OBSERVATIONS ON THE CULTURE AND MAINTENANCE OF A FREE-LIVING AMOEBA
Keyword(s):
The Past
◽
A free-living amoeba isolated from creek water could be grown at 24 °C. on a solid malt agar medium together with a pure culture of a motile Gram-negative bacillus, isolated from the same sample of water. The bacilli apparently served as the sole source of food for the trophozoites, the malt in the medium being-utilized by the bacilli for their own growth. In addition, the encysted form has been maintained on this medium at 24 °C. for the past 12 months and at 4 °C. for 10 months. Transfers to fresh medium of cysts from such cultures yielded numerous trophozoites after 12 hr. incubation at 24 °C, provided that metabolically active bacilli were present.
2019 ◽
Vol 19
(12)
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pp. 980-987
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2020 ◽
Keyword(s):
2020 ◽
Vol 48
(8)
◽
pp. 030006052092986