Efficiency of various bacterial suspensions derived from cecal floras of conventional chickens in reducing the population level of Salmonella typhimurium in gnotobiotic mice and chicken intestines

1985 ◽  
Vol 31 (9) ◽  
pp. 832-838 ◽  
Author(s):  
Sylvie Hudault ◽  
Hilaire Bewa ◽  
Chantal Bridonneau ◽  
Pierre Raibaud
Keyword(s):  
Salmonella Typhimurium ◽  
Volatile Fatty Acids ◽  
Anaerobic Bacteria ◽  
Population Level ◽  
Antagonistic Effect ◽  
Strictly Anaerobic ◽  
Antagonistic Bacteria ◽  
Fecal Flora ◽  
Intestinal Colonization ◽  
Gnotobiotic Mice

The antagonistic effect exerted towards Salmonella typhimurium by the flora issued from conventional chickens was studied in gnotobiotic animals. In germfree chickens and mice inoculated with S. typhimurium, the highest bacterial counts were observed in ceca, and were not significantly different in either host. The protection afforded by the inoculation of cecal flora issued from a conventional chicken was more effective when this flora was inoculated first into germfree chickens than when it was given only after inoculation with S. typhimurium. Administration of a cecal flora from a 15-day-old chick to gnotobiotic mice and chicken resulted in the inhibition of a further intestinal colonization by S. typhimurium in both hosts. Sixteen strains were isolated among the predominant populations of the fecal flora from chicken flora recipient mice. Association of 14 strains of strictly anaerobic bacteria with 2 strains of Escherichia coli and Streptococcus faecium only decreased the number of S. typhimurium in the ileum of gnotobiotic mice, but not in their cecum. Anaerobe cultures were obtained from 10−6 and 10−8 dilutions prepared from the fecal flora of gnotobiotic recipient mice. Antagonistic bacteria were present only in cultures from the 10−6 dilution. Cecal concentrations of volatile fatty acids were shown not to be the sole factor implicated in the antagonistic effect against S. typhimurium.

Antagonistic effect exerted by three strictly anaerobic strains against various strains of Clostridium perfringens in gnotobiotic rodent intestines

1987 ◽  
Vol 33 (3) ◽  
pp. 226-231 ◽  
Author(s):  
Nevzat Yurdusev ◽  
Jean Louis Nicolas ◽  
Monique Ladire ◽  
Robert Ducluzeau ◽  
Pierre Raibaud
Keyword(s):  
Clostridium Perfringens ◽  
Antagonistic Effect ◽  
Strictly Anaerobic ◽  
Bacterial Antagonism ◽  
Viable Cells ◽  
Large Numbers ◽  
Target Strain ◽  
Gnotobiotic Mice ◽  
Division Number

Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pathogenic B, C, and D C. perfringens serotypes. A drastic antagonistic effect of this three-strain association was also observed against a nonpathogenic C. perfringens serotype A (CpA). It was less efficient in gnotobiotic rats than in mice and less efficient in gnotobiotic mice fed an autoclaved diet than in mice fed the same diet sterilized by irradiation. No diffusible inhibitory substances against CpA were detected in feces of gnotobiotic mice harboring the three antagonistic strains, and no nutrient depletion was demonstrated in filtrates prepared from 10-fold diluted feces of these mice. In vitro mixed cultures of the three antagonistic strains failed to inhibit growth of CpA, whereas CpA did not multiply in a 10-fold diluted feces from gnotobiotic mice. A reverse correlation between the initial number of antagonistic strains and the division number of CpA was determined using serially diluted fecal suspensions. Thus, large numbers of viable cells of both antagonistic strains were required to inhibit the target strain in fecal suspensions as was also found in gnotobiotic mice intestines. However, no diffusible inhibitory substance was detectable nor could depletion of growth factors be identified as causing antagonism. Whatever factors that may be responsible for antagonism were found to be influenced by the host and its diet.

