scholarly journals Genome-wide identification and analysis of the basic leucine zipper (bZIP) transcription factor gene family in Ustilaginoidea virens

Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1051-1059 ◽  
Author(s):  
Weixiao Yin ◽  
Peng Cui ◽  
Wei Wei ◽  
Yang Lin ◽  
Chaoxi Luo
Keyword(s):  
Transcription Factor ◽  
Phylogenetic Relationships ◽  
Leucine Zipper ◽  
Expression Profiles ◽  
Pathogenic Fungi ◽  
Bzip Transcription Factor ◽  
Basic Leucine Zipper ◽  
Ustilaginoidea Virens ◽  
Infection Period ◽  
Genome Wide

The basic leucine zipper (bZIP) transcription factor (TF) family is one of the largest and most diverse TF families widely distributed across the eukaryotes. The bZIP TF family plays an important role in growth, development, and response to abiotic or biotic stresses, which have been well characterized in plants, but not in plant pathogenic fungi. In this study, we performed genome-wide and systematic bioinformatics analysis of bZIP genes in the fungus Ustilaginoidea virens, the causal agent of rice false smut disease. We identified 28 bZIP family members in the U. virens genome by searching for the bZIP domain in predicted genes. The gene structures, motifs, and phylogenetic relationships were analyzed for bZIP genes in U. virens (UvbZIP). Together with bZIP proteins from two other fungi, the bZIP genes can be divided into eight groups according to their phylogenetic relationships. Based on RNA-Seq data, the expression profiles of UvbZIP genes at different infection stages were evaluated. Results showed that 17 UvbZIP genes were up-regulated during the infection period. Furthermore, 11 infection-related UvbZIP genes were investigated under H2O2 stress and the expression level of eight genes were changed, which confirmed their role in stress tolerance and pathogenicity. In summary, our genome-wide systematic characterization and expression analysis of UvbZIP genes provided insight into the molecular function of these genes in U. virens and provides a reference for other pathogens.

Genome-wide systematic characterization and its regulatory expression reprogramming process of the bZIP transcription factors during trauma response in Camellia sinensis

2018 ◽  
Vol 48 (11) ◽  
pp. 1279-1291
Author(s):  
Yajie Xue ◽  
Zaibao Zhang ◽  
Lei Wang ◽  
Yajun Yu ◽  
Jinbin Xiao ◽  
...  
Keyword(s):  
Phylogenetic Relationship ◽  
Camellia Sinensis ◽  
Leucine Zipper ◽  
Expression Profiles ◽  
Functional Characterization ◽  
Gene Expression Profiles ◽  
Bzip Transcription Factor ◽  
Motif Analysis ◽  
Basic Leucine Zipper ◽  
Tea Tree

Basic leucine zipper (bZIP) transcription factor (TF) genes regulate numerous biological processes, as well as biotic and abiotic responses. Although the genome of the tea tree (Camellia sinensis (L.) Kuntze) has been released, knowledge regarding the bZIP TF family in C. sinensis, e.g., phylogenetic relationship and transcriptional gene expression profiles, remains limited. In this study, we characterized 77 bZIP genes in C. sinensis based on transcriptomic and genomic data and divided them into 11 groups according to their phylogenetic relationship with those in Arabidopsis, which allowed us to identify 14 pairs of orthologous proteins shared by Arabidopsis and C. sinensis and 19 pairs of paralogous proteins in C. sinensis. Conserved motif analysis of CsbZIP proteins showed high group specificity. Our classification was supported by the predicted specificities based on DNA-binding domains, as well as the dimerization property based on characteristic features in the basic and hinge regions and the leucine zipper. Specifically, they indicated that some highly conserved amino acid residues exist across each major group in the tree of land plant life. Expression profiling analyses indicate that the CsbZIP genes are likely involved in response to trauma, and a model was established to display the unique expression of each group during different time intervals after wounding. This work provides useful clues for further functional characterization of the CsbZIP TFs.

