Characteristics and Identification of Pasteurella and Vibrio Species Pathogenic to Fishes using API-20E (Analytab Products) Multitube Test Strips

1982 ◽  
Vol 39 (12) ◽  
pp. 1725-1729 ◽  
Author(s):  
Michael L. Kent
Keyword(s):  
Vibrio Anguillarum ◽  
Diagnostic Procedures ◽  
Blood Agar ◽  
Marine Agar ◽  
Biochemical Characteristics ◽  
Test Strips ◽  
Fish Pathology ◽  
Fish Bacteria ◽  
Vibrio Spp

Morphological, cultural, and biochemical characteristics of Pasteurella spp. and Vibrio spp. pathogenic to fishes are investigated. Vibrio anguillarum, V. ordalii, V. alginolyticus, V. parahaemolyticus, an unidentified Vibrio sp. (AN1K), Pasteurella piscicida, and P. multocida were cultured on API-20E multitube test strips (Analytab Products) and in Baumann's marine media with similar results. The API-20E Profile Index was not useful for classification of fish bacteria studied and, using the index. Vibrio sp. AN1K was misidentified as P. multocida. Bacteria studied were also grown on Marine Agar (Difco) and on blood agar at 25 and 37 °C. On blood agar at 37 °C some vibrios resembled pasteurellas as a result of pleomorphism and lack of motility. Simple diagnostic procedures for identifying Pasteurella spp. and Vibrio spp. associated with fishes using the API-20E with modifications are presented.Key words: Pasteurella, Vibrio, characteristics, Analytical Profile Index, fish pathology

scholarly journals Recent Development toward the Next Clinical Practice of Primary Aldosteronism: A Literature Review

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 310
Author(s):  
Yuta Tezuka ◽  
Yuto Yamazaki ◽  
Yasuhiro Nakamura ◽  
Hironobu Sasano ◽  
Fumitoshi Satoh
Keyword(s):  
Clinical Practice ◽  
Primary Aldosteronism ◽  
Diagnostic Procedures ◽  
Secondary Hypertension ◽  
Refractory Hypertension ◽  
Diagnostic Biomarkers ◽  
Histopathological Classification ◽  
Subtype Differentiation ◽  
Work Up

For the last seven decades, primary aldosteronism (PA) has been gradually recognized as a leading cause of secondary hypertension harboring increased risks of cardiovascular incidents compared to essential hypertension. Clinically, PA consists of two major subtypes, surgically curable and uncurable phenotypes, determined as unilateral or bilateral PA by adrenal venous sampling. In order to further optimize the treatment, surgery or medications, diagnostic procedures from screening to subtype differentiation is indispensable, while in the general clinical practice, the work-up rate is extremely low even in the patients with refractory hypertension because of the time-consuming and labor-intensive nature of the procedures. Therefore, a novel tool to simplify the diagnostic flow has been recently in enormous demand. In this review, we focus on recent progress in the following clinically important topics of PA: prevalence of PA and its subtypes, newly revealed histopathological classification of aldosterone-producing lesions, novel diagnostic biomarkers and prediction scores. More effective strategy to diagnose PA based on better understanding of its epidemiology and pathology should lead to early detection of PA and could decrease the cardiovascular and renal complications of the patients.

scholarly journals Flow Cytometry Immunophenotyping for Diagnostic Orientation and Classification of Pediatric Cancer Based on the EuroFlow Solid Tumor Orientation Tube (STOT)

Cancers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 4945
Author(s):  
Cristiane de Sá de Sá Ferreira-Facio ◽  
Vitor Botafogo ◽  
Patrícia Mello Ferrão ◽  
Maria Clara Canellas ◽  
Cristiane B. Milito ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Solid Tumors ◽  
Solid Tumor ◽  
Pediatric Cancer ◽  
Diagnostic Procedures ◽  
Hematologic Malignancies ◽  
Multiparameter Flow Cytometry ◽  
Diagnostic Screening ◽  
Pediatric Solid Tumors

