Effects of ABA and NaCl on physiological responses in selected bryophyte species

The effects of NaCl and abscisic acid (ABA) on selected bryophyte species were studied. Two phylogenetically unrelated halophyte mosses, namely, Entosthodon hungaricus (Boros) Loeske and Hennediella heimii (Hedw.) R.H. Zander in addition to one model non-halophyte moss, Physcomitrella patens (Hedw.) Bruch & Schimp, were selected to compare the variability in certain biochemical and physiological parameters under salt-stress alone and salt-stress upon ABA pretreatment. The results showed different patterns of effects from ABA in all three of the studied species, as well as no common response to salt stress. In general, all of the tested species reacted to exogenous ABA, which definitely contributed to changes observed in morphological development under salt stress, and to the functioning of the salt-tolerance mechanisms. Physcomitrella patens proved to be a salt-tolerant species. Although it is not ecologically classified as a halophyte, these results highlighted that various stress-resistance pathways are supported by similar reactions to different stresses. Significant differences in stress tolerance were documented between the two bryo-halophytes tested by comparing biochemical and physiological parameters. Our findings suggest that different salt-stress-tolerance strategies characterize these two species, both enhanced by exogenous ABA.

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Temporal salt stress-induced transcriptome alterations and regulatory mechanisms revealed by PacBio long-reads RNA sequencing in Gossypium hirsutum

BMC Genomics ◽

10.1186/s12864-020-07260-z ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Delong Wang ◽

Xuke Lu ◽

Xiugui Chen ◽

Shuai Wang ◽

Junjuan Wang ◽

...

Keyword(s):

Superoxide Dismutase ◽

Salt Stress ◽

Stress Response ◽

Salt Tolerance ◽

Gossypium Hirsutum ◽

Stress Tolerance ◽

Economic Losses ◽

Salt Tolerant ◽

Salt Stress Tolerance ◽

Long Reads

Abstract Background Cotton (Gossypium hirsutum) is considered a fairly salt tolerant crop however, salinity can still cause significant economic losses by affecting the yield and deteriorating the fiber quality. We studied a salt-tolerant upland cotton cultivar under temporal salt stress to unfold the salt tolerance molecular mechanisms. Biochemical response to salt stress (400 mM) was measured at 0 h, 3 h, 12 h, 24 h and 48 h post stress intervals and single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) combined with the unique molecular identifiers approach was used to identify differentially expressed genes (DEG). Results Antioxidant enzymes including, catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) were found significantly induced under temporal salt stress, suggesting that reactive oxygen species scavenging antioxidant machinery is an essential component of salt tolerance mechanism in cotton. We identified a wealth of novel transcripts based on the PacBio long reads sequencing approach. Prolonged salt stress duration induces high number of DEGs. Significant numbers of DEGs were found under key terms related to stress pathways such as “response to oxidative stress”, “response to salt stress”, “response to water deprivation”, “cation transport”, “metal ion transport”, “superoxide dismutase”, and “reductase”. Key DEGs related to hormone (abscisic acid, ethylene and jasmonic acid) biosynthesis, ion homeostasis (CBL-interacting serine/threonine-protein kinase genes, calcium-binding proteins, potassium transporter genes, potassium channel genes, sodium/hydrogen exchanger or antiporter genes), antioxidant activity (POD, SOD, CAT, glutathione reductase), transcription factors (myeloblastosis, WRKY, Apetala 2) and cell wall modification were found highly active in response to salt stress in cotton. Expression fold change of these DEGs showed both positive and negative responses, highlighting the complex nature of salt stress tolerance mechanisms in cotton. Conclusion Collectively, this study provides a good insight into the regulatory mechanism under salt stress in cotton and lays the foundation for further improvement of salt stress tolerance.

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Comparative physiological and transcriptomic analyses provide integrated insight into osmotic, cold, and salt stress tolerance mechanisms in banana

Scientific Reports ◽

10.1038/srep43007 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 17

Author(s):

Wei Hu ◽

Zehong Ding ◽

Weiwei Tie ◽

Yan Yan ◽

Yang Liu ◽

...

