IMPROVE OPTICAL CLEARING OF SKIN IN VITRO WITH PROPYLENE GLYCOL AS A PENETRATION ENHANCER

2009 ◽  
Vol 02 (03) ◽  
pp. 269-278 ◽  
Author(s):  
ZHONGWEI ZHI ◽  
ZHENZHEN HAN ◽  
QINGMING LUO ◽  
DAN ZHU
Keyword(s):  
Propylene Glycol ◽  
Diffuse Reflectance ◽  
Treatment Time ◽  
Optical Transmittance ◽  
Penetration Enhancer ◽  
Penetration Enhancers ◽  
Scattering Coefficient ◽  
Optical Clearing ◽  
Reduced Scattering Coefficient

In order to enhance the optical clearing effect of topically applied optical clearing agents (OCAs), we evaluated the effect of propylene glycol (PG) as a chemical penetration enhancer (PE) on optical clearing of skin in vitro by observation and measurement of optical-transmittance and diffuse-reflectance spectra. Three OCAs, i.e., glycerol, D-sorbitol and PEG400, and two other penetration enhancers, Azone and Thiazone, were used in this study. The results indicated that the decrease of reduced scattering coefficient caused by OCA/PG was larger than that by pure OCA, and the change by OCA/water was the least after the same treatment time. There were significant differences for the reduced scattering coefficient at 630 nm after 120 min application of agents between OCA and OCA/PG. The efficacy of optical clearing caused by OCA/PG depended on the OCA itself. When PEG400 was mixed with three different PEs, we found the optical clearing were different. The penetration enhancing ability of PG was much better compared to Azone, and suboptimal to Thiazone. Also, this study provides evidence for the use of PG as a PE in order to improve skin optical clearing.

PENGARUH PERBEDAAN CHEMICAL PENETRATION ENHANCER PADA PENETRASI TRANSDERMAL PATCH PROMETAZIN HCL

2017 ◽  
Vol 2 (2) ◽  
pp. 208
Author(s):  
Dwi Nurahmanto
Keyword(s):  
Drug Delivery ◽  
Oleic Acid ◽  
Propylene Glycol ◽  
Transdermal Drug Delivery ◽  
Isopropyl Alcohol ◽  
Oral Absorption ◽  
Penetration Enhancers ◽  
Weight Variation ◽  
Transdermal Drug Delivery Systems

This research aims is to create a promethazine HCl patch transdermal drug delivery systems with the most excellent penetration. Transdermal drug delivery can be efficiently used for the active agents which undergo rapid first pass metabolism and oral absorption is often disrupted by nausea and vomiting, hence the transdermal patches of promethazine HCl were prepared by using different penetration enhancers,  propylene glycol, oleic acid and isopropyl alcohol. The prepared formulations were evaluated for thickness, weight variation, moisture content, drug content, morphology, and in vitro permeation studies. The patch morphology studies were performed by Scanning Electron Microscopy (SEM). The amount of promethazine HCl transfered by propylene glycol  25.77 ± 3.0396 ug, isopropil alcohol 25.758 ± 2.9022 ug and oleic acid 25.017 ± 8.1300 ug. The penetration of promethazine HCl patch with oleic acid enhancer, produce the highest penetration than isopropil alcohol and propylene glycol. there was no difference in penetration using propylene glycol and isopropyl alcohol. Oleic acid is the best enhancer for preparations patch containing promethazine HCl although the amount of promethazine HCl contained is the least. The whole formulations comply with the  patch dosage requirements

scholarly journals Development of a Diffuse Reflectance Probe for In Situ Measurement of Inherent Optical Properties in Sea Ice

2021 ◽  
Author(s):  
Christophe Perron ◽  
Christian Katlein ◽  
Simon Lambert-Girard ◽  
Edouard Leymarie ◽  
Louis-Philippe Guinard ◽  
...  
Keyword(s):  
Monte Carlo ◽  
Optical Properties ◽  
Sea Ice ◽  
Phase Function ◽  
Diffuse Reflectance ◽  
Scattering Coefficient ◽  
Scattering Medium ◽  
Inherent Optical Properties ◽  
Reduced Scattering Coefficient

