scholarly journals The Erythrocyte Sedimentation Rate and Its Relation to Cell Shape and Rigidity of Red Blood Cells from Chorea-Acanthocytosis Patients in an Off-Label Treatment with Dasatinib

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 727
Author(s):  
Antonia Rabe ◽  
Alexander Kihm ◽  
Alexis Darras ◽  
Kevin Peikert ◽  
Greta Simionato ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Red Blood Cells ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Cell Shape ◽  
Healthy Controls ◽  
Off Label ◽  
Erythrocyte Sedimentation ◽  
Hematology Parameters

Background: Chorea-acanthocytosis (ChAc) is a rare hereditary neurodegenerative disease with deformed red blood cells (RBCs), so-called acanthocytes, as a typical marker of the disease. Erythrocyte sedimentation rate (ESR) was recently proposed as a diagnostic biomarker. To date, there is no treatment option for affected patients, but promising therapy candidates, such as dasatinib, a Lyn-kinase inhibitor, have been identified. Methods: RBCs of two ChAc patients during and after dasatinib treatment were characterized by the ESR, clinical hematology parameters and the 3D shape classification in stasis based on an artificial neural network. Furthermore, mathematical modeling was performed to understand the contribution of cell morphology and cell rigidity to the ESR. Microfluidic measurements were used to compare the RBC rigidity between ChAc patients and healthy controls. Results: The mechano-morphological characterization of RBCs from two ChAc patients in an off-label treatment with dasatinib revealed differences in the ESR and the acanthocyte count during and after the treatment period, which could not directly be related to each other. Clinical hematology parameters were in the normal range. Mathematical modeling indicated that RBC rigidity is more important for delayed ESR than cell shape. Microfluidic experiments confirmed a higher rigidity in the normocytes of ChAc patients compared to healthy controls. Conclusions: The results increase our understanding of the role of acanthocytes and their associated properties in the ESR, but the data are too sparse to answer the question of whether the ESR is a suitable biomarker for treatment success, whereas a correlation between hematological and neuronal phenotype is still subject to verification.

Experiments on Rouleau Formation

1973 ◽  
Vol 51 (9) ◽  
pp. 690-699 ◽  
Author(s):  
D. Kernick ◽  
A. W. L. Jay ◽  
S. Rowlands ◽  
L. Skibo
Keyword(s):  
Red Blood Cells ◽  
Erythrocyte Sedimentation Rate ◽  
Linear Function ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Temperature Dependent ◽  
Experimental Conditions ◽  
Erythrocyte Sedimentation ◽  
Rouleau Formation ◽  
High Erythrocyte Sedimentation Rate

Rouleau formation was measured by an adaptation of an old method used by Ponder (1927, Q. J. Exp. Physiol. 16, 173–194). Blood was depleted of red cells to a low hematocrit. It was stirred under standardized conditions, and after various times, samples were examined by microscope. Mean rouleau length was taken as an index of rouleau formation. The method was used to observe changes of rouleau formation with time and temperature and with variations in erythrocyte sedimentation rate (E.S.R.). Under these experimental conditions, mean rouleau length increases with time up to 40 min. Over a range of hematocrit values between 0 and 4%, rouleau length is a linear function of hematocrit. At higher hematocrits random aggregations based on columnar units are seen. Rouleau formation is temperature dependent and maximum mean rouleau length occurs around 37 °C. Subjects with a high erythrocyte sedimentation rate tend to form long rouleaux. By exchanging red blood cells (R.B.C.) between two ABO Rh compatible patients with greatly differing E.S.R. and mean rouleau length, it was shown that the agent responsible was in the plasma and not on the R.B.C. The results were compared with a theory of aggregation which proved inadequate in some respects.

