scholarly journals Basic Leucine zipper (bZIP) transcription factor genes and their response to drought stress in ginseng, Panax ginseng C.A. Meyer

2020 ◽  
Author(s):  
Hongjie Li ◽  
Jing Chen ◽  
Qi Zhao ◽  
Yilai Han ◽  
Chunyu Sun ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Leucine Zipper ◽  
Bzip Transcription Factor ◽  
Functional Categories ◽  
Basic Leucine Zipper ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Transcription Factor Genes ◽  
Alternatively Spliced

Abstract Background: As a famous and important medicinal herb in the world, ginseng contains numerous bioactive components that are remarkable for mankind's health. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant response to abiotic and biotic stresses. Nevertheless, these genes remain unknown in ginseng. Results: Here, we report 91 bZIP genes, designated as PgbZIP genes, identified from ginseng. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into a number of functional categories, suggesting that they have substantially diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed quite differentially across 14 different tissues, the roots of different aged, and the roots of different cultivars. However, the expression of these transcripts was coordinated as they formed a co-expression network. Furthermore, we studied their response to drought stress in ginseng using five representatives of the PgbZIP genes, including PgbZIP25 , PgbZIP38 , PgbZIP39 , PgbZIP53 and PgbZIP54 . The results showed that these PgbZIP genes all responded to drought stress in ginseng, but the magnitudes of their response to drought stress varied. Conclusions: These results provide knowledge and resources for deeper functional analysis of PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.

scholarly journals Basic leucine zipper (bZIP) transcription factor genes and their responses to drought stress in ginseng, Panax ginseng C.A. Meyer

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hongjie Li ◽  
Jing Chen ◽  
Qi Zhao ◽  
Yilai Han ◽  
Li Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Leucine Zipper ◽  
Bzip Transcription Factor ◽  
Plant Responses ◽  
Basic Leucine Zipper ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Transcription Factor Genes ◽  
Alternatively Spliced

Abstract Background Ginseng is an important medicinal herb in Asia and Northern America. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant responses to abiotic and biotic stresses, such as drought stress. Nevertheless, the genes remain unknown in ginseng. Results Here, we report 91 bZIP genes identified from ginseng, designated PgbZIP genes. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into five functional subcategories, suggesting that they have diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed differentially across 14 tissues, the roots of different ages and the roots of different genotypes. However, the transcripts of the genes expressed coordinately and were more likely to form a co-expression network. Furthermore, we studied the responses of the PgbZIP genes to drought stress in ginseng using a random selection of five PgbZIP genes, including PgbZIP25, PgbZIP38, PgbZIP39, PgbZIP53 and PgbZIP54. The results showed that all five PgbZIP genes responded to drought stress in ginseng, indicating that the PgbZIP genes play important roles in ginseng responses to drought stress. Conclusions These results provide knowledge and gene resources for deeper functional analysis of the PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.

scholarly journals Identification and characterization of the bZIP transcription factor family in yellowhorn

2020 ◽  
Vol 32 (1) ◽  
pp. 273-284 ◽  
Author(s):  
Qiaoying Chang ◽  
Xin Lu ◽  
Zhi Liu ◽  
Zhimin Zheng ◽  
Song Yu
Keyword(s):  
Transcription Factor ◽  
Leucine Zipper ◽  
Molecular Mechanisms ◽  
Bzip Transcription Factor ◽  
Biological Functions ◽  
Transcription Factor Family ◽  
Promoter Regions ◽  
Basic Leucine Zipper ◽  
Biotic Stresses ◽  
Bzip Transcription Factor Family

