Characterization of Stylosanthes introductions by using seed protein patterns

1975 ◽  
Vol 26 (3) ◽  
pp. 467 ◽  
Author(s):  
PJ Robinson ◽  
RG Megarrity
Keyword(s):  
Euclidean Distance ◽  
Seed Protein ◽  
Pattern Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Species Level ◽  
Protein Patterns ◽  
Data Set ◽  
Distance Coefficient ◽  
Selection Of

Seed protein patterns of 182 Stylosanthes accessions, representing 16 species and two hybrids, were obtained by polyacrylamide gel electrophoresis of crude extracts. All species could be recognized by examination of photographs and densitometer traces of the gels. Within the species capitata, guyanensis, hamata and viscosa considerable variation occurred, whilst the variation in humilis, scabra and fruticosa was not as great. Data from the densitometer traces were analysed by various methods of pattern analysis and the resulting classifications compared. A variance-standardized Euclidean distance coefficient was found to be the similarity measure of choice, whilst selection of fusion strategy was not as critical.Species relationships obtained by using the chemical data were not in agreement with the accepted taxonomic division of the genus into the sections Styposanthes and Stylosanthes. A classification based on the complete data set was compared with a working classification based on morphological and agronomic data, which is used in the agronomic assessment of the genus. Only within S. scabra did the two classifications conform. Morphological–agronomic (M–A) types within the species hamata and subsericea could be distinguished by the examination of the fine structure of the densitometer traces, whilst groups based on protein data in the species ahumilis, guyanensis, fruticosa and viscosa did not correspond with M–A groups. The application of seed protein patterns as a rapid and inexpensive means of identifying introductions of the genus at the species level, as well as characterizing types within certain species, is proposed.

Genotype × environment interactions and environmental adaptation. II. Assessment of environmental contributions

1977 ◽  
Vol 28 (2) ◽  
pp. 223 ◽  
Author(s):  
R Shorter ◽  
DE Byth ◽  
VE Mungomery
Keyword(s):  
Variance Components ◽  
Seed Protein ◽  
Pattern Analysis ◽  
Analysis Approach ◽  
General Strategy ◽  
Testing Strategies ◽  
Environmental Interactions ◽  
Relative Line ◽  
Selection Of

A pattern analysis approach, based on classification and ordination, is presented for the characterization of environmental contributions to differences among lines in mean performance and response across environments. Other approaches to the analysis of line performance and environmental interactions are also considered. A population of soybean lines is used to illustrate the analyses for two characters, seed yield and seed protein percentage. In general, correlation of line performance over environments indicated that only moderate similarity of relative line performance existed over years or locations. These associations did not provide a clear basis for rationalization of test sites. Partitioning of variance components allowed a general strategy for sampling environments to be defined. Marked differences existed among environments for their contribution to environmental interaction, and these generally were consistent among locations for the two years of testing. Classificatory and ordination analyses were applied separately, and the contributions of each of the test environments were determined. These procedures confirmed that large differences in line response existed among environments, and provided additional and complementary information about the contributions of particular test environments to those differences. The effect of abandoning particular test sites on the recognition of differences among lines was examined by pattern analysis. The implications of the information gained through the pattern analysis approach in the development of testing strategies, and in the selection of test environments for specific objectives, are discussed.

scholarly journals Molecular Cavity Topological Representation for Pattern Analysis: A NLP Analogy-Based Word2Vec Method

2019 ◽  
Vol 20 (23) ◽  
pp. 6019 ◽  
Author(s):  
Dongliang Guo ◽  
Qiaoqiao Wang ◽  
Meng Liang ◽  
Wei Liu ◽  
Junlan Nie
Keyword(s):  
Molecular Dynamics ◽  
Language Processing ◽  
Pattern Analysis ◽  
Real Data ◽  
Molecular Function ◽  
Topological Representation ◽  
Data Set ◽  
Novel Method ◽  
Topological Characteristics

Cavity analysis in molecular dynamics is important for understanding molecular function. However, analyzing the dynamic pattern of molecular cavities remains a difficult task. In this paper, we propose a novel method to topologically represent molecular cavities by vectorization. First, a characterization of cavities is established through Word2Vec model, based on an analogy between the cavities and natural language processing (NLP) terms. Then, we use some techniques such as dimension reduction and clustering to conduct an exploratory analysis of the vectorized molecular cavity. On a real data set, we demonstrate that our approach is applicable to maintain the topological characteristics of the cavity and can find the change patterns from a large number of cavities.