Abstract. Detailed characterization of the spatially and temporally varying inherent optical properties (IOPs) of sea ice is necessary to better predict energy and mass balances, as well as ice-associated primary production. Here we present the development of an active optical probe to measure IOPs of a small volume of sea ice (dm3) in situ and non-destructively. The probe is derived from the diffuse reflectance method used to measure the IOPs of human tissues. The instrument emits light into the ice by the use of optical fibre. Backscattered light is measured at multiple distances away from the source using several receiving fibres. Comparison to a Monte Carlo simulated lookup table allows to retrieve the absorption coefficient, the reduced scattering coefficient and a phase function similarity parameter γ, introduced by Bevilacqua and Depeursinge (1999), depending on the two first moments of the Legendre polynomials, allowing to analyze the backscattered light not satisfying the diffusion regime. Monte Carlo simulations showed that the depth cumulating 95% of the signal is between 40±2 mm and 270±20 mm depending on the source-detector distance and on the ice scattering properties. The magnitude of the instrument validation error on the reduced scattering coefficient ranged from 0.07% for the most scattering medium to 35% for the less scattering medium over the two orders of magnitude we validated. Vertical profiles of the reduced scattering coefficient were obtained with decimeter resolution on first-year Arctic interior sea ice on Baffin Island in early spring 2019. We measured values of up to 7.1 m−1 for the uppermost layer of interior ice and down to 0.15±0.05 m−1 for the bottommost layer. These values are in the range of polar interior sea ice measurements published by other authors. The inversion of the reduced scattering coefficient at this scale was strongly dependent of γ, highlighting the need to define the higher moments of the phase function. This novel developed probe provides a fast and reliable means for measurement of scattering into sea ice.

scholarly journals Evaluation of the Ability of a Novel Miconazole Formulation To Penetrate Nail by Using Three In Vitro Nail Models

2017 ◽  
Vol 61 (7) ◽  
Author(s):  
Luisa Christensen ◽  
Rob Turner ◽  
Sean Weaver ◽  
Francesco Caserta ◽  
Lisa Long ◽  
...  
Keyword(s):  
Penetration Enhancer ◽  
Penetration Enhancers ◽  
Zone Of Inhibition ◽  
Miconazole Nitrate ◽  
Bovine Hoof ◽  
Topical Medications ◽  
Selection Of

ABSTRACT In an effort to increase the efficacy of topical medications for treating onychomycosis, several new nail penetration enhancers were recently developed. In this study, the ability of 10% (wt/wt) miconazole nitrate combined with a penetration enhancer formulation to permeate the nail is demonstrated by the use of a selection of in vitro nail penetration assays. These assays included the bovine hoof, TurChub zone of inhibition, and infected-nail models.

scholarly journals Оптические свойства in vitro катарактальных хрусталиков глаза человека в видимом и ближнем ИК диапазонах-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (5) ◽  
pp. 656
Author(s):  
А.В. Беликов ◽  
А.М. Загорулько ◽  
С.Н. Смирнов ◽  
А.Н. Сергеев ◽  
А.А. Михайлова ◽  
...  
Keyword(s):  
Absorption Coefficient ◽  
Spectral Range ◽  
Anisotropy Factor ◽  
Optical Characteristics ◽  
Scattering Coefficient ◽  
Eye Lens ◽  
Human Eye ◽  
Scattering Anisotropy ◽  
Reduced Scattering Coefficient

Spectral dependences of absorption coefficient, scattering coefficient, anisotropy factor and reduced scattering coefficient for in vitro human eye lenses of different cataract type are investigated. The spectra of absorption coefficient and reduced scattering coefficient were obtained for the spectral range 400-2300 nm, and the spectra of scattering coefficient and scattering anisotropy factor - for the spectral range 400-1800 nm. The regions of wavelengths within which the spectra of the studied optical characteristics of the human eye lens are not statistically significantly different for samples with different stages of cataract maturity and, conversely, within which they are statistically significantly different, are determined.

scholarly journals Optical Clearing of Cranial Bone

2008 ◽  
Vol 2008 ◽  
pp. 1-8 ◽  
Author(s):  
Elina A. Genina ◽  
Alexey N. Bashkatov ◽  
Valery V. Tuchin
Keyword(s):  
Optical Properties ◽  
Propylene Glycol ◽  
Functional Imaging ◽  
Imaging Techniques ◽  
Cranial Bone ◽  
Light Penetration ◽  
Optical Clearing ◽  
Scattering Coefficients ◽  
Doubling Method

We present experimental results on optical properties of cranial bone controlled by administration of propylene glycol and glycerol. Both transmittance and reflectance spectra of human and porcine cranial bone in vitro were measured. For estimation of absorption and reduced scattering coefficients of the bone, the inverse adding-doubling method was used. The decrease of reflectance of the samples under action of the immersion agents was demonstrated. The experiments have shown that administration of the immersion liquids allows for effective controlling of tissue optical characteristics that makes bone more transparent, thereby increasing the ability of light penetration through the tissue. The presented results can be used in developing of functional imaging techniques, including OCT.

