Bioproduction of rubratoxin in a glucose–mineral salts broth with nutritional supplements and metabolic inhibitors

1977 ◽  
Vol 23 (12) ◽  
pp. 1695-1699
Author(s):  
C. Obi Emeh ◽  
Elmer H. Marth
Keyword(s):  
Sodium Azide ◽  
Nitrogen Sources ◽  
Nutritional Supplements ◽  
Metabolic Inhibitors ◽  
Sodium Metabisulfite ◽  
Mold Growth ◽  
Mineral Salts ◽  
Moderate Reduction ◽  
Penicillium Rubrum ◽  
Toxin Formation

A sterile glucose–salts broth fortified with various metabolic inhibitors and nutritional supplements was inoculated with conidia of Penicillium rubrum P3290, and incubated quiescently at 28 °C for 14 days. Potassium sulfite and sodium metabisulfite at all test concentrations caused moderate reduction in rubratoxin formation; at high concentrations (≥2.7 × 10−2 M) accumulation of fungal tissue was also retarded. Production of rubratoxin and cell mass was inhibited by p-aminobenzoic acid; syntheses of toxin were completely blocked by 7.5 × 10−2 M of the vitamin. Effects of sodium fluoride on P. rubrum cultures grown on inorganic nitrogen sources varied from inhibition of mold growth and (or) rubratoxin A production to reduction in formation of rubratoxin B. With organic nitrogen sources, fluoride caused a 30 and 60% reduction in synthesis of rubratoxins A and B, respectively. Sodium acetate at all test concentrations enhanced formation of rubratoxin; mold growth was enhanced when acetate concentration was ≥6.0 × 10−2 M. A moderate reduction in mold growth was caused by lower acetate concentrations (1.2 × 10−2 M or 2.4 × 10−2 M). Sodium arsenite and iodoacetate at test concentrations blocked mold growth and toxin formation; sodium azide and 2,4-dinitrophenol caused a marked reduction in mold growth but inhibited toxin formation completely. However, sodium azide permitted slight growth and toxin formation when mold cultures were incubated for 28 days.

Root Absorption and Translocation of Picloram by Oats and Soybeans

Weed Science ◽  
1971 ◽  
Vol 19 (6) ◽  
pp. 727-731 ◽  
Author(s):  
A. R. Isensee ◽  
G. E. Jones ◽  
B. C. Turner
Keyword(s):  
Glycine Max ◽  
Plant Species ◽  
Sodium Azide ◽  
Avena Sativa ◽  
Sodium Arsenite ◽  
Solution Concentration ◽  
Metabolic Inhibitors ◽  
Root Absorption ◽  
Soybean Roots ◽  
Initial Uptake

The effects of time, concentration, pH, temperature, and metabolic inhibitors on 4-amino-3,5,6-trichloropicolinic acid (picloram) uptake from nutrient solution by oats (Avena sativaL. ‘Markton’) and soybeans (Glycine maxL. ‘Lee’) were studied. Oats and soybeans had similar absorption patterns of rapid initial uptake. However, total accumulation patterns markedly differed in that accumulation was concentration-dependent for oats but not for soybeans. Initial uptake by oats and soybean roots increased as solution concentration increased. Picloram was redistributed in oats and soybeans and some egress from roots to solution occurred. Picloram uptake by both plant species was markedly diminished with an increase in pH from 3.5 to 4.5, but pH had little effect from 4.5 to 9.5. Less picloram was taken up by oats and soybean roots from solution maintained at 4 C than at 26 C. Translocation to tops followed a similar trend. Increasing concentrations of three metabolic inhibitors, 2,4-dinitrophenol (DNP), sodium azide, and sodium arsenite, reduced root uptake of picloram in both species. All inhibitors (except DNP for oats) at 10−6to 10−5molar concentrations stimulated translocation of picloram to oats and soybean tops while higher concentrations depressed translocation.

