scholarly journals ЖИРНОКИСЛОТНИЙ СКЛАД МІОКАРДУ ГУСЕЙ ЗА ДІЇ ВІКАСОЛУ

Author(s):  
O. V. Yakoviichuk ◽  
O. O. Danchenko ◽  
M. M. Danchenko ◽  
A. S. Fedorko ◽  
I. O. Kulyk

In science vicasol is known to be relative to the energy and antioxidant systems of tissues closely related to the biosynthesis and oxidation of fatty acids. This effect may cause changes in the fatty acid composition of tissues, and numerous works on the positive effect of quinones and their derivatives on the myocardial function, suggest that the proper dose and feeding can increase the stability and productivity of poultry. The given was aims to study the effect of vicasol on the fatty acid composition of goose myocardium. Myocardium was chosen as a biological object. Biological material was collected every 7 days throughout the period from the 21st to the 35th day of ontogeny, characterized by the state of physiological tension of geese. Feeding of geese with vicasol at a dose of 0.7 mg / kg body weight began with the 3rd day of ontogeny. Fatty acid analysis in myocardial tissues was performed by gas-liquid chromatography, pre-fabric samples were processed with the method by Palmer (1971) to obtain tissue lipid extracts. According to the results of the study, due to various changes in the content of the entire spectrum of fatty acids of the tissue during the experiment - the use of vicasol causes a slight increase in the unsaturation and the total content of unsaturated fatty acids in the myocardium of geese. These fluctuations are realized depending on the physiological state of the body. where vicasol can stimulate both the biosynthesis processes of individual fatty acids and their mitochondrial and microsomal oxidation, as evidenced by multidirectional reliable changes in the content of their entire spectrum. In particular, on the 21st day, the content of docosopentaenoic acid increased by 36.3% whereas the content of docosohexaenoic and linolenic acids decreased by an average of 21–24%, on the 28th day the content of eicosatetraic and docosahexaenoic acids increased whereas the content of the linoleic acids dropped by 22.6% in control groups. On the 35th day, the content of basic unsaturated fatty acids: palmitooleic, linoleic, linolenic and docosohexaenoic acids increased in the tissue under the influence of vicasol with complete depletion of docosopentaenoic acid. These fluctuations in fatty acid composition cause a slight increase in the total content of unsaturated fatty acids and increase the unsaturation of myocardial lipids on the 28th and 35th days of ontogeny of geese. Based on previous results regarding the antioxidant state of myocardium affected by vicasol and the given findings, which prove changes in the content of the entire spectrum of fatty acids during the selected ontogeny, vicasol can be used in poultry farming as a tool to improve the quality and the resilience of poultry.

2020 ◽  
Vol 65 (No. 2) ◽  
pp. 51-57
Author(s):  
Hongtao Ren ◽  
Guang-Qin Zhang ◽  
Yong Huang ◽  
Xiao-Chan Gao

The effects of fatty acid composition in artificial feed on the change in the fatty acid composition of carp muscles and the relationship between Δ6-Fad and Elovl5 genes participating in the regulation of fatty acid synthesis were studied. Juveniles were fed three semi-purified diets (D1–D3) for 6 weeks with different lipid sources: D1, fish oil with high highly unsaturated fatty acids (HUFA); D2, corn oil with high linoleic acid (18:2n-6, LA), D3, linseed oil with high α-linolenic acid (18:3n-3, LNA); then, samples were taken to explore the molecular mechanism and the factors which affect the synthesis of carp HUFA. The content of LA and arachidonic acid (20:4n-6, AA) in common carp fed Diet 2 was higher than in carp receiving D3 (P < 0.05), but the contents of eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) were lower than in carp fed D1 and D2 (P < 0.05). The liver transcript abundance of Δ6-Fad and Elovl5 in fish fed D2 and D3 at the end of 6 weeks was generally higher than the abundance in the initial stage and in the fish fed D1 (P < 0.05). The results suggest that the common carp can biosynthesise HUFA, and the type and content of fatty acids in feed affected not only the composition and content of fatty acids in common carp muscles, but also the Δ6-Fad and Elovl5 gene expression involved in the biosynthesis of HUFA. Feeding high levels of n-3 HUFA diet can increase the body content of EPA and DHA in common carp. The results of this research may provide a theoretical basis for choosing an appropriate source of lipid for common carp feeds.  


