EXTENDED STUDY OF SERRATIA IN A DIAGNOSTIC BACTERIOLOGY LABORATORY

1966 ◽  
Vol 12 (1) ◽  
pp. 99-103 ◽  
Author(s):  
R. M. Hotz ◽  
V. R. Dowell Jr.
Keyword(s):  
Polymyxin B ◽  
Biochemical Tests ◽  
Diagnostic Laboratory ◽  
Extended Study ◽  
Clinical Specimens ◽  
Hospital Acquired Infections ◽  
Noticeable Increase ◽  
Routine Identification ◽  
Hospital Acquired ◽  
Antibiotic Agents

The incidence of Serratia in clinical material received in a general hospital diagnostic laboratory was studied. Initially the morphologic features, biochemical characteristics, chromogenesis, and antibiotic susceptibility of 21 isolates were examined. On the basis of these studies certain cultural and biochemical tests were selected for routine identification of Serratia.Serratia isolated from various types of clinical specimens received during a 4-year period (1960–1964) were recorded. Most of the Serratia were nonchromogenic except on a special medium. The cultures were resistant to a number of antibiotic agents, particularly penicillin, polymyxin B, and the tetracyclines. A higher incidence of Serratia in sputum than in throat and nasopharyngeal cultures was observed. There was a noticeable increase in the overall incidence of Serratia isolates over the 4-year period. This may be a reflection of hospital acquired infections.

scholarly journals Use of 16s rRNA to identify non-lactose-fermenting bacilli and molecular detection of ESBL resistance genes associated with hospital-acquired infection in Soba University Hospital, Sudan

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1311
Author(s):  
Wissam Ahmed Al Hag ◽  
Hana Elbadawi ◽  
Muzamil Mahdi Abdel Hamid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Resistance Genes ◽  
University Hospital ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Hospital Acquired Infections ◽  
Hospital Acquired

Background: Non-lactose-fermenting gram-negative bacilli (NLFGNB) have become significant nosocomial pathogens and often exhibit intrinsic multidrug resistance. Sequencing of 16s rRNA genes could be utilized for robust identification of NLFGNB. This study aimed to identify resistant NLFGNB associated with hospital-acquired infections using 16s rRNA sequencing and to detect the extended-spectrum β-lactamase (ESBL) genes of isolates in Soba Hospital, Khartoum State, Sudan. Methods: A prospective, cross-sectional, laboratory-based study was conducted from October 2017 to March 2018 at the Microbiology Department of Soba University Hospital. A total of 100 randomly selected NLFGNB samples were isolated from blood and urine during the time of the study. All the isolates were identified using standard biochemical tests and antimicrobial sensitivity testing, 16s rRNA gene sequencing, and bioinformatics techniques. Results: The biochemical tests revealed that, out of the 100 NLFGNB isolates, the Pseudomonas species was predominant (57 isolates), followed by gram-negative bacilli (33 isolates), Coccobacilli (9 isolates) and Coliform (1 isolate) species. Sequencing of 16s rRNA genes identified all the resistant isolates at the species level: Pseudomonas aeruginosa (26%), Acinetobacter baumannii (22%), Burkholderia cepacia (13%), Stenotrophomonas maltophilia (10%), Enterococcus species (E. faecalis, E. faecium) (10%), and other GNB (Acinetobacter variabilis, Klebsiella pneumoniae, Morganella morganii, Escherichia fergusonii, Enterobacter hormaechei and Pseudomonas stutzeri) (19%). The antimicrobial susceptibility tests indicated that 31 isolates were resistant to at least three classes of antibiotics and contain the highest level of ESBL resistance genes. Conclusions: Pseudomonas aeruginosa and Acinetobacter baumannii were the most widely recognized NLFGNB identified from hospital-acquired infections in Soba hospital. Among the NLFGNB, antimicrobial resistance and ESBL resistance genes were observed at a high frequency.

scholarly journals A study of Acinetobacter infections in a tertiary care hospital in Northeast India

2018 ◽  
Vol 6 (6) ◽  
pp. 2076
Author(s):  
Swarnatrisha Saha ◽  
Ksh Mamta Devi ◽  
Shan Damrolien ◽  
Kh Sulochana Devi
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care ◽  
Empirical Therapy ◽  
Resistance Pattern ◽  
Care Hospital ◽  
Acinetobacter Species ◽  
Clinical Specimens ◽  
Hospital Acquired Infections ◽  
Antibiotic Susceptibility Test ◽  
Hospital Acquired