Germination of macroconidia and growth of Sporidesmium sclerotivorum in vitro

1981 ◽  
Vol 27 (7) ◽  
pp. 664-669 ◽  
Author(s):  
W. A. Ayers ◽  
E. A. Barnett ◽  
P. B. Adams
Keyword(s):  
Organic Nitrogen ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Mineral Salts ◽  
Ethanolic Extracts ◽  
Carbon Substrates ◽  
Agar Media ◽  
Casamino Acids ◽  
Inorganic And Organic Nitrogen

Macroconidia of Sporidesmium sclerotivorum, a mycoparasite of Sclerotinia spp., were induced to germinte by aqueous and ethanolic extracts of sclerotia of Sclerotinia minor. Paper chromatography of sclerotial extracts indicated the presence of several amino acids and carbohydrates, chiefly glucose. Glucose was identified as the principal germination stimulant in ethanolic extracts. Glucose, fructose, mannose, cellobiose, sucrose, maltose, trehalose, soluble starch, and glycerol at 0.1% (w/v) stimulated macroconidia to germinate in 3–6 days at 25 °C. Crude sclerotial extracts, and glucose combined with inorganic and organic nitrogen sources, supported germination of greater numbers of macroconidia than glucose alone. Yeast extract, Casaminc acids, peptone, and several carbon substrates alone did not support germination. Macroconidia germinated well (> 30%) over the range of pH 3–7; maximum germination (> 80%) occurred at pH 5.0–5.5. Mycelial growth in a glucose – Casamino acids - mineral salts medium was also greatest in the range of pH 5.0–5.5, but growth fell off sharply below pH 4.5 and above pH 6.0. The fungus grew slowly on several complex agar media adjusted to pH 5.5.

scholarly journals Biodegradation of propiconazole by Pseudomonas putida isolated from tea rhizosphere

2009 ◽  
Vol 55 (No. 5) ◽  
pp. 196-201 ◽  
Author(s):  
S. Sarkar ◽  
S. Seenivasan ◽  
R. Premkumar
Keyword(s):  
Pseudomonas Putida ◽  
Ammonium Nitrate ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Nutritional Requirements ◽  
Mineral Salts ◽  
Tolerance Level ◽  
Triazole Fungicide ◽  
Growth Promoting

Biodegradation of triazole fungicide propiconazole was carried out <I>in vitro</I> by selected <I>Pseudomonas</I> strains isolated from tea rhizosphere. A total number of twelve strains were isolated and further screened based on their tolerance level to propiconazole. Four best strains were selected and further tested for their nutritional requirements. Among the different carbon sources tested glucose exhibited the highest growth promoting capacity and among nitrogen sources ammonium nitrate supported the growth to the maximum. The four selected <I>Pseudomonas</I> strains exhibited a range of degradation capabilities. Mineral salts medium (MSM) amended with glucose provided better environment for degradation with the highest degradation potential in strain MPR 4 followed by MPR 12 (72.8% and 67.8%, respectively).

Biochemical transformations of herbicide-derived anilines in culture medium and in soil

1972 ◽  
Vol 18 (12) ◽  
pp. 1857-1864 ◽  
Author(s):  
L. M. Bordeleau ◽  
R. Bartha
Keyword(s):  
Peroxidase Activity ◽  
Microbial Growth ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Geotrichum Candidum ◽  
Soil Extract ◽  
Breakdown Product ◽  
Mineral Salts ◽  
Carbon And Nitrogen

A correlation was established between peroxidase activity of soil and its capacity to transform 3,4-dichloroaniline, a breakdown product of several herbicides, to 3,3′,4,4′-tetrachloroazobenzene. Supplementation of soil by carbon and nitrogen sources for microbial growth stimulated both activities, and pointed to the microbial origin of soil peroxidases. Several peroxidase-producing bacteria, actinomycetes, and fungi were isolated from soil and were characterized. On the basis of its rapid growth and high peroxidase activity, a Geotrichum candidum strain was selected for further study. The culture filtrate of this organism exhibited both peroxidase and aniline oxidase activity. The highest per milligram dry weight activity of these enzymes was observed after cultivation on a mineral salts medium supplemented with soil extract and yeast extract.