AbstractThe basic leucine zipper (bZIP) transcription factor family is one of the largest and most diverse families in plants, regulating plant growth and development and playing an essential role in response to abiotic and biotic stresses. However, little is known about the biological functions of bZIP proteins in yellowhorn (Xanthoceras sorbifolium). Recently, 64 XsbZIP genes were identified in the yellowhorn genome and found to be disproportionately distributed in linkage groups. The XsbZIP proteins clustered into 11 groups based on their phylogenetic relationships with AtbZIP, ZmbZIP and GmbZIP proteins. Five intron patterns in the basic and hinge regions and additional conserved motifs were defined, both supporting the group classification and possibly contributing to their functional diversity. Compared to tandem duplication, the segment duplication greatly contributed to the expansion of yellowhorn bZIP genes. In addition, most XsbZIP genes harbor several stress responsive cis-elements in their promoter regions. Moreover, the RNA-seq and qRT-PCR data indicated XsbZIP genes were extensively involved in response to various stresses, including salt (NaCl), cold and abscisic acid, with possibly different molecular mechanisms. These results provide a new understanding of the biological functions of bZIP transcription factors in yellowhorn.

scholarly journals Regulation of Nitrate (NO3) Transporters and Glutamate Synthase-Encoding Genes under Drought Stress in Arabidopsis: The Regulatory Role of AtbZIP62 Transcription Factor

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2149
Author(s):  
Nkulu Kabange Rolly ◽  
Byung-Wook Yun
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Leucine Zipper ◽  
Target Genes ◽  
De Novo ◽  
In Silico Analysis ◽  
Glutamate Synthase ◽  
Regulatory Elements ◽  
Transcript Accumulation ◽  
Basic Leucine Zipper

Nitrogen (N) is an essential macronutrient, which contributes substantially to the growth and development of plants. In the soil, nitrate (NO3) is the predominant form of N available to the plant and its acquisition by the plant involves several NO3 transporters; however, the mechanism underlying their involvement in the adaptive response under abiotic stress is poorly understood. Initially, we performed an in silico analysis to identify potential binding sites for the basic leucine zipper 62 transcription factor (AtbZIP62 TF) in the promoter of the target genes, and constructed their protein–protein interaction networks. Rather than AtbZIP62, results revealed the presence of cis-regulatory elements specific to two other bZIP TFs, AtbZIP18 and 69. A recent report showed that AtbZIP62 TF negatively regulated AtbZIP18 and AtbZIP69. Therefore, we investigated the transcriptional regulation of AtNPF6.2/NRT1.4 (low-affinity NO3 transporter), AtNPF6.3/NRT1.1 (dual-affinity NO3 transporter), AtNRT2.1 and AtNRT2.2 (high-affinity NO3 transporters), and AtGLU1 and AtGLU2 (both encoding glutamate synthase) in response to drought stress in Col-0. From the perspective of exploring the transcriptional interplay of the target genes with AtbZIP62 TF, we measured their expression by qPCR in the atbzip62 (lacking the AtbZIP62 gene) under the same conditions. Our recent study revealed that AtbZIP62 TF positively regulates the expression of AtPYD1 (Pyrimidine 1, a key gene of the de novo pyrimidine biosynthesis pathway know to share a common substrate with the N metabolic pathway). For this reason, we included the atpyd1-2 mutant in the study. Our findings revealed that the expression of AtNPF6.2/NRT1.4, AtNPF6.3/NRT1.1 and AtNRT2.2 was similarly regulated in atzbip62 and atpyd1-2 but differentially regulated between the mutant lines and Col-0. Meanwhile, the expression pattern of AtNRT2.1 in atbzip62 was similar to that observed in Col-0 but was suppressed in atpyd1-2. The breakthrough is that AtNRT2.2 had the highest expression level in Col-0, while being suppressed in atbzip62 and atpyd1-2. Furthermore, the transcript accumulation of AtGLU1 and AtGLU2 showed differential regulation patterns between Col-0 and atbzip62, and atpyd1-2. Therefore, results suggest that of all tested NO3 transporters, AtNRT2.2 is thought to play a preponderant role in contributing to NO3 transport events under the regulatory influence of AtbZIP62 TF in response to drought stress.

