RELATIONSHIP OF TAXA IN THE GENUS MEDICAGO AS REVEALED BY HYBRIDIZATION. VI. M. LACINIATA × M. SAUVAGEI

1972 ◽  
Vol 14 (4) ◽  
pp. 823-828 ◽  
Author(s):  
S. M. Singh ◽  
K. Lesins

Medicago laciniata (L) Mill. and M. sauvagei Nègre were intercrossed in both directions. The F1's of the cross M. sauvagei ♂ × M. laciniata ♀ were chlorophyll deficient, and F1's of the reciprocal cross were normal green, indicating cytoplasmic influence. In the F2 of chlorophyll deficient F1's, segregation for chlorophyll deficiency took place, revealing interaction of cytoplasmic factor/s with gene/s of M. laciniata. Some transmission of the cytoplasmic factor through pollen of M. sauvagei was indicated by somatic chlorophyll deficiency in sectors of a few leaves of F1's of M. laciniata ♂ × M. sauvagei ♀. The serrate margin leaf character of M. sauvagei and the apical coil spininess of M. laciniata were transmitted as recessives. Because of the poor seed set in F1's and high seedling mortality of F2's, the number of genes determining these characters is not certain. The two taxa are considered separate species because of low interfertility; their close phylogenetic relationship, however, is indicated by normal meiosis in F1's and by hybridization.

1968 ◽  
Vol 10 (2) ◽  
pp. 263-275 ◽  
Author(s):  
K. Lesins ◽  
A. Erac

In crosses between the two taxa Medicago striata Bast, and M. littoralis Rohde a high mortality of gametes and seedlings, and sterility of some plants were noted which were not related to gross chromosomal rearrangements. Although the F1, F2 and F3 generations from reciprocal crosses differed in chlorophyll deficiencies (indicating a cytoplasmic influence) a genic cause became evident from segregations for chlorophyll characters in the F2 and F3. Transference of the cytoplasmic factor by the pollen is indicative.Segregation for pod coiling direction indicated that the character was determined by one or two genetic factors of which the clockwise coiling direction is recessive. The spininess appeared to be determined by one genetic factor, of which the spineless allele is recessive.On the basis of genetic differences (especially on the built-in repulsion systems for normal chlorophyll development of opposite species) the two taxa should be considered two different species.


1971 ◽  
Vol 13 (2) ◽  
pp. 335-346 ◽  
Author(s):  
K. Lesins ◽  
S. M. Singh ◽  
A. Erac

Urban (1873) characterized Medicago L. division Intertextae Urb. as having black seeds. An exception to this was found among M. granadensis Willd.; there were accessions with black as well as with brown seeds. Black seed color was dominant over brown and segregated in F2 in ratio 3:1. Two other M. granadensis marker characters: colored vs. green stems, hairy stems vs. smooth stems similarly segregated in a 3:1 ratio, the first named characters being dominant. The colored and white patch in the middle of leaflets appeared to be determined by a gene P for patching, a gene C being responsible for the presence of color. No recombinations were found between colored patch in leaflets, colored stems, and black seeds, indicating that there is only one gene for color with pleiotropic action, or that the genes are closely linked M. granadensis did not hybridize with M. intertexta and M. ciliaris. It clearly is a separate species.M. intertexta Mill., M. ciliaris All. and M. muricoleptis Tin. could be intercrossed. Marker characters, red basal patch vs. no patch in leaflets and hairs vs. no hairs on pods, segregated in F2 in a normal 3:1 ratio, the first named characters being dominant, Segregation did not indicate that there was any linkage between the two characters. Seed weight difference was determined by two genes acting in an additive manner. Distribution of leaf marking in seed weight classes indicated an independent segregation. Pollen fertility of F1's was less than 50%. There were observed irregularities in 30% of meiotic metaphases studied in F1 of M. muricoleptis × M. ciliaris. Because of some interbreeding barrier the taxa may be considered separate species though their hereditary material can be interchanged.


2006 ◽  
Vol 84 (2) ◽  
pp. 243-253 ◽  
Author(s):  
Wendy A. Untereiner ◽  
Françoise A. Naveau ◽  
Jason Bachewich ◽  
Andrea Angus

During an investigation of lignicolous ascomycetes from Luxembourg, we isolated a Phialophora -like species that produced striking red colonies. To confirm the identity of this fungus as Catenulifera rhodogena , we compared it with isolates of Catenulifera rhodogena and Hyphodiscus hymeniophilus on a variety of media. Portions of the β-tubulin gene and the nuclear ribosomal DNA cistron (internal transcribed spacers (ITS) and large subunit (LSU)) were sequenced to examine the relationship of isolates of Catenulifera rhodogena and Hyphodiscus hymeniophilus from different substrates and to test the hypothesis that Cadophora and Catenulifera are congeneric. The phylogenetic position of Catenulifera within the Ascomycota was investigated based on the analysis of the small-subunit (SSU) rDNA sequences. The isolates examined were indistinguishable micromorphologically and closely related phylogenetically. Three strains of Catenulifera rhodogena from bark or wood and one strain from Piptoporus betulinus formed a strongly supported clade in analyses of β-tubulin and ITS sequences. This clade did not encompass the ex-type isolates of Cistella rubescens and Scopulariopsis rhodogena or a second isolate of Catenulifera rhodogena from Piptoporus betulinus. Analysis of partial LSU sequences confirmed the close phylogenetic relationship of Catenulifera rhodogena and Hyphodiscus hymeniophilus but provided no evidence that the isolates could be grouped by substrate or that Catenulifera is synonymous with Cadophora. The position of Catenulifera within the Helotiales was not resolved based on the comparison of LSU and SSU sequences, but the isolate for which we obtained complete SSU sequence grouped with the root endophyte Phialocephala fortinii. Comparison of ITS sequences confirmed the close phylogenetic relationship of Hyphodiscus to members of the Dermateaceae and Hyaloscyphaceae.