Influence of cadmium on hematological indicators of carp

2020 ◽  
Vol 1 ◽  
pp. 92-96
Author(s):  
L. Yu. Karpenko ◽  
◽  
P. A. Polistovskaya ◽  
A. I. Enukashvili ◽  
A. B. Balykina ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Red Blood Cells ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Power Plants ◽  
Cadmium Acetate ◽  
Control Group ◽  
Erythrocyte Sedimentation ◽  
Short Period

The article is devoted to the assessment of the toxic effects of cadmium acetate on fish. The research topic is relevant, since heavy metals, and especially cadmium, are ones of the most dangerous pollutants for the aquatic environment. After the emission of the already used water by the electricity-power plants into the reservoirs of circulating wa-ter, a sharp increase in the maximum permit-ted concentrations of heavy substances can be observed often, which act on hydrobi-onts for a rather short period of time. That is why, modeling this effect is necessary to study the effects of heavy metals. The aim of the assay was to study the hematological parameters of carp after exposure to various concentrations of cadmium acetate. During the experiment, 5 groups of fish were formed - 1 control group (10 fish), 4 experimental groups - 10 fish each. The experimental groups of fish were exposed in a solution of cadmium acetate (Cd (CH3COO) 2) with concentrations of 0.05 mg / l, 0.5 mg / l, 5 mg / l, 50 mg / l (exceeding the maximum permissible concentration of cadmium for fisheries in 10, 100, 1000 and 10000 times respectively). We have investigated: the erythrocyte sedimentation rate, the number of red blood cells in the blood and the con-centration of hemoglobin in the blood. Dur-ing the study, the authors noted an increase in the erythrocyte sedimentation rate due to the formation of conglomerates of blood cells with plasma proteins, as well as a de-crease in the number of red blood cells due to the toxic effect of cadmium acetate. A decrease in the concentration of hemoglobin in the blood was also observed, probably associated with a decrease in the number of red blood cells due to their lysis. With an increase in the concentration of the metal under study, there is a significant increase in ESR, a decrease in the level of hemoglobin and the number of red blood cells.

scholarly journals The Clinical Significance of Increased Serum Proinflammatory Cytokines, C-Reactive Protein, and Erythrocyte Sedimentation Rate in Patients with Hidradenitis Suppurativa

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
D. Jiménez-Gallo ◽  
R. de la Varga-Martínez ◽  
L. Ossorio-García ◽  
C. Albarrán-Planelles ◽  
C. Rodríguez ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Proinflammatory Cytokines ◽  
Sedimentation Rate ◽  
Hidradenitis Suppurativa ◽  
Staging System ◽  
Inflammatory Activity ◽  
Healthy Controls ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation

Objectives. To assess inflammatory serum markers including serum proinflammatory cytokines, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) according to the clinical inflammatory activity of patients with hidradenitis suppurativa (HS). Patients and Methods. Seventy-four patients with HS were studied based on the Hidradenitis Suppurativa-Physician Global Assessment (HS-PGA) score and Hurley staging system. Proinflammatory cytokines were measured using a multiplex cytokine assay. Twenty-two healthy volunteers were recruited. Results. Serum interleukin- (IL-) 6, IL-23, soluble tumour necrosis factor alpha (TNF-α) receptor I (sTNF-RI), CRP, and ESR were different in the patients with HS compared with those in the healthy controls (P<0.05). The levels of IL-1β, IL-6, IL-8, IL-10, IL-12p70, IL-17A, sTNF-RII, CRP, and ESR were significantly elevated according to inflammatory activity based on HS-PGA scores (r>0.25, P<0.05). The levels of IL-6 (r=0.53, P<0.001), CRP (r=0.54, P<0.001), and ESR (r=0.60, P<0.001) were especially well correlated with clinical inflammatory activity based on HS-PGA scores. The levels of IL-6, IL-8, sTNF-RI, sTNF-RII, CRP, and ESR were significantly elevated according to Hurley staging system. Conclusions. Serum proinflammatory cytokines, CRP, and ESR are increased in relation to the clinical inflammatory activity of patients with HS compared with healthy controls. Serum IL-6, CRP, and ESR are effective biomarkers for evaluating the severity of HS.