scholarly journals Development of a diffuse reflectance probe for in situ measurement of inherent optical properties in sea ice

2021 ◽  
Vol 15 (9) ◽  
pp. 4483-4500
Author(s):  
Christophe Perron ◽  
Christian Katlein ◽  
Simon Lambert-Girard ◽  
Edouard Leymarie ◽  
Louis-Philippe Guinard ◽  
...  
Keyword(s):  
Monte Carlo ◽  
Optical Properties ◽  
Absorption Coefficient ◽  
Sea Ice ◽  
Diffuse Reflectance ◽  
Scattering Coefficient ◽  
Scattering Medium ◽  
Inherent Optical Properties ◽  
Reduced Scattering Coefficient

Abstract. Detailed characterization of the spatially and temporally varying inherent optical properties (IOPs) of sea ice is necessary to better predict energy and mass balances, as well as ice-associated primary production. Here we present the development of an active optical probe to measure IOPs of a small volume of sea ice (dm3) in situ and non-destructively. The probe is derived from the diffuse reflectance method used to measure the IOPs of human tissues. The instrument emits light into the ice by the use of an optical fibre. Backscattered light is measured at multiple distances away from the source using several receiving fibres. Comparison to a Monte Carlo simulated lookup table allows, in theory, retrieval of the absorption coefficient, the reduced scattering coefficient and a phase function similarity parameter γ, introduced by Bevilacqua and Depeursinge (1999). γ depends on the two first moments of the Legendre polynomials, allowing the analysis of the backscattered light not satisfying the diffusion regime. The depth reached into the medium by detected photons was estimated using Monte Carlo simulations: the maximum depth reached by 95 % of the detected photons was between 40±2 and 270±20 mm depending on the source–detector distance and on the ice scattering properties. The magnitude of the instrument validation error on the reduced scattering coefficient ranged from 0.07 % for the most scattering medium to 35 % for the less scattering medium over the 2 orders of magnitude we validated. Fixing the absorption coefficient and γ, which proved difficult to measure, vertical profiles of the reduced scattering coefficient were obtained with decimetre resolution on first-year Arctic interior sea ice on Baffin Island in early spring 2019. We measured values of up to 7.1 m−1 for the uppermost layer of interior ice and down to 0.15±0.05 m−1 for the bottommost layer. These values are in the range of polar interior sea ice measurements published by other authors. The inversion of the reduced scattering coefficient at this scale was strongly dependent on the value of γ, highlighting the need to define the higher moments of the phase function. This newly developed probe provides a fast and reliable means for measurement of scattering in sea ice.

Perturbation of Platelet Adhesion to Endothelial Cells by Plasminogen Activation In Vitro

1997 ◽  
Vol 78 (02) ◽  
pp. 934-938 ◽  
Author(s):  
Hsiun-ing Chen ◽  
Yueh-I Wu ◽  
Yu-Lun Hsieh ◽  
Guey-Yueh Shi ◽  
Meei-Jyh Jiang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Platelet Adhesion ◽  
Treatment Time ◽  
Shape Change ◽  
Umbilical Vein ◽  
Tissue Type ◽  
Apical Surface ◽  
Plasminogen Activation

SummaryTo investigate whether the endothelium-platelet interactions may be altered by plasminogen activation, cultured human umbilical vein endothelial cells (ECs) were treated with tissue-type plasminogen activator (t-PA) in the presence of plasminogen, and platelet adhesion to ECs was subsequently measured by using a tapered flow chamber. Our results demonstrated that platelets adhered more readily to t-PA treated EC monolayer than to the control monolayer at all shear stress levels tested. This phenomenon was treatment time-dependent and dose-dependent, and it could be blocked by adding plasmin inhibitors, such as e-amino caproic acid and aprotinin. Adherent platelets on t-PA treated EC monolayer underwent more severe shape change than those on the control monolayer. While the extracellular matrix directly treated with t-PA attracted less platelets than the control matrix did, platelet adhesion to the matrix that was produced by t-PA-treated ECs was unaltered. These data suggest that t-PA treatment on ECs compromised antiplatelet-adhesion capability on their apical surface without altering the reactivity of their extracellular matrix towards platelets.

The effect of glycerin as penetration enhancer in a ketoprofen solid preparation–patch on in vitro penetration study through rat skin

2020 ◽  
Vol 23 (03) ◽  
pp. 71-83
Author(s):  
Pratama Ferina Nadya ◽  
Umam Choirul ◽  
Ameliana Lidya ◽  
Nurahmanto Dwi
Keyword(s):  
Penetration Enhancer ◽  
Rat Skin
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