scholarly journals Synthesis of a dithieno[3,2-b:2′,3′-d]silole-based conjugated polymer and characterization of its short wave near-infrared fluorescence properties

2020 ◽  
Vol 13 (05) ◽  
pp. 2041002 ◽  
Author(s):  
Chuantao Gu ◽  
Chunying Zheng ◽  
Bing Liu ◽  
Tingyu Feng ◽  
Jiping Ma ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Quantum Efficiency ◽  
Conjugated Polymer ◽  
Near Infrared ◽  
Fluorescence Emission ◽  
Short Wave ◽  
Fluorescent Imaging ◽  
Photothermal Effect ◽  
Side Chains ◽  
Donor Acceptor

Short wave near-infrared (SWIR, 900–1700[Formula: see text]nm) fluorescence imaging has attracted extensive research interest from scientists due to its high imaging quality. However, the variety of SWIR fluorescence imaging agents are quite limited and the corresponding quantum efficiency is relatively low. In this work, a novel conjugated polymer PDTSDTBT was reported, consisting of a donor unit with a tetrahedral Si (sp3) named DTS and an acceptor unit named DTBT with branched side chains. The design approach of endowing the donor–acceptor structure with the branched side chains successfully increase the fluorescence quantum efficiency. The polymer was prepared into nanoparticles by nanoprecipitation. The PDTSDTBT nanoparticles showed an absorption peak of 626[Formula: see text]nm and fluorescence emission peak of 924[Formula: see text]nm. The quantum efficiency of the nanoparticles is 0.53%, which is higher than that of nanotube fluorophores (0.4%). The nanoparticles also demonstrate a photothermal effect, the temperature of nanoparticles solution could reach [Formula: see text]C under excitation by 660[Formula: see text]nm laser. Therefore, the PDTSDTBT nanoparticles is an excellent fluorescent imaging agent with potential photothermal applications.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

scholarly journals Photothermal-assisted antibacterial application of GO-Ag against clinical isolated MDR E. coli

2019 ◽  
Author(s):  
Yuqing Chen ◽  
Wei Wu ◽  
Zeqiao Xu ◽  
Cheng Jiang ◽  
Shuang Han ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Near Infrared ◽  
Antibacterial Effect ◽  
Luria Bertani ◽  
Fluorescent Imaging ◽  
Photothermal Effect ◽  
Antibacterial Efficiency ◽  
E Coli ◽  
Morphology Characterization

Abstract Background: Treatment of multidrug-resistant (MDR) bacterial infection is a great challenge in public health. Herein, we provide a solution to this problem with the use of graphene oxide-silver (GO-Ag) nanocomposites as anti-bacterial agent. Methods: Following established protocols, silver nanoparticles were grown on graphene oxide sheets. Then, a series of in-vitro studies were conducted to validate the antibacterial efficiency of the GO-Ag nanocomposites against clinical MDR Escherichia coli (E. coli) strains. Firstly, minimum inhibitory concentrations (MICs) of different antimicrobials were tested against MDR E. Coli strains. Then, bacteria viability assessments were conducted with different nanomaterials in Luria-Bertani (LB) broth. Afterwards, photothermal irradiation was conducted on MDR E. coli with lower GO-Ag concentration. At last, fluorescent imaging and morphology characterization using scanning electron microscope (SEM) were done to find the possible cause of antibacterial effect. Results: GO-Ag nanocomposites showed the highest antibacterial efficiency among tested antimicrobials. Synergetic antibacterial effect was observed in GO-Ag nanocomposites treated group. The remained bacteria viabilities were 4.4% and 4.1% respectively for different bacteria strains with GO-Ag concentration at 14.0 µg mL-1. In addition, GO-Ag nanocomposites have strong absorption in the near-infrared field and can convert the electromagnetic energy to heat. With the use of this photothermal effect, effective sterilization could be achieved using GO-Ag nanocomposites concentration as low as 7.0 µg mL-1. Fluorescent imaging and morphology characterization were used to analyze bacteria living status, which uncovered that bacteria integrity was disrupted after GO-Ag nanocomposites treatment. Conclusions: GO-Ag nanocomposites are proved to be efficient antibacterial agent against multi-drug resistant E. coli. Their strong antibacterial effect arises from inherent antibacterial property and photothermal effect that provides aid for bacteria killing.

scholarly journals Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

2020 ◽  
Vol 8 (1) ◽  
pp. 53
Author(s):  
Thomas Oh ◽  
Jittiwat Sermsripong ◽  
Barry W. Hicks
Keyword(s):  
Fluorescence Imaging ◽  
Large Scale ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Florida Keys ◽  
Nir Fluorescence ◽  
Novel Method ◽  
Near Infrared Fluorescence Imaging

Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments. Here, we report a novel method of selectively imaging chlorophyll distributions in coral in situ using only the near infrared (NIR) fluorescence emission from chlorophyll. Commercially available equipment was assembled that allowed the sequential imaging of visible, visible-fluorescent, and NIR-fluorescent pigments on the same corals. The relative distributions of chlorophyll and fluorescent proteins (GFPs) were examined in numerous corals in the Caribbean Sea, the Egyptian Red Sea, the Indonesian Dampier Strait, and the Florida Keys. Below 2 m depth, solar induced NIR chlorophyll fluorescence can be imaged in daylight without external lighting, thus, it is much easier to do than visible fluorescence imaging done at night. The distributions of chlorophyll and GFPs are unique in every species examined, and while there are some tissues where both fluorophores are co-resident, often tissues are selectively enriched in only one of these fluorescent pigments. Although laboratory studies have clearly shown that GFPs can be photo-protective, their inability to prevent large scale bleaching events in situ may be due to their limited tissue distribution.

