EFFECT OF THE STERILIZATION METHOD ON THE MORPHOLOGY OF POLYELECTROLYTE FILMS

Two commercial laboratory mouse diets were sterilized by either high-vacuum autoclaving or gamma irradiation, and then fed to breeding mice of 2 different strains housed as monogamous pairs or trios. There was a statistically significant diet-by-sterilization-method interaction for total number of mice weaned, although neither the effect of 'diet' nor 'sterilization method' were statistically significant. Thus with diet FFG an average of 45.1 young was weaned per cage on irradiated diet compared with 55.6 on the autoclaved diet, while 51.4 mice were weaned per cage on irradiated Breeding Diet compared with 47.6 on the autoc1aved. This result suggests that the effects of sterilization by either method depend on the nature of the diet, so that it should be possible to design diets capable of being sterilized by autoclaving or irradiation. Palatability trials indicated a strong preference for irradiated diet over autoclaved diet.

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Alternative Sterilization Method for Heat Sensitive Products with Safe Residual Component

Journal of Medical Devices ◽

10.1115/1.4026784 ◽

2012 ◽

Vol 6 (1) ◽

Author(s):

Kristy Yates ◽

Jon Olson ◽

Alan Au ◽

Tom Gentle

Keyword(s):

Sterilization Method ◽

Residual Component

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Sterilisasi dan Induksi Daun Muda Durian (Durio zibethinus) Dalam Medium MS Dengan Penambahan Kinetin dan IAA Secara In Vitro

Planta Tropika Journal of Agro Science ◽

10.18196/pt.v1i1.3110 ◽

2021 ◽

Vol 1 (1) ◽

pp. 34-38

Author(s):

Gatot Supangkat ◽

Innaka Ageng Rineksane ◽

Kurniawati Pamuji

Keyword(s):

Vitamin C ◽

Callus Formation ◽

Tween 20 ◽

Second Phase ◽

Induction Phase ◽

Ms Medium ◽

Sterilization Method ◽

Central Java ◽

Durio Zibethinus

A research to study the sterilization method and application of Kinetin and IAA to induce the Durian young leaf (Durio zibethinus) in MS medium was conducted in Balai Benih Induk Hortikultura in Salaman Magelang district of Central Java started on September until December 2003. The Laboratory experiment was arranged in two phases, which were the optimation phase of sterilization and induction phase. At the first phase, the sterilization method used was the modification of Mulya (2001) method. The modification use of sterilant, vitamin C antioxidant, Alcohol 70 %, Benlate, Agrept, Tween-20 and Betadine were done to obtain effectiveness of the sterilization. Explants planted then in MS medium for two weeks. Contamination time, percentage of contamination and viabilitas (percentage of living explants) were observed then. At the second phase, the treatments were arranged in a 3 x 3 factorial completely randomized design (CRD) to observed the influence of Kinetin and IAA combination. The concentration of Kinetin observed were 2, 4, and 6 mg/I, where as the IAA concentration were 0.5, 1.0, and 1.5 mg/I. All treatments were repeated three times, with three samples on each replication. The percentage of browning explants, percentage of contaminated explants, site of contamination and percentage of explants live were observed at the end of incubation. The results showed that sterilization of Durian young leaves explants with 1 g/l deterjent for 15 minutes then by 2 g/l Benlate and Agrept for 10 minutes, then by 1 g/200 mg Vitamin C, then by Alcohol 70 % for 1 minute, then by 20% Clorox, then by 2 drip of Tween-20 for 10 minute and then by Betadine decreased the contamination down to 50 %, and this kind of sterilization was relatively better than the other kinds. Application of growth regulators were not able to induce explants growth, but stimulated callus formation at the cutting surface though, in the application of Kinetin 4 mg/1 + IAA 0,5 mg/I, Kinetin 4 mg/1 + IAA 1,5 mg/1, Kinetin 6 mg/I+ IAA 0,5 mg/1 and Kinetin 6 mg/l+IAA 1,0 mg/I.