scholarly journals Identification and characterization of the bZIP transcription factor family in yellowhorn

2020 ◽  
Vol 32 (1) ◽  
pp. 273-284 ◽  
Author(s):  
Qiaoying Chang ◽  
Xin Lu ◽  
Zhi Liu ◽  
Zhimin Zheng ◽  
Song Yu
Keyword(s):  
Transcription Factor ◽  
Leucine Zipper ◽  
Molecular Mechanisms ◽  
Bzip Transcription Factor ◽  
Biological Functions ◽  
Transcription Factor Family ◽  
Promoter Regions ◽  
Basic Leucine Zipper ◽  
Biotic Stresses ◽  
Bzip Transcription Factor Family

AbstractThe basic leucine zipper (bZIP) transcription factor family is one of the largest and most diverse families in plants, regulating plant growth and development and playing an essential role in response to abiotic and biotic stresses. However, little is known about the biological functions of bZIP proteins in yellowhorn (Xanthoceras sorbifolium). Recently, 64 XsbZIP genes were identified in the yellowhorn genome and found to be disproportionately distributed in linkage groups. The XsbZIP proteins clustered into 11 groups based on their phylogenetic relationships with AtbZIP, ZmbZIP and GmbZIP proteins. Five intron patterns in the basic and hinge regions and additional conserved motifs were defined, both supporting the group classification and possibly contributing to their functional diversity. Compared to tandem duplication, the segment duplication greatly contributed to the expansion of yellowhorn bZIP genes. In addition, most XsbZIP genes harbor several stress responsive cis-elements in their promoter regions. Moreover, the RNA-seq and qRT-PCR data indicated XsbZIP genes were extensively involved in response to various stresses, including salt (NaCl), cold and abscisic acid, with possibly different molecular mechanisms. These results provide a new understanding of the biological functions of bZIP transcription factors in yellowhorn.

scholarly journals The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Jian-Ping An ◽  
Feng-Jia Qu ◽  
Ji-Fang Yao ◽  
Xiao-Na Wang ◽  
Chun-Xiang You ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nitrate Reductase ◽  
Leucine Zipper ◽  
Anthocyanin Biosynthesis ◽  
Nitrate Assimilation ◽  
Bzip Transcription Factor ◽  
Anthocyanin Accumulation ◽  
Mobility Shift ◽  
Basic Leucine Zipper ◽  
Transgenic Apple

Abstract The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs, respectively. Furthermore, we obtained transgenic apple calli that overexpressed the MdHY5 gene, and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis genes. In addition, the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were detected. In association with increased nitrate reductase activities and nitrate contents, the results indicated that MdHY5 might be an important regulator in nutrient assimilation. Taken together, these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.

scholarly journals The Basic Leucine Zipper Transcription Factor Moatf1 Mediates Oxidative Stress Responses and Is Necessary for Full Virulence of the Rice Blast Fungus Magnaporthe oryzae

2010 ◽  
Vol 23 (8) ◽  
pp. 1053-1068 ◽  
Author(s):  
Min Guo ◽  
Wang Guo ◽  
Yue Chen ◽  
Suomeng Dong ◽  
Xing Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Magnaporthe Oryzae ◽  
Stress Responses ◽  
Rice Blast ◽  
Leucine Zipper ◽  
Blast Disease ◽  
Bzip Transcription Factor ◽  
Wild Type ◽  
Basic Leucine Zipper

