Biochemical characteristics and genetic diversity of Vibrio spp. and Aeromonas hydrophila strains isolated from the Lac of Bizerte (Tunisia)

2010 ◽  
Vol 26 (11) ◽  
pp. 2037-2046 ◽  
Author(s):  
Snoussi Mejdi ◽  
Noumi Emira ◽  
Messaoud Ali ◽  
Hajlaoui Hafedh ◽  
Bakhrouf Amina
Keyword(s):  
Genetic Diversity ◽  
Aeromonas Hydrophila ◽  
Biochemical Characteristics ◽  
Vibrio Spp

Effect of Processing and Storing Oyster Meats on Concentrations of Indicator Bacteria, Vibrios and Aeromonas hydrophila

1984 ◽  
Vol 47 (8) ◽  
pp. 598-601 ◽  
Author(s):  
M. A. HOOD ◽  
R. M. BAKER ◽  
F. L. SINGLETON
Keyword(s):  
Aeromonas Hydrophila ◽  
Processing Plants ◽  
Commercial Processing ◽  
Processing And Storage ◽  
The Individual ◽  
Subsequent Storage ◽  
Pathogenic Vibrios ◽  
And Storage ◽  
Bacterial Groups ◽  
Vibrio Spp

Oyster meats were examined from three commercial processing plants at different stages of processing and storage for four standard indicator bacterial groups, five species of vibrios reported to be associated with shellfish associated gastroenteritis, and Aeromonas hydrophila. Processing reduced the overall microbial load, but the individual bacterial groups examined remained statistically the same throughout processing. Upon storage, the concentration of Vibrio parahaemolyticus significantly declined with a concomitant increase in levels of A. hydrophila, and the levels of all other Vibrio spp. remained statistically the same. The findings suggest that, while processing results in a cleaner appearing product, processing does not eliminate potentially pathogenic vibrios. However, processing and subsequent storage of oyster meats do not appear to increase the levels of vibrios.

Characteristics and Identification of Pasteurella and Vibrio Species Pathogenic to Fishes using API-20E (Analytab Products) Multitube Test Strips

1982 ◽  
Vol 39 (12) ◽  
pp. 1725-1729 ◽  
Author(s):  
Michael L. Kent
Keyword(s):  
Vibrio Anguillarum ◽  
Diagnostic Procedures ◽  
Blood Agar ◽  
Marine Agar ◽  
Biochemical Characteristics ◽  
Test Strips ◽  
Fish Pathology ◽  
Fish Bacteria ◽  
Vibrio Spp

Morphological, cultural, and biochemical characteristics of Pasteurella spp. and Vibrio spp. pathogenic to fishes are investigated. Vibrio anguillarum, V. ordalii, V. alginolyticus, V. parahaemolyticus, an unidentified Vibrio sp. (AN1K), Pasteurella piscicida, and P. multocida were cultured on API-20E multitube test strips (Analytab Products) and in Baumann's marine media with similar results. The API-20E Profile Index was not useful for classification of fish bacteria studied and, using the index. Vibrio sp. AN1K was misidentified as P. multocida. Bacteria studied were also grown on Marine Agar (Difco) and on blood agar at 25 and 37 °C. On blood agar at 37 °C some vibrios resembled pasteurellas as a result of pleomorphism and lack of motility. Simple diagnostic procedures for identifying Pasteurella spp. and Vibrio spp. associated with fishes using the API-20E with modifications are presented.Key words: Pasteurella, Vibrio, characteristics, Analytical Profile Index, fish pathology

scholarly journals Aeromonas hydrophilain livestock: incidence, biochemical characteristics and antibiotic susceptibility

1984 ◽  
Vol 92 (3) ◽  
pp. 365-375 ◽  
Author(s):  
S. J. Gray
Keyword(s):  
Drinking Water ◽  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Strong Positive Correlation ◽  
Individual Animal ◽  
Biochemical Characteristics ◽  
Positive Correlation ◽  
Enrichment Technique ◽  
Faecal Samples ◽  
Untreated Water

SummaryFaecal samples from 110 horses, 115 pigs, 111 sheep and 123 cows were examined for the presence ofAeromonas hydrophila, which was also sought in the available drinking water.The overall faecal rate was 11·8%, but significantly more bovine than other samples were found to be positive. There was significant association between the isolation ofA. hydrophilafrom all animal faeces and its presence in drinking water, but this was not found when individual animal groups were analysed separately. An enrichment technique increased the total number of isolates by 77·1 %. Strains of differing origins could not be differentiated by biotyping, although fermentation of sorbitol was associated with bovine isolates. There was a strong positive correlation between positive reactions for V–P, gluconate oxidase and haemolysis of rabbit erythrocytes, tests which had previously been shown to correlate with production of enterotoxin and cytotoxin. Biotypes giving positive reactions for these tests were most frequently isolated from cows, sheep and untreated water, and less frequently from pigs and horses. Most strains ofA. hydrophilawere resistant to amoxycillin, carbenicillin and cephradine, and sensitive to gentamicin, chloramphenicol and neomycin.

