A Conceptual Model for the Estimation of the Sensitivity of Black Fly Larvae to Bacillus thuringiensis var. israelensis
Results from bioassays of a commercial formulation of Bacillus thuringiensis var. israelensis (Teknar HP-D) are combined with an empirical model of the feeding rate of larvae of a target species of black fly (Simulium venustum/verecundum) to develop a model relating the mortality of larvae to the amount of formulation ingested per unit mass of larvae (specific ingested dose). LD50 of Teknar HP-D was about 1 μg wet mass of Teknar per milligram dry mass of larvae, and independent of size of the larvae and seston concentration. Compared with standard assay analysis relating the mortality to the dose (milligrams per litre times seconds), the proposed model allowed more precise and replicable estimation of the effective dose. Reanalysis of published data on the potency of another Bti formulation (Teknar WDC), confirmed the superiority of the proposed model over the standard procedure. Susceptibility of larvae to particulate larvicides is mainly a function of the larval feeding rate. In addition to size of the larvae, temperature, and seston concentration, analysis of the model reveals that the length of the contact period and the variability of feeding rates among individual larvae are important factors determining the mortality rate following treatments.