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Effect of different sterilization methods on sensory quality and volatile flavor of flavor crab meat sauce

Quality Assurance and Safety of Crops & Foods ◽

10.15586/qas.v13i3.896 ◽

2021 ◽

Vol 13 (3) ◽

pp. 1-9

Author(s):

Yajun Zhu ◽

Haoran Wu ◽

Futian Wang ◽

Shaotong Jiang ◽

Lin Lin ◽

...

Keyword(s):

Sensory Quality ◽

Viable Count ◽

Sterilization Method ◽

Volatile Flavor ◽

Sensory Score ◽

Aldehydes And Ketones ◽

Microwave Sterilization ◽

New Type ◽

Total Ester ◽

Crab Meat

Flavor crab meat sauce (FCMS) is a new type of aquatic condiment that is not only rich in nutrition but also unique in terms of flavor. However, very little is known about how sterilization methods affect the sensory quality and volatile flavor of FCMS. In the present study, canned FCMS was subjected to the following sterilization methods: pasteurization (PS), microwave sterilization (MS), ultrasonic sterilization (US), and high temperature sterilization (HTS). Each sterilization method was evaluated on the basis of texture, color, sensory score, volatile flavor, and total viable counts in FCMS observed after sterilization. Results demonstrated that the total viable count decreased to 28, 26, 58, and 18 CFU/g respectively after PS, MS, US, and HTS treatments; no coliform group was detected in any of these samples. PS and MS had no significant effect on the texture of FCMS (P > 0.05), but US and HTS reduced hardness and viscosity of FCMS. MS and HTS significantly reduced the L*, a*, and b* values of FCMS (P < 0.05). The original brightness and red value of FCMS remained the same after PS and US. MS and HTS reduced the sensory score of FCMS to a great extent, while PS and US maintained the original sensory score of FCMS. Assessment of volatile flavor profile of FCMS post different sterilization treatments revealed that MS and HTS reduced the total ester content, while US reduced the total amount of alcohols in FCMS. However, PS not only maintained the original total amount of esters in FCMS, but also increased the content of alcohols, aldehydes, and ketones. Therefore, PS could be selected as a feasible sterilization method for FCMS among the four different sterilization methods.

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Cytotoxicity of three-dimensional paper-based models from a three-dimensional paper-based printer

NEMESIS ◽

10.14428/nemesis.v3i1.3883 ◽

2018 ◽

Vol 3 (1) ◽

pp. 15 ◽

Cited By ~ 1

Author(s):

Marcin Kozakiewicz ◽

Piotr Szymor ◽

Raphael Olszewski

Keyword(s):