Early diagnosis of pediatric cancer is key for adequate patient management and improved outcome. Although multiparameter flow cytometry (MFC) has proven of great utility in the diagnosis and classification of hematologic malignancies, its application to non-hematopoietic pediatric tumors remains limited. Here we designed and prospectively validated a new single eight-color antibody combination—solid tumor orientation tube, STOT—for diagnostic screening of pediatric cancer by MFC. A total of 476 samples (139 tumor mass, 138 bone marrow, 86 lymph node, 58 peripheral blood, and 55 other body fluid samples) from 296 patients with diagnostic suspicion of pediatric cancer were analyzed by MFC vs. conventional diagnostic procedures. STOT was designed after several design–test–evaluate–redesign cycles based on a large panel of monoclonal antibody combinations tested on 301 samples. In its final version, STOT consists of a single 8-color/12-marker antibody combination (CD99-CD8/numyogenin/CD4-EpCAM/CD56/GD2/smCD3-CD19/cyCD3-CD271/CD45). Prospective validation of STOT in 149 samples showed concordant results with the patient WHO/ICCC-3 diagnosis in 138/149 cases (92.6%). These included: 63/63 (100%) reactive/disease-free samples, 43/44 (98%) malignant and 4/4 (100%) benign non-hematopoietic tumors together with 28/38 (74%) leukemia/lymphoma cases; the only exception was Hodgkin lymphoma that required additional markers to be stained. In addition, STOT allowed accurate discrimination among the four most common subtypes of malignant CD45− CD56++ non-hematopoietic solid tumors: 13/13 (GD2++ numyogenin− CD271−/+ nuMyoD1− CD99− EpCAM−) neuroblastoma samples, 5/5 (GD2− numyogenin++ CD271++ nuMyoD1++ CD99−/+ EpCAM−) rhabdomyosarcomas, 2/2 (GD2−/+ numyogenin− CD271+ nuMyoD1− CD99+ EpCAM−) Ewing sarcoma family of tumors, and 7/7 (GD2− numyogenin− CD271+ nuMyoD1− CD99− EpCAM+) Wilms tumors. In summary, here we designed and validated a new standardized antibody combination and MFC assay for diagnostic screening of pediatric solid tumors that might contribute to fast and accurate diagnostic orientation and classification of pediatric cancer in routine clinical practice.

scholarly journals Surgical Management, Preoperative Tumor Localization, and Histopathology of 80 Patients Operated on for Insulinoma

2019 ◽  
Vol 104 (12) ◽  
pp. 6129-6138 ◽  
Author(s):  
Mikkel Andreassen ◽  
Emma Ilett ◽  
Dominik Wiese ◽  
Emily P Slater ◽  
Marianne Klose ◽  
...  
Keyword(s):  
Malignant Tumors ◽  
Somatostatin Receptor ◽  
Diagnostic Procedures ◽  
Benign Tumors ◽  
Receptor Subtype ◽  
Receptor Imaging ◽  
Venous Sampling ◽  
Transabdominal Ultrasonography ◽  
Positron Emission