scholarly journals The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Jian-Ping An ◽  
Feng-Jia Qu ◽  
Ji-Fang Yao ◽  
Xiao-Na Wang ◽  
Chun-Xiang You ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nitrate Reductase ◽  
Leucine Zipper ◽  
Anthocyanin Biosynthesis ◽  
Nitrate Assimilation ◽  
Bzip Transcription Factor ◽  
Anthocyanin Accumulation ◽  
Mobility Shift ◽  
Basic Leucine Zipper ◽  
Transgenic Apple

Abstract The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs, respectively. Furthermore, we obtained transgenic apple calli that overexpressed the MdHY5 gene, and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis genes. In addition, the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were detected. In association with increased nitrate reductase activities and nitrate contents, the results indicated that MdHY5 might be an important regulator in nutrient assimilation. Taken together, these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.

scholarly journals Metallothionein and bZIP Transcription Factor Genes from Velvetleaf and Their Differential Expression Following Colletotrichum coccodes Infection

2006 ◽  
Vol 96 (10) ◽  
pp. 1116-1123 ◽  
Author(s):  
Amélie L. Dauch ◽  
Suha H. Jabaji-Hare
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Leucine Zipper ◽  
Expression Patterns ◽  
Bzip Transcription Factor ◽  
Colletotrichum Coccodes ◽  
Transcription Factor Genes ◽  
Pathogen Attack ◽  
Fungal Genes ◽  
Type 3

Colletotrichum coccodes is a biocontrol agent of velvetleaf (Abutilon theophrasti), a noxious weed of corn and soybean. Metallothioneins (MTs) and basic region/leucine zipper motif (bZIP) are heavy-metal-binding proteins and transcription factors, respectively, that have been related to several plant processes, including the responses of plants to pathogen attack. Previous investigation of the determinants involved in the velvet-leaf-C. coccodes interaction had shed light on particular plant and fungal genes expressed in this pathosystem. Here, we report on the temporal expression patterns of two distinct types (2 and 3) of MT and bZIP transcription factor genes in velvetleaf leaves following infection with C. coccodes using quantitative reverse-transcription polymerase chain reaction. Gene expression ratios were significantly upregulated 1 day after infection (DAI), a time at which velvetleaf leaves appeared symptomless. At 2 DAI, bZIP and type 3 MT expression ratios dropped to levels significantly lower than those estimated for noninfected plants. Necrotic symptoms appeared 5 DAI and increased with time, during which gene expression levels were maintained either below or at levels observed in the control. These findings indicate that C. coccodes altered the expression of type 2 and 3 MT and bZIP genes. In addition, this is the first report on induction of a type 3 MT in plants in response to a pathogen attack.

scholarly journals Genome-wide identification and analysis of the basic leucine zipper (bZIP) transcription factor gene family in Ustilaginoidea virens

Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1051-1059 ◽  
Author(s):  
Weixiao Yin ◽  
Peng Cui ◽  
Wei Wei ◽  
Yang Lin ◽  
Chaoxi Luo
Keyword(s):  
Transcription Factor ◽  
Phylogenetic Relationships ◽  
Leucine Zipper ◽  
Expression Profiles ◽  
Pathogenic Fungi ◽  
Bzip Transcription Factor ◽  
Basic Leucine Zipper ◽  
Ustilaginoidea Virens ◽  
Infection Period ◽  
Genome Wide

The basic leucine zipper (bZIP) transcription factor (TF) family is one of the largest and most diverse TF families widely distributed across the eukaryotes. The bZIP TF family plays an important role in growth, development, and response to abiotic or biotic stresses, which have been well characterized in plants, but not in plant pathogenic fungi. In this study, we performed genome-wide and systematic bioinformatics analysis of bZIP genes in the fungus Ustilaginoidea virens, the causal agent of rice false smut disease. We identified 28 bZIP family members in the U. virens genome by searching for the bZIP domain in predicted genes. The gene structures, motifs, and phylogenetic relationships were analyzed for bZIP genes in U. virens (UvbZIP). Together with bZIP proteins from two other fungi, the bZIP genes can be divided into eight groups according to their phylogenetic relationships. Based on RNA-Seq data, the expression profiles of UvbZIP genes at different infection stages were evaluated. Results showed that 17 UvbZIP genes were up-regulated during the infection period. Furthermore, 11 infection-related UvbZIP genes were investigated under H2O2 stress and the expression level of eight genes were changed, which confirmed their role in stress tolerance and pathogenicity. In summary, our genome-wide systematic characterization and expression analysis of UvbZIP genes provided insight into the molecular function of these genes in U. virens and provides a reference for other pathogens.

