Mutants of Bacillus cereus strain T that produce thermoresistant spores lacking dipicolinate and have low levels of calcium

1972 ◽  
Vol 18 (7) ◽  
pp. 1139-1143 ◽  
Author(s):  
R. S. Hanson ◽  
M. V. Curry ◽  
J. V. Garner ◽  
H. Orin Halvorson
Keyword(s):  
Bacillus Cereus ◽  
Liquid Medium ◽  
Dipicolinic Acid ◽  
Distilled Water ◽  
Bacterial Endospores ◽  
Low Levels

Mutants of Bacillus cereus strain T that produce thermoresistant spores which lack dipicolinic acid and have low levels of calcium and manganese have been isolated. The thermoresistant dipicolinate-less spores lose thermoresistance upon storage in distilled water and do not germinate in any liquid medium tested. All spores germinated and produced colonies on G medium agar. It is suggested that calcium and dipicolinic acid are not required for the acquisition of thermoresistance in bacterial endospores but that they are essential to the maintenance of the thermorésistant state and are required for normal germination.

SOME CHARACTERISTICS OF SPORES OF BACILLUS CEREUS PRODUCED BY A REPLACEMENT TECHNIQUE

1963 ◽  
Vol 9 (2) ◽  
pp. 251-258 ◽  
Author(s):  
Elisabeth Ann Pelcher ◽  
H. P. Fleming ◽  
Z. John Ordal
Keyword(s):  
Heat Resistance ◽  
Bacillus Cereus ◽  
Dipicolinic Acid ◽  
Fresh Medium ◽  
Distilled Water ◽  
Normal Complement ◽  
Strontium Barium ◽  
Replacement Technique ◽  
Low Levels ◽  
The Relationship

A replacement technique, whereby spores of Bacillus cereus were produced by transferring vegetative cells into non-nutritive solutions, was employed for the investigation of the relationship between calcium, dipicolinic acid (DPA), and heat resistance in spores. Replacement solutions of distilled water, fresh medium, calcium, strontium, barium, nickel, and other metals were employed. Spores produced in distilled water were heat sensitive and contained low levels of calcium and DPA. Heat-resistant spores with a normal complement of calcium and DPA were formed only in fresh medium or in solutions containing suitable levels of calcium. Calcium and DPA were present in the spores in nearly equimolar quantities. Of the other metals tested, only strontium stimulated DPA synthesis above the level of the spores produced in water. Spores of intermediary heat resistance but low DPA content were formed in solutions of barium and nickel.

CALCIUM REVERSAL OF THE HEAT SUSCEPTIBILITY AND DIPICOLINATE DEFICIENCY OF SPORES FORMED "ENDOTROPHICALLY" IN WATER

1960 ◽  
Vol 6 (2) ◽  
pp. 213-224 ◽  
Author(s):  
Samuel H. Black ◽  
Tadayo Hashimoto ◽  
Philipp Gerhardt
Keyword(s):  
Gamma Radiation ◽  
Bacillus Cereus ◽  
Calcium Ions ◽  
Dipicolinic Acid ◽  
Distilled Water ◽  
Vegetative Cells ◽  
Low Concentration

Spores of Bacillus cereus strain terminalis formed "endotrophically" by transferring granular vegetative cells to distilled water were found to be relatively susceptible to heat and deficient in dipicolinic acid. Calcium ions alone, added in low concentration shortly after the cells were placed in water, could completely relieve these abnormalities. Although the water-formed spores were sensitive to heat, they were as fully resistant as normal spores to gamma radiation or phenol.

The Effect of Copper Ions on Clot Lysis Time in the Fibrinolytic Assay of Streptokinase

1965 ◽  
Vol 13 (02) ◽  
pp. 477-483
Author(s):  
Alwin B. Bogert
Keyword(s):  
Copper Ions ◽  
Borate Buffer ◽  
Fibrinolytic System ◽  
Clot Lysis ◽  
Distilled Water ◽  
Naturally Occurring ◽  
Lysis Time ◽  
Low Levels ◽  
Effect Of Copper ◽  
Clot Lysis Time

SummaryExperiments were conducted to determine why different lots of Borate Buffer reagent affect the clot lysis times obtained in the fibrinolytic assay of Streptokinase. Minerals naturally occurring in distilled water were screened individually to determine their influence on lysis. Copper was found to have a very pronounced effect in this regard on the fibrinolytic system in that low levels reduce the lysis time and high levels increase it.

