Effects of bFGF on suppression of collagen type I accumulation and scar tissue formation during wound healing after mucoperiosteal denudation of rat palate

2008 ◽  
Vol 66 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Wookjin Choi ◽  
Hitoshi Kawanabe ◽  
Yoshihiko Sawa ◽  
Kunihisa Taniguchi ◽  
Hiroyuki Ishikawa
Keyword(s):  
Wound Healing ◽  
Collagen Type ◽  
Scar Tissue ◽  
Collagen Type I ◽  
Type I ◽  
Tissue Formation ◽  
Scar Tissue Formation

scholarly journals Therapeutic Effects of Acupuncture through Enhancement of Functional Angiogenesis and Granulogenesis in Rat Wound Healing

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sang In Park ◽  
Yun-Young Sunwoo ◽  
Yu Jin Jung ◽  
Woo Chul Chang ◽  
Moon-Seo Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Collagen Type ◽  
Interleukin 1 ◽  
Treated Group ◽  
Collagen Type I ◽  
Control Group ◽  
Therapeutic Effects ◽  
Type I ◽  
Matrix Remodeling ◽  
Cd 31

Acupuncture regulates inflammation process and growth factors by increasing blood circulation in affected areas. In this study, we examined whether acupuncture has an effect on wound healing in injured rat. Rats were assigned randomly into two groups: control group and acupuncture group. Acupuncture treatment was carried out at 8 sites around the wounded area. We analyzed the wound area, inflammatory cytokines, proliferation of resident cells, and angiogenesis and induction of extracelluar matrix remodeling. At 7 days after-wounding the wound size in acupuncture-treat group was decreased more significantly compared to control group. In addition, the protein levels of proinflammatory cytokines such as tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were significantly decreased compared to the control at 2 and 7 days post-wounding. Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31,α-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels,α-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. These results suggest that acupuncture may improve wound healing through decreasing pro-inflammatory response, increasing cell proliferation and angiogenesis, and inducing extracellular matrix remodeling.

Electrospun poly(vinyl) alcohol/collagen nanofibrous scaffold hybridized by graphene oxide for accelerated wound healing

2018 ◽  
Vol 41 (8) ◽  
pp. 467-473 ◽  
Author(s):  
Rethinam Senthil ◽  
Robert Berly ◽  
Thimmiah Bhargavi Ram ◽  
Nallathambi Gobi
Keyword(s):  
Wound Healing ◽  
Graphene Oxide ◽  
Vinyl Alcohol ◽  
Collagen Type ◽  
Collagen Type I ◽  
Fish Bone ◽  
Poly Vinyl Alcohol ◽  
Type I

Purpose: In this study, a blend of synthetic polymer (poly(vinyl) alcohol), natural polymer (collagen type I from fish bone), and graphene oxide nanoparticles is used to fabricate a composite nanofibrous scaffold, by electrospinning, for their potential application in accelerated wound healing. Methods: The scaffold was characterized for its physicochemical and mechanical properties. In vitro studies were carried out using human keratinocyte cell line (HaCaT) which proved the biocompatibility of the scaffold. In vivo study using mice model was carried out and the healing pattern was evaluated using histopathological studies. Results: Scaffold prepared from poly(vinyl) alcohol, collagen type I from fish bone, and graphene oxide possessed better physicochemical and mechanical properties. In addition, in vivo and in vitro studies showed its accelerated wound healing properties. Conclusion: The scaffold with required strength and biocompatibility may be tried as a wound dressing material in large animals after getting necessary approval.

scholarly journals Fibroblast migration and collagen deposition during dermal wound healing: mathematical modelling and clinical implications

2006 ◽  
Vol 364 (1843) ◽  
pp. 1385-1405 ◽  
Author(s):  
Steven McDougall ◽  
John Dallon ◽  
Jonathan Sherratt ◽  
Philip Maini
Keyword(s):  
Wound Healing ◽  
Diffusion Coefficient ◽  
Scar Tissue ◽  
Tissue Formation ◽  
Collagen Deposition ◽  
Dermal Wound Healing ◽  
Fibroblast Migration ◽  
Collagen Alignment ◽  
Dermal Wound ◽  
Scar Tissue Formation