1982 ◽  
Vol 131 (3) ◽  
pp. 287-290 ◽  
Author(s):  
Elke Seewaldt ◽  
Karl-Heinz Schleifer ◽  
Eberhard Bock ◽  
Erko Stackebrandt

2019 ◽  
Author(s):  
Adriano de Bernardi Schneider ◽  
Denis Jacob Machado ◽  
Daniel Janies

The ongoing and severe public health threat of viruses of the family Flaviviridae, including dengue, hepatitis C, West Nile, yellow fever, and zika, demand a greater understanding of how these viruses evolve, emerge and spread in order to respond. Central to this understanding is an updated phylogeny of the entire family. Unfortunately, most cladograms of Flaviviridae focus on specific lineages, ignore outgroups, and rely on midpoint rooting, hampering their ability to test ingroup monophyly and estimate ingroup relationships. This problem is partly due to the lack of fully annotated genomes of Flaviviridae, which has genera with slightly different gene content, hindering genome analysis without partitioning. To tackle these problems, we developed an annotation pipeline for Flaviviridae that uses a combination of ab initio and homology-based strategies. The pipeline recovered 100% of the genes in reference genomes and annotated over 97% of the expected genes in the remaining non curated sequences. We further demonstrate that the combined analysis of genomes of all genera of Flaviviridae (Flavivirus, Hepacivirus, Pegivirus, and Pestivirus), as made possible by our annotation strategy, enhances the phylogenetic analyses of these viruses for all optimality criteria that we tested (parsimony, maximum likelihood, and posterior probability). The final tree sheds light on the phylogenetic relationship of viruses that are divergent from most Flaviviridae and should be reclassified, especially the soybean cyst nematode virus 5 (SbCNV-5) and the Tamana bat virus. We also corroborate the close phylogenetic relationship of dengue and zika viruses with an unprecedented degree of support.


BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Yanni Dong ◽  
Saurabh Gupta ◽  
Rixta Sievers ◽  
Jason J. Wargent ◽  
David Wheeler ◽  
...  

Abstract Background Pachycladon cheesemanii is a close relative of Arabidopsis thaliana and is an allotetraploid perennial herb which is widespread in the South Island of New Zealand. It grows at altitudes of up to 1000 m where it is subject to relatively high levels of ultraviolet (UV)-B radiation. To gain first insights into how Pachycladon copes with UV-B stress, we sequenced its genome and compared the UV-B tolerance of two Pachycladon accessions with those of two A. thaliana accessions from different altitudes. Results A high-quality draft genome of P. cheesemanii was assembled with a high percentage of conserved single-copy plant orthologs. Synteny analysis with genomes from other species of the Brassicaceae family found a close phylogenetic relationship of P. cheesemanii with Boechera stricta from Brassicaceae lineage I. While UV-B radiation caused a greater growth reduction in the A. thaliana accessions than in the P. cheesemanii accessions, growth was not reduced in one P. cheesemanii accession. The homologues of A. thaliana UV-B radiation response genes were duplicated in P. cheesemanii, and an expression analysis of those genes indicated that the tolerance mechanism in P. cheesemanii appears to differ from that in A. thaliana. Conclusion Although the P. cheesemanii genome shows close similarity with that of A. thaliana, it appears to have evolved novel strategies allowing the plant to tolerate relatively high UV-B radiation.


Zootaxa ◽  
2012 ◽  
Vol 3570 (1) ◽  
pp. 82 ◽  
Author(s):  
M. GUADALUPE DEL RÍO ◽  
ANALIA A. LANTERI

The monotypic genus Curiades Pascoe 1880 (Curculionidae: Entiminae: Naupactini) is redescribed based on the type species C. boisduvali (Boheman 1840), endemic to Río de Janeiro, Brazil. Curiades boisduvali shows a characteristic color pattern and vestiture composed of long erect setae that resembles species of Dasymutilla Ashmead 1899, a genus of Mutillidae (Hymenoptera) whose wingless females have aposematic colors and painful stings. The similarities between the wasp (model) and the weevil (mimic) suggest a case of Batesian mimicry. The morphological characters of the rostrum and antennae justify a close phylogenetic relationship of Curiades and Platyomus Sahlberg 1823, even though based on its hairy vestiture the former is superficially more similar to Trichaptus Pascoe 1880, another Brazilian naupactine mimic of mutillid wasps. The present contribution includes a redescription of Curiades and its only known species, accompanied by habitus photographs, line drawings of genitalia and other diagnostic features.