1979 ◽  
Vol 42 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Ph. G. Pittet ◽  
D. Halliday ◽  
P. E. Bateman

1. Adipose tissue samples were obtained by needle biopsy from three subcutaneous sites (thigh, abdomen and upper arm) in twenty-two obese women. The fatty acid composition was determined using gas-liquid chromatography and the results presented relate to eleven component fatty acids.2. The fatty acid composition of adipose tissue obtained from the arm and abdomen was remarkably similar, with the exception of the levels of lauric acid.3. The analyses showed that the majority of the saturated fatty acids were present in smaller proportions whilist the majority of unsaturated fatty acids were present in larger proportions in the thigh than in the two other sites. Highly significant inter-site differences were demonstrated for six of the major fatty acids and also for both the total amounts of saturated and unsaturated fatty acids and their ratios.4. No marked differences in the fatty acid composition of adipose tissue from obese subjects were revealed during this study when compared with previously reported results obtained from ‘normal-weight’ subjects.


1970 ◽  
Vol 24 (2) ◽  
pp. 202-208 ◽  
Author(s):  
Mohammad Mizanur Rahman ◽  
Mohammad Shahjahan

Fatty acid composition of Nyctanthes arbortristis Linn root extracted successively  with light petroleum ether (40-600C bp.)- n-hexane and chloroform were analyzed by gas liquid chromatography (GLC). Twenty one fatty acids were identified in the Seuli root. The major saturated and unsaturated fatty acids are palmitic acid (C16:0, 13.97%) and oleic acid (C18:1, 28.43%). Physio-chemical characteristics, such as iodine value, moisture, ash, lignin and crude fibre of the Seuli root were also determined.   Keywords: Nyctanthes arbortristis Linn; Seuli; root; fatty acid composition; gas liquid chromatography. DOI: http://dx.doi.org/10.3329/jbcs.v24i2.9709 Journal of Bangladesh Chemical Society, Vol. 24(2), 202-208, 2011


Author(s):  
L.G. Kashirina ◽  
N.I. Morozova ◽  
K.A. Ivanischev ◽  
K.I. Romanov

Цель исследований определение влияния антиоксидантных препаратов Е-селен и Бутофан на продуктивность новотельных коров, состав молока, дисперсность молочного жира и жирнокислотный состав сливочного масла. Исследования были выполнены на 12 головах новотельных коров-аналогов черно-пестрой породы в возрасте трех лет, сформированных в три группы контрольную и две опытные по 4 головы в каждой в условиях ООО Заря Рязанского района Рязанской области. Дисперсность молочного жира определяли по количеству и диаметру молочных жировых шариков. Из молока готовили сладко-сливочное масло традиционным способом, в соответствии с действующим ГОСТом. Анализ масла на жирнокислотный состав проводили в ООО Московская независимая лаборатория качества сырья и пищевых продуктов , с использованием аппаратно-программного комплекса для медицинских исследований на базе хроматографа Хроматэк-Кристалл 5000 , с помощью программы Ехсеl . Определено влияние вышеуказанных препаратов на уровень продуктивности коров, состав молока, дисперсность молочного жира, жирнокислотный состав сливочного масла. При применении препаратов, обладающих антиоксидантной активностью, происходит торможение процессов перекисного окисления липидов в организме коров на разных стадиях лактации и свободные радикалы не накапливаются в организме. Наиболее выраженной активностью обладал препарат Бутофан , при его использовании изменялся дисперсный состав молочного жира в молоке в сторону увеличения количества жировых шариков с преобладанием крупной фракции. Это оказало положительное влияние на изменение состава жирных кислот в сливочном масле, на увеличение фракции ненасыщенных жирных кислот и улучшение качественных показателей сливочного масла.The purpose of the research is to determine the effect of antioxidant preparations E-selenium and Butofan on the productivity of newly calved cows, the composition of milk, the dispersion of milk fat and the fatty acid composition of butter. The studies were performed with 12 newly calved cows - analogues of a black-and-white breed at the age of 3 years old, formed in three groups - a control and two experimental ones having 4 animals each at LLC Zarya, Ryazan district of Ryazan region. The dispersion of milk fat was determined by the number and diameter of milk fat globules. Sweet butter was prepared from milk in the traditional way, in accordance with the current GOST. An analysis of the butter for the fatty acid composition was carried out at Moscow Independent Laboratory for the Quality of Raw Materials and Food Products, LLC, using the hardware-software complex for medical research, based on Chromatek-Crystal 5,000 chromatograph with the help of Excel program. The influence of the above preparations on the level of cow productivity, milk composition, dispersion of milk fat and fatty acid composition of butter was determined. When using preparations with antioxidant activity, processes of inhibition of lipid peroxidation processes in the body of cows at different stages of lactation occured and free radicals did not accumulate in the body. Butofan preparation had the most pronounced activity when used. The dispersed composition of milk fat in milk changed in the direction of increasing the number of fat globules with a predominance of a large fraction. This had some positive effect on the change in the composition of fatty acids in butter, on the increase in the fraction of unsaturated fatty acids and the improvement of the quality parameters of butter.


