Functional integrity and structural stability of freeze-dried ectomycorrhizal fungi established through viability assays

2001 ◽  
Vol 47 (2) ◽  
pp. 172-177 ◽  
Author(s):  
S Krishna Sundari ◽  
Alok Adholeya
Keyword(s):  
Structural Stability ◽  
Ectomycorrhizal Fungi ◽  
Total Protein ◽  
Total Protein Content ◽  
Total Biomass ◽  
Functional Integrity ◽  
Physical Integrity ◽  
Intraspecific Variations ◽  
Freeze Dried

Lyophilized vegetative mycelium of ectomycorrhizal fungi was subjected to various viability tests to confirm functional integrity. Physical integrity of freeze-dried cultures was comparable to that of non-lyophilized cultures. Inter- and intraspecific variations in morphology, physiology, and metabolic rate were maintained after lyophilization. Maintenance of total protein content confirmed metabolic stability. According to the assays of viability, a plating assay and determination of total biomass confirmed stable mitotic activity of the freeze-dried cultures.Key words: lyophilization, physical integrity, structural stability, total protein, total biomass.

Retardation Signal for Fluorescent Determination of Total Protein Content via Rapid and Sensitive Chip Moving Reaction Boundary Electrophoretic Titration

2014 ◽  
Vol 86 (6) ◽  
pp. 2888-2894 ◽  
Author(s):  
Houyu Wang ◽  
Yongting Shi ◽  
Jian Yan ◽  
Jingyu Dong ◽  
Si Li ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content

Effect of harvest period on the protein content in sea buckthorn leaves

2003 ◽  
Vol 83 (2) ◽  
pp. 409-410 ◽  
Author(s):  
T. S. C. Li ◽  
D. Wardle
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Freeze Drying ◽  
Conversion Factor ◽  
Growing Season ◽  
Sea Buckthorn ◽  
Leaf Protein ◽  
Total Protein Content ◽  
Male And Female ◽  
Freeze Dried

A study was conducted at Summerland, BC, in 2000 and 2001 to investigate the effect of harvest period on the protein content of sea buckthorn leaves. Leaves of both male and female plants were collected bi-weekly during the growing season and analyzed with a LECO FP-528 nitrogen analyzer after air- and freeze-drying. A conversion factor of kA = 5.7 was applied to obtain the protein content. Total protein content in sea buckthorn leaves showed very small differences between male and female plants and from either freeze-dried or air-dried materials. There were significant fluctuations among harvest periods in both years. Based on the results of this experiment, sea buckthorn leaves should be harvested from late July to early August since leaf protein content peaked around this period of time and started to decline significantly by the middle of August. Key words: Sea buckthorn, Hippophae rhamnoides L., leaf protein

scholarly journals Analysis of Fatty acid and Determination of Total Protein, Alkaloid, Saponin, Flavonoid of Bangladeshi Bombax ceiba Linn Leaves and Seeds

2016 ◽  
Vol 4 (04) ◽  
pp. 23-28
Author(s):  
Shahin Aziz ◽  
Shahal Ahmed ◽  
Sharmin Akter Lisa ◽  
Tanzima Parvin
Keyword(s):  
Fatty Acid ◽  
Protein Content ◽  
Total Protein ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Fatty Acid Analysis ◽  
Total Protein Content ◽  
Saponin Content ◽  
Kjeldahl Method

Bombax ceiba Linn belongs to the family of Bombacaceae and is an important medicinal plant. In Bangladesh, Bombax ceiba Linn is locally known as “Shimul tree”. The whole part of the plant used as traditional folk medicine for the treatment of antidysentric, anti diahorreal and antipyretic effects. The present communication attempts to evaluate fatty acid analysis by GC-MS spectrophotometer, total protein content by Kjeldahl method and to quantify some active constituents i.e. alkaloid, saponin and flavonoid. The fatty acid compositions of the petroleum ether extract of leaves and seeds of Bombax ceiba grown in Bangaladesh were determined by gas chromatography- mass spectrophotometer. 8 compounds were identified from leaves and 13 compounds were identified from the seeds. For both cases Palmitic acid showed higher value. The findings from present study showed the protein content for seeds have higher value (18.89%) than leaves of Bombax ceiba . The present investigation showed that both leaves and seeds of Bombax ceiba contain phytochemicals such as flavanoids, alakaloids and saponins in appreciable quantities. The flavonoid content of leaves was 5.97% and for the case of seeds (5.72%), the alkaloid content for leaves was (9.73%) and for seeds (31.44), the saponin content for the case of leaves (13.90%) and for the case of seeds was(43.58%).

