FATTY ACID COMPOSITION AND GLYCERIDE STRUCTURE IN RATS FED RAPESEED OIL OR CORN OIL

1963 ◽  
Vol 41 (1) ◽  
pp. 43-49 ◽  
Author(s):  
B. M. Craig ◽  
C. G. Youngs ◽  
Joyce L. Beare ◽  
J. A. Campbell

The fatty acid compositions of liver, skin, abdominal fat, and residual degutted carcass from each of five rats fed diets containing corn or rapeseed oil for 21 weeks were determined by gas-liquid chromatography. Statistical analysis of the results showed no significant variations among rats or among cutaneous, abdominal, and carcass fats. In these fat depots, the proportions of fatty acids resembled those of the diet except for palmitic, palmitoleic, oleic, and erucic acids. Glyceride compositions of the body fats were in agreement with those predicted by Vander Wal's distribution theory.

1963 ◽  
Vol 41 (1) ◽  
pp. 43-49 ◽  
Author(s):  
B. M. Craig ◽  
C. G. Youngs ◽  
Joyce L. Beare ◽  
J. A. Campbell

The fatty acid compositions of liver, skin, abdominal fat, and residual degutted carcass from each of five rats fed diets containing corn or rapeseed oil for 21 weeks were determined by gas-liquid chromatography. Statistical analysis of the results showed no significant variations among rats or among cutaneous, abdominal, and carcass fats. In these fat depots, the proportions of fatty acids resembled those of the diet except for palmitic, palmitoleic, oleic, and erucic acids. Glyceride compositions of the body fats were in agreement with those predicted by Vander Wal's distribution theory.


1961 ◽  
Vol 39 (12) ◽  
pp. 1855-1863 ◽  
Author(s):  
Joyce L. Beare

Fatty acids of liver, carcass, and milk of rats fed corn oil, rapeseed oil, partially hydrogenated herring oil, or margarine were examined by gas–liquid chromatography. Appreciable quantities of linoleic acid were maintained in the tissues and milk, even when the hydrogenated herring oil with a low level of linoleic acid was fed. The proportion of C20and C22acids deposited or secreted was related to that of the diet, and was highest with rapeseed oil. In the livers of rats fed each diet, long-chain, polyunsaturated acids were observed. The fatty acids of milk more closely reflected the dietary pattern than did those of the tissues.


1966 ◽  
Vol 44 (2) ◽  
pp. 179-185 ◽  
Author(s):  
B. L. Walker ◽  
V. F. Lishchenko

Lipids, extracted from the adrenals, brain, erythrocytes, heart, kidney, liver, plasma, and spleen of normal healthy female mink, were transesterified with 1% sulphuric acid in methanol, and the resulting methyl esters were analyzed by gas–liquid chromatography after purification by thin-layer chromatography. All of the tissues examined contained higher concentrations of unsaturated than of saturated acids, the highest levels of unsaturated acids occurring in the lipids of heart, adrenals, and plasma, and of the essential fatty acids (ω6 series, with six carbon atoms after last double bond) in plasma, erythrocyte, and kidney lipids. The fatty acid compositions of mink tissues resemble those reported in the literature for the rat; detailed comparisons are not possible because of the known influence of dietary factors on tissue fatty acids.


1968 ◽  
Vol 25 (11) ◽  
pp. 2419-2426 ◽  
Author(s):  
P. M. Jangaard ◽  
P. J. Ke

The fatty acid compositions of six samples of depot fat taken from harp seals (Pagophilus groenlandica), ranging in age from 1 to 2 days to adult, and a sample of milk lipids, were determined by gas–liquid chromatography (GLC). Three depot fat samples taken from hooded seals (Cystophora cristata), a sample of milk lipids from this species, and commercial seal oils from 4 years' production were similarly analyzed.No significant differences could be found between the composition of the milk lipids and the depot fat of the harp seals at the different ages, other than slightly lower iodine values in the younger animals (avg 140.6 vs. 148.9). The lipid samples from hooded seals had low iodine values (107–127) and correspondingly lower concentration of highly unsaturated fatty acids. The ratio of the fatty acids 22:5ω3/22:6ω3 could be used to differentiate between depot fat from harp seals (ratio 0.51–0.74) and that from hooded seals (ratio 0.25–0.42) in the samples analyzed.Commercial oils, which consist essentially of oil from harp seals, were similar in composition to oil prepared from this species alone.No difference could be found in the iodine values or fatty acid compositions of samples taken from various parts of the animal or from samples representing a cross section of blubber.


1964 ◽  
Vol 10 (3) ◽  
pp. 351-358 ◽  
Author(s):  
A. P. Tulloch ◽  
G. A. Ledingham

The fatty acid compositions of the oils of several spore forms of seven species of Gymnosporangium were determined by gas–liquid chromatography. No simple relationships were found between the host plants and the compositions of the spore oils. cis-9, 10-Epoxyoctadecanoic acid was found in the teliospore oil of five of the species. The oil of basidiospores closely resembled that of teliospores but there was considerable variation in the oil of aeciospores. In one species the aeciospore and teliospore oils were very similar to each other, in another species there was partial resemblance, and in a third species they were different. The fats from four "axenic" cultures of Gymnosporangium juniperi-virginianae Schw. were analyzed, and were quite different from those of any of the rust species but resembled those frequently obtained from the vegetative stages of many species of fungi.