Abstract Introduction Diagnosis and pathological classification of insulinomas are challenging. Aim To characterize localization of tumors, surgery outcomes, and histopathology in patients with insulinoma. Methods Patients with surgically resected sporadic insulinoma were included. Results Eighty patients were included. Seven had a malignant tumor. A total of 312 diagnostic examinations were performed: endoscopic ultrasonography (EUS; n = 59; sensitivity, 70%), MRI (n = 33; sensitivity, 58%), CT (n = 55; sensitivity, 47%), transabdominal ultrasonography (US; n = 45; sensitivity, 40%), somatostatin receptor imaging (n = 17; sensitivity, 29%), 18F-fluorodeoxyglucose positron emission tomography/CT (n = 1; negative), percutaneous transhepatic venous sampling (n = 10; sensitivity, 90%), arterial stimulation venous sampling (n = 20; sensitivity, 65%), and intraoperative US (n = 72; sensitivity, 89%). Fourteen tumors could not be visualized. Invasive methods were used in 7 of these 14 patients and localized the tumor in all cases. Median tumor size was 15 mm (range, 7 to 80 mm). Tumors with malignant vs benign behavior showed less staining for insulin (3 of 7 vs 66 of 73; P = 0.015) and for proinsulin (3 of 6 vs 58 of 59; P < 0.001). Staining for glucagon was seen in 2 of 6 malignant tumors and in no benign tumors (P < 0.001). Forty-three insulinomas stained negative for somatostatin receptor subtype 2a. Conclusion Localization of insulinomas requires many different diagnostic procedures. Most tumors can be localized by conventional imaging, including EUS. For nonvisible tumors, invasive methods may be a useful diagnostic tool. Malignant tumors showed reduced staining for insulin and proinsulin and increased staining for glucagon.

scholarly journals Serological studies ofBacteroides fragilis

1977 ◽  
Vol 79 (2) ◽  
pp. 233-241 ◽  
Author(s):  
K. M. Elhag ◽  
K. A. Bettelheim ◽  
Soad Tabaqchali
Keyword(s):  
Bacteroides Fragilis ◽  
Gram Negative ◽  
Biochemical Characteristics ◽  
Cross Reactions ◽  
Homologous Antigen ◽  
Specific Antisera ◽  
Serological Studies ◽  
The Cross ◽  
O Antigens

SUMMARYUsing direct agglutination methods, a simple serological scheme for the classification ofBacteroides fragilisis described. Twenty strains ofB. fragiliswere selected by a process of successive screening from 151 strains obtained from various sources. O-antigens were prepared from the 20 strains, and used to raise antisera in rabbits.Each of the 20 antisera reacted with its homologous antigen and eight antisera cross-reacted with other subspecies. These cross-reactions were successfully removed after absorption of the antisera with the cross-reacting antigens, resulting in 19 type-specific antisera, titres ranging from 40 to 320, and 19 distinct serotypes ofB. fragilis. There was no correlation between the antigenic and the biochemical characteristics of these strains and no cross-reactions occurred with other gram-negative anaerobes,B. melaninogenicus, Sphaerophorus necrophorusandFuso-bacterium necrogenes.

Diagnostic Classification of LD: A Practical Necessity and a Procedural Problem

1979 ◽  
Vol 2 (2) ◽  
pp. 56-62 ◽  
Author(s):  
Howard S. Adelman
Keyword(s):  
Learning Disabilities ◽  
Diagnostic Procedures ◽  
Diagnostic Classification ◽  
Ethical Considerations ◽  
Diagnostic Practices ◽  
Considerable Debate ◽  
Practical Necessity ◽  
Assessment Activity ◽  
Procedural Problem

As the diagnosis of learning disabilities has become widespread and commonplace, considerable debate has raged over the issue of labeling. Since the polemics have generated some confusion, it is important that professionals not lose sight of the major purposes and serious concerns related to diagnostic practices. In a two-part series, Adelman will (1) highlight why diagnostic procedures are necessary and why it is difficult to arrive at a valid diagnosis and (2) offer some perspectives on research and ethical considerations related to current LD diagnostic practices. In this first article, discussion of purposes and problems involved in diagnosing LD focuses on three topics: (a) how diagnosis relates to other assessment activity, (b) the objectives of diagnostic classification, and (c) specific conceptual concerns regarding the LD label.

scholarly journals Bromoperoxidase Producing Bacillus spp. Isolated from the Hypobranchial Glands of A Muricid Mollusc Are Capable of Tyrian Purple Precursor Biogenesis