The extent to which collagen alignment occurs during dermal wound healing determines the severity of scar tissue formation. We have modelled this using a multiscale approach, in which extracellular materials, for example collagen and fibrin, are modelled as continua, while fibroblasts are considered as discrete units. Within this model framework, we have explored the effects that different parameters have on the alignment process, and we have used the model to investigate how manipulation of transforming growth factor-β levels can reduce scar tissue formation. We briefly review this body of work, then extend the modelling framework to investigate the role played by leucocyte signalling in wound repair. To this end, fibroblast migration and collagen deposition within both the wound region and healthy peripheral tissue are considered. Trajectories of individual fibroblasts are determined as they migrate towards the wound region under the combined influence of collagen/fibrin alignment and gradients in a paracrine chemoattractant produced by leucocytes. The effects of a number of different physiological and cellular parameters upon the collagen alignment and repair integrity are assessed. These parameters include fibroblast concentration, cellular speed, fibroblast sensitivity to chemoattractant concentration and chemoattractant diffusion coefficient. Our results show that chemoattractant gradients lead to increased collagen alignment at the interface between the wound and the healthy tissue. Results show that there is a trade-off between wound integrity and the degree of scarring. The former is found to be optimized under conditions of a large chemoattractant diffusion coefficient, while the latter can be minimized when repair takes place in the presence of a competitive inhibitor to chemoattractants.

scholarly journals Elastomeric Cardiowrap Scaffolds Functionalized with Mesenchymal Stem Cells-Derived Exosomes Induce a Positive Modulation in the Inflammatory and Wound Healing Response of Mesenchymal Stem Cell and Macrophage

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 824
Author(s):  
Juan Carlos Chachques ◽  
Chiara Gardin ◽  
Nermine Lila ◽  
Letizia Ferroni ◽  
Veronique Migonney ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Collagen Type ◽  
Cell Function ◽  
Macrophage Polarization ◽  
Collagen Type I ◽  
Type I ◽  
Wound Healing Response ◽  
Biological Tool

A challenge in contractile restoration of myocardial scars is one of the principal aims in cardiovascular surgery. Recently, a new potent biological tool used within healing processes is represented by exosomes derived from mesenchymal stem cells (MSCs). These cells are the well-known extracellular nanovesicles released from cells to facilitate cell function and communication. In this work, a combination of elastomeric membranes and exosomes was obtained and tested as a bioimplant. Mesenchymal stem cells (MSCs) and macrophages were seeded into the scaffold (polycaprolactone) and filled with exosomes derived from MSCs. Cells were tested for proliferation with an MTT test, and for wound healing properties and macrophage polarization by gene expression. Moreover, morphological analyses of their ability to colonize the scaffolds surfaces have been further evaluated. Results confirm that exosomes were easily entrapped onto the surface of the elastomeric scaffolds, increasing the wound healing properties and collagen type I and vitronectin of the MSC, and improving the M2 phenotype of the macrophages, mainly thanks to the increase in miRNA124 and decrease in miRNA 125. We can conclude that the enrichment of elastomeric scaffolds functionalized with exosomes is as an effective strategy to improve myocardial regeneration.

scholarly journals Modulation of Extracellular Matrix by Scrophularia striata Extract in Vitro: A Potential Antiscarring Agent

2020 ◽  
Vol 15 (3) ◽  
Author(s):  
Pari Tamri ◽  
Rasool Haddadi ◽  
Fatemeh Javani Jouni
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Human Skin ◽  
Collagen Type ◽  
Collagen Type I ◽  
Collagen Content ◽  
Type I ◽  
Hypertrophic Scars ◽  
Total Collagen ◽  
Scrophularia Striata

Background: Hypertrophic scars are the consequences of the aberration of normal wound healing. To date, therapeutic strategies for abnormal scarring have been unsuccessful. ‎‏The abnormal extracellular‎ matrix is one of the most important contributing factors to ‎hypertrophic scars. ‏Scrophularia striata has been used in Iranian folk medicine for the treatment of burn wounds. ‏The plant extract accelerates wound healing and attenuates scar formation. Objectives: The study was performed to investigate the effects of Scrophularia striata hydroalcoholic extract (SSE) on MMP1, MMP8, fibronectin, collagen type I, and total collagen produced by human skin fibroblasts in the culture medium. Methods: The effects of SSE on the expression of MMP1, MMP8, fibronectin, and collagen type I in human skin fibroblast (HSF) were evaluated using Q-PCR and Western blotting methods. In addition, the effect of SSE on the total collagen content was measured in cultured HSF using Red Sirius Kit. Results: SSE significantly induced the expression of MMP1 and suppressed the production of fibronectin at the mRNA and protein levels. The total collagen content was significantly lower in SSE-treated cells than in untreated cells. SSE did not have any significant effect on MMP8 and collagen type I expression. Conclusions: The results of this study revealed that SSE could modulate the extracellular matrix turnover and had the potential for the prevention and treatment of hypertrophic scars.

scholarly journals Gene expression analysis in scars treated with silicone cream: a case series

2019 ◽  
Vol 5 ◽  
pp. 205951311986834 ◽  
Author(s):  
Robin Kikuchi ◽  
Abdullah J Khalil ◽  
Christopher I Zoumalan
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Matrix Protein ◽  
Collagen Type ◽  
Case Series ◽  
Human Growth ◽  
Scar Tissue ◽  
Type I ◽  
Genetic Expression ◽  
Type Iii

