RELATIONSHIP OF TAXA IN THE GENUS MEDICAGO AS REVEALED BY HYBRIDIZATION. VI. M. LACINIATA × M. SAUVAGEI
Medicago laciniata (L) Mill. and M. sauvagei Nègre were intercrossed in both directions. The F1's of the cross M. sauvagei ♂ × M. laciniata ♀ were chlorophyll deficient, and F1's of the reciprocal cross were normal green, indicating cytoplasmic influence. In the F2 of chlorophyll deficient F1's, segregation for chlorophyll deficiency took place, revealing interaction of cytoplasmic factor/s with gene/s of M. laciniata. Some transmission of the cytoplasmic factor through pollen of M. sauvagei was indicated by somatic chlorophyll deficiency in sectors of a few leaves of F1's of M. laciniata ♂ × M. sauvagei ♀. The serrate margin leaf character of M. sauvagei and the apical coil spininess of M. laciniata were transmitted as recessives. Because of the poor seed set in F1's and high seedling mortality of F2's, the number of genes determining these characters is not certain. The two taxa are considered separate species because of low interfertility; their close phylogenetic relationship, however, is indicated by normal meiosis in F1's and by hybridization.