1970 ◽  
Vol 46 (1) ◽  
pp. 111-116
Author(s):  
BK Paul ◽  
MN Ahmed ◽  
GC Saha

Carum roxburghianum Benth. (Radhuni) seeds of three different places of Bangladesh were investigated to determine the fatty acid composition and physico-chemical properties of extracted oil. The seeds were found to contain oil ranging from 15.31 to 20.32%. The percentage compositions of fatty acids were identified and quantified by Gas Liquid Chromatography (GLC). The saturated and unsaturated fatty acids in extracts were 4.95 to 6.27% and 93.73 to 94.89%, respectively. Among six fatty acids identified in this study, oleic acid contributed the highest portion (76.44 to 79.16%), whereas, linoleic (1.38%), linolenic (15.28 - 15.40%), stearic (0.70%), palmitic (4.95 - 5.57%) and ecosenoic acid (0.51 - 15.73%) together contributed the rest. Physico-chemical properties of the oil extracted were also investigated. The specific gravity, refractive index, optical rotation were recorded as 0.903 to 0.918 at 30°C, 1.465 to 1.470 at 30°C and +8.54° to +9.56° at 26°C, respectively. The chemical properties like saponification value (183.52 - 193.45), iodine value (80.99 - 120.90), peroxide value (23.25 - 36.16), acid value (143.84 - 162.99), ester value (48.12 - 62.03), percentage of unsaponifiable matter (3.56 - 6.51%), Reicher-Meissel value (2.00 - 3.12), Polenske value (4.12 - 6.20) and Henher value (85.12 - 95.56) were determined. Overall Radhuni seeds oil can be considered as a good source of oleic acid. Keywords: Carum roxburghianum; Radhuni seed oil; fatty acid composition; oleic acid; Gas Liquid Chromatography. DOI: http://dx.doi.org/10.3329/bjsir.v46i1.8113 Bangladesh J. Sci. Ind. Res. 46(1), 111-116, 2011


2015 ◽  
Vol 38 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Daniela Belichovska ◽  
Zehra Hajrulai-Musliu ◽  
Risto Uzunov ◽  
Katerina Belichovska ◽  
Mila Arapcheska

Abstract Fatty acid composition of foods has a great impact on nutrition and health. Therefore, thе determination and knowledge of the fatty acid composition of food is very important for nutrition. Due to the high nutritional characteristics of ostrich meat and its products, the research determining their quality is of topical interest. The aim of the present investigation was the determination of fatty acid composition of ostrich adipose tissue. The content of fatty acids was determined according to AOAC Official Methods of Analysis and determination was performed using a gas chromatograph with a flame-ionization detector (GC-FID). The results are expressed as a percentage of the total content of fatty acids. The method was validated and whereupon the following parameters were determined: linearity, precision, recovery, limit of detection and limit of quantification. The repeatability was within of 0.99 to 2.15%, reproducibility from 2.01 to 4.57%, while recovery ranged from 94.89 to 101.03%. According to these results, this method is accurate and precise and can be used for analysis of fatty acids in foods. It was concluded that the content of saturated fatty acids (SFA) accounted 34.75%, of monounsaturated fatty acids (MUFA) 38.37%, of polyunsaturated fatty acids (PUFA) 26.88%, of total unsaturated fatty acids (UFA) 65.25% and of desirable fatty acids (DFA) (total unsaturated + stearic acid) 70.37% of the analysed samples. The ratio polyunsaturated/saturated fatty acids accounted 0.77. The most present fatty acid is the oleic (C18:1n9c) with 28.31%, followed by palmitic (C16:0) with 27.12% and linoleic (C18:2n6c) acid with 25.08%. Other fatty acids are contained in significantly lower quantities.