Elevated levels of anti-carbamylated protein antibody in patients with rheumatoid arthritis: association with disease activity and bone destruction

2020 ◽  
Vol 68 (6) ◽  
pp. 1186-1192
Author(s):  
Bei Zhang ◽  
Yimeng Lei ◽  
Xin Li ◽  
Ziyu Gao ◽  
Liping Xia ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Erythrocyte Sedimentation Rate ◽  
Antibody Level ◽  
Sedimentation Rate ◽  
Bone Erosion ◽  
Serum Levels ◽  
Double Negative ◽  
Healthy Controls ◽  
Erythrocyte Sedimentation

To measure the serum levels of anticarbamylated protein (CarP) antibodies in patients with rheumatoid arthritis (RA) in China and to evaluate the association of anti-CarP antibodies with clinical parameters and disease activity. 260 Chinese patients with RA, 40 patients with osteoarthritis (OA), 88 patients with spondyloarthritis (SpA) and 77 healthy controls were included. The serum levels of anti-CarP antibodies were detected by ELISA. Blood tests to detect the anticyclic citrullinated peptide (CCP) antibody level, rheumatoid factor (RF) level, erythrocyte sedimentation rate, C reactive protein level and Disease Activity Score in 28 joints using the erythrocyte sedimentation rate (DAS28-ESR) were performed by standard methods. Bone erosion was assessed by colour Doppler ultrasonography. A total of 18.8% of patients with RA and 9.4% of anti-CCP antibody and RF-double-negative patients were positive for anti-CarP antibody. The anti-CarP antibody level was significantly higher in patients with RA than in patients with OA or SpA and in healthy controls. Univariate and multivariate analyses showed that the level of anti-CarP antibody was positively correlated with DAS28-ESR; the higher a level of serum anti-CarP antibody, the higher the DAS28-ESR score. Anti-CarP-positive patients had higher disease activity scores than anti-CarP-negative patients. Moreover, anti-CarP-positive patients had a higher risk of developing bone erosion. The anti-CarP antibody was found to play an important role in the diagnosis of RA, especially in anti-CCP antibody and RF-double-negative patients. The anti-CarP antibody is a potential marker of disease activity and bone erosion in RA.

Society of Physicians at Kazan University

1927 ◽  
Vol 23 (1) ◽  
pp. 144-145
Keyword(s):  
Comparative Study ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Clinical Picture ◽  
Blood Cells ◽  
White Blood Cells ◽  
Not Given ◽  
Blood Picture ◽  
Erythrocyte Sedimentation

Session 9/Xll 1926. Prof. N. K. Goryaev: To estimate the content of white blood cells by smear (Schtzung). The report will be printed in "Kaz. Med. Jour.". Drs. N. Zakharov, N. Kudryashev and M. Aksyantsev: Experience in comparative study of immunobiological reactions in tbc clinic. Report, to be printed in Kaz. Med. Jour. Prof. P. N. Nikolaev pointed out about the report that it is impossible to perform all reactions studied by the reporters at the patient's bedside and that the doctor should never forget the personality of the patient in his work. Prof. N. K. Goryaev noted that a big drawback of the report was the lack of description of the clinical picture of the cases studied, as well as the fact that in parallel with the reactions studied the blood picture was not given. To study erythrocyte sedimentation rate Prof. Goryaev considers the Linzeamier's methodology inconvenient. In addition, Drs. Mastbaum and Aksyantsev commented on the reports.

Glucono-Delta-Lactone Mitigates the Erythrocyte Sedimentation Rate of Human Blood

2019 ◽  
Vol 6 (05) ◽  
pp. 4472-4473
Author(s):  
Kane Genser, MD ◽  
Charles R. Spillert PhD
Keyword(s):  
Molecular Weight ◽  
Erythrocyte Sedimentation Rate ◽  
Human Blood ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Low Molecular Weight ◽  
Erythrocyte Sedimentation

The clumping of circulating blood cells in many diseases gives rise to serious complications.  The sed-rate has been used to monitor blood for these changes. The protective low molecular weight agent used in this study was found to suppress the elevated sed-rate values.

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