Targeted engineering of Cupriavidus necator chromosome for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil

2008 ◽  
Vol 86 (6) ◽  
pp. 621-627 ◽  
Author(s):  
Jun Mifune ◽  
Satoshi Nakamura ◽  
Toshiaki Fukui
Keyword(s):  
Soybean Oil ◽  
Vegetable Oil ◽  
Sole Carbon Source ◽  
Enzyme Assay ◽  
Wild Strain ◽  
Cupriavidus Necator ◽  
Pha Synthase ◽  
High Productivity ◽  
Aeromonas Caviae ◽  
Coa Reductase

Previous studies have demonstrated that heterologous expression of PHA synthase from Aeromonas caviae (PhaCAc), capable of accepting (R)-3-hydroxyacyl-CoA of C4–C7 as substrates, could confer the ability to PHA-negative mutant of Cupriavidus necator PHB-4 to synthesize poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) [P(3HB-co-3HHx)] from vegetable oils. The mutation point within pha operon in PHB-4 was determined to be a single nonsense mutation within the PHA synthase gene (phaCCn), suggesting the much lower β-ketothiolase and NADPH-dependent acetoacetyl-CoA reductase activities observed in this strain would be a polar effect of the mutation. For further efficient biosynthesis of P(3HB-co-3HHx) copolyester, C. necator wild strain H16 was engineered by homologous recombination targeting the chromosomal phaCCn, and the PHA productivity was compared with previous PHB–4-derived strain harboring phaCAc on a multi-copy plasmid (PHB–4/pJRDEE32d13). A strain H16CAc, in which phaCCn was substituted for phaCAc on the chromosome, could produce P(3HB-co-3HHx) from soybean oil with high productivity, but the 3HHx fraction in the accumulated polymer was decreased. Meanwhile, H16ΔC/pJRDEE32d13, that lost region for the original synthase gene and expresses exochromosomal phaCAc, grew and accumulated PHA with similar properties to the PHB–4-derived strain. The results of enzyme assay suggested that low β-ketothiolase activity might be relevant for decrease of growth ability accompanied by increase of 3HHx composition when soybean oil was fed as a sole carbon source. Key words: poly(hydroxyalkanoates), poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), PHA synthase, Cupriavidus necator, vegetable oil.

Carbon source pulsed feeding to attain high yield and high productivity in poly(3-hydroxybutyrate) (PHB) production from soybean oil using Cupriavidus necator

2012 ◽  
Vol 34 (6) ◽  
pp. 1003-1007 ◽  
Author(s):  
José Geraldo da Cruz Pradella ◽  
Jaciane Lutz Ienczak ◽  
Cecília Romero Delgado ◽  
Marilda Keico Taciro
Keyword(s):  
Carbon Source ◽  
Soybean Oil ◽  
Cupriavidus Necator ◽  
High Yield ◽  
High Productivity ◽  
Phb Production

Feeding antioxidant vitamin and vegetable oils to broilers: vitamin E reduced negative effect of soybean oil on immune response and meat lipid oxidation

2018 ◽  
Vol 58 (10) ◽  
pp. 1829
Author(s):  
M. Mohiti-Asli ◽  
M. Ghanaatparast-Rashti
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Vitamin C ◽  
Soybean Oil ◽  
Lipid Oxidation ◽  
Vegetable Oil ◽  
Canola Oil ◽  
Cellular Response ◽  
Dietary Supplementation ◽  
Antioxidant Vitamin

This study investigated the effect of feeding vitamin E, vitamin C, and two sources of vegetable oil on immune response and meat quality of broilers. A total of 320 one-day-old chicks were used in a completely randomised design with eight treatments arranged as a 2 × 2 × 2 factorial with two levels of vitamin E (0 and 200 mg/kg), two levels of vitamin C (0 and 1000 mg/kg), and two sources of vegetable oil (soybean and canola). Dietary supplementation of either vitamin E or C increased (P < 0.05) secondary humoral response, whereas oil sources had no significant effect. Broilers fed soybean oil had lower cellular response to the phytohemagglutinin skin test than those fed canola oil in diet, and supplementation of vitamin E increased cellular immune response. However, fat, cholesterol and pH of meat were not affected by source of oil or antioxidants, lipid oxidation was higher (P < 0.05) in thigh and breast meat of broilers fed soybean oil than canola oil. Dietary supplementation of vitamin E decreased (P < 0.05) lipid oxidation in thigh and breast of broilers fed diet containing soybean oil, without any effect on meat oxidation of those fed canola oil. Dietary supplementation of vitamin C increased lipid oxidation in thigh meat of broilers (P < 0.05). It can be concluded that inclusion of soybean oil to the diet, compared with canola oil, increased need for antioxidant. Vitamin E had beneficial effects on immune response and reduced meat lipid oxidation; nonetheless future studies should explore the antioxidant effect of vitamin C in stored meat.