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 264
Author(s):  
Ajit Kumar Ngangbam ◽  
Peter Mouatt ◽  
Joshua Smith ◽  
Daniel L. E. Waters ◽  
Kirsten Benkendorff
Keyword(s):  
Potential Role ◽  
Bacillus Species ◽  
Bacterial Symbionts ◽  
Bacterial Isolates ◽  
Liquid Chromatography Mass Spectrometry ◽  
Marine Agar ◽  
Tyrian Purple ◽  
Historical Importance ◽  
Bacillus Spp ◽  
Vibrio Spp

The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor to Tyrian purple stored in the Australian species, Dicathais orbita, has been proposed. This study aimed to culture bacterial symbionts from the purple producing hypobranchial gland, and screen the isolates for bromoperoxidase genes using molecular methods. The ability of bromoperoxidase positive isolates to produce the brominated indole precursor to Tyrian purple was then established by extraction of the culture, and analysis by liquid chromatography–mass spectrometry (LC–MS). In total, 32 bacterial isolates were cultured from D. orbita hypobranchial glands, using marine agar, marine agar with hypobranchial gland aqueous extracts, blood agar, thiosulphate citrate bile salts sucrose agar, and cetrimide agar at pH 7.2. These included 26 Vibrio spp., two Bacillus spp., one Phaeobacter sp., one Shewanella sp., one Halobacillus sp. and one Pseudoalteromonas sp. The two Bacillus species were the only isolates found to have coding sequences for bromoperoxidase enzymes. LC–MS analysis of the supernatant and cell pellets from the bromoperoxidase producing Bacillus spp. cultured in tryptone broth, supplemented with KBr, confirmed their ability to produce the brominated precursor to Tyrian purple, tyrindoxyl sulphate. This study supports a potential role for symbiotic Bacillus spp. in the biosynthesis of Tyrian purple.

scholarly journals Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

2008 ◽  
Vol 52 (No. 2) ◽  
pp. 49-53 ◽  
Author(s):  
N. Topic Popovic ◽  
R. Coz-Rakovac ◽  
I. Strunjak-Perovic
Keyword(s):  
Vibrio Anguillarum ◽  
Rapid Identification ◽  
Identification System ◽  
Bacterial Isolates ◽  
Yersinia Ruckeri ◽  
Fish Health ◽  
Fish Pathogens ◽  
Phenotypic Tests ◽  
Fish Bacteria ◽  
Important Fish

The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as <i>Yersinia ruckeri</i>, <i>Edwardsiella ictaluri</i>, <i>Vibrio anguillarum</i>.

Bacteria as Biocontrol Agents for Rearing Larvae of the Crab Portunus trituberculatus

1992 ◽  
Vol 49 (11) ◽  
pp. 2373-2376 ◽  
Author(s):  
Kinya Nogami ◽  
Masachika Maeda
Keyword(s):  
Bacterial Strain ◽  
Growth Stage ◽  
Larval Growth ◽  
Vibrio Anguillarum ◽  
Bacterial Density ◽  
Portunus Trituberculatus ◽  
Bacterial Populations ◽  
Crab Larvae ◽  
Bacterial Assemblages ◽  
Vibrio Spp

Bacterial strain PM-4, isolated from a crustacean culturing pond, improved the growth of crab (Portunus trituberculatus) larvae and repressed the growth of Vibrio anguillarum in seawater. PM-4 was cultured in a large quantity and was added daily for 6 d to 200 m3 of seawater used for culturing crab larvae. Initial bacterial density in the crustacean culture water was 106 cells/mL When bacteria increased to more than 107 cells/mL (at crab larval growth stage zoea II), the protozoan population, primarily flagellates, grew rapidly and reduced bacterial numbers to 106 cells/mL Among the bacterial assemblages monitored, added PM-4 dominated the bacterial populations, i.e. Vibrio spp. numbers decreased or even became undetectable in seawater. Production of crab larvae was greatly increased by adding bacterial strain PM-4 to their culture water.

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