Inoculation of Broiler Chicks with a Continuous-Flow Derived Bacterial Culture Facilitates Early Cecal Bacterial Colonization and Increases Resistance to Salmonella typhimurium

1994 ◽  
Vol 57 (1) ◽  
pp. 12-15 ◽  
Author(s):  
DAVID J. NISBET ◽  
STEVEN C. RICKE ◽  
CHARLES M. SCANLAN ◽  
DONALD E. CORRIER ◽  
ALBERT G. HOLLISTER ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Propionic Acid ◽  
Continuous Flow ◽  
Bacterial Culture ◽  
Bacterial Colonization ◽  
Control Diet ◽  
Anaerobic Bacteria ◽  
Broiler Chicks ◽  
Strictly Anaerobic ◽  
Cecal Microflora

Experiments were conducted to examine the effect of a continuous-flow (CF) derived bacterial culture and of dietary lactose on colonization level by anaerobic bacteria in the ceca of broiler chicks. Cecal facultative and strictly anaerobic bacteria colony forming units (CFU), total volatile fatty acid (TVFA), lactic and propionic acid concentrations in 3-d-old chicks, and cecal log10 Salmonella typhimurium colonization and propionic acid concentrations in 10-d-old chicks were measured. Treatment groups were control diet, 2% lactose diet, CF culture + control diet, and CF culture + 2% lactose diet. Groups inoculated with CF culture had decreased (P < 0.05) Salmonella in cecal contents at 10 d of age. The level of Salmonella protection was significantly (P < 0.05) correlated with increased cecal bacteria CFU, TVFA, and propionic acid concentrations in 3-d-old chicks. Results indicated that inoculating newly hatched chicks with CF culture containing cecal microflora originally obtained from adult chickens, facilitates early cecal colonization by native cecal microflora, and this is associated with protection against Salmonella.

Role of Metabolic Intermediates in the Inhibition of Salmonella typhimurium and Salmonella enteritidis by Veillonella

1993 ◽  
Vol 56 (11) ◽  
pp. 932-937 ◽  
Author(s):  
ARTHUR HINTON ◽  
MICHAEL E. HUME ◽  
JOHN R. DELOACH
Keyword(s):  
Fatty Acids ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Volatile Fatty Acids ◽  
Agar Medium ◽  
Anaerobic Bacteria ◽  
Low Ph ◽  
Metabolic Intermediates ◽  
Lactate Medium

A Veillonella sp. was isolated from the cecal contents of adult chickens. The Veillonella was grown on an agar medium supplemented with 200 mM of lactate, pyruvate, fumarate, or succinate and adjusted to a pH of 6.7, 6.5, 6.3, 6.1, 5.9, or 5.7. No metabolites were added to the control media, but it was adjusted to the same pH levels as the supplemented media. The agar medium on which the Veillonella was grown was overlaid with fresh agar medium. Cultures of Salmonella typhimurium or Salmonella enteritidis were spread on the surface of the agar overlay, and the plates were incubated at 37°C for 14–18 h. Veillonella did not inhibit the growth of either salmonellae on any of the control or pyruvate medium. Veillonella did inhibit the growth of both salmonellae on lactate medium that had been adjusted to pH 6.3, 6.1, or 5.9 and on succinate medium that had been adjusted to pH 5.7. Veillonella also inhibited the growth of S. typhimurium on fumarate medium that had been adjusted to pH 6.7, 6.5, 6.3, 6.1, or 5.9; and it inhibited the growth of S. enteritidis on fumarate medium that had been adjusted to pH 6.7, 6.5, 6.3, or 6.1. Inhibition on lactate agar was correlated with the production of acetate and propionate by Veillonella and residual lactate in the medium; inhibition on fumarate agar was correlated with the production of propionate and lactate by Veillonella; and inhibition on succinate agar was correlated to the production of propionate at low pH levels. The findings indicate that anaerobic bacteria that produce these metabolic intermediates and anaerobic bacteria that can convert the intermediates to volatile fatty acids may be important components of probiotic cultures that can be provided to chicks to reduce colonization by salmonellae.

