Study of horseradish peroxidase and hydrogen peroxide bi-analyte sensor with boronate affinity-based molecularly imprinted film

2019 ◽  
Vol 97 (12) ◽  
pp. 833-839 ◽  
Author(s):  
Shaoming Yang ◽  
Chaopeng Bai ◽  
Yu Teng ◽  
Jian Zhang ◽  
Jiaxi Peng ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Current Response ◽  
Electron Mediator ◽  
Molecularly Imprinted ◽  
Recognition Element ◽  
Linear Relationships ◽  
Mip Sensor ◽  
Molecularly Imprinted Film

A novel electrochemical horseradish peroxidase (HRP) sensor was developed based on boronate affinity-based electropolymerized polythionine (PTh) molecularly imprinted polymer (MIP) as specific recognition element for HRP on gold nanoparticles (AuNPs) modified glassy carbon electrode, in which PTh acted as the electrochemical probe for the sensor. The sensor was characterized by scanning electron microscopy and electron dispersive spectroscopy. Electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry were exploited for the study of the properties of the MIP sensor. The MIP sensor exhibited excellent linear response over the range of 2.0 × 10−10 mg/mL ∼ 1.0 × 10−7 mg/mL for HRP. In addition, with MIP film as HRP immobilized matrices, the sensor for the detection of H2O2 was developed with the MIP sensor based on the reduction of H2O2 catalyzed by HRP in the presence of electron mediator PTh. The sensor showed linear relationships between the current response and H2O2 concentration from 6.0 × 10−7 to 2.0 × 10−5 mol/L. HRP and H2O2 bi-analyte sensor based on MIP film was successfully developed in this work. The developed method can also be applicable for enzyme and its enzymatic substrate bi-analyte sensor.

scholarly journals Low Cost, Easy to Prepare and Disposable Electrochemical Molecularly Imprinted Sensor for Diclofenac Detection

Sensors ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 1975
Author(s):  
Isabel Seguro ◽  
João G. Pacheco ◽  
Cristina Delerue-Matos
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Tap Water ◽  
Low Cost ◽  
Differential Pulse ◽  
Portable Devices ◽  
Molecularly Imprinted ◽  
Selective Determination ◽  
Mip Sensor

In this work, a disposable electrochemical (voltammetric) molecularly imprinted polymer (MIP) sensor for the selective determination of diclofenac (DCF) was constructed. The proposed MIP-sensor permits fast (30 min) analysis, is cheap, easy to prepare and has the potential to be integrated with portable devices. Due to its simplicity and efficiency, surface imprinting by electropolymerization was used to prepare a MIP on a screen-printed carbon electrode (SPCE). MIP preparation was achieved by cyclic voltammetry (CV), using dopamine (DA) as a monomer in the presence of DCF. The differential pulse voltammetry (DPV) detection of DCF at MIP/SPCE and non-imprinted control sensors (NIP) showed an imprinting factor of 2.5. Several experimental preparation parameters were studied and optimized. CV and electrochemical impedance spectroscopy (EIS) experiments were performed to evaluate the electrode surface modifications. The MIP sensor showed adequate selectivity (in comparison with other drug molecules), intra-day repeatability of 7.5%, inter-day repeatability of 11.5%, a linear range between 0.1 and 10 μM (r2 = 0.9963) and a limit of detection (LOD) and quantification (LOQ) of 70 and 200 nM, respectively. Its applicability was successfully demonstrated by the determination of DCF in spiked water samples (river and tap water).

Electrochemical Detection of Fenthion Insecticide in Olive Oils by a Sensitive Non-Enzymatic Biomimetic Sensor Enhanced with Metal Nanoparticles

2021 ◽  
Vol 5 (1) ◽  
pp. 64
Author(s):  
Youssra Aghoutane ◽  
Nezha El Bari ◽  
Zoubida Laghrari ◽  
Benachir Bouchikhi
Keyword(s):  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Organophosphate Insecticide ◽  
Imprinted Polymer ◽  
High Recovery ◽  
Molecularly Imprinted ◽  
Olive Oils ◽  
Pulse Voltammetry ◽  
Biomimetic Sensor

Fenthion, an organophosphate insecticide, is a cholinesterase inhibitor and is highly toxic. An electrochemical sensor based on molecularly imprinted polymer is developed here for its detection. For this purpose, 2-aminothiophenol mixed with gold nanoparticles was immobilized on screen-printed gold electrodes. The FEN pattern was then fixed before being covered with 2-aminothiophenol. Cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy methods were used for the electrochemical characterization. The low detection limit was 0.05 mg/Kg over a range of 0.01–17.3 µg/mL. The sensor was successfully applied for the determination of FEN in olive oil samples with high recovery values.

