NEW TECHNOLOGICAL APPROACH TO STUDY ROTATOR CUFF PATHOLOGY

2012 ◽  
Vol 15 (01) ◽  
pp. 1250010 ◽  
Author(s):  
Christopher Vercollone ◽  
Christopher Rashidifard ◽  
Shiyi Zan ◽  
Scott D. Martin ◽  
Mark E. Brezinski
Keyword(s):  
Rotator Cuff ◽  
Imaging Modality ◽  
Supraspinatus Tendon ◽  
Scar Tissue ◽  
Tissue Formation ◽  
Structural Abnormalities ◽  
Male Wistar Rats ◽  
Picrosirius Red ◽  
Cuff Repair ◽  
Scar Tissue Formation

Rotator cuff repair (RCR) is a crucial surgical procedure, but has unacceptable mechanical failure rates between 25–60%. Examining supplemental synergistic interventions, such as biological augmentations (ex: growth factors) to improve fibrocartilage formation rather than scar tissue formation, would make tears more amenable to surgical repair. Due to the large number of agents and application methods (and times), improved techniques are needed for assessing RCR in animals. In particular, high-resolution real-time imaging is needed to guide tissue engineering in animal models. Optical coherence tomography (OCT) is well suited for this role, with resolutions 25 × greater than any clinical imaging modality and an ability to identify organized collagen with polarization sensitive techniques. For example, it can determine severe collagen depletion in visually normal tendons. The images here show the first OCT and PS-OCT of the rotator cuff in male Wistar rats. The structure of the supraspinatus tendon, enthesis, and humerus are well defined. For histological comparison, this sample was stained with both Masson's Trichrome, to expose any structural abnormalities, and Picrosirius Red, to determine collagen content using a polarization filter. OCT studies offer the potential of understanding RCR failure mechanisms and potential tissue altering agents, substantially impacting outcomes.

scholarly journals Experimental Methods to Simulate and Evaluate Postsurgical Peripheral Nerve Scarring

2021 ◽  
Vol 10 (8) ◽  
pp. 1613
Author(s):  
Alessandro Crosio ◽  
Giulia Ronchi ◽  
Benedetta Elena Fornasari ◽  
Simonetta Odella ◽  
Stefania Raimondo ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Peripheral Nerves ◽  
Experimental Methods ◽  
Scar Tissue ◽  
Experimental Models ◽  
Tissue Formation ◽  
Surgical Interventions ◽  
Pubmed Database ◽  
Different Types ◽  
Scar Tissue Formation

As a consequence of trauma or surgical interventions on peripheral nerves, scar tissue can form, interfering with the capacity of the nerve to regenerate properly. Scar tissue may also lead to traction neuropathies, with functional dysfunction and pain for the patient. The search for effective antiadhesion products to prevent scar tissue formation has, therefore, become an important clinical challenge. In this review, we perform extensive research on the PubMed database, retrieving experimental papers on the prevention of peripheral nerve scarring. Different parameters have been considered and discussed, including the animal and nerve models used and the experimental methods employed to simulate and evaluate scar formation. An overview of the different types of antiadhesion devices and strategies investigated in experimental models is also provided. To successfully evaluate the efficacy of new antiscarring agents, it is necessary to have reliable animal models mimicking the complications of peripheral nerve scarring and also standard and quantitative parameters to evaluate perineural scars. So far, there are no standardized methods used in experimental research, and it is, therefore, difficult to compare the results of the different antiadhesion devices.

scholarly journals VEGF Receptor-2 Activation Mediated by VEGF-E Limits Scar Tissue Formation Following Cutaneous Injury

2018 ◽  
Vol 7 (8) ◽  
pp. 283-297 ◽  
Author(s):  
Lyn M. Wise ◽  
Gabriella S. Stuart ◽  
Nicola C. Real ◽  
Stephen B. Fleming ◽  
Andrew A. Mercer
Keyword(s):  
Scar Tissue ◽  
Vegf Receptor ◽  
Tissue Formation ◽  
Cutaneous Injury ◽  
Scar Tissue Formation

Freeze-dried chitosan-platelet-rich plasma implants improve supraspinatus tendon attachment in a transosseous rotator cuff repair model in the rabbit

2018 ◽  
Vol 33 (6) ◽  
pp. 792-807 ◽  
Author(s):  
Gabrielle Deprés-Tremblay ◽  
Anik Chevrier ◽  
Martyn Snow ◽  
Scott Rodeo ◽  
Michael D Buschmann
Keyword(s):  
Rotator Cuff ◽  
Rotator Cuff Repair ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Supraspinatus Tendon ◽  
Rotator Cuff Tears ◽  
Polymorphonuclear Cells ◽  
Post Surgery ◽  
Freeze Dried ◽  
Cuff Repair