Isolation and identification of rumen bacteria capable of anaerobic rutin degradation

1969 ◽  
Vol 15 (12) ◽  
pp. 1365-1371 ◽  
Author(s):  
K. -J. Cheng ◽  
G. A. Jones ◽  
F. J. Simpson ◽  
M. P. Bryant
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Anaerobic Bacteria ◽  
Heterocyclic Ring ◽  
Ring Structure ◽  
Water Soluble ◽  
Rumen Bacteria ◽  
Isolation And Identification ◽  
Pure Cultures ◽  
Rutin Degradation

Fifteen strains of bacteria capable of degrading rutin anaerobically were isolated from bovine rumen contents and identified by morphological and biochemical evidence as strains of Butyrivibrio sp. Three cultures from a laboratory collection of 53 strains of rumen bacteria also used rutin anaerobically. Two, Butyrivibrio fibrisolvens D1 and Selenomonas ruminantium GA192, cleaved the glycosidic bond of rutin and fermented the sugar but did not degrade the insoluble aglycone produced; the third strain, Peptostreptococcus sp. B178, degraded the substrate to soluble products. Butyrivibrio sp. C3 degraded rutin, quercitrin, and naringin to water-soluble products, showing that the organism cleaved the heterocyclic ring of these compounds. Butyrivibrio sp. C3 fermented the sugar moiety of hesperidin but did not cleave the heterocyclic ring. It did not attack quercetin, taxifolin, protocatechuic acid, or phloroglucinol. In a medium containing rumen fluid, Butyrivibrio sp. C3 degraded rutin more than twice as fast as it did in a medium containing enzymatic casein hydrolyzate, volatile fatty acids, yeast extract, and hemin in place of rumen fluid.The observations reported in this paper are believed to represent the first recorded demonstration of degradation of the heterocyclic ring structure of rutin and other bioflavonoids in pure cultures of anaerobic bacteria.

scholarly journals Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
M. E. Ali ◽  
A. A. Khan ◽  
A. M. Akanda ◽  
Md. Kamal Uddin ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Control Agent ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Antagonistic Bacteria ◽  
And Storage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

Iodothyronine sulfate-hydrolyzing anaerobic bacteria isolated from human fecal flora

1985 ◽  
Vol 30 (3) ◽  
pp. 347-351
Author(s):  
W DEHERDER ◽  
M HAZENBERG ◽  
A PENNOCKSCHRODER ◽  
T VISSER
Keyword(s):  
Anaerobic Bacteria ◽  
Fecal Flora

scholarly journals Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens

2019 ◽  
Vol 64 (No. 9) ◽  
pp. 387-394
Author(s):  
Roman Svejstil ◽  
Vladimir Plachy ◽  
Miroslav Joch ◽  
Hana Salmonova ◽  
Dagmar Duskova ◽  
...  
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Volatile Fatty Acids ◽  
Animal Feed ◽  
Anaerobic Bacteria ◽  
Clostridium Butyricum ◽  
Feed Additive ◽  
Plate Count ◽  
The European Union ◽  
Plate Count Method

Clostridium butyricum CBM 588 is used as a probiotic in eastern Asian countries and has been recently approved as an animal feed additive in the European Union. The purpose of this study was to evaluate the effect of C. butyricum MIYAIRI 588 (CBM 588) on abundance of selected genera of caecal and crop bacteria, volatile fatty acids and growth performance of broiler chickens. We studied counts of anaerobic bacteria in caeca and crops of broiler chickens by plate-count method and evaluated their growth performance. CBM 588 significantly reduced E. coli counts in caeca of broiler chickens at days 10 and 42 and also enhanced their growth performance. Additionally, it significantly increased the amount of butyrate in the caeca that provides energy to enterocytes, resulting in increased weight gains. Out of the obtained results we conclude that C. butyricum CBM 588 influences caecal microbiota of broiler chickens and positively affects their growth performance.