Quantification of vegetable oil in recycled paper

TAPPI Journal ◽  
2020 ◽  
Vol 19 (9) ◽  
pp. 473-477
Author(s):  
YAO NTIFAFA ◽  
MARIA SZAJDA-LAM ◽  
ASHOK GHOSH ◽  
PETER W. HART
Keyword(s):  
Soybean Oil ◽  
Vegetable Oil ◽  
Oil Content ◽  
Strength Loss ◽  
Gas Chromatography Mass Spectrometry ◽  
Paper Strength ◽  
High Concentration ◽  
Recycled Paper ◽  
Pyrolysis Gas ◽  
Cooking Process

Vegetable soybean oil is commonly used in cooking foods that are packaged in takeaway paper-board containers. Vegetable oil is hydrophobic, and in sufficiently high concentration, could interfere with interfiber bonding and result in paper strength loss. In order to quantify the effect of oil on the resulting paperboard strength, it is necessary to quantify the oil content in paper. A lab method was evaluated to determine the soybean oil content in paper. Handsheets were made with pulps previously treated with different proportions of vegetable oil. Pyrolysis gas chromatography-mass spectrometry (pyGCMS) was used to quantify the amount of oil left in the handsheets. The results revealed a strong correlation between the amount of oil applied to the initial pulp and the amount of oil left in the handsheets. In addition, the effect of vegetable oils on paper strength may be affected by the cooking process. Vegetable oil is known to degrade over time in the presence of oxygen, light, and temperature. The vegetable oil was put in an oven to imitate the oil lifecycle during a typical pizza cooking process. The cooked oil was then left at room temperature and not protected from air (oxygen) or from normal daylight. The heated, then cooled, oil was stored over a period of 13 weeks. During this time, samples of the aged oil were tested as part of a time-based degradation study of the cooked and cooled oil.

scholarly journals Optimization of Microwave-Assisted Extraction of Residual Soybean Oil from Spent Bleaching Earth

2021 ◽  
Vol 302 ◽  
pp. 01009
Author(s):  
Chanatip Dejkajorn ◽  
Panawan Suttiarporn ◽  
Hussanai Sukkathanyawat ◽  
Kittisak Wichianwat ◽  
Saichon Sriphan ◽  
...  
Keyword(s):  
Soybean Oil ◽  
Vegetable Oil ◽  
Regression Coefficient ◽  
Bleaching Earth ◽  
Extraction Yield ◽  
Microwave Assisted Extraction ◽  
Ethanol Mixture ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Edible Vegetable Oil

Spent bleaching earth (SBE) which is generated from bleaching process is a valuable industrial waste of edible vegetable oil production because of residual edible vegetable oil absorbed. The residual oil in spent bleaching earth can be recovered and reused for application in the industries such as the production of biodiesel and lubricant. Currently, microwave-assisted extraction (MAE) technique is widely used because this method has a shorter extraction time and less solvent consumption when compared with traditional methods. In this study, MAE combined with solvent reflux was optimized using solvent screening experiments and response surface methodology (RSM) to obtain the highest yield of MAE extraction of residual soybean oil from spent bleaching earth. The extraction yield of residual soybean oil obtained from selected solvent were hexane-ethanol mixture (2:1 v/v, 10.19%) > hexane-ethanol mixture (1:1 v/v, 10.00%) >hexane-ethanol mixture (1:2 v/v, 9.98%) > hexane-ethanol mixture (1:3 v/v, 9.83%) > hexane-ethanol mixture (3:1 v/v, 8.59%) > hexane (8.17%) > acetone (7.73%). The regression coefficient (R-squared = 0.9852) expresses the accuracy of the regression and indicates the relationship between experimental data and predicted result, with high regression coefficient close to 1, which is remarkably desired. The experiment conditions for optimal MAE extraction of residual soybean oil from SBE were hexane-ethanol mixture (2:1 v/v), liquid to solid ratio 15.56:1 mL/g, extraction time 12.22 min, and microwave power 350 W. Under such conditions, the highest predicted value of the extraction yield of residual soybean oil was 10.43%.

scholarly journals Biosynthesis of Polyhydroxyalkanoate Terpolymer from Methanol via the Reverse β-Oxidation Pathway in the Presence of Lanthanide

2022 ◽  
Vol 10 (1) ◽  
pp. 184
Author(s):  
Izumi Orita ◽  
Gento Unno ◽  
Risa Kato ◽  
Toshiaki Fukui
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Ralstonia Eutropha ◽  
Negative Impact ◽  
Value Added ◽  
Pha Synthase ◽  
Growth Impairment ◽  
Oxidation Pathway ◽  
Methylotrophic Growth ◽  
Value Added Products