Keyword(s):

Salt Stress ◽

Stress Tolerance ◽

Salt Stress Tolerance ◽

Tolerance Mechanisms ◽

Insight Into

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In vitro screening for salt stress tolerance of native and exotic potato genotypes by morphological and physiological parameters

Journal of Bio-Science ◽

10.3329/jbs.v28i0.44707 ◽

2019 ◽

Vol 28 ◽

pp. 21-32 ◽

Cited By ~ 2

Author(s):

M Harun Or Rashid ◽

SMS Islam ◽

MA Bari

Keyword(s):

Salt Stress ◽

Stress Tolerance ◽

Root Length ◽

Dry Weight ◽

Physiological Parameters ◽

Stem Length ◽

In Vitro Screening ◽

Salt Tolerant ◽

Whole Plant

This study was performed on in vitro bioassay screening for salt tolerance of ten native and six exotic potato genotypes in Bangladesh. Single node was used to evaluate salinity tolerance especially on biomass production. Five different concentrations of NaCl (0 = control, 100, 150, 200 and 250 mM) were used in addition to MS medium and evaluated salt tolerant and sensitive genotype by various morphological and physiological parameters e.g. shoot and root length and its thickness, number of leaves and roots, fresh and dry weight of whole plant and water contents. The ANOVA, DMRT and correlation coefficient were found highly significant at p<0.01 among the genotypes. With salt stress condition highly positive correlation, co-efficient were found between stem length and internodal distance, leaves number, roots number, root length, dry weight of whole plant and fresh weight of whole plant. A dendrogram based on relative values of 10 morphological and physiological parameters of growth under salt conditions were led to clustering into four distinct group’s i.e. tolerant, moderately tolerant, sensitive and very sensitive. On the basis of stress tolerance trait indices (STTIs), Arun (92.78) and Ausha (80.27) showed as a highest salt tolerant, Jamalu (56.33) and Chollisha (57.03) showed the most salt sensitive potato cultivars. From this finding it may be concluded that in vitro screening with bioassay are relatively simple, rapid and convenient and these methods can be used for further advance biotechnological research on potato improvement. J. bio-sci. 28: 21-32, 2020

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Quantitative Imaging Reveals Distinct Contributions of SnRK2 and ABI3 in Plasmodesmatal Permeability in Physcomitrella patens

Plant and Cell Physiology ◽

10.1093/pcp/pcaa021 ◽

2020 ◽

Vol 61 (5) ◽

pp. 942-956 ◽

Cited By ~ 2

Author(s):

Takumi Tomoi ◽

Kensuke Kawade ◽

Munenori Kitagawa ◽

Yoichi Sakata ◽

Hirokazu Tsukaya ◽

...

Keyword(s):

Salt Stress ◽

Stress Tolerance ◽

Abiotic Stresses ◽

Physcomitrella Patens ◽

Quantitative Imaging ◽

Cell Communication ◽

Aba Signaling ◽

Salt Stress Tolerance ◽

Callose Deposition ◽

Signaling Components

Abstract Cell-to-cell communication is tightly regulated in response to environmental stimuli in plants. We previously used a photoconvertible fluorescent protein Dendra2 as a model reporter to study this process. This experiment revealed that macromolecular trafficking between protonemal cells in Physcomitrella patens is suppressed in response to abscisic acid (ABA). However, it remains unknown which ABA signaling components contribute to this suppression and how. Here, we show that ABA signaling components SUCROSE NON-FERMENTING 1-RELATED PROTEIN KINASE 2 (PpSnRK2) and ABA INSENSITIVE 3 (PpABI3) play roles as an essential and promotive factor, respectively, in regulating ABA-induced suppression of Dendra2 diffusion between cells (ASD). Our quantitative imaging analysis revealed that disruption of PpSnRK2 resulted in defective ASD onset itself, whereas disruption of PpABI3 caused an 81-min delay in the initiation of ASD. Live-cell imaging of callose deposition using aniline blue staining showed that, despite this onset delay, callose deposition on cross walls remained constant in the PpABI3 disruptant, suggesting that PpABI3 facilitates ASD in a callose-independent manner. Given that ABA is an important phytohormone to cope with abiotic stresses, we further explored cellular physiological responses. We found that the acquisition of salt stress tolerance is promoted by PpABI3 in a quantitative manner similar to ASD. Our results suggest that PpABI3-mediated ABA signaling may effectively coordinate cell-to-cell communication during the acquisition of salt stress tolerance. This study will accelerate the quantitative study for ABA signaling mechanism and function in response to various abiotic stresses.

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