scholarly journals An update on multivariate return periods in hydrology

2016 ◽  
Vol 373 ◽  
pp. 175-178 ◽  
Author(s):  
Benedikt Gräler ◽  
Andrea Petroselli ◽  
Salvatore Grimaldi ◽  
Bernard De Baets ◽  
Niko Verhoest
Keyword(s):  
Time Series ◽  
Return Period ◽  
Simulated Data ◽  
Time Span ◽  
Return Periods ◽  
Data Set ◽  
Univariate Case ◽  
Definition Of ◽  
Selection Of

Abstract. Many hydrological studies are devoted to the identification of events that are expected to occur on average within a certain time span. While this topic is well established in the univariate case, recent advances focus on a multivariate characterization of events based on copulas. Following a previous study, we show how the definition of the survival Kendall return period fits into the set of multivariate return periods.Moreover, we preliminary investigate the ability of the multivariate return period definitions to select maximal events from a time series. Starting from a rich simulated data set, we show how similar the selection of events from a data set is. It can be deduced from the study and theoretically underpinned that the strength of correlation in the sample influences the differences between the selection of maximal events.

The comparison of seed protein patterns within the genusArachis by polyacrylamide gel electrophoresis

1983 ◽  
Vol 25 (4) ◽  
pp. 266-273 ◽  
Author(s):  
Eva Klozová ◽  
Jiřina Švachulová ◽  
J. Smartt ◽  
E. Hadač ◽  
Věra Turková ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Patterns

scholarly journals Differentiation and Characterization of Enteroviruses by Computer-Assisted Viral Protein Fingerprinting

1998 ◽  
Vol 36 (6) ◽  
pp. 1588-1594
Author(s):  
Diane T. Holland ◽  
Jill Senne ◽  
C. R. Peter ◽  
Connie Urmeneta ◽  
J. D. Connor
Keyword(s):  
Viral Protein ◽  
Gel Electrophoresis ◽  
Protein Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Quantitative Measure ◽  
Computer Assisted ◽  
Protein Patterns ◽  
Protein Fingerprinting ◽  
Viral Capsid Proteins

We have developed and standardized a computerized method for the typing and characterization of enteroviruses with radiolabeled viral protein fingerprints. Enteroviral proteins were radiolabeled with [35S]methionine during growth in cell culture and were then separated by polyacrylamide gel electrophoresis. The dried gel was scanned, and from the resulting computer image (which resembled an autoradiogram) protein patterns were computer extracted and stored in a database. The enterovirus database contained community and prototype strains belonging to 20 different enteroviral serotypes. Each serotype has a discrete protein pattern, and the most important pattern differences for determining each type are in the region of the viral capsid proteins VP1, VP2, and VP3. When the database was challenged with 148 clinical enterovirus strains, 144 (97%) were correctly identified by using the correlation coefficient as a quantitative measure of relatedness between two patterns. This method can identify a type in a single test and represents a practical alternative to virus neutralization because it is less expensive, is much faster (3 rather than 10 days), and does not rely on any virus-specific reagents. The results also show that most of the strains currently isolated from the community have protein patterns different from those of their older prototype strains. Viral protein fingerprinting is an evolving, dynamic system for the typing and characterization of enteroviruses. The method is appropriate for use in clinical virology and reference laboratories for the typing of enteroviruses, for the study of the epidemiology of enteroviruses, and for surveillance of enteroviruses.

scholarly journals DNA Fingerprinting and Genetic Diversity Assessment of GM Cotton Genotypes for Protection of Plant Breeder Rights

2021 ◽  
Vol 25 (04) ◽  
pp. 768-776
Author(s):  
Shakra Jamil
Keyword(s):  
Genetic Diversity ◽  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Core Set ◽  
Diversity Assessment ◽  
Cotton Genotypes ◽  
Diversity Studies ◽  
Selection Of