Effects of Cold Atmospheric Pressure Plasma Jeton the Viability of Bacillus subtilis Endospores

2013 ◽  
Vol 647 ◽  
pp. 524-531
Author(s):  
Vinita Sharma ◽  
Katsuhiko Hosoi ◽  
Tamio Mori ◽  
Shin-ichi Kuroda
Keyword(s):  
Bacillus Subtilis ◽  
Atmospheric Pressure ◽  
Dipicolinic Acid ◽  
Atmospheric Pressure Plasma ◽  
Dilution Method ◽  
Pressure Plasma ◽  
Agar Disc ◽  
Bacterial Endospores ◽  
Cold Atmospheric Pressure Plasma ◽  
Ar Gas

In this study, we conducted experiments to investigate the effectiveness of a non-equilibrium Ar-N2 plasma jet generated by a Cold Atmospheric Pressure Plasma Torch (CAPPLAT) at a sinusoidal voltage of 20 kV, frequency of 30 kHz with 10 slm of Ar gas and 100 sccm of N2 gas. Highly environmental stress resistant bacterial endospores of Bacillus subtilis, dried on an agar disc were exposed to the plasma discharge from the CAPPLAT for different durations. The viability of spores after plasma exposure was checked by counting CFUs by serial dilution method. We also measured the amount of released DPA (dipicolinic acid, pyridine-2, 6-dicarboxylic acid), which is exclusively found in endospore protoplast (cortex), to confirm the disintegration of the cortex. We could successfully inactivate a population of Bacillus endospores of about 1.0 × 107 to 4.0 × 107 spores/ml.

Gold nanoparticles having dipicolinic acid imprinted nanoshell for Bacillus cereus spores recognition

2009 ◽  
Vol 256 (1) ◽  
pp. 142-148 ◽  
Author(s):  
Aytaç Gültekin ◽  
Arzu Ersöz ◽  
Deniz Hür ◽  
Nalan Yılmaz Sarıözlü ◽  
Adil Denizli ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bacillus Cereus ◽  
Dipicolinic Acid

Postharvest handling of cut immature Eucalyptus foliage

1993 ◽  
Vol 33 (5) ◽  
pp. 663 ◽  
Author(s):  
RB Jones ◽  
JK Truett ◽  
M Hill
Keyword(s):  
Active Chlorine ◽  
Vase Life ◽  
Distilled Water ◽  
Dry Storage ◽  
Wet Storage ◽  
Postharvest Handling ◽  
Low Levels ◽  
Exogenous Ethylene ◽  
Dry Transport ◽  
And Storage

Optimum postharvest and storage treatments were investigated for cut immature branches of Eucalyptus crenulata Blakely & Beuzev. and Eucalyptus gunnii J. D. Hook. The application of the germicide BCDMH at 10 mg/L of active chlorine, 100 �L Agral-600/L. or 0.25% sucrose to vase solutions significantly enhanced vase life in E. crenulata Longevity in E. gunnii was significantly extended by sucrose (0.25-2% w/v), but not by germicides or Agral. Sucrose pulses (1-10% for 24 11 at 20�C) or exogenous ethylene (50 �L/L for 24 h at 20�C) had no effect on the longevity of either species. Both species produced very low levels of ethylene immediately after harvest and after a 24-h simulation of dry transport at 20�C. Longevity was not significantly altered in either species by 35 days of dry storage at 1�C. or by 7 days of wet storage (branches held in distilled water + 50 mg DICAJL) at 1�C, but declined significantly in both species after 7 days of wet storage at 10�C.

scholarly journals Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment

2020 ◽  
Vol 8 (4) ◽  
pp. 607 ◽  
Author(s):  
Phillip S. Coburn ◽  
Frederick C. Miller ◽  
Morgan A. Enty ◽  
Craig Land ◽  
Austin L. LaGrow ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Virulence Genes ◽  
Ex Vivo ◽  
Brain Heart Infusion ◽  
Luria Bertani ◽  
Ocular Infection ◽  
Rna Seq ◽  
Specific Factors ◽  
Low Levels

