppression and updating of working memory. At the same time, the dependence of the severity of the sense of presence on the effectiveness of cognitive control differs in virtual environments with different levels of immersion.



2019 ◽  
Vol 39 (1) ◽  
pp. 13-17
Author(s):  
S. Dmitrieva ◽  
O. Machushnyk

In current conditions, the priority of young people's preparation for life and work is especially important. Consequently, the requirements for the training of a future psychologist are also changing. One of the essential properties of a psychologist, necessary for the successful implementation of their activities, is empathy. The problem of empathy is one of the most difficult psychological sciences. The implacability of this phenomenon for researchers confirms the diversity in the definitions of its essence, mechanisms, functions, the role of empathy in the personality moral development, prosocial behaviour, altruism, and others like that. The presence of the appropriate level of empathic properties of students-psychologists acts as a condition for the formation of their professional compliance. Subjective factors of empathy formation: value sphere of personality, type of interpersonal relations, level of self-centeredness, type of accentuation of character, types of attitudes to different spheres of life, level of subjective control. Therefore, in the article, empathy development is studied in students who get a psychologist's degree. It is determined that in general subjects have average empathy level. By dividing students into groups, according to their level of empathy, it has been established that different value orientations characterize boys with different levels of empathy. It is determined that the overwhelming majority of respondents have a mean self-centeredness level. It was found that the obtained data provide an opportunity for further development of empathy among students. As a result of our research, we are convinced that the objective factors for the formation of empathy are: the perception of other people, the maturity of the individual. Our research is not exhaustive; our further development will concern the deepening of the ideas about the empathy component of the personality of the future psychologist and the methods of its development.  



2009 ◽  
Vol 4 (2) ◽  
pp. 153
Author(s):  
Muhammad Hunaina Fariduddin Ath-thar ◽  
Komar Sumantadinata ◽  
Alimuddin Alimuddin

In order to successfully perform a gene transfer activity using microinjection, an individual egg is needed. The common standard operating procedure to collect the individual egg is by applying chemical solution to remove the sticky layer covering the egg surface. Among several available solutions, tannin is one of alternative substances that can be used in performing egg microinjection on African catfish. In this study, the effect of different dosages of tannin solution to remove the sticky layer of eggs was observed. The study was conducted at the Laboratory of Fish Breeding and Genetics, Bogor Agricultural University. Prior to microinjection, the eggs were treated with different dosages of tannin solution right after fertilization. There were four different levels of dosage used as the treatments i.e.: 0.3, 0.5, 0.7, and 0.9 ppm. After the treatments, each individual egg was injected using DNA pmâactin-hrGFP plasmid with a concentration of 20 μg/mL. The results showed that the dosage of 0.5 ppm of tannin was the most effective treatment compared to the other dosages. This dosage produced 71.4% hatching rate and 57.1% transgenic embryos respectively.



Sign in / Sign up

Export Citation Format

Share Document