scholarly journals Sucrose-induced translational repression of plant bZIP-type transcription factors

2005 ◽  
Vol 33 (1) ◽  
pp. 272-275 ◽  
Author(s):  
A. Wiese ◽  
N. Elzinga ◽  
B. Wobbes ◽  
S. Smeekens
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Translational Control ◽  
Leucine Zipper ◽  
Upstream Open Reading Frame ◽  
Bzip Transcription Factor ◽  
Reading Frame ◽  
Signalling Molecules ◽  
Transcription Factor Genes ◽  
Plant Genes

Sugars as signalling molecules exert control on the transcription of many plant genes. Sugar signals also alter mRNA and protein stability. Increased sucrose concentrations specifically repress translation of the S-class basic region leucine zipper (bZIP) type transcription factor AtbZIP11/ATB2. This sucrose-induced repression of translation (SIRT) depends on translation of a highly conserved upstream open reading frame (uORF) in the 5′ UTR of the gene. This conserved uORF is exclusively encoded in 5′ UTRs of several plant S-class bZIP transcription factors. Arabidopsis homologues of ATB2/AtbZIP11, which harbour the conserved uORF, also show SIRT. Therefore, SIRT emerges as a general sucrose translational control mechanism of a group of transcription factors. SIRT might be part of a sucrose-specific signalling pathway, controlling expression of plant bZIP transcription factor genes.

scholarly journals The Basic Leucine Zipper Transcription Factor Moatf1 Mediates Oxidative Stress Responses and Is Necessary for Full Virulence of the Rice Blast Fungus Magnaporthe oryzae

2010 ◽  
Vol 23 (8) ◽  
pp. 1053-1068 ◽  
Author(s):  
Min Guo ◽  
Wang Guo ◽  
Yue Chen ◽  
Suomeng Dong ◽  
Xing Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Magnaporthe Oryzae ◽  
Stress Responses ◽  
Rice Blast ◽  
Leucine Zipper ◽  
Blast Disease ◽  
Bzip Transcription Factor ◽  
Wild Type ◽  
Basic Leucine Zipper

Magnaporthe oryzae is the causal agent of rice blast disease, leading to enormous losses of rice production. Here, we characterized a basic leucine zipper (bZIP) transcription factor, Moatf1, in M. oryzae, a homolog of Schizosaccharomyces pombe ATF/CREB that regulates the oxidative stress response. Moatf1 deletion caused retarded vegetative growth of mycelia, and the Moatf1 mutant exhibited higher sensitivity to hydrogen peroxide (H2O2) than did the wild-type strain. The mutant showed severely reduced activity of extracellular enzymes and transcription level of laccases and peroxidases and exhibited significantly reduced virulence on rice cultivar CO-39. On rice leaf sheath, most of the infectious hyphae of the mutant became swollen and displayed restricted growth in primary infected cells. Defense response was strongly activated in plants infected by the mutant. Diamino benzidine staining revealed an accumulation of H2O2 around Moatf1 mutant appressoria and rice cells with Moatf1 hyphae that was absent in the wild type. Inhibition of the plant NADPH oxidase by diphenyleneiodonium prevented host-derived H2O2 accumulation and restored infectious hyphal growth of the mutant in rice cells. Thus, we conclude that Moatf1 is necessary for full virulence of M. oryzae by regulating the transcription of laccases and peroxidases to impair reactive oxygen species–mediated plant defense.

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