Magnaporthe oryzae is the causal agent of rice blast disease, leading to enormous losses of rice production. Here, we characterized a basic leucine zipper (bZIP) transcription factor, Moatf1, in M. oryzae, a homolog of Schizosaccharomyces pombe ATF/CREB that regulates the oxidative stress response. Moatf1 deletion caused retarded vegetative growth of mycelia, and the Moatf1 mutant exhibited higher sensitivity to hydrogen peroxide (H2O2) than did the wild-type strain. The mutant showed severely reduced activity of extracellular enzymes and transcription level of laccases and peroxidases and exhibited significantly reduced virulence on rice cultivar CO-39. On rice leaf sheath, most of the infectious hyphae of the mutant became swollen and displayed restricted growth in primary infected cells. Defense response was strongly activated in plants infected by the mutant. Diamino benzidine staining revealed an accumulation of H2O2 around Moatf1 mutant appressoria and rice cells with Moatf1 hyphae that was absent in the wild type. Inhibition of the plant NADPH oxidase by diphenyleneiodonium prevented host-derived H2O2 accumulation and restored infectious hyphal growth of the mutant in rice cells. Thus, we conclude that Moatf1 is necessary for full virulence of M. oryzae by regulating the transcription of laccases and peroxidases to impair reactive oxygen species–mediated plant defense.

scholarly journals The bZIP transcription factor FpAda1 is essential for fungal growth and conidiation in Fusarium pseudograminearum

2019 ◽  
Vol 66 (3) ◽  
pp. 507-515 ◽  
Author(s):  
Linlin Chen ◽  
Yuming Ma ◽  
Jingya Zhao ◽  
Xuejing Geng ◽  
Wenbo Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leucine Zipper ◽  
Hyphal Growth ◽  
Crown Rot ◽  
Bzip Transcription Factor ◽  
Economic Losses ◽  
Wheat Coleoptile ◽  
Head Blight ◽  
Basic Leucine Zipper ◽  
Fusarium Pseudograminearum

Abstract Fusarium pseudograminearum is an important pathogen of Fusarium crown rot and Fusarium head blight, which is able to infect wheat and barley worldwide, causing great economic losses. Transcription factors (TFs) of the basic leucine zipper (bZIP) protein family control important processes in all eukaryotes. In this study, we identified a gene, designated FpAda1, encoding a bZIP TF in F. pseudograminearum. The homolog of FpAda1 is also known to affect hyphal growth in Neurospora crassa. Deletion of FpAda1 in F. pseudograminearum resulted in defects in hyphal growth, mycelial branching and conidia formation. Pathogenicity assays showed that virulence of the Δfpada1 mutant was dramatically decreased on wheat coleoptiles and barley leaves. However, wheat coleoptile inoculation assay showed that Δfpada1 could penetrate and proliferate in wheat cells. Moreover, the FpAda1 was required for abnormal nuclear morphology in conidia and transcription of FpCdc2 and FpCdc42. Taken together, these results indicate that FpAda1 is an important transcription factor involved in growth and development in F. pseudograminearum.

scholarly journals The Common Bean (Phaseolus vulgaris) Basic Leucine Zipper (bZIP) Transcription Factor Family: Response to Salinity Stress in Fertilized and Symbiotic N2-Fixing Plants

Agriculture ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 160 ◽  
Author(s):  
Litzy Ayra ◽  
Mario Ramírez ◽  
Luis Íñiguez ◽  
Rosa Rodés ◽  
Eduardo Ortega ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Leucine Zipper ◽  
Bzip Transcription Factor ◽  
N Fixation ◽  
Transcription Factor Family ◽  
Basic Leucine Zipper ◽  
The Common ◽  
Bzip Transcription Factor Family

The basic leucine zipper (bZIP) transcription factor family regulates plant developmental processes and response to stresses. The common bean (Phaseolus vulgaris), an important crop legume, possesses a whole set of 78 bZIP (PvbZIP) genes, the majority of these (59%) are most highly expressed in roots and nodules, root-derived new organs formed in the rhizobia N2-fixing symbiosis. Crop production is highly affected by salinity in Cuba and other countries. In this work we characterized the adverse effect of salinity to common bean plants of the Cuban CC-25-9-N cultivar grown in fertilized (full N-content) or symbiotic N-fixation (rhizobia inoculated) conditions. We assessed if PvbZIP TF participate in CC-25-9-N common bean response to salinity. Quantitative reverse-transcriptase-PCR (qRT-PCR) expression analysis showed that 26 out of 46 root/nodule-enhanced PvbZIP, that responded to salt stress in roots and/or nodules from fertilized and N2-fixing CC-25-9-N plants. From public common bean transcriptomic data, we identified 554 genes with an expression pattern similar to that of salt-responsive PvbZIP genes, and propose that the co-expressed genes are likely to be involved in the stress response. Our data provide a foundation for evaluating the individual roles of salt-responsive genes and to explore the PvbZIP-mediated improvement of salt tolerance in common bean.