Background: Acinetobacter is an important opportunistic pathogen and is a common cause of hospital acquired infections. Acinetobacter infections are often extremely difficult to treat because of their widespread resistance to the major groups of antibiotics. The study was conducted to determine prevalence and antibiotic susceptibility pattern of Acinetobacter species isolated from various clinical samples.Methods: Clinical specimens over a period of 2yrs from May 2015 to April 2017 were collected from the patients attending the hospital. Acinetobacter species isolates were identified, and antibiotic susceptibility test was done following standard operative procedures.Results: From 9979 clinical specimens, 3715 were positive for significant bacterial growth of which 111 (2.9%) were culture positive for Acinetobacter spp. Among 111 isolates 109 (98.2%) isolates were Acinetobacter baumanni and 2 (1.8%) were Acinetobacter lwoffii. Maximum isolates were isolated from urine samples 36 (32.4%) and majority of the isolates were from wards (56.7%) giving a probability of increased hospital acquired infections. Maximum resistance was shown by cefipime (80.1%). Imipenem and Meropenem shows resistance of 25.3% and 29.7% respectively. ICU isolates showed extensive resistance in comparison to wards and OPD.Conclusions: Increasing trend of resistance pattern to a large range of antibiotics is a matter of concern. To avoid resistance, antibiotics should be used judiciously, and empirical therapy should be determined for each hospital according to the resistance rates of the hospital. Infection with MDR Acinetobacter species is independently associated with high mortality, emphasizing the need for aggressive infection control strategies.

scholarly journals INVESTIGATION OF THE ANTIBIOTIC RESISTANCE: THE CASE OF BUU DIEN GENERAL HOSPITAL IN HO CHI MINH CITY

2019 ◽  
pp. 116-119
Author(s):  
Tran-Thi Ngoc-Van ◽  
Tran Quang-Thinh ◽  
Cu Thanh-Tuyen ◽  
Hoang-Thy Nhac-Vu
Keyword(s):  
Antibiotic Resistance ◽  
General Hospital ◽  
Susceptibility Testing ◽  
Antibiotic Use ◽  
Polymyxin B ◽  
Ho Chi Minh City ◽  
Cross Sectional ◽  
E Coli ◽  
Hospital Acquired Infections ◽  
Hospital Acquired

Objective: In Vietnam, antibiotic resistance has been gained the attention of medical professionals in antibiotic use management. This study aimed to investigate the antibiotic resistance among hospital-acquired infections at Buu Dien General Hospital in Ho Chi Minh City in the period of 01-12/2017. Methods: This cross-sectional descriptive study was conducted on the retrospective data of all antibiograms of bacteria isolated from hospital-acquired infections at Buu Dien General Hospital in Ho Chi Minh City in the period of 01-12/2017 to investigate the antibiotic resistance. Characteristics of antibiotic resistance were described by frequency and percentage of types of bacteria isolated and antibiotics being resistant. Results: A total of 179 isolates were collected during the period 01-12/2017, of which E. coli was the most commonly isolated pathogen (41.3%). The highest prevalent infections were in the skin and mucosa; respiratory tract; and urinary tract (34.6%; 32.4%; and 27.9%). The antibiotic susceptibility testing used 21 types of antibiotics. Among them, S. aureus was 82% resistant to clindamycin and 75% resistant to cefuroxime; the Proteus resistance percentages to amoxicillin/clavulanic, second-generation cephalosporins, ciprofloxacin and fosfomycin varied from 50 to 93%; Pseudomonas was 92% resistant to fosfomycin and 62% resistant to ceftazidime; A. baumannii was resistant to most classes of agents used (50-75%). Both E. coli and Klebsiella were highly resistant to gentamicin, amoxicillin, ciprofloxacin, 2nd and 3rd generation cephalosporin’s. Polymyxin B-resistant Proteus cultures were detected at 67%. Conclusion: The study described the antibiotic resistance situation of hospital-acquired bacteria at the Buu Dien General Hospital from 01-12/2017. This information will aid physicians to select proper antibiotics for their patients in the next period.

scholarly journals The increasing isolation ofSerratiaspecies from clinical specimens

1979 ◽  
Vol 82 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Heather J. L. Brooks ◽  
T. J. Chambers ◽  
Soad Tabaqchali
Keyword(s):  
Antibiotic Resistance ◽  
Cross Infection ◽  
Multiple Antibiotic Resistance ◽  
Gram Negative ◽  
Clinical Specimens ◽  
Hospital Acquired Infections ◽  
Gram Negative Organisms ◽  
Hospital Acquired ◽  
Simple Screening

SUMMARYA 14-month survey was undertaken in a diagnostic bacteriology laboratory to determine the incidence ofSerratiaspp. in routine clinical specimens. Gram-negative organisms with enterobacteria-like colonies were tested by a simple screening procedure. Fifty-eight strains ofS. marcescensand two strains ofS. liquefacienswere isolated from 59 patients. The strains were usually nonpigmented and exhibited multiple antibiotic resistance. Serotyping and determination of bacteriocine sensitivity patterns revealed that the majority of infections were sporadic, although episodes of cross-infection did occur.S. marcescenswas considered to contribute significantly to morbidity and mortality in 53% of patients and appears to be of increasing importance in hospital-acquired infections.

Isolation of post operative hospital acquired infections in a private hospital of Bangladesh and identification of clonal relatedness of E. coli by using Pulse filed gel electrophoresis

2012 ◽  
Vol 6 (2) ◽  
pp. 7-10
Author(s):  
Mohammad Murshed ◽  
Sabeena Shahnaz ◽  
Md. Abdul Malek
Keyword(s):  
Private Hospital ◽  
Isolation And Identification ◽  
College Hospital ◽  
Hospital Acquired Infection ◽  
E Coli ◽  
Hospital Acquired Infections ◽  
Medical College ◽  
Clonal Relatedness ◽  
Hospital Acquired ◽  
Dressing Materials

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10

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