Background: In contrast to fetal scar tissue, adult scar tissue presents with visible scarring. Topical silicone creams have been shown to improve the appearance of scars. This case series compares the genetic expression of post-surgical scar tissues that received topical scar treatment with silicone cream, SKN2017B, or no treatment. SKN2017B is a recently formulated silicone-based scar cream that contains selective synthetic recombinant human growth factors, hyaluronic acid, and vitamin C. We hypothesise that scars treated with silicone-based scar creams have a more favourable genetic expression resembling a well-healing scar. Methods: Women who had undergone an abdominoplasty were included in this investigation and randomly assigned to treat part of the scar with topical silicone, another part with SKN2017B, and to leave a third part untreated. After four weeks, punch biopsies were taken and the RNA sequenced. Healthy abdominal skin was biopsied as baseline data. Genes of interest were identified and median values were calculated for the samples. Results: SKN2107B-treated scars demonstrated the lowest collagen type I to collagen type III ratio. Other key genes of interest in wound healing showed the lowest (favourable) expression of fibroblast activation protein alpha, lysyl oxidase and cartilage oligomeric matrix protein; the highest (favourable) expression of fibronectin type III domain containing 1 and matrix metallopeptidase 9 were found in scars treated with SKN2017B. Conclusion: The results of this small case series demonstrate a trend that those scars treated with topical silicone cream, notably SKN2017B, display the most favourable gene expression for wound healing.

scholarly journals A review on Vranagranthi (Keloid) in Ayurvedic and Modern Aspect

2019 ◽  
Vol 7 (06) ◽  
Author(s):  
Abhijeet Suresh Shinde
Keyword(s):  
Wound Healing ◽  
Scar Tissue ◽  
Review Article ◽  
Tissue Formation ◽  
Pathological Study ◽  
Modern Aspect ◽  
Surgical Scar ◽  
Severe Acne ◽  
Scar Tissue Formation ◽  
Regressive Phase

Hypertrophic appearing scar tissue formation is known as Keloids. The growth of keloid is as compare to other raised scars is more rapid and progressive. If the keloid is formed then it grow much larger than the wound that caused the scar. Keloid is hypertrophic appearing scar that continue to evolve over the time without quiescent or regressive phase in the process of wound healing [1,2]    The certain communities e.g. Africans having Keloid-prone skin. The cut, burn, pin prick or severe acne are the some examples that causes Keloid. A keloid can also form as chickenpox clear. Sometimes, a surgical scar becomes a keloid. In very rare cases, keloids form when people do not injure their skin. These are called “spontaneous keloids.” A keloid usually takes time to appear. After an injury, months can pass before this scar appears. A keloid can also form more quickly. Once it begins, a keloid can enlarge slowly for months or years. Ayurveda Vagbhatacharya described it is as Vrunagranthi. This review article is a comparative pathological study of Vrunagranthi with special references to keloid is found that having direct and much more identical Causes, features, pathology and prognostic between these.

The Temporal Course of Adenovirus-Mediated Gene Transfer in the Rat Larynx

2002 ◽  
Vol 126 (3) ◽  
pp. 281-290
Author(s):  
Jacobs Ian N. ◽  
Tufano Ralph P. ◽  
Walsh Danielle S. ◽  
Crombleholme Timothy
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Gene Transfer ◽  
Recombinant Adenovirus ◽  
Marker Gene ◽  
Care Facility ◽  
Scar Tissue ◽  
Complete Disappearance ◽  
Tissue Formation ◽  
Scar Tissue Formation

OBJECTIVE: Polypeptide growth factors have important influences on wound-healing and scar tissue formation. Specific growth factors or their inhibitors may potentially decrease scar tissue formation and prevent subglottic stenosis. Gene transfer using recombinant adenovirus may be an ideal method to mediate endogenous production of growth factors to inhibit fibrosis. STUDY DESIGN: The study incorporated adenovirus-mediated transduction of normal and stenotic rat larynges and histologic analysis of the sequential expression of a β-galactosidase marker gene over time. SETTING: The study was conducted at the animal care facility of an academic children's hospital. RESULTS: We report successful transduction in normal and injured rat larynx with peak expression of β-galactosidase at 2 days after transduction and almost complete disappearance by 7 days. There appeared to be an early inflammatory response to the viral injection, but at 7 and 14 days after injection (transduction) the uninjured rat larynges resumed a normal histologic appearance. All distant sites stained negative for β-galactosidase. CONCLUSION: Recombinant adenovirus-mediated gene transfer is feasible in the rat larynx with transient duration and limited toxicity. SIGNIFICANCE: Adenovirus-mediated gene transfer has the potential to deliver growth factors that modulate wound healing and inflammation in the larynx by inhibiting fibrosis.

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