DNA fingerprinting is rapid, easy, and efficient method for discrimination, identification and characterization of various genotypes for protection of plant breeder’s rights (PBRs). Present study was designed for DNA fingerprinting and genetic diversity assessment of 25 GM cotton genotypes (possessing Cry1Ac gene) using 297 SSR markers through conventional PCR and Polyacrylamide gel electrophoresis. Out of 297 SSR markers, 25 markers were not amplified, 28 were monomorphic and 244 were polymorphic. A total of 1537 alleles were amplified among which 1294 (84.18%) were polymorphic. PIC value in our study ranged from 0.08 to 0.93 with an average of 0.73. Unique allelic pattern was observed for nineteen genotypes whereas six genotypes were identified using two-step identification methods. The UPGMA dendrogram divided the genotypes into two distinct clusters. Cluster I was comprised of 20 genotypes whereas cluster II was comprised of four genotypes. MNH-1020 did not obey any clustering and remained separated. The results of the structure analysis were complementary to cluster analysis and the population was divided into two subgroups. Our results evidenced narrow genetic base of the cotton genotypes cultivated in Punjab Pakistan due to use of common parents in the pedigree/parentage. Further, we proposed a core set of markers for future DNA fingerprinting and genetic diversity studies. The information generated in this study will be helpful in variety registration and subsequent protection under PBRs. Further our findings will be useful in selection of SSR markers for future studies which are focused on DNA fingerprinting and genetic diversity assessment. © 2021 Friends Science Publishers

scholarly journals The Beginning of Western Greek Amphorae Production in Western Sicily: Archaeometric and Archaeological Studies on 6th–5th Centuries BCE Amphorae Manufactured in Himera

Minerals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 762
Author(s):  
Giuseppe Montana ◽  
Luciana Randazzo ◽  
Babette Bechtold
Keyword(s):  
Special Focus ◽  
Data Set ◽  
Reflected Light ◽  
Icp Ms ◽  
Economic Interaction ◽  
Western Sicily ◽  
Thin Section Petrography ◽  
Selection Of

About 560 western Greek amphorae (6th–5th centuries BCE) re-used in enchytrismos burials were unearthed in the necropolis of the Dorian-Chalcidian colony of Himera in northwestern Sicily. Among the most striking issues is the determination of their geographical provenance. For this purpose, ceramic samples chipped from freshly broken surfaces of all the amphorae were first subdivided into macrofabrics by the use of a hand lens. Thereon, the samples were studied in accordance with standardized methods by the use of reflected light microscopy. Due to the special focus of our project on the characterization of Sicilian productions, a selection of amphorae which showed visible, macroscopic affinities with the majority of the macrofabrics previously attributed to the region of Himera was submitted for thin-section petrography at the polarizing microscope and chemical analyses (ICP-MS and ICP/OES). This new data set was compared with reference samples investigated by previous research, referring to ceramic raw clays of the colony’s territory and local tablewares of the Iato K480-type. Our study confirms the local manufacture of the entire selection of transport vessels. The identification of a production of western Greek wine (?) amphorae in Himera dating mainly from the third quarter of the 6th to the first quarter of the 5th century BCE breaks new grounds in view of a better interpretation of the colony’s economic development during the later archaic period. Furthermore, it underlines Himera’s prominent position within the wider frame of regional economic interaction.

Applications of Transmission Electron Microscopy in the Characterization of Metal Machinability

1968 ◽  
Vol 26 ◽  
pp. 442-443 ◽  
Author(s):  
L.E. Murr ◽  
A.B. Draper
Keyword(s):  
Electron Microscopy ◽  
State Physics ◽  
Test Material ◽  
Milling Machine ◽  
Rotary Table ◽  
Solid State Physics ◽  
Materials Properties ◽  
Transmission Electron ◽  
Selection Of

The industrial characterization of the machinability of metals and alloys has always been a very arbitrarily defined property, subject to the selection of various reference or test materials; and the adoption of rather naive and misleading interpretations and standards. However, it seems reasonable to assume that with the present state of knowledge of materials properties, and the current theories of solid state physics, more basic guidelines for machinability characterization might be established on the basis of the residual machined microstructures. This approach was originally pursued by Draper; and our presentation here will simply reflect an exposition and extension of this research.The technique consists initially in the production of machined chips of a desired test material on a horizontal milling machine with the workpiece (specimen) mounted on a rotary table vice. A single cut of a specified depth is taken from the workpiece (0.25 in. wide) each at a new tool location.

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

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