scholarly journals Impact of combined xylanase and β-glucanase enzymes on digesta transit time, short-chain fatty acids and caecal thermal profile of broilers fed corn-soy-based diets

2021 ◽  
Author(s):  
Guilherme Aguiar Mateus Pasquali ◽  
Tatiane Souza Santos ◽  
Leonardo Henrique Zanetti ◽  
Livia Carrasco Dornelas ◽  
Jéssica Moraes Cruvinel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Retention Time ◽  
Broiler Chickens ◽  
Short Chain Fatty Acids ◽  
Low Energy ◽  
Distal Ileum ◽  
Short Chain ◽  
Thermal Profile ◽  
Ileal Digestibility

A study was conducted to evaluate the effects of including a xylanase + β-glucanase enzyme product in corn-soy-based diets on performance, caecal short-chain fatty acids and thermal profile, ileal digestibility and intestinal kinetics of broiler chickens. A total of 744 d-old male chicks were randomly allotted to 24 floor pens and distributed in a 2 × 2 factorial arrangement with low or high energy level × without or with 100 grams of the enzyme per ton of feed. Enzyme supplementation improved bodyweight gain from 1 to 21 d. An increased caecal concentration of acetic acid was observed when the enzyme was added to the low-energy diet. The pH of the caecal content was reduced and the caecal temperature was increased by low-energy diets. The apparent ileal digestibility of energy was improved by the enzyme. The addition of the enzyme increased the mean retention time in the distal ileum. In summary, the addition of a xylanase + β-glucanase enzyme product in corn-based diets increases the retention time of digesta in the distal ileum and the caecal acetic acid concentration, improves ileal digestibility of energy and performance from 1 to 21 d in broiler chickens fed corn-soy-based diets.

scholarly journals The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids

Nutrients ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 152
Author(s):  
Kanako Omori ◽  
Hiroki Miyakawa ◽  
Aya Watanabe ◽  
Yuki Nakayama ◽  
Yijin Lyu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Dietary Fiber ◽  
Magnesium Oxide ◽  
Acid Concentration ◽  
Short Chain Fatty Acids ◽  
Water Soluble ◽  
Short Chain ◽  
Acetic Acid Concentration ◽  
Chain Fatty Acids

Constipation is a common condition that occurs in many people worldwide. While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended. Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs). SCFAs are involved in regulating systemic physiological functions and circadian rhythm. We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice. We also examined the MgO administration timing effect on cecal SCFAs. The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing. Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin. When inulin and MgO were combined, significant changes in the microbiota composition were observed compared with inulin alone. The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0. In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.

The voluntary intake of acetate by dairy cows given ammonium salts of short-chain fatty acids in their drinking water

1968 ◽  
Vol 10 (4) ◽  
pp. 473-481 ◽  
Author(s):  
P. Jackson ◽  
J. Hodgson ◽  
J. A. F. Rook
Keyword(s):  
Fatty Acids ◽  
Drinking Water ◽  
Acetic Acid ◽  
Water Intake ◽  
Daily Intake ◽  
Short Chain Fatty Acids ◽  
Sole Source ◽  
Ammonium Salts ◽  
Short Chain ◽  
Chain Fatty Acids

A solution of ammonium salts of a mixture of short-chain fatty acids (mainly acetic acid) was added to the sole source of drinking water of 10 lactating Jersey cows. There was considerable variation in the concentration of salts tolerated without depression in water intake. Some animals refused solution offered at a concentration of 0·5% (w/w) whereas one animal accepted solution at a concentration of 8% (w/w) and had a mean daily intake of salts equivalent to 836 g acetic acid.2. Adjustment of the pH of the drinking solution to 6·5–7·5 increased the tolerance to the salts solution of animals which showed a low tolerance to the unadjusted solution. A mean daily intake equivalent to 480 g acetic acid was achieved without a significant depression of water intake. Replacement of 50 % of the ammonium ions by calcium increased the intake of salts by some cows but two out of eight refused the solution at a concentration of 0·5% (w/w).3. The addition of saccharine, vanilla or aniseed to a solution of the ammonium salts gave little or no improvement in acetate intake but sodium cyclamate, ethyl acetate or molasses reduced the variability between animals in their tolerance to the solution and increased the mean intake of salts. With an addition of molasses, which gave the most marked response, there was a mean daily intake of salts equivalent to 495±26 g acetic acid.

