Rye heterochromatin. II. Characterization of a derivative from chromosome 1DS.1RL with a reduced amount of the major repeating sequence

1986 ◽  
Vol 28 (5) ◽  
pp. 658-664 ◽  
Author(s):  
R. M. D. Koebner ◽  
R. Appels ◽  
K. W. Shepherd

Homoeologous recombination between wheat chromosomes and the long arm of chromosome 1R of rye present in a wheat background as the wheat–rye translocation, 1DS.1RL, has been reported. During the course of this study an unexpected plant (308-17), with a recombinant phenotype, arose from a control population where homoeologous pairing was thought to be suppressed. The putative recombinant chromosome in plant 308-17 carried two seed protein loci located on 1RL (namely, the rye Glu-R1 locus and a recently discovered globulin-like marker) but appeared to lack C-banded heterochromatin. Further investigation of this apparent recombinant chromosome in progeny of 308-17 using a cloned probe for the 350 family of rye heterochromatic sequence indicated that some terminal heterochromatin was still present but in a much reduced amount. Because it appears that only the terminal region has been changed, the modified chromosome in 308-17 most likely did not arise from homoeologous recombination but rather from some form of heterochromatin loss event, possibly involving an unequal sister chromatid exchange within the terminal heterochromatin of 1RL. Sister lines to plant 308-17 carrying Glu-R1 contained a normal amount of 1RL heterochromatin.Key words: rye–wheat, heterochromatin, recombinant, unequal sister chromatid exhange.






2020 ◽  
Vol 95 ◽  
pp. 103075
Author(s):  
Fabiola Sánchez-López ◽  
Víctor J. Robles-Olvera ◽  
Madeleine Hidalgo-Morales ◽  
Apollinaire Tsopmo


2009 ◽  
Vol 84 (6) ◽  
pp. 407-416 ◽  
Author(s):  
Yadav Prasad Gyawali ◽  
Shuhei Nasuda ◽  
Takashi R. Endo


1975 ◽  
Vol 26 (3) ◽  
pp. 467 ◽  
Author(s):  
PJ Robinson ◽  
RG Megarrity

Seed protein patterns of 182 Stylosanthes accessions, representing 16 species and two hybrids, were obtained by polyacrylamide gel electrophoresis of crude extracts. All species could be recognized by examination of photographs and densitometer traces of the gels. Within the species capitata, guyanensis, hamata and viscosa considerable variation occurred, whilst the variation in humilis, scabra and fruticosa was not as great. Data from the densitometer traces were analysed by various methods of pattern analysis and the resulting classifications compared. A variance-standardized Euclidean distance coefficient was found to be the similarity measure of choice, whilst selection of fusion strategy was not as critical.Species relationships obtained by using the chemical data were not in agreement with the accepted taxonomic division of the genus into the sections Styposanthes and Stylosanthes. A classification based on the complete data set was compared with a working classification based on morphological and agronomic data, which is used in the agronomic assessment of the genus. Only within S. scabra did the two classifications conform. Morphological–agronomic (M–A) types within the species hamata and subsericea could be distinguished by the examination of the fine structure of the densitometer traces, whilst groups based on protein data in the species ahumilis, guyanensis, fruticosa and viscosa did not correspond with M–A groups. The application of seed protein patterns as a rapid and inexpensive means of identifying introductions of the genus at the species level, as well as characterizing types within certain species, is proposed.



1977 ◽  
Vol 28 (2) ◽  
pp. 223 ◽  
Author(s):  
R Shorter ◽  
DE Byth ◽  
VE Mungomery

A pattern analysis approach, based on classification and ordination, is presented for the characterization of environmental contributions to differences among lines in mean performance and response across environments. Other approaches to the analysis of line performance and environmental interactions are also considered. A population of soybean lines is used to illustrate the analyses for two characters, seed yield and seed protein percentage. In general, correlation of line performance over environments indicated that only moderate similarity of relative line performance existed over years or locations. These associations did not provide a clear basis for rationalization of test sites. Partitioning of variance components allowed a general strategy for sampling environments to be defined. Marked differences existed among environments for their contribution to environmental interaction, and these generally were consistent among locations for the two years of testing. Classificatory and ordination analyses were applied separately, and the contributions of each of the test environments were determined. These procedures confirmed that large differences in line response existed among environments, and provided additional and complementary information about the contributions of particular test environments to those differences. The effect of abandoning particular test sites on the recognition of differences among lines was examined by pattern analysis. The implications of the information gained through the pattern analysis approach in the development of testing strategies, and in the selection of test environments for specific objectives, are discussed.



1982 ◽  
Vol 4 (2) ◽  
pp. 135-142 ◽  
Author(s):  
G. Speit ◽  
W. Vogel ◽  
M. Wolf


1982 ◽  
Vol 30 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Jean Claude Pernollet ◽  
Su Il Kim ◽  
Jacques Mosse


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