Methylorubrum extorquens AM1 is the attractive platform for the production of value-added products from methanol. We previously demonstrated that M. extorquens equipped with PHA synthase with broad substrate specificity synthesized polyhydroxyalkanoates (PHAs) composed of (R)-3-hydroxybutyrate and small fraction of (R)-3-hydroxyvalerate (3HV) and (R)-3-hydroxyhexanoate (3HHx) units on methanol. This study further engineered M. extorquens for biosynthesis of PHAs with higher 3HV and 3HHx composition focusing on the EMC pathway involved in C1 assimilation. The introduction of ethylmalonyl-CoA decarboxylase, catalyzing a backward reaction in the EMC pathway, aiming to increase intracellular propionyl/butyryl-CoA precursors did not affect PHA composition. Reverse b-oxidation pathway and subsequent (R)-specific hydration of 2-enoyl-CoA were then enhanced by heterologous expression of four genes derived from Ralstonia eutropha for the conversion of propionyl/butyryl-CoAs to the corresponding (R)-3-hydroxyacyl-CoA monomers. The resulting strains produced PHAs with higher 3HV and 3HHx compositions, while the methylotrophic growth was severely impaired. This growth impairment was interestingly restored by the addition of La3+ without a negative impact on PHA biosynthesis, suggesting the activation of the EMC pathway by La3+. The engineered M. extorquens synthesized PHA terpolymer composed of 5.4 mol% 3HV and 0.9% of 3HHx with 41% content from methanol as a sole carbon source in the presence of La3+.

Evaluation of natural and epoxidized vegetable oil in elastomeric compositions for tread rubber

2021 ◽  
pp. 009524432110386
Author(s):  
Camila Taliotto Scarton ◽  
Nayrim Brizuela Guerra ◽  
Marcelo Giovanela ◽  
Suélen Moresco ◽  
Janaina da Silva Crespo
Keyword(s):  
Mechanical Properties ◽  
Stearic Acid ◽  
Natural Rubber ◽  
Soybean Oil ◽  
Vegetable Oil ◽  
Epoxidized Soybean Oil ◽  
Natural State ◽  
Vegetable Soybean ◽  
Tire Industry ◽  
Petroleum Derivatives

In the tire industry, the incorporation of natural origin oils in the development of elastomeric formulations has been one of the alternatives to reduce the use of petroleum derivatives, with a high content of toxic compounds. In this work, soybean vegetable oil was investigated as a lubricant and co-activator in sulfur-vulcanized natural rubber compounds. The soybean oil was used in its natural state and chemically modified by the epoxy ring’s introduction in its structure. In an internal mixer a standard formulation of natural rubber, five formulations replacing a conventional aromatic oil and stearic acid by vegetable oil, and a formulation without an activation system were prepared. The natural and epoxidized soybean oil was characterized chemically, and the elastomeric compositions were evaluated by mechanical and rheological analysis. The mechanical properties showed satisfactory results when vegetable soybean oil was used as a lubricant and could be a substitute for conventional aromatic oils, thus guaranteeing reduction of aromatic polycyclic content in the formulations. The crosslink degree and the rheological characteristics of the samples prepared with vegetable soybean oil were similar to the natural rubber standard sample. The formulations without the zinc oxide and stearic acid evidenced the need for activators in the vulcanization reaction, as they presented properties below standard. We verified that the epoxidized soybean oil, even when promoting better dispersion of the fillers, interfered in the crosslink formation, and consequently there was a decrease in the mechanical properties of these formulations. Finally, we indicated vegetable soybean oil as a substitute for aromatic oil and stearic acid, in the elastomeric compositions used to manufacture treads.

scholarly journals Comparative metabolomics of Phialemonium curvatum as an omnipotent fungus cultivated on crude palm oil versus glucose

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Arief Izzairy Zamani ◽  
Susann Barig ◽  
Sarah Ibrahim ◽  
Hirzun Mohd. Yusof ◽  
Julia Ibrahim ◽  
...  
Keyword(s):  
Carbon Source ◽  
Organic Acids ◽  
Vegetable Oil ◽  
Carbon Metabolism ◽  
Sole Carbon Source ◽  
Carbon Sources ◽  
Tca Cycle ◽  
Central Carbon Metabolism ◽  
Targeted Metabolomics ◽  
Central Carbon