scholarly journals Postnatal morphological development and production of short-chain fatty acids in the digestive tract of gnotobiotic piglets

2009 ◽  
Vol 54 (No. 4) ◽  
pp. 156-168 ◽  
Author(s):  
S. Gancarcikova ◽  
V. Buleca ◽  
R. Zitnan ◽  
R. Nemcova ◽  
L. Scirankova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Digestive Tract ◽  
Enterococcus Faecium ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Morphological Development ◽  
Acetoacetic Acid ◽  
Gnotobiotic Piglets ◽  
Chain Fatty Acids

The present study examined the impact of <I>Enterococcus faecium</I> on morphological development and production of short-chain fatty acids in the digestive tract of gnotobiotic piglets during milk nutrition and weaning. The experiment was carried out on (18) gnotobiotic piglets. The piglets were non-colostral and the feeding ration consisted of autoclaved milk substitute (Sanolac Ferkel, Germany). From the first day of life a probiotic strain of <I>Enterococcus faecium</I> was administered continually at a dose of 2 ml of inoculum (1 ml contained 1 × 1<sup>4</sup> CFU). The animals were weaned on Day 28. Gastrointestinal tract was collected from 18 gnotobiotic piglets slaughtered at three hours after birth and at the age of 2, 7, 14, 21, 28 and 35 days. The level of short-chain fatty acids was determined in the contents of jejunum, ileum and colon. Samples of intestinal mucosa (from duodenum, jejunum and ileum) were subjected to morphological analysis. We characterized regional variations in morphological and functional responses of the small intestine. The jejunal part of the intestinal tract of gnotobiotic piglets was characterized by relatively short crypts, extremely long villi and narrow <I>lamina propria</I> which contained only few cells up to Day 14 of life. Morphological examination showed that jejunal and ileal villi were significantly lower at 21 days of age (<I>P</I> < 0.05 and <I>P</I> < 0.001, resp.). Depending on age, the concentration of both acetoacetic acid and acetic acid was higher in the jejunal contents. The difference was significant on Day 7 of age (<I>P</I> < 0.05) for acetoacetic acid and on Day 28 of age (<I>P</I> < 0.01) for acetic acid. The concentration of acetic acid in the colonal content of gnotobiotic piglets was significantly higher on Day 7 (<I>P</I> < 0.05) and 21 of age (<I>P</I>P < 0.01). The study demonstrated that the respective bacterial species affected differently the intestinal morphology and concentration of short-chain fatty acids and suggested that postnatal bacterial colonization patterns may have long-term effects on intestinal health and development.

scholarly journals Pilot Study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols

2020 ◽  
Author(s):  
S. Habibi Goudarzi ◽  
Bill Kabat ◽  
Mark L. Cannon ◽  
Maggie Gashkoff ◽  
Rachel Zurek
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Pilot Study ◽  
Streptococcus Mutans ◽  
Headspace Analysis ◽  
Brain Heart Infusion ◽  
Short Chain Fatty Acids ◽  
Brain Heart Infusion Broth ◽  
Short Chain ◽  
Chain Fatty Acids

This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the short chain fatty acids by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for Short Chain Fatty Acid (SCFA) analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973 mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acetic acid, propionic acid, butanoic acid, pentanoic acid, ethyl butaric acid, 4-methylvaleric acid, hexanoic acid. When the Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing erythritol was used as media for this Streptococcus mutans strain, the following were produced: ethanol, acetoin, and acetic acid. Our results would suggest that constituents of the media may affect the bacterial metabolite production.

scholarly journals Pilot study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols

2020 ◽  
pp. 24-30
Author(s):  
Goudarzi S Habibi ◽  
B Kabat ◽  
M Cannon ◽  
M Gashkoff ◽  
R Zurek
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Pilot Study ◽  
Streptococcus Mutans ◽  
Headspace Analysis ◽  
Brain Heart Infusion ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Mass Selective Detector ◽  
Chain Fatty Acids

This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the Short Chain Fatty Acids (SCFA) by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for SCFA analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973-mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in BHI supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acetic acid, propionic acid, butanoic acid, pentanoic acid, ethyl butaric acid, 4-methylvaleric acid, hexanoic acid. When the BHI broth supplemented with 2% or 10% sucrose containing erythritol was used as media for this Streptococcus mutans strain, the following were produced: ethanol, acetoin, and acetic acid. Our results would indicate that constituents of the bacteria media may affect the bacterial metabolite production.