Phylogenetic and physiological characterization of a “Nostocoida limicola”-like organism isolated from activated sludge

2002 ◽  
Vol 46 (1-2) ◽  
pp. 91-97 ◽  
Author(s):  
M. Schade ◽  
C. Beimfohr ◽  
H. Lemmer
Keyword(s):  
16S Rrna ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Industrial Effluent ◽  
Treatment Plant ◽  
Nitrogen Sources ◽  
Municipal Wastewater Treatment ◽  
Mineral Salts ◽  
16S Rrna Sequence ◽  
Specific Staining

Ten strains of “Nostocoida limicola”-like organisms were isolated from a municipal wastewater treatment plant with minor industrial effluent. The near complete 16S rDNA sequences were determined for four strains to analyze the position of the organisms within the phylogenetic tree. All four isolates showed the same 16S rRNA sequence and cluster within the green non sulfur bacteria. Hybridization with oligonucleotide probe AHW183 designed complementarily to diagnostic regions of the 16S rRNA sequences showed a positive reaction with all isolated strains. Hybridization of activated sludge samples with probe AHW183 indicates the filamentous “Nostocoida limicola”-like bacteria commonly to occur inside the sludge flocs and to hardly be detected within the flocs without a specific staining. On HA-medium all strains grow as long coiled, unbranched and unsheathed filaments which usually stain Gram positive and show a variable Neisser reaction. The isolates grow well in liquid HA-medium at COD values between 1 to 4 g O2 l−1 and a pH range from 6.0–7.8. No growth is detected in liquid R2A-medium and several synthetic mineral salts media containing various carbon and nitrogen sources.

Nutritional and environmental factors affecting growth and sporulation of Sporidesmium sclerotivorum

1981 ◽  
Vol 27 (7) ◽  
pp. 685-691 ◽  
Author(s):  
E. A. Barnett ◽  
W. A. Ayers
Keyword(s):  
Nitrogen Source ◽  
Agar Medium ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Mineral Salts ◽  
Factors Affecting ◽  
Carbon And Nitrogen ◽  
Naoh Solution ◽  
Casamino Acids ◽  
Nutritional Studies

Three of five isolates of Sporidesmium sclerotivorum, a mycoparasite of Sclerotinia spp., grew well on an agar medium containing mineral salts, glucose, thiamine, and glutamine or Casamino acids as the nitrogen source. The nitrogen requirement for two of the isolates was satisfied by NH4Cl, Casamino acids, or glutamine. Glutamine was the best single nitrogen source. Only one isolate, CS-1, was used in further nutritional studies. The optimum concentration of glutamine for growth was 5 g/L. Glucose, mannose, mannitol, and cellobiose were excellent carbon sources. A glucose concentration of 20 g/L was optimum. Mannitol supported greater growth than glucose with Casamino acids as the nitrogen source but glucose was the superior carbon source with glutamine as the nitrogen source. Greatest growth was achieved with a combination of these carbon and nitrogen sources. Sporidesmium sclerotivorum, isolate CS-1, required thiamine for growth and sporulation. Biotin stimulated growth. The fungus developed maximally within the range of pH 5.0–5.5 and growth was greatly reduced at a pH below 4.0 or above 6.0. Control of acidity by the periodic addition of NaOH solution permitted substantially increased growth. The optimum temperature for growth was 22.5–25.0 °C but production of macroconidia was greatest at 15–20 °C.

scholarly journals Physiological Conditions Conducive to High Cyanophycin Content in Biomass of Acinetobacter calcoaceticus Strain ADP1

2005 ◽  
Vol 71 (2) ◽  
pp. 858-866 ◽  
Author(s):  
Yasser Elbahloul ◽  
Martin Krehenbrink ◽  
Rudolf Reichelt ◽  
Alexander Steinbüchel
Keyword(s):  
Ammonium Sulfate ◽  
Incubation Temperature ◽  
Acinetobacter Calcoaceticus ◽  
Nitrogen Sources ◽  
Acid Extraction ◽  
Bacterial Cells ◽  
Mineral Salts ◽  
Average Molecular Mass ◽  
Thin Sections ◽  
Medium Components

