Experiments on Rouleau Formation

1973 ◽  
Vol 51 (9) ◽  
pp. 690-699 ◽  
Author(s):  
D. Kernick ◽  
A. W. L. Jay ◽  
S. Rowlands ◽  
L. Skibo
Keyword(s):  
Red Blood Cells ◽  
Erythrocyte Sedimentation Rate ◽  
Linear Function ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Temperature Dependent ◽  
Experimental Conditions ◽  
Erythrocyte Sedimentation ◽  
Rouleau Formation ◽  
High Erythrocyte Sedimentation Rate

Rouleau formation was measured by an adaptation of an old method used by Ponder (1927, Q. J. Exp. Physiol. 16, 173–194). Blood was depleted of red cells to a low hematocrit. It was stirred under standardized conditions, and after various times, samples were examined by microscope. Mean rouleau length was taken as an index of rouleau formation. The method was used to observe changes of rouleau formation with time and temperature and with variations in erythrocyte sedimentation rate (E.S.R.). Under these experimental conditions, mean rouleau length increases with time up to 40 min. Over a range of hematocrit values between 0 and 4%, rouleau length is a linear function of hematocrit. At higher hematocrits random aggregations based on columnar units are seen. Rouleau formation is temperature dependent and maximum mean rouleau length occurs around 37 °C. Subjects with a high erythrocyte sedimentation rate tend to form long rouleaux. By exchanging red blood cells (R.B.C.) between two ABO Rh compatible patients with greatly differing E.S.R. and mean rouleau length, it was shown that the agent responsible was in the plasma and not on the R.B.C. The results were compared with a theory of aggregation which proved inadequate in some respects.

Influence of cadmium on hematological indicators of carp

2020 ◽  
Vol 1 ◽  
pp. 92-96
Author(s):  
L. Yu. Karpenko ◽  
◽  
P. A. Polistovskaya ◽  
A. I. Enukashvili ◽  
A. B. Balykina ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Red Blood Cells ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Power Plants ◽  
Cadmium Acetate ◽  
Control Group ◽  
Erythrocyte Sedimentation ◽  
Short Period

The article is devoted to the assessment of the toxic effects of cadmium acetate on fish. The research topic is relevant, since heavy metals, and especially cadmium, are ones of the most dangerous pollutants for the aquatic environment. After the emission of the already used water by the electricity-power plants into the reservoirs of circulating wa-ter, a sharp increase in the maximum permit-ted concentrations of heavy substances can be observed often, which act on hydrobi-onts for a rather short period of time. That is why, modeling this effect is necessary to study the effects of heavy metals. The aim of the assay was to study the hematological parameters of carp after exposure to various concentrations of cadmium acetate. During the experiment, 5 groups of fish were formed - 1 control group (10 fish), 4 experimental groups - 10 fish each. The experimental groups of fish were exposed in a solution of cadmium acetate (Cd (CH3COO) 2) with concentrations of 0.05 mg / l, 0.5 mg / l, 5 mg / l, 50 mg / l (exceeding the maximum permissible concentration of cadmium for fisheries in 10, 100, 1000 and 10000 times respectively). We have investigated: the erythrocyte sedimentation rate, the number of red blood cells in the blood and the con-centration of hemoglobin in the blood. Dur-ing the study, the authors noted an increase in the erythrocyte sedimentation rate due to the formation of conglomerates of blood cells with plasma proteins, as well as a de-crease in the number of red blood cells due to the toxic effect of cadmium acetate. A decrease in the concentration of hemoglobin in the blood was also observed, probably associated with a decrease in the number of red blood cells due to their lysis. With an increase in the concentration of the metal under study, there is a significant increase in ESR, a decrease in the level of hemoglobin and the number of red blood cells.

scholarly journals The Erythrocyte Sedimentation Rate and Its Relation to Cell Shape and Rigidity of Red Blood Cells from Chorea-Acanthocytosis Patients in an Off-Label Treatment with Dasatinib

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 727
Author(s):  
Antonia Rabe ◽  
Alexander Kihm ◽  
Alexis Darras ◽  
Kevin Peikert ◽  
Greta Simionato ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Red Blood Cells ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Cell Shape ◽  
Healthy Controls ◽  
Off Label ◽  
Erythrocyte Sedimentation ◽  
Hematology Parameters

Background: Chorea-acanthocytosis (ChAc) is a rare hereditary neurodegenerative disease with deformed red blood cells (RBCs), so-called acanthocytes, as a typical marker of the disease. Erythrocyte sedimentation rate (ESR) was recently proposed as a diagnostic biomarker. To date, there is no treatment option for affected patients, but promising therapy candidates, such as dasatinib, a Lyn-kinase inhibitor, have been identified. Methods: RBCs of two ChAc patients during and after dasatinib treatment were characterized by the ESR, clinical hematology parameters and the 3D shape classification in stasis based on an artificial neural network. Furthermore, mathematical modeling was performed to understand the contribution of cell morphology and cell rigidity to the ESR. Microfluidic measurements were used to compare the RBC rigidity between ChAc patients and healthy controls. Results: The mechano-morphological characterization of RBCs from two ChAc patients in an off-label treatment with dasatinib revealed differences in the ESR and the acanthocyte count during and after the treatment period, which could not directly be related to each other. Clinical hematology parameters were in the normal range. Mathematical modeling indicated that RBC rigidity is more important for delayed ESR than cell shape. Microfluidic experiments confirmed a higher rigidity in the normocytes of ChAc patients compared to healthy controls. Conclusions: The results increase our understanding of the role of acanthocytes and their associated properties in the ESR, but the data are too sparse to answer the question of whether the ESR is a suitable biomarker for treatment success, whereas a correlation between hematological and neuronal phenotype is still subject to verification.

Multiple myeloma with serum IgM kappa and Bence Jones lambda biclonal gammopathy.

1986 ◽  
Vol 32 (12) ◽  
pp. 2220-2221 ◽  
Author(s):  
M S Graziani ◽  
U Lippi
Keyword(s):  
Multiple Myeloma ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Light Chains ◽  
Laboratory Studies ◽  
Rare Finding ◽  
Erythrocyte Sedimentation ◽  
Biclonal Gammopathy ◽  
High Erythrocyte Sedimentation Rate

Abstract We report a rare finding: IgM kappa and Bence Jones lambda double gammopathy in serum of a 80-year-old man with untreated symptomatic multiple myeloma. The unusual findings are confined to the laboratory studies demonstrating also a Bence Jones lambda proteinuria, high erythrocyte sedimentation rate (113 mm/h), and anemia. The synthesis of the different light chains seems to occur in separate cellular clones.

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