Short-Chain Fatty Acids as Sanitizers for Beef1,2,3

1980 ◽  
Vol 43 (3) ◽  
pp. 168-171 ◽  
Author(s):  
E. A. QUARTEY-PAPAFIO ◽  
R. T. MARSHALL ◽  
M. E. ANDERSON
Keyword(s):  
Fatty Acids ◽  
Ascorbic Acid ◽  
Acetic Acid ◽  
Formic Acid ◽  
Exposure Time ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Microbial Counts ◽  
Microbial Destruction ◽  
Test Cultures

Formic, acetic and propionic acids in various combinations and individually were screened for antimicrobial efficacy and effect on meat color. Microorganisms tested were two pseudomonads, three coliforms, a Streptococcus sp., a Micrococcus sp., a spore-forming bottom yeast and three film yeasts which reproduced by budding. Variables tested were microorganism, pH, concentration of sanitizer and exposure time. When the 11 cultures were exposed to individual sanitizers and mixtures of them in three replications, 2% formic acid and 1% formic plus 1% acetic acid were most effective, destroying 84 and 73%, respectively, of the test cultures. Three microorganisms that were refractory to 2% acetic acid were usually killed by 2% formic acid. Addition of ascorbic acid to the sanitizer to reduce oxidation of the meat pigments resulted in lowered microbial counts. Color was not affected by addition of 1% ascorbic acid. With added 5% ascorbic acid, discoloration was noticeable but not extensive. There was no effect of pH on microbial destruction at the concentration of acid used, However, more microorganisms were killed as time of exposure to the acid was increased.

scholarly journals Influence of the Mediterranean diet on the production of short-chain fatty acids in women at risk for breast cancer (LIBRE)

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Benjamin Seethaler ◽  
Jacqueline Beutel ◽  
Marie Kogel ◽  
Maryam Basrai ◽  
Jens Walter ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Physical Activity ◽  
Fatty Acids ◽  
Acetic Acid ◽  
Mediterranean Diet ◽  
Butyric Acid ◽  
Short Chain Fatty Acids ◽  
Valeric Acid ◽  
Short Chain ◽  
Control Group

AbstractBackground: A number of small intervention studies suggested that a Mediterranean diet (MedD) and physical activity can lower the risk for breast cancer. LIBRE is the first large multicenter RCT to test the effect of these lifestyle factors on the incidence of breast cancer in women at risk because of BRCA mutations(1). LIBRE also offers to unravel underlying mechanisms such as the role of short-chain fatty acids (SCFA) for beneficial effects of such lifestyle interventions.Methods: We examined the effect of the lifestyle intervention on the production of SCFA measured in feces by gas chromatography. From the ongoing LIBRE trial we included all complete datasets (171 women; mean age 44 ± 11 years). Both women with and without previous breast cancer diagnosis were recruited (diseased; non-diseased). The participants were randomized into an intervention group (IG) trained for MedD and physical activity, and a usual care control group (CG). Adherence to the MedD was assessed at baseline and after 3 months (V1) using the validated Mediterranean Diet Adherence Screener (MEDAS) and the EPIC food frequency questionnaire (FFQ).Results: At baseline there was no difference in SCFA levels between the groups. In the IG the MEDAS score increased substantially by 2.5 points (p < 0.001), in the CG only mildly by 0.4 points (p < 0.05). Correspondingly, the intake of fibers increased solely in the IG. In the course of the study the amount of caproic acid decreased in the control group (p < 0.001). At V1 non-diseased women showed higher amounts of acetic acid (p = 0.042), n-butyric acid (p = 0.023), n-valeric acid (p = 0.018) and iso-valeric acid (p = 0.031). There were several correlations between the intake of different fibers and fecal SCFA. For example, the sum of poly- and oligosaccharides correlated with acetic acid (p = 0.001; r = 0.316), propionic acid (p = 0.034; r = 0,251), n-butyric acid (p = 0.010; r = 0.316) and iso-valeric acid (p = 0.012; r = 0.306). There was no correlation between the MEDAS and SCFA.Discussion: A lifestyle change towards a MedD and increased physical activity did not change the levels of SCFA in feces, although an increase of fiber intake was documented in the IG. To further analyze SCFA metabolism in this target population, gut microbiota composition and function (metabolites) are currently analyzed.