Abstract Background Sugars and triglycerides are common carbon sources for microorganisms. Nonetheless, a systematic comparative interpretation of metabolic changes upon vegetable oil or glucose as sole carbon source is still lacking. Selected fungi that can grow in acidic mineral salt media (MSM) with vegetable oil had been identified recently. Hence, this study aimed to investigate the overall metabolite changes of an omnipotent fungus and to reveal changes at central carbon metabolism corresponding to both carbon sources. Results Targeted and non-targeted metabolomics for both polar and semi-polar metabolites of Phialemonium curvatum AWO2 (DSM 23903) cultivated in MSM with palm oil (MSM-P) or glucose (MSM-G) as carbon sources were obtained. Targeted metabolomics on central carbon metabolism of tricarboxylic acid (TCA) cycle and glyoxylate cycle were analysed using LC–MS/MS-TripleQ and GC–MS, while untargeted metabolite profiling was performed using LC–MS/MS-QTOF followed by multivariate analysis. Targeted metabolomics analysis showed that glyoxylate pathway and TCA cycle were recruited at central carbon metabolism for triglyceride and glucose catabolism, respectively. Significant differences in organic acids concentration of about 4- to 8-fold were observed for citric acid, succinic acid, malic acid, and oxaloacetic acid. Correlation of organic acids concentration and key enzymes involved in the central carbon metabolism was further determined by enzymatic assays. On the other hand, the untargeted profiling revealed seven metabolites undergoing significant changes between MSM-P and MSM-G cultures. Conclusions Overall, this study has provided insights on the understanding on the effect of triglycerides and sugar as carbon source in fungi global metabolic pathway, which might become important for future optimization of carbon flux engineering in fungi to improve organic acids production when vegetable oil is applied as the sole carbon source.

Production of Polyhydroxyalkanoates Copolymers by Recombinant Pseudomonas in Plasmid- and Antibiotic-Free Cultures

2018 ◽  
Vol 28 (5) ◽  
pp. 225-235
Author(s):  
Edmar Ramos Oliveira-Filho ◽  
Linda P. Guamán ◽  
Thatiane Teixeira Mendonça ◽  
Paul F. Long ◽  
Marilda Keico Taciro ◽  
...  
Keyword(s):  
Carbon Source ◽  
Aeromonas Hydrophila ◽  
Sole Carbon Source ◽  
Ralstonia Eutropha ◽  
Pha Synthase ◽  
Medium Chain Length ◽  
Marker Free ◽  
Pha Copolymers ◽  
First Time ◽  
Induced Mutant

Three different polyhydroxyalkanoate (PHA) synthase genes (<i>Ralstonia eutropha</i> H16, <i>Aeromonas</i> sp. TSM81 or <i>Aeromonas hydrophila</i> ATCC7966 <i>phaC</i>) were introduced into the chromosome of two <i>Pseudomonas</i> strains: a native medium-chain-length 3-polyhydroxyalkanoate (PHA<sub>MCL</sub>) producer (<i>Pseudomonas</i> sp. LFM046) and a UV-induced mutant strain unable to produce PHA (<i>Pseudomonas</i> sp. LFM461). We reported for the first time the insertion of a chromosomal copy of <i>phaC</i> using the transposon system mini-Tn<i>7</i>. Stable antibiotic marker-free and plasmid-free recombinants were obtained. Subsequently, P(3HB-<i>co</i>-3HA<sub>MCL</sub>) was produced by these recombinants using glucose as the sole carbon source, without the need for co-substrates and under antibiotic-free conditions. A recombinant harboring <i>A. hydrophila phaC</i> produced a terpolyester composed of 84.2 mol% of 3-hydroxybutyrate, 6.3 mol% of 3-hydroxyhexanoate, and 9.5 mol% of 3-hydroxydecanoate from only glucose. Hence, we were successful in increasing the industrial potential of <i>Pseudomonas</i> sp. LFM461 strain by producing PHA copolymers containing 3HB and 3HA<sub>MCL</sub> using an unrelated carbon source, for the first time in a plasmid- and antibiotic-free bioprocess.

scholarly journals Draft Genome Sequence of a Chlorinated-Ethene Degrader,Cupriavidus necatorStrain PHE3-6 (NBRC 110655)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kenta Yonezuka ◽  
Jun Shimodaira ◽  
Michiro Tabata ◽  
Shun Nagase ◽  
Daisuke Kasai ◽  
...  
Keyword(s):  
Carbon Source ◽  
Genome Sequence ◽  
Sole Carbon Source ◽  
Draft Genome ◽  
Cupriavidus Necator ◽  
Chlorinated Ethenes ◽  
Draft Genome Sequence ◽  
Chlorinated Ethene

Cupriavidus necatorstrain PHE3-6 grows on phenol as a sole carbon source and cometabolizescis-andtrans-dichloroethenes and trichloroethene. Here, we report the draft genome sequence of PHE3-6, which provides insights into the degradation system of phenol and chlorinated ethenes.

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