2000 ◽  
Vol 38 (4) ◽  
pp. 1545-1551 ◽  
Author(s):  
Alexandre Leclercq ◽  
Annie Guiyoule ◽  
Mohamed El Lioui ◽  
Elisabeth Carniel ◽  
Jacques Decallonne

The cellular fatty acid compositions of 29 strains ofYersinia pestis representing the global diversity of this species have been analyzed by gas-liquid chromatography to investigate the extent of fatty acid polymorphism in this microorganism. After culture standardization, all Y. pestis strains studied displayed some major fatty acids, namely, the 12:0, 14:0, 3-OH-14:0, 16:0, 16:1ω9cis, 17:0-cyc, and 18:1ω9trans compounds. The fatty acid composition of the various isolates studied was extremely homogeneous (average Bousfield's coefficient, 0.94) and the subtle variations observed did not correlate with epidemiological and genetic characteristics of the strains. Y. pestis major fatty acid compounds were analogous to those found in other Yersiniaspecies. However, when the ratios for the 12:0/16:0 and 14:0/16:0 fatty acids were plotted together, the genus Yersinia could be separated into three clusters corresponding to (i) nonpathogenic strains and species of Yersinia, (ii) pathogenicYersinia enterocolitica isolates, and (iii) Yersinia pseudotuberculosis and Y. pestis strains. The grouping of the two latter species into the same cluster was also demonstrated by their high Bousfield's coefficients (average, 0.89). Therefore, our results indicate that the fatty acid composition ofY. pestis is highly homogeneous and very close to that ofY. pseudotuberculosis.


Author(s):  
O. V. Yakoviichuk ◽  
O. O. Danchenko ◽  
M. M. Danchenko ◽  
A. S. Fedorko ◽  
I. O. Kulyk

In science vicasol is known to be relative to the energy and antioxidant systems of tissues closely related to the biosynthesis and oxidation of fatty acids. This effect may cause changes in the fatty acid composition of tissues, and numerous works on the positive effect of quinones and their derivatives on the myocardial function, suggest that the proper dose and feeding can increase the stability and productivity of poultry. The given was aims to study the effect of vicasol on the fatty acid composition of goose myocardium. Myocardium was chosen as a biological object. Biological material was collected every 7 days throughout the period from the 21st to the 35th day of ontogeny, characterized by the state of physiological tension of geese. Feeding of geese with vicasol at a dose of 0.7 mg / kg body weight began with the 3rd day of ontogeny. Fatty acid analysis in myocardial tissues was performed by gas-liquid chromatography, pre-fabric samples were processed with the method by Palmer (1971) to obtain tissue lipid extracts. According to the results of the study, due to various changes in the content of the entire spectrum of fatty acids of the tissue during the experiment - the use of vicasol causes a slight increase in the unsaturation and the total content of unsaturated fatty acids in the myocardium of geese. These fluctuations are realized depending on the physiological state of the body. where vicasol can stimulate both the biosynthesis processes of individual fatty acids and their mitochondrial and microsomal oxidation, as evidenced by multidirectional reliable changes in the content of their entire spectrum. In particular, on the 21st day, the content of docosopentaenoic acid increased by 36.3% whereas the content of docosohexaenoic and linolenic acids decreased by an average of 21–24%, on the 28th day the content of eicosatetraic and docosahexaenoic acids increased whereas the content of the linoleic acids dropped by 22.6% in control groups. On the 35th day, the content of basic unsaturated fatty acids: palmitooleic, linoleic, linolenic and docosohexaenoic acids increased in the tissue under the influence of vicasol with complete depletion of docosopentaenoic acid. These fluctuations in fatty acid composition cause a slight increase in the total content of unsaturated fatty acids and increase the unsaturation of myocardial lipids on the 28th and 35th days of ontogeny of geese. Based on previous results regarding the antioxidant state of myocardium affected by vicasol and the given findings, which prove changes in the content of the entire spectrum of fatty acids during the selected ontogeny, vicasol can be used in poultry farming as a tool to improve the quality and the resilience of poultry.


1968 ◽  
Vol 25 (8) ◽  
pp. 1603-1620 ◽  
Author(s):  
R. G. Ackman ◽  
C. S. Tocher ◽  
J. McLachlan

Twelve species of marine unicellular algae have been cultured under comparable conditions and the total fatty acids determined by gas–liquid chromatography. Some specific fatty acid relationships paralleling taxonomic groupings were detected but generally individual fatty acids within various algal classes showed large relative variations. The four Bacillariophyceae examined were notable for fatty acid compositions giving iodine values <150, whereas in eight other classes the iodine values were all approximately 200. It is suggested that 5,8,11,14,17-eicosapentaenoic acid, found to be a common algal longer-chain polyunsaturated fatty acid, is characteristically deposited in the lipids of filter-feeders ingesting unicellular algae.


1968 ◽  
Vol 46 (2) ◽  
pp. 121-128 ◽  
Author(s):  
A. R. Neill ◽  
C. J. Masters

The lipids of the subcellular fractions of ovine heart homogenate have been separated by chromatography on silicic acid into cholesteryl ester, glyceride and free fatty acid, and phospholipid fractions. Gas–liquid chromatography was used to determine the fatty acid compositions of these fractions.In all the subcellular organelles, phospholipid was the major lipid class, but in the cell sap fraction, glycerides predominated.By means of this lipid fractionation, and the presence in this tissue of two unusual major components, it has been possible to demonstrate a differential distribution of fatty acids between the separate subcellular fractions. The significance of this finding has been discussed in relation to the functions of lipids, the processes of fatty acid desaturation in the cell, and membrane biochemistry.The phospholipids of ovine mitochondria have been shown to be markedly saturated by comparison with the properties of this fraction in other mammals, and the implications of this lack of polyunsaturated components are considered in the Discussion.


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