ABSTRACT The effects of the inorganic medium components, the initial pH, the incubation temperature, the oxygen supply, the carbon-to-nitrogen ratio, and chloramphenicol on the synthesis of cyanophycin (CGP) by Acinetobacter calcoaceticus strain ADP1 were studied in a mineral salts medium containing sodium glutamate and ammonium sulfate as carbon and nitrogen sources, respectively. Variation of all these factors resulted in maximum CGP contents of only about 3.5% (wt/wt) of the cell dry matter (CDM), and phosphate depletion triggered CGP accumulation most substantially. However, addition of arginine to the medium as the sole carbon source for growth promoted CGP accumulation most strikingly. This effect was systematically studied, and an optimized phosphate-limited medium containing 75 mM arginine and 10 mM ammonium sulfate yielded a CGP content of 41.4% (wt/wt) of the CDM at 30°C. The CGP content of the cells was further increased to 46.0% (wt/wt) of the CDM by adding 2.5 μg of chloramphenicol per ml of medium in the accumulation phase. These contents are by far the highest CGP contents of bacterial cells ever reported. CGP was easily isolated from the cells by using an acid extraction method, and this CGP contained about equimolar amounts of aspartic acid and arginine and no detectable lysine; the molecular masses ranged from 21 to 29 kDa, and the average molecular mass was about 25 kDa. Transmission electron micrographs of thin sections of cells revealed large CGP granules that frequently had an irregular shape with protuberances at the surface and often severely deformed the cells. A cphI::ΩKm mutant of strain ADP1 with a disrupted putative cyanophycinase gene accumulated significantly less CGP than the wild type accumulated, although the cells expressed cyanophycin synthetase at about the same high level. It is possible that the intact CphI protein is involved in the release of CGP primer molecules from initially synthesized CGP. The resulting lower concentration of primer molecules could explain the observed low rate of accumulation at similar specific activities.

Cu(NO3)2-catalysed Direct Synthesis of 5-substituted 1H-tetrazoles from Alcohols or Aldehydes

2017 ◽  
Vol 41 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Chuanzhou Tao ◽  
Bin Wang ◽  
Lei Sun ◽  
Jiuyin Yi ◽  
Dahua Shi ◽  
...  
Keyword(s):  
Sodium Azide ◽  
Direct Synthesis ◽  
Nitrogen Sources ◽  
One Pot ◽  
Benzylic Alcohols ◽  
Substituted 1

A simple, convenient and practical protocol to synthesise 5-substituted 1 H-tetrazoles from alcohols or aldehydes is reported. Using ammonia and sodium azide as nitrogen sources and Cu(NO3)2 as catalyst, benzylic alcohols and benzaldehydes were directly converted into 5-substituted 1 H-tetrazoles in a one-pot procedure.

scholarly journals Consumption of nutritional supplements by gym attendants and health clubs

2021 ◽  
Vol 31 (Supplement_2) ◽  
Author(s):  
Daniel Figueiredo ◽  
Jorge Balteiro ◽  
Clara Rocha
Keyword(s):  
Statistical Treatment ◽  
Nutritional Supplements ◽  
Well Being ◽  
Cross Sectional Study ◽  
The Internet ◽  
Vitamin Supplement ◽  
Mineral Salts ◽  
Cross Sectional ◽  
Supplementation Frequency ◽  
Unbalanced Diet

Abstract Background Nowadays there has been a greater concern for health and well-being, as well as the constant search for the ‘ideal body’ trend. As a consequence, vitamin, protein and food supplements are more and more frequent in the population's dietary pattern, as compensation for an unbalanced diet and in the fight against intense physical/psychological wear and tear. The main objective of the study was to assess the prevalence of consumption of nutritional supplements by gym goers. Methods This is an observational, descriptive and cross-sectional study, lasting 9 months and with the collection of information through a questionnaire. The statistical treatment of the data was carried out with the aid of the SPSS program. Results It was found that the prevalence of consumption is higher in males (58.9%). The most consumed type of vitamin supplement were multivitamins with mineral salts (46.2%) and whey protein (89%) as a protein supplement. The most cited reason for its consumption was ‘accelerating the recovery’ (62.3%). The most common supplementation frequency is daily (54.1%), with the majority of respondents assuming a cost in the consumption of supplements in the range of 10 to 20 €monthly. The main place for its acquisition was the internet (65.6%) and the sources most mentioned by respondents to obtain knowledge about supplements were the internet and the trainer, both with 36.1%. Conclusions In Portugal, the prevalence of consumption is still unknown, and it is necessary to make the population aware of the possible risks associated with incorrect supplementation, unbalanced diet and exercise.

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