scholarly journals Associations between disordered gut microbiota and changes of neurotransmitters and short-chain fatty acids in depressed mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Min Wu ◽  
Tian Tian ◽  
Qiang Mao ◽  
Tao Zou ◽  
Chan-juan Zhou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Gut Microbiota ◽  
Molecular Mechanisms ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Fecal Samples ◽  
Chain Fatty Acids

Abstract Mounting evidence suggests that gut microbiota can play an important role in pathophysiology of depression, but its specific molecular mechanisms are still unclear. This study was conducted to explore the associations between changes in neurotransmitters and short-chain fatty acids (SCFAs) and altered gut microbiota in depressed mice. Here, the chronic restraint stress (CRS) model of depression was built. The classical behavioral tests were conducted to assess the depressive-like behaviors of mice. The 16S rRNA gene sequence extracted from fecal samples was used to assess the gut microbial composition. Liquid and gas chromatography mass spectroscopy were used to identify neurotransmitters in hypothalamus and SCFAs in fecal samples, respectively. Finally, 29 differential bacteria taxa between depressed mice and control mice were identified, and the most differentially abundant bacteria taxa were genus Allobaculum and family Ruminococcaceae between the two groups. The acetic acid, propionic acid, pentanoic acid, norepinephrine, 5-HIAA and 5-HT were significantly decreased in depressed mice compared to control mice. Genus Allobaculum was found to be significantly positively correlated with acetic acid and 5-HT. Taken together, these results provided novel microbial and metabolic frameworks for understanding the role of microbiota-gut-brain axis in depression, and suggested new insights to pave the way for novel therapeutic methods.

scholarly journals Effect of Oat Hulls Incorporated in the Diet or Fed as Free Choice on Growth Performance, Carcass Yield, Gut Morphology and Digesta Short Chain Fatty Acids of Broiler Chickens

2020 ◽  
Vol 12 (9) ◽  
pp. 3744 ◽  
Author(s):  
Deborah Adewole ◽  
Janice MacIsaac ◽  
Gillian Fraser ◽  
Bruce Rathgeber
Keyword(s):  
Fatty Acids ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Free Choice ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Carcass Yield ◽  
Oat Hulls ◽  
Treatment Groups ◽  
Chain Fatty Acids

The use of high fiber agricultural byproducts in poultry nutrition can help not only to reduce feed cost but also to promote gastrointestinal functionality and growth performance and enhance environmental sustainability. This study was conducted to examine the effect of oat hulls (OH) incorporated in the diet or fed as free choice on growth performance, intestinal morphology, cecal short chain fatty acids (SCFA) production and carcass yield of broiler chickens. Day old broiler chickens were assigned to 4 dietary treatment groups which consisted of a corn–soybean meal–wheat based diet (Basal), Basal + bacitracin methylene disalicylate (BMD), Basal + 3% OH (3% OH) and Basal + free choice OH (FCOH). Each group had six replicate pens of 27 chicks and were raised for 39 d. Feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) were determined weekly for each pen. One chicken/pen was slaughtered on d 36 to determine organ weights, jejunal morphology, digesta pH and cecal SCFA and two chickens/pen were slaughtered on d 39 to determine carcass weight and yield. Diet had no effect on FI, except on d 28, when the FCOH group had higher FI than the 3% OH groups. During the starter phase, chickens fed 3% OH had the highest BWG and lowest FCR, which were significantly different (p < 0.05) from those fed FCOH. Chickens fed 3% OH had the highest slaughter, hot carcass, and cold carcass weights, which were significantly different (p < 0.05) from those fed FCOH. Diet had no effect on jejunal villus height and crypt depth, ileal and cecal digesta pH, and cecal SCFA. Chickens fed FCOH had a larger (p < 0.0001) gizzard weight and showed a tendency to have a larger (p = 0.09) ceca weight than other treatment groups. In conclusion, the inclusion of 3% OH in an antibiotic-free diet has the potential to enhance growth performance, while free choice OH increases the gizzard weight of broiler chickens.

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