scholarly journals Fluorescence Imaging: Bright Far-Red/Near-Infrared Conjugated Polymer Nanoparticles for In Vivo Bioimaging (Small 18/2013)

Small ◽  
2013 ◽  
Vol 9 (18) ◽  
pp. 3092-3092 ◽  
Author(s):  
Dan Ding ◽  
Jie Liu ◽  
Guangxue Feng ◽  
Kai Li ◽  
Yong Hu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Conjugated Polymer ◽  
Near Infrared ◽  
Polymer Nanoparticles ◽  
Conjugated Polymer Nanoparticles

A Donor–Acceptor Conjugated Polymer with Alternating Isoindigo Derivative and Bithiophene Units for Near-Infrared Modulated Cancer Thermo-Chemotherapy

2016 ◽  
Vol 8 (30) ◽  
pp. 19312-19320 ◽  
Author(s):  
Dong-Dong Li ◽  
Jun-Xia Wang ◽  
Yan Ma ◽  
Hai-Sheng Qian ◽  
Dong Wang ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Near Infrared ◽  
Donor Acceptor

scholarly journals Near-infrared fluorescent imaging techniques for the detection and preservation of parathyroid glands during endocrine surgery

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marco Stefano Demarchi ◽  
Wolfram Karenovics ◽  
Benoît Bédat ◽  
Frédéric Triponez
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging Techniques ◽  
Fluorescent Imaging ◽  
Parathyroid Glands ◽  
Endocrine Surgery ◽  
Icg Angiography ◽  
Real Time Identification

Abstract Objectives In over 30% of all thyroid surgeries, complications arise from transient and definitive hypoparathyroidism, underscoring the need for real-time identification and preservation of parathyroid glands (PGs). Here, we evaluate the promising intraoperative optical technologies available for the identification, preservation, and functional assessment of PGs to enhance endocrine surgery. Methods We performed a review of the literature to identify published studies on fluorescence imaging in thyroid and parathyroid surgery. Results Fluorescence imaging is a well-demonstrated approach for both in vivo and in vitro localization of specific cells or tissues, and is gaining popularity as a technique to detect PGs during endocrine surgery. Autofluorescence (AF) imaging and indocyanine green (ICG) angiography are two emerging optical techniques to improve outcomes in thyroid and parathyroid surgeries. Near-infrared-guided technology has significantly contributed to the localization of PGs, through the detection of glandular AF. Perfusion through the PGs can be visualized with ICG, which can also reveal the blood supply after dissection. Conclusions Near infrared AF and ICG angiography, providing a valuable spatial and anatomical information, can decrease the incidence of complications in thyroid surgery.

A phase II/III randomized, blinded study of tozuleristide for fluorescence imaging detection during neurosurgical resection of pediatric primary central nervous system (CNS) tumors: PNOC012 (Pacific Pediatric Neuro-oncology Consortium).

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. TPS2575-TPS2575
Author(s):  
Sarah Leary ◽  
Jason E. Blatt ◽  
Alan R. Cohen ◽  
Kenneth J. Cohen ◽  
Bonnie Cole ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Tumor Tissue ◽  
Phase 1 ◽  
Tumor Resection ◽  
Fluorescent Imaging ◽  
Low Grade ◽  
Primary Efficacy ◽  
Imaging Device ◽  
Patient Reported

TPS2575 Background: Tozuleristide (also known as BLZ-100 or Tumor Paint) is a fluorescent imaging drug designed to specifically label and accumulate in tumor tissue, thus enabling more precise surgical tumor resection intraoperatively. Tozuleristide achieves tumor targeting through the peptide portion of the molecule, a modified chlorotoxin peptide, and its imaging properties from a coupled near-infrared fluorescent dye, an indocyanine green. Tozuleristide has been studied in 4 Phase 1 studies, including a trial in pediatric brain cancer subjects. No tozuleristide SAEs or dose limiting toxicity were observed in the 97 subjects treated in the Phase 1 program at doses up to 30 mg in adults or 17.3 mg/m2 in pediatrics (Hansen S et al, WMIC 2018, P196). Eighty percent of pediatric subjects receiving tozuleristide had tumors considered fluorescence positive, including high and low grade glioma, ependymoma, and medulloblastoma. Methods: This study randomizes subjects in a 1:10 ratio to standard of care or tozuleristide arms. The primary efficacy objectives and endpoints are based on equivocal regions of tissue encountered in surgery. Prior to fluorescence assessment, the surgeon assesses the suspected nature of the tissue (more likely tumor/less likely tumor). Tissue specimens of equivocal regions are collected for blinded central pathology assessment. Sensitivity and specificity of the surgeon’s designation, fluorescence assessment, and ratios of surgeon to fluorescence assessments comprise the primary efficacy analyses. Tozuleristide is given as an IV bolus dose of 15 mg/m2 to pediatric subjects 1 to 36 hours prior to surgery. Subjects must have a MRI documented lesion consistent with a CNS tumor for which resection is planned. Measures of safety include adverse events, laboratory measures of hematology, liver and kidney function and changes in vital signs and ECGs. Pharmacokinetic blood samples are collected up to 3 hr post dose. Fluorescence imaging is assessed during surgery using an investigational “Canvas System” imaging device attached to a surgical microscope. Collected pathology specimens will also be subjected to further genetic, molecular and pathology studies, including fluorescence assessment of frozen tissue sections. SAEs and patient reported outcomes are collected