Operating Room ◽

Dermal Fibroblast ◽

Statistical Significance ◽

Three Dimensional ◽

Dermal Fibroblasts ◽

Tissue Adhesive ◽

Biological Behaviour ◽

Sterilization Method ◽

Significant Difference ◽

Before And After

Objective: Our study aimed to determine the possibility of using models created with a low-cost, paper based 3D printer in an operating room. Therefore influence of different methods of sterilization on models was tested and cytotoxicity of generated models was determined. Material and methods: 30 cuboids divided into three groups were used for verification of shape stability after sterilization. Each group was sterilized either with: Ethylene oxide in temperature 55˚C, Hydrogen peroxide gas plasma in temperature 60˚C or Gamma irradiation at 21˚C, 25kGy. Each cuboid was measured using calliper three times before and three times after sterilization. Results were analysed statistically in Statgraphics Plus. Statistical significance was determined as p< 0.05. Sixty cylinders divided into six groups were used for cytotoxicity tests. Three of those groups were covered before sterilization with 2-octyl-cyanoacrylate. Each group was sterilized with one of the previously described methods. Cytotoxicity was tested by Nanostructural and Molecular Biophysics Laboratory in Technopark Lodz using normal adult human dermal fibroblasts. Survival of cells was tested using spectrophotometry with XTT and was defined as ratio of absorbency of tested probe to absorbency of control probe. Calcein/Ethidium dyeing test was performed according to LIVE/DEAD Viability/Cytotoxicity Kit protocol. Observation was done under Olympus GX71 fluorescence microscope. Results: There was no statistically significant difference for established statistical significance p=0.05 in cuboids dimensions before and after sterilization regardless of sterilization method. In XTT analysis all samples showed higher cytotoxicity against normal, human, adult dermal fibroblast culture when compared to positive control. ANOVA statistical analysis confirmed that 2-octyl cyanoacrylate coating of paper model improved biological behaviour of the material. It decreased cytotoxicity of the model independently of sterilization method. In calcein/ethidium dyeing test due to the high fluorescence of the background caused by cylinders of analysed substance it was impossible to perform the exact analysis of the number of marked cells. Conclusions: Acquired results allow to conclude that Mcor Technology Matrix 300 3D paper-based models can be used in operating room only if covered with cyanoacrylate tissue adhesive. Nemesis relevance: We found no statistically significant difference in cuboids dimensions before and after sterilization regardless of sterilization method. Three-dimensional paper-based models present with high cytotoxicity without coating.

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Effect of Sterilization Method and Other Modifications on the Wear Resistance of Acetabular Cups Made of Ultra-High Molecular Weight Polyethylene

The Journal of Bone and Joint Surgery (American) ◽

10.2106/00004623-200012000-00004 ◽

2000 ◽

Vol 82 (12) ◽

pp. 1708-1725 ◽

Cited By ~ 196

Author(s):

HARRY McKELLOP ◽

FU-WEN SHEN ◽

BIN LU ◽

PATRICIA CAMPBELL ◽

RONALD SALOVEY

Keyword(s):

Molecular Weight ◽

Wear Resistance ◽

High Molecular Weight ◽

Sterilization Method ◽

Ultra High Molecular Weight

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EFFECT OF STERILIZATION BY HEATING IN THE PRESENCE OF BACTERICIDE AND BACTERIAL FILTERED MEMBRANE ON THE STABILITY OF EYE DROPS CONTAINING 0.5% CHLORAMPHENICOL AT VARIOUS pH

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019v11i4.32976 ◽

2019 ◽

pp. 103-109

Author(s):

SRI AGUNG FITRI KUSUMA ◽

MARLINE ABDASSAH

Keyword(s):

Room Temperature ◽

Membrane Filter ◽

Storage Period ◽

Eye Drops ◽

Percentage Reduction ◽

Ph Change ◽

Sterilization Method ◽

Filter Membrane ◽

The Stability ◽

Antibacterial Potential

Objective: The purpose of this study was to determine a sterile 0.5% chloramphenicol eye drop formula with the best potency of antibacterial by determining the appropriate sterilization method and the supporting pH. Methods: 0.5% chloramphenicol was formulated with 0.01% thimerosal, which act as a bactericide and combines with borate buffer to produce eye drop formulas with variations in pH (6.8, 7.0 and 7.4). All formulas were stored at room temperature for 28 d and were evaluated, including: organoleptic of the preparations, sterility, pH stability, and the antibacterial potency of chloramphenicol in eye drops. Results: All dosage formulas did not undergo photodegradation reactions which were marked by no change in color until the end of the storage period. However, the formula with pH 6.8 which was sterilized by heating in a presence of bactericide, showed the presence of more particulate precipitates than in the pH 6.8 formula which was sterilized using membrane filter bacteria. However, both methods of sterilization produced sterile chloramphenicol eye drops. The preparation using a method of heat sterilization with bactericide decreased the pH greater than the preparation using a sterile bacterial filter sterilization method. C2 preparations at pH 7.0 and sterilized using the bacterial filter membrane sterilization method were more stable because they had the smallest pH change of 0.05 and the percentage reduction in antibacterial potential was smaller at 1.15%. Conclusion: The best treatment for the chloramphenicol eye drop was kept the pH formula at pH 7 and sterilized using bacterial filter membrane sterilization method.

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