2017 ◽  
Vol 49 (5) ◽  
pp. 467-481 ◽  
Author(s):  
Robert LÜCKING ◽  
R. Greg THORN ◽  
Irja SAAR ◽  
Michele D. PIERCEY-NORMORE ◽  
Bibiana MONCADA ◽  
...  

AbstractMolecular studies have shown the type collection of Omphalina oreades to be conspecific with a small brown basidiolichen from the Appalachian range in Newfoundland, both with 4-spored basidia. Two sequences deposited in GenBank, originally identified as O. grisella, fell in the same clade. Sequences of the type collection of Omphalia grisella, with 2-spored basidia, formed a sister clade together with two GenBank deposits, one identified as O. grisella and the other as Omphalina velutina. Omphalina oreades is recombined here as Lichenomphalia oreades comb. nov., and the species redescribed and illustrated. Sequences of the internal transcribed spacer regions of nuclear ribosomal DNA (ITS rDNA) from the algae associated with two collections of L. oreades fell within a highly supported clade with members of an undetermined species of Coccomyxa. The most abundant algal ribosomal large subunit sequence from granules at the base of a different collection matched GenBank sequences identified as Chloroidium ellipsoideum, which is probably either a free-living algal species or a partner to a species of Trapeliopsis. The second most abundant sequence matched Coccomyxa subellipsoidea and is the most likely photobiont of L. oreades. Further studies are required to elucidate the relationship of L. velutina to these taxa.


1972 ◽  
Vol 104 (3) ◽  
pp. 361-388 ◽  
Author(s):  
Gerald N. Lanier

AbstractThe taxonomic concepts of the Ips species of groups IV and X were tested by controlled breeding, bioassay of sex pheromone specificity, and examination of morphological and karyological details. No changes were made in the status of Ips pini, I. bonanseai, and I. avulsus of group IV. I. interstitialis of group X is removed from synonymy with I. calligraphus, and I. ponderosae, also previously considered a synonym of I. calligraphus, is assigned subspecific rank. Assertions of close phylogenetic relationship of the Eurasian I. duplicatus and I. sexdentatus with groups IV and X, respectively, are rejected.I. pini from widely separated localities were interfertile although egg hatchability was subnormal in some crosses involving F1 from matings of beetles from western and eastern North America. I. pini and I. bonanseai readily mated but less than 3% of the eggs hatched and the larvae died. There was no evidence of breeding incompatibility in crosses of I. c. calligraphus from Florida and California or I. c. ponderosae from New Mexico and South Dakota. However, hatchability differed drastically between reciprocal pairings of the I. calligraphus subspecies, possibly as a result of cytoplasmic incompatibility. All eggs produced in pairings of I. interstitialis with either of the I. calligraphus subspecies failed to hatch.First meiotic metaphase of I. pini, I. bonanseai, and both I. calligraphus subspecies invariably showed the formula 15AA + Xyp. This karyotypic formula also applied to I. avulsus except for a few individuals that had 2 small supernumerary chromosomes. The karyotype of I. interstitialis was not determined. Heteromorphic bivalents and univalents were detected in hybrids of the I. calligraphus subspecies.Cross attractiveness to sex pheromones was demonstrated for I. pini from different regions, for I. pini and I. bonanseai, and for the I. calligraphus subspecies. When samples of attractive frass from eastern and western I. pini males were presented simultaneously, eastern females responded equally to both samples but western females favored western frass by a margin of 2 to 1. Similarly, I. c. calligraphus clearly discriminated in favor of consubspecific pheromone while I. c. ponderosae did not.The locality of several of Eichhoff’s holotypes is noted.


Diversity ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Vlatka Mičetić Stanković ◽  
Branka Bruvo Mađarić ◽  
Mladen Kučinić

Although freshwater habitats, especially springs, are widely recognized as top-priority habitats for monitoring and conservation procedures, their fauna, especially water beetles, are still poorly studied in the southeastern part of Europe. Thus, the dominant water beetle in these habitats, E. bosnica (Zaitzev, 1908) (Insecta: Coleoptera: Elmidae), has been completely ignored and misidentified. This study represents the first review of its taxonomy and its population and ecological traits. Both published and unpublished data are presented and discussed, as well as the results of field sampling in 46 springs and other waterbodies conducted in this region from 2004 to 2019. The identification characters of the male genitalia and the first DNA barcode of the species are presented. The results confirm the close phylogenetic relationship of E. bosnica with E. aenea (Müller, 1806) and E. rioloides (Kuwert, 1890). The species proved to be a useful environmental descriptor and can easily be used as a biological indicator due to its easy identification. The species shows remarkable sensitivity to environmental conditions and inhabits sites that are potentially under increased anthropogenic pressure and could disappear at an alarming rate. Thus, karstic habitats should be included in future conservation and monitoring procedures in this part of Europe.


Sign in / Sign up

Export Citation Format

Share Document