Development of Melamine Electrochemical Sensor Using Molecularly Imprinted Conducting Polyanilne-Oxalic Acid Blend as a Molecular Recognition Element

2020 ◽  
Vol 29 ◽  
pp. 61-73
Author(s):  
Melkamu Biyana Regasa ◽  
Olu Emmanuel Femi ◽  
Tesfaye Refera Soreta ◽  
Praveen C. Ramamarthy ◽  
Saravanan Kumaran
Keyword(s):  
Molecular Recognition ◽  
Oxalic Acid ◽  
Electrochemical Polymerization ◽  
Differential Pulse ◽  
Molar Ratio ◽  
Imprinted Polymer ◽  
Formula Milk ◽  
Molecularly Imprinted ◽  
Practical Applications ◽  
Recognition Element

In the present work, oxalic acid doped molecularly imprinted conducting polyaniline film for melamine detection was prepared by in-situ-electrochemical polymerization on the glassy carbon electrode (GCE) using melamine as template. The optimal monomer/template molar ratio was attained to be 0.2:0.1:0.01 (aniline: oxalic acid: melamine) and molecular recognition properties towards melamine were evaluated by differential pulse voltammetry. Under optimal conditions the imprinted polymer film was used to detect different concentrations of melamine in standard solutions and real milk samples. Compared with the nonimprinted polymer (NIP), the molecularly imprinted polymer (MIP) film showed higher affinity and sensitivity towards melamine with a linear range, quantification limit and detection limit of 0.5-200 nM, 1.375 nM and 0.413 nM respectively. Furthermore, the polymer blend film showed good selectivity toward melamine, stability, reproducibility and practical applications for the determination of melamine in infant formula milk with the recovery of 92.32-102.49%. The doping of the polymer with oxalic acid enhanced the conductivity and sensitivity of the sensor.

scholarly journals Novel Electrochemical Sensor Fabricated for Individual and Simultaneous Ultrasensitive Determination of Olaquindox and Carbadox Based on MWCNT-OH/CMK-8 Hybrid Nanocomposite Film

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3041 ◽  
Author(s):  
Yanqing Liu ◽  
Gengxin Hu ◽  
Hongwu Wang ◽  
Su Yao ◽  
Yinjian Ye
Keyword(s):  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Modified Electrode ◽  
Electrochemical Impedance ◽  
Modified Electrodes ◽  
Differential Pulse ◽  
Hybrid Nanocomposite ◽  
Experimental Conditions ◽  
Linear Relationships

A hybrid nanocomposite consisting of hydroxylated multi-walled carbon nanotubes (MWCNTs−OH) and cube mesoporous carbon (CMK−8) was applied in this study to construct an MWCNT−OH/CMK−8/gold electrode (GE) electrochemical sensor and simultaneously perform the electro-reduction of olaquindox (OLA) and carbadox (CBX). The respective peak currents of CBX and OLA on the modified electrode increased by 720- and 595-fold relative to the peak current of GE. The performances of the modified electrode were investigated with electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry. Then, the modified electrodes were used for the individual and simultaneous determination of OLA and CBX. The fabricated sensor demonstrated a linear response at 0.2–500 nmol/L in optimum experimental conditions, and the detection limits were 104.1 and 62.9 pmol/L for the simultaneous determination of OLA and CBX, respectively. As for individual determination, wide linear relationships were obtained for the detected OLA with levels of 0.05–500 nmol/L with LOD of 20.7 pmol/L and the detected CBX with levels of 0.10–500 nmol/L with LOD of 50.2 pmol/L. The fabricated sensor was successfully used in the independent and simultaneous determination of OLA and CBX in spiked pork samples.

Construction and Application of Molecularly Imprinted Film Sensor on Determination of Chlorpyrifos in Water

2014 ◽  
Vol 936 ◽  
pp. 843-849 ◽  
Author(s):  
Gui Feng Lin ◽  
Yan Hong Wang ◽  
Guo Chen Li ◽  
Wen Bai ◽  
Hong Zhang ◽  
...  
Keyword(s):  
High Selectivity ◽  
Constant Current ◽  
Film Sensor ◽  
Molecularly Imprinted ◽  
Binding Characteristics ◽  
Response Characteristics ◽  
Recognition Element ◽  
Sensitive Film ◽  
Molecularly Imprinted Film

Molecularly imprinted microspheres for recognition of chlorpyrifos were prepared by thermal initiation and precipitation polymerization, and were characterized using scanning electron microscope and infrared spectroscopy. Binding characteristics of the microspheres were also investigated. Sensitive film electrode of chlorpyrifos was prepared using the microspheres above on the surface of gold electrode by spin coating. Electrochemical sensor was constructed with the electrode prepared above as recognition element, and the response characteristics of the sensor to chlorpyrifos in water were investigated based on constant-current potentiometry. It was shown that a reasonable linear response curve between potential and concentration was obtained from 1.0×10-12mol/L to 2.0×10-8mol/L, with a detection limit of 1.0×10-13mol/L. The suitable pH was 2.2~3.4,and response time was 10 min. The imprinted electrode showed relatively high selectivity to chlorpyrifos and was applied to the analysis of chlorpyrifos in the simulated river samples with recovery rates ranging from 89% to 105%.

scholarly journals Redesigning an Electrochemical MIP Sensor for PFOS: Practicalities and Pitfalls