Rotator cuff tears result in shoulder pain, stiffness, weakness and loss of motion. After surgical repair, high failure rates have been reported based on objective imaging and it is recognized that current surgical treatments need improvement. The aim of the study was to assess whether implants composed of freeze-dried chitosan (CS) solubilized in autologous platelet-rich plasma (PRP) can improve rotator cuff repair in a rabbit model. Complete tears were created bilaterally in the supraspinatus tendon of New Zealand White rabbits ( n = 4 in a pilot feasibility study followed by n = 13 in a larger efficacy study), which were repaired using transosseous suturing. On the treated side, CS-PRP implants were injected into the transosseous tunnels and the tendon itself, and healing was assessed histologically at time points ranging from one day to two months post-surgery. CS-PRP implants were resident within transosseous tunnels and adhered to tendon surfaces at one day post-surgery and induced recruitment of polymorphonuclear cells from 1 to 14 days. CS-PRP implants improved attachment of the supraspinatus tendon to the humeral head through increased bone remodelling at the greater tuberosity and also inhibited heterotopic ossification of the supraspinatus tendon at two months. In addition, the implants did not induce any detectable deleterious effects. This preliminary study provides the first evidence that CS-PRP implants could be effective in improving rotator cuff tendon attachment in a small animal model.

Atelocollagen Enhances the Healing of Rotator Cuff Tendon in Rabbit Model

2017 ◽  
Vol 45 (9) ◽  
pp. 2019-2027 ◽  
Author(s):  
Dong-Sam Suh ◽  
Jun-Keun Lee ◽  
Ji-Chul Yoo ◽  
Sang-Hun Woo ◽  
Ga-Ram Kim ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Rapid Development ◽  
Rabbit Model ◽  
Supraspinatus Tendon ◽  
Histological Evaluation ◽  
Control Group ◽  
Patch Type ◽  
Load To Failure ◽  
Cuff Repair ◽  
Experimental Group

Background: Failure of rotator cuff healing is a common complication despite the rapid development of surgical repair techniques for the torn rotator cuff. Purpose: To verify the effect of atelocollagen on tendon-to-bone healing in the rabbit supraspinatus tendon compared with conventional cuff repair. Study Design: Controlled laboratory study. Methods: A tear of the supraspinatus tendon was created and repaired in 46 New Zealand White rabbits. They were then randomly allocated into 2 groups (23 rabbits per group; 15 for histological and 8 for biomechanical test). In the experimental group, patch-type atelocollagen was implanted between bone and tendon during repair; in the control group, the torn tendon was repaired without atelocollagen. Each opposite shoulder served as a sham (tendon was exposed only). Histological evaluation was performed at 4, 8, and 12 weeks. Biomechanical tensile strength was tested 12 weeks after surgery. Results: Histological evaluation scores of the experimental group (4.0 ± 1.0) were significantly superior to those of the control group (7.7 ± 2.7) at 12 weeks ( P = .005). The load to failure was significantly higher in the experimental group (51.4 ± 3.9 N) than in the control group (36.4 ± 5.9 N) ( P = .001). Conclusion: Histological and biomechanical studies demonstrated better results in the experimental group using atelocollagen in a rabbit model of the supraspinatus tendon tear. Clinical Relevance: Atelocollagen patch could be used in the cuff repair site to enhance healing.

The effects of mitomycin C and 5-fluorouracil/triamcinolone on fibrosis/scar tissue formation secondary to subglottic trauma (experimental study)

2005 ◽  
Vol 26 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Hakan Cincik ◽  
Atila Gungor ◽  
Adem Cakmak ◽  
Atilla Omeroglu ◽  
Ethem Poyrazoglu ◽  
...  
Keyword(s):  
Experimental Study ◽  
Mitomycin C ◽  
Scar Tissue ◽  
Tissue Formation ◽  
Scar Tissue Formation

Identifying wound infections for veterinary nurses

2020 ◽  
Vol 11 (10) ◽  
pp. 447-451
Author(s):  
Amanda Curtis
Keyword(s):  
Healing Process ◽  
Scar Tissue ◽  
Care Practice ◽  
Wound Infections ◽  
Tissue Formation ◽  
Deep Tissue ◽  
The Difference ◽  
Bite Wounds ◽  
Major Factors ◽  
Scar Tissue Formation

Heavily contaminated wounds are a common occurrence in both referral and primary care practice, with traumatic and bite wounds being among the most typical aetiologies seen. Each type of wound can be affected by numerous factors that can inhibit the healing process, one of these major factors is infection. Wound infections and the formation of biofilms can present veterinary nurses with a variety of challenges, which is why it is important that we understand the difference between normal inflammatory signs and the signs of infection. The early identification of infection and biofilms within a wound can influence healing times, scar tissue formation and length of healing. This article aims to highlight the difference between inflammation and infection, the different levels of contamination within a wound, and ways to decipher between superficial and deep tissue infections.

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