Virulotyping and genetic diversity of Aeromonas hydrophila isolated from Nile tilapia (Oreochromis niloticus) in aquaculture farms in Egypt

Aquaculture ◽  
2021 ◽  
pp. 736781
Author(s):  
Yasmine H. Tartor ◽  
El-Sayed Y. EL-Naenaeey ◽  
Hossam M. Abdallah ◽  
Mohamed Samir ◽  
Mayada M. Yassen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas hydrophila

2012 ◽  
Vol 38 (3) ◽  
pp. 41-50 ◽  
Author(s):  
Karol Korzekwa ◽  
Iwona Gołaś ◽  
Monika Harnisz
Keyword(s):  
Genetic Diversity ◽  
River Water ◽  
Aeromonas Hydrophila ◽  
Microbiological Quality ◽  
Anthropogenic Pollution ◽  
Electrophoretic Patterns ◽  
Quality Of Water ◽  
Water Based ◽  
Many Sources

Abstract Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX). None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.

Genetic heterogeneity amongVibrio alginolyticusstrains, and design of a PCR-based identification method usinggyrBgene sequence

2018 ◽  
Vol 64 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Supansa Bunpa ◽  
Mitsuaki Nishibuchi ◽  
Jumroensri Thawonsuwan ◽  
Natthawan Sermwittayawong
Keyword(s):  
Genetic Diversity ◽  
Chain Reaction ◽  
Marine Animals ◽  
Gyrb Sequence ◽  
Highly Sensitive ◽  
Southern Thailand ◽  
Polymerase Chain ◽  
Species Specific ◽  
Dna Template ◽  
Vibrio Spp

Vibrio alginolyticus, a pathogen among humans and marine animals, is ubiquitous in marine environments. The aims of this study were to analyze the relationships between genetic diversity and origins, and to develop new primers based on the gyrB sequence to identify V. alginolyticus isolated from various sources. To determine the genetic diversity of this bacterium, an arbitrarily primed polymerase chain reaction (AP-PCR) technique was performed on 36 strains of V. alginolyticus isolated from diarrhea patients and from diseased marine animals and environments in southern Thailand. The results showed distinct DNA fingerprints of all strains, indicating that they are genetically heterogeneous. For species-specific identification of V. alginolyticus, primers targeting the gyrB gene of V. alginolyticus were developed. Thirty reference Vibrio spp., 13 non-Vibrio spp., and 160 strains of V. alginolyticus isolated from various sources in southern Thailand were used to evaluate the specificity of these primers. Our results showed that the gyrB primers could specifically identify V. alginolyticus from all sample types. In addition, the detection limit of the PCR was at least 95 pg of DNA template. Therefore, we concluded that the newly designed gyrB primers are rapid, highly sensitive, and specific to identify V. alginolyticus isolated from various sources.

scholarly journals ISOLAMENTOS DE BACTÉRIAS E VÍRUS EM PEIXES DE ÁGUAS DO MUNICÍPIO DE SANTA MARIA E ARREDORES

1992 ◽  
Vol 22 (1) ◽  
pp. 93-100
Author(s):  
Terezinha Flores Canabarro ◽  
Rudi Weiblen ◽  
Deodoro Atlante Brandão ◽  
Dolores Spanevello
Keyword(s):  
Aeromonas Hydrophila ◽  
Edwardsiella Tarda ◽  
Plesiomonas Shigelloides ◽  
Pseudomonas Spp ◽  
Acinetobacter Spp ◽  
Santa Maria ◽  
Vibrio Spp

Bactérias e vírus de peixes de águas do município de Santa Maria e arredores foram isolados, sendo comparados com os descritos na literatura como agentes etiológicos das doenças infecto-contagiosas dos peixes. Foram examinados 160 peixes, coletados nas quatro estações do ano, em rios, açudes e tanques de piscicultura Estes peixes pertenciam à populações aparentemente sadias. Para os exames bacteriológicos foram coletadas amostras diretamente dos rins e de lesões externas e internas quando presentes, num total de 232. Os exames destas amostras resultaram no isolamento e identificação de 21 diferentes gêneros de bactérias. Das bactérias isoladas, 11 são relatadas como patogênicas para os peixes, sendo estas Aeromonas hydrophila, Plesiomonas shigelloides, Edwardsiella tarda. Flavobacterium spp., Pseudomonas spp., Vibrio spp., Pasteurella spp., Micrococcus spp., Corynebacterium spp., Acinetobacter spp., e Streptococcus spp. . Para os exames virológicos foram usadas amostras de fígado, baço, gônadas e outros órgãos que mostrassem alterações macroscópicas, num total de 255. Estes materiais foram passados frente à linha celular Epitelioma Papiloso de Carpa (EPC) e dois materiais apresentaram efeito citopatogênico. A sétima passagem destes materiais em cultivo celular foi preparada para microscopia eletrônica, sendo evidenciadas partículas virais. Estas partículas estavam localizadas no citoplasma das células e mediam aproximadamente 50nm de diâmetro.

scholarly journals Application of Metabolomic Analysis in Exploration of Plant Genetic Resources

2019 ◽  
Vol 73 (6) ◽  
pp. 494-501 ◽  
Author(s):  
Igor G. Loskutov ◽  
Tatyana V. Shelenga ◽  
Alexander V. Rodionov ◽  
Valentina I. Khoreva ◽  
Elena V. Blinova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Quality Indicators ◽  
Plant Genetic Resources ◽  
Chemical Compounds ◽  
Cereal Crops ◽  
Metabolomic Analysis ◽  
Biochemical Characteristics ◽  
Abiotic Stressors ◽  
Technological Quality

Abstract The article addresses the issues of using metabolomic analysis to study genetic resources of cereal crops in order to (1) determine phylogenetic linkages between species (the degree of domestication); (2) within species to describe genetic diversity according to its responses to biotic and abiotic stressors and biochemical characteristics (chemical compounds) determining food, feed and technological quality indicators; and (3) select the most resistant and highest-quality geno-types for complex breeding use.

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