scholarly journals How the Anaerobic Enteropathogen Clostridioides difficile Tolerates Low O2 Tensions

mBio ◽  
2020 ◽  
Vol 11 (5) ◽  
Author(s):  
Nicolas Kint ◽  
Carolina Alves Feliciano ◽  
Maria C. Martins ◽  
Claire Morvan ◽  
Susana F. Fernandes ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Anaerobic Bacteria ◽  
Growth Defect ◽  
Strictly Anaerobic ◽  
Low Oxygen ◽  
Air Exposure ◽  
Vegetative Cells ◽  
Content Type ◽  
Clostridioides Difficile

ABSTRACT Clostridioides difficile is a major cause of diarrhea associated with antibiotherapy. After germination of C. difficile spores in the small intestine, vegetative cells are exposed to low oxygen (O2) tensions. While considered strictly anaerobic, C. difficile is able to grow in nonstrict anaerobic conditions (1 to 3% O2) and tolerates brief air exposure indicating that this bacterium harbors an arsenal of proteins involved in O2 detoxification and/or protection. Tolerance of C. difficile to low O2 tensions requires the presence of the alternative sigma factor, σB, involved in the general stress response. Among the genes positively controlled by σB, four encode proteins likely involved in O2 detoxification: two flavodiiron proteins (FdpA and FdpF) and two reverse rubrerythrins (revRbr1 and revRbr2). As previously observed for FdpF, we showed that both purified revRbr1 and revRbr2 harbor NADH-linked O2- and H2O2-reductase activities in vitro, while purified FdpA mainly acts as an O2-reductase. The growth of a fdpA mutant is affected at 0.4% O2, while inactivation of both revRbrs leads to a growth defect above 0.1% O2. O2-reductase activities of these different proteins are additive since the quadruple mutant displays a stronger phenotype when exposed to low O2 tensions compared to the triple mutants. Our results demonstrate a key role for revRbrs, FdpF, and FdpA proteins in the ability of C. difficile to grow in the presence of physiological O2 tensions such as those encountered in the colon. IMPORTANCE Although the gastrointestinal tract is regarded as mainly anoxic, low O2 tension is present in the gut and tends to increase following antibiotic-induced disruption of the host microbiota. Two decreasing O2 gradients are observed, a longitudinal one from the small to the large intestine and a second one from the intestinal epithelium toward the colon lumen. Thus, O2 concentration fluctuations within the gastrointestinal tract are a challenge for anaerobic bacteria such as C. difficile. This enteropathogen has developed efficient strategies to detoxify O2. In this work, we identified reverse rubrerythrins and flavodiiron proteins as key actors for O2 tolerance in C. difficile. These enzymes are responsible for the reduction of O2 protecting C. difficile vegetative cells from associated damages. Original and complex detoxification pathways involving O2-reductases are crucial in the ability of C. difficile to tolerate O2 and survive to O2 concentrations encountered in the gastrointestinal tract.

An Anaerobic Process for Treatment of CTMP Effluent

1988 ◽  
Vol 20 (1) ◽  
pp. 143-147 ◽  
Author(s):  
T. Welander
Keyword(s):  
Volatile Fatty Acids ◽  
Anaerobic Bacteria ◽  
Methanogenic Bacteria ◽  
Second Stage ◽  
The Third ◽  
Multi Stage ◽  
Third Stage ◽  
Aluminum Iron ◽  
Stage Process ◽  
Iron And Calcium

A multi-stage process for treatment of CTMP effluent has been developed. It comprises primary settling and four biological stages. The concentration of hydrogen peroxide, a compound which is toxic to anaerobic bacteria, is reduced in the first biological stage by means of the biocatalytic action of biomass that is recycled from the following acidogenic and/or aerobic stages. The second stage is an acidogenic stage, in which volatile fatty acids are formed and remaining peroxide is decomposed. A mixture of aluminum, iron and calcium salts is added to the effluent in order to detoxify compounds which are toxic to methanogenic bacteria. The main part of the COD and BOD removal takes place in the third stage, the methanogenic stage, after which follows an aerobic stage for polishing and removal of bad-smelling compounds. The COD and BOD7 removals in the anaerobic part of the process are 60 and 90 %, respectively, and the methane yield is 0.20-0.25 Nm3/kg COD removed.

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