Bacillus cereus produces many factors linked to pathogenesis and is recognized for causing gastrointestinal toxemia and infections. B. cereus also causes a fulminant and often blinding intraocular infection called endophthalmitis. We reported that the PlcR/PapR system regulates intraocular virulence, but the specific factors that contribute to B. cereus virulence in the eye remain elusive. Here, we compared gene expression in ex vivo vitreous humor with expression in Luria Bertani (LB) and Brain Heart Infusion (BHI) broth by RNA-Seq. The expression of several cytolytic toxins in vitreous was less than or similar to levels observed in BHI or LB. Regulators of virulence genes, including PlcR/PapR, were expressed in vitreous. PlcR/PapR was expressed at low levels, though we reported that PlcR-deficient B. cereus was attenuated in the eye. Chemotaxis and motility genes were expressed at similar levels in LB and BHI, but at low to undetectable levels in vitreous, although motility is an important phenotype for B. cereus in the eye. Superoxide dismutase, a potential inhibitor of neutrophil activity in the eye during infection, was the most highly expressed gene in vitreous. Genes previously reported to be important to intraocular virulence were expressed at low levels in vitreous under these conditions, possibly because in vivo cues are required for higher level expression. Genes expressed in vitreous may contribute to the unique virulence of B. cereus endophthalmitis, and future analysis of the B. cereus virulome in the eye will identify those expressed in vivo, which could potentially be targeted to arrest virulence.

Measurement of Residual Hydrogen Peroxide in Preformed Food Cartons Decontaminated with Hydrogen Peroxide and Ultraviolet Irradiation

1983 ◽  
Vol 46 (12) ◽  
pp. 1074-1077 ◽  
Author(s):  
CATHERINE J. STANNARD ◽  
JOHN M. WOOD
Keyword(s):  
Hydrogen Peroxide ◽  
Ultraviolet Irradiation ◽  
Food Packaging ◽  
Distilled Water ◽  
Post Process ◽  
Hot Air ◽  
Residual Hydrogen ◽  
Low Levels ◽  
Uv C

A luminometric method was used to determine the levels of residual hydrogen peroxide present in preformed food packaging cartons after a decontamination process using sterile distilled water or 0.1, 1.0 or 30% (wt/vol) hydrogen peroxide and ultraviolet (UV-C, 254 nm) irradiation. The reduction of post-process peroxide levels in the cartons by irradiation or hot air was assessed. A residual hydrogen peroxide level of approx. 100 ppb could be obtained by spraying 0.2 ml of 0.1% hydrogen peroxide into the carton. Treatment with 1% hydrogen peroxide, with or without UV-C irradiation, gave residual levels approximately tenfold higher. The level was not reduced by UV-C irradiation but could be reduced by blowing hot air into the carton. 30% hydrogen peroxide sprayed into cartons could not be reduced by heat to levels below 100 ppb. Extremely low levels of residual hydrogen peroxide were detected when water was sprayed into cartons, both with or without UV-C irradiation.

STUDIES ON THE BIOSYNTHESIS OF DIPICOLINIC ACID IN SPORES OF BACILLUS CEREUS VAR. MYCOIDES1

1955 ◽  
Vol 69 (3) ◽  
pp. 337-346 ◽  
Author(s):  
J. J. Perry ◽  
J. W. Foster
Keyword(s):  
Bacillus Cereus ◽  
Dipicolinic Acid

A Monostyryl Boradiazaindacene (BODIPY)-based lanthanide-free colorimetric and fluorogenic probe for sequential sensing of copper (II) ions and dipicolinic acid as a biomarker of bacterial endospores

2019 ◽  
Vol 377 ◽  
pp. 299-304 ◽  
Author(s):  
Yagmur Cetinkaya ◽  
Mediha Nur Zafer Yurt ◽  
Huseyin Avni Oktem ◽  
M. Deniz Yilmaz
Keyword(s):  
Dipicolinic Acid ◽  
Bacterial Endospores ◽  
Fluorogenic Probe
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