scholarly journals Basic Leucine zipper (bZIP) transcription factor genes and their response to drought stress in ginseng, Panax ginseng C.A. Meyer

2020 ◽  
Author(s):  
Hongjie Li ◽  
Jing Chen ◽  
Qi Zhao ◽  
Yilai Han ◽  
Chunyu Sun ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Leucine Zipper ◽  
Bzip Transcription Factor ◽  
Functional Categories ◽  
Basic Leucine Zipper ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Transcription Factor Genes ◽  
Alternatively Spliced

Abstract Background: As a famous and important medicinal herb in the world, ginseng contains numerous bioactive components that are remarkable for mankind's health. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant response to abiotic and biotic stresses. Nevertheless, these genes remain unknown in ginseng. Results: Here, we report 91 bZIP genes, designated as PgbZIP genes, identified from ginseng. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into a number of functional categories, suggesting that they have substantially diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed quite differentially across 14 different tissues, the roots of different aged, and the roots of different cultivars. However, the expression of these transcripts was coordinated as they formed a co-expression network. Furthermore, we studied their response to drought stress in ginseng using five representatives of the PgbZIP genes, including PgbZIP25 , PgbZIP38 , PgbZIP39 , PgbZIP53 and PgbZIP54 . The results showed that these PgbZIP genes all responded to drought stress in ginseng, but the magnitudes of their response to drought stress varied. Conclusions: These results provide knowledge and resources for deeper functional analysis of PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.

Light-Induced Artemisinin Biosynthesis Is Regulated by the bZIP Transcription Factor AaHY5 in Artemisia annua

2019 ◽  
Vol 60 (8) ◽  
pp. 1747-1760 ◽  
Author(s):  
Xiaolong Hao ◽  
Yijun Zhong ◽  
Hans-Wilhelm N�tzmann ◽  
Xueqing Fu ◽  
Tingxiang Yan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leucine Zipper ◽  
Artemisia Annua ◽  
Glandular Trichome ◽  
Bzip Transcription Factor ◽  
Light Conditions ◽  
Basic Leucine Zipper ◽  
Ubiquitin E3 Ligase ◽  
Changing Light ◽  
Artemisinin Biosynthesis

Abstract Artemisinin, the frontline drug against malaria, is a sesquiterpenoid extracted from Artemisia annua. Light has been proposed to play an important role in the activation of artemisinin biosynthesis. Here, we report the basic leucine zipper transcription factor (TF) AaHY5 as a key regulator of light-induced biosynthesis of artemisinin. We show that AaHY5 transcription overlaps with that of artemisinin biosynthesis genes in response to light and in A. annua tissues. Analysis of AaHY5 overexpression and RNAi-suppression lines suggests that AaHY5 is a positive regulator of the expression of artemisinin biosynthesis genes and accumulation of artemisinin. We show that AaHY5 complements the hy5 mutant in Arabidopsis thaliana. Our data further suggest that AaHY5 interacts with AaCOP1, the ubiquitin E3 ligase CONSTITUTIVE PHOTOMORPHOGENIC1 in A. annua. In yeast one-hybrid and transient expression assays, we demonstrate that AaHY5 acts via the TF GLANDULAR TRICHOME-SPECIFIC WRKY 1 (AaGSW1) in artemisinin regulation. In summary, we present a novel regulator of artemisinin gene expression and propose a model in which AaHY5 indirectly controls artemisinin production in response to changing light conditions.

Sign in / Sign up

Export Citation Format

Share Document