1997 ◽  
Vol 77 (1) ◽  
pp. 91-95 ◽  
Author(s):  
L. Velasco ◽  
J. M. Fernández-Martínez ◽  
A. De Haro

The improvement of oil composition for C18 unsaturated fatty acids is one of the most important breeding objectives for the development of high-quality Ethiopian mustard oil. The objective of this research was to induce variability for fatty acid composition in this species using chemical mutagenesis. Seeds of line C-101 were treated with 1% ethylmethane sulfonate and the progenies of 8331 M2 plants were screened for fatty acid composition of the oil with near infrared reflectance spectroscopy followed by gas liquid chromatography on selected plants. Oleic and linoleic desaturation ratios (ODR and LDR, respectively) were used to detect putative mutants with altered levels of C18 unsaturated fatty acids. Seven of these mutants were isolated and M4 lines were developed. Six lines were characterized by low ODR, showing an increase in oleic acid and a reduction in linoleic acid. Three of these lines also showed reduced levels of linolenic acid. The other mutant line showed a decrease in both LDR and linolenic acid content. Since different enzyme systems seemed to be altered, a further oil improvement for C18 unsaturated fatty acids may be achieved through recombination among mutants. Key words: Brassica carinata Braun, C18 unsaturated fatty acids, mutagenesis


Author(s):  
М.Н. ПАК ◽  
Р.В. ИВАНОВ ◽  
У.В. ХОМПОДОЕВА

Представлены результаты исследования питательных веществ и жирнокислотного состава сеяных и естественных травостоев, хвощовых и безхвощовых (разнотравных) пастбищ Якутии. Установлено, что в составе липидов овса посевного доминируют ненасыщенные жирные кислоты (56,59 %), у отавы естественного травостоя этот показатель почти 2 раза меньше (31,15 %). Хвощ пестрый также содержит значительное количество полиненасыщенных жирных кислот, особенно линолевой и арахидоновой. Хвощовое пастбище превосходит разнотравное по содержанию в растительности протеина, жира, золы и БЭВ, за исключением клетчатки. Полученные нами результаты свидетельствуют о необходимости широкого использования посевов овса и хвощовых пастбищ для нажировки табунных лошадей якутской породы. The article presents the results of the study of nutrients and fatty acid composition of seeded and natural grasslands, horsetail and horsetail-free pastures in Yakutia. It was found that unsaturated fatty acids dominate in the composition of oat lipids, the total content of which was 56.59 %, while the natural grass stand oats have almost 2 times less (31.15 %). Variegated horsetail contains a significant amount of polyunsaturated fatty acids, especially linoleic and arachidonic. Horsetail pasture is superior to mixed grass pasture in terms of protein, fat, ash and nitrogen-free extractive fraction, except fiber. The results that we obtained indicate the need for widespread use of oat crops and horsetail pastures in breeding herd horses of the Yakut breed.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 232-233
Author(s):  
Ekaterina Karpova ◽  
Ludmila Chizhova ◽  
Eugenia Surzhikova

Abstract The early period of postnatal ontogeny of sheep is characterized by a variety of metabolic processes that result in the formation and consolidation of a certain type of metabolism. A special role is played by lipids, which together with proteins and carbohydrates constitute the bulk of organic compounds, cells and the organism as a whole. The purpose of this research was to study the polymorphism of CAST and GH genes as well as its association with the fatty acid composition of blood plasma lipids in lambs. The content of fatty acids was determined by gas-liquid chromatography in the form of methyl esters. From the data obtained, it follows that in all studied ontogeny periods the peripheral blood of sheep with CASTNN and CASTMM genotypes had a higher concentration of fatty acids such as palmitic, stearic, linoleic, and arachidonic acids. The content is as follows: 27.58, 45.40, 3.16, 0.28% vs. 26.21, 45.31, 3.62, 0.18% at the age of 2 months; 25.07, 43.25, 6.05, 1.74% vs. 23.24, 38.76, 6.49, 1.32% at the age of 4 months; 20.64, 22.26, 14.17, 3.38% vs. 17.64, 19.91, 15.07, 2.46% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Studies of blood lipid composition of GHBBand GHAA genotype carriers showed that palmitic, stearic, linoleic, and arachidonic acids were dominant: 27.95, 27.96, 19.04% vs. 26.08, 23.88, 17.56% at the age of 2 months, 46.87, 39.87, 22.37% vs. 3.24, 6.87, 15.56% at the age of 4 months; 0.45, 1.68, 3.81% vs. 0.29, 1.29, 3.19% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Thus, this study emphasizes that there is a close relationship between the ratio of fatty acids in total blood lipids in lambs and the intensity of growth and development, ensuring and controlling the direction of biochemical processes in the growth and development of young sheep.


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