scholarly journals Excipient suitability for freeze-dried protein solutions

2019 ◽  
Author(s):  
Giovanna Sevilha Ferreira ◽  
Laura de Oliveira Nascimento ◽  
Danilo Costa Geraldes
Keyword(s):  
Total Protein ◽  
Visual Inspection ◽  
Protein Solutions ◽  
Total Protein Content ◽  
Protein Formulations ◽  
Freeze Dried ◽  
Collapse Temperature ◽  
Sample Characterization ◽  
Model Protein ◽  
Simplex Lattice

This work focused on pre-formulation studies for freeze-dried protein formulations, using albumin as a model protein. Two aminoacids and two sugars were selected as lyoprotectors and studied according a mixture design (simplex-lattice). Sample characterization included total protein content, collapse temperature and visual inspection. Sugar formulations collapsed, but not aminoacids, which indicate non sugar excipients as the best choice under the studied conditions.

Determination of total protein content in soya-bean milk via visual moving reaction boundary titration

2013 ◽  
Vol 31 (11) ◽  
pp. 1064 ◽  
Author(s):  
Chengye GUO ◽  
Houyu WANG ◽  
Lei ZHANG ◽  
Liuyin FAN ◽  
Chengxi CAO
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Soya Bean ◽  
Total Protein Content

scholarly journals Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates

Talanta ◽  
2019 ◽  
Vol 198 ◽  
pp. 15-22 ◽  
Author(s):  
Pasquale Palladino ◽  
Alvaro Brittoli ◽  
Emanuela Pascale ◽  
Maria Minunni ◽  
Simona Scarano
Keyword(s):  
Protein Content ◽  
Protein Adsorption ◽  
Total Protein ◽  
Total Protein Content ◽  
Colorimetric Determination

scholarly journals Sexual dimorphism in the cerebrospinal fluid total protein content

2020 ◽  
Vol 58 (11) ◽  
pp. 1885-1890 ◽  
Author(s):  
Massimiliano Castellazzi ◽  
Stefano Pizzicotti ◽  
Ilenia Lombardo ◽  
Sarah Alfiero ◽  
Andrea Morotti ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
White Blood Cells ◽  
Total Protein Content ◽  
Laboratory Report ◽  
The Central Nervous System ◽  
Diagnostic Work ◽  
Work Up ◽  
Hospital Inpatients

AbstractObjectivesCerebrospinal fluid (CSF) is a clear, colorless body fluid filling the central nervous system. The determination of the CSF total protein (TP) content represents an important screening test of various pathologies. We aimed to address the effect of sex and age on CSF-TP content and the use of the current upper reference limits (URLs).MethodsCSF-TP content was analysed in a selected population of 1,252 patients (648 women and 604 men; age 18–89 years) who underwent lumbar puncture as a part of the diagnostic work-up. Samples presenting (i) more than 5 white blood cells (WBC)/µL, (ii) discolorations and (iii) reduced glucose were not included.ResultsThe CSF-TP content median values were significantly higher in men than in women (46 vs. 37 mg/dL) even after adjusting for age and different hospital inpatients. CSF-TP content positively correlated with age both in men and in women with a constant difference between sexes of 8.5 mg/dL. Applying the most used URLs (mainly 45 and 50 mg/dL, but also 60 mg/dL), men received a laboratory report suggestive of altered CSF-TP content more frequently than women. The use of age- and sex-calibrated CSF-TP URLs reduced, but not eliminated, this sex-gap.ConclusionsUsing the current URLs, a condition of “elevated CSF-TP content” may be overestimated in men or, conversely, underestimated in women, regardless of the age and of the diagnosis. These results highlighted the need to apply CSF-TP URLs values ​​normalized for both sex and age.

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