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4433 ◽  
Author(s):  
Giulia Moro ◽  
Davide Cristofori ◽  
Fabio Bottari ◽  
Elti Cattaruzza ◽  
Karolien De Wael ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Electrochemical Sensors ◽  
Differential Pulse ◽  
Complete Characterization ◽  
In Situ Monitoring ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Heterogeneous Distribution ◽  
Molecularly Imprinted ◽  
Mip Sensor

There is a growing interest in the technological transfer of highly performing electrochemical sensors within portable analytical devices for the in situ monitoring of environmental contaminants, such as perfluorooctanesulfonic acid (PFOS). In the redesign of biomimetic sensors, many parameters should be taken into account from the working conditions to the electrode surface roughness. A complete characterization of the surface modifiers can help to avoid time-consuming optimizations and better interpret the sensor responses. In the present study, a molecularly imprinted polymer electrochemical sensor (MIP) for PFOS optimized on gold disk electrodes was redesigned on commercial gold screen-printed electrodes. However, its performance investigated by differential pulse voltammetry was found to be poor. Before proceeding with further optimization, a morphological study of the bare and modified electrode surfaces was carried out by scanning electron microscopy–energy-dispersive X-ray spectrometry (SEM–EDS), atomic force microscopy (AFM) and profilometry revealing an heterogeneous distribution of the polymer strongly influenced by the electrode roughness. The high content of fluorine of the target-template molecule allowed to map the distribution of the molecularly imprinted polymer before the template removal and to define a characterization protocol. This case study shows the importance of a multi-analytical characterization approach and identify significant parameters to be considered in similar redesigning studies.

scholarly journals A “Single-Use” Ceramic-Based Electrochemical Sensor Chip Using Molecularly Imprinted Carbon Paste Electrode

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5847
Author(s):  
Aaryashree ◽  
Yuuto Takeda ◽  
Momoe Kanai ◽  
Akihiko Hatano ◽  
Yasuo Yoshimi ◽  
...  
Keyword(s):  
Differential Pulse ◽  
Sensor Chip ◽  
Therapeutic Drug ◽  
Carbon Paste ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Single Use ◽  
Mip Sensor ◽  
Response Current ◽  
Time Required

An inexpensive disposable electrochemical drug sensor for the detection of drugs (vancomycin, meropenem, theophylline, and phenobarbital) is described. Molecularly imprinted polymer (MIP) templated with the target drugs was immobilized on the surface of graphite particles using a simple radical polymerization method and packed into the working electrode of a three-electrode ceramic-based chip sensor. Differential pulse voltammetry (DPV) was used to determine the relationship between the response current and the concentration of the targeted drug while using one sensor chip for one single operation. The time required for each DPV measurement was less than 2 min. Concentrations corresponding to the therapeutic range of these drugs in plasma were taken into account while performing DPV. In all the cases, the single-used MIP sensor showed higher sensitivity and linearity than non-imprinted polymer. The selectivity test in drugs with a structure similar to that of the target drugs was performed, and it was found that MIP-based sensors were more selective than the untreated ones. Additionally, the test in whole blood showed that the presence of interfering species had an insignificant effect on the diagnostic responses of the sensor. These results demonstrate that the disposable MIP-sensor is promising for quick and straightforward therapeutic drug monitoring to prevent the toxic side effects and the insufficient therapeutic effect due to the overdose and underdose, respectively.

scholarly journals Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin

Biosensors ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 31 ◽  
Author(s):  
Bogdan Feier ◽  
Adrian Blidar ◽  
Alexandra Pusta ◽  
Paula Carciuc ◽  
Cecilia Cristea
Keyword(s):  
Electrochemical Sensor ◽  
Molecularly Imprinted Polymer ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Boron Doped Diamond ◽  
Molecularly Imprinted ◽  
Diamond Electrode ◽  
The Difference ◽  
Mip Sensor

In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.

Graphene Modified Molecular Imprinted Electrochemical Sensor for Specific Recognition of Bovine Serum Albumin

2013 ◽  
Vol 709 ◽  
pp. 891-894 ◽  
Author(s):  
Feng Li ◽  
Jian Fei Xia ◽  
Zong Hua Wang ◽  
Yan Zhi Xia ◽  
Fei Fei Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Electrochemical Impedance ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Modified Glassy Carbon Electrode ◽  
Molecularly Imprinted ◽  
Active Probe ◽  
Pulse Voltammetry

A simple and efficient molecularly imprinted sensor (MIPs/GR/GCE) was firstly prepared by electropolymerization of pyrrole in the presence of bovine serum albumin (BSA) in an aqueous solution based on a graphene modified glassy carbon electrode for the selective recognition of bovine serum albumin. The prepared sensor was characterized by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS), in which [Fe(CN)6]3−/4−was used as an electrochemical active probe. The results showed a wide linear range from 1.0 × 10-3to 1.0 × 10-9g/mL. And the imprinted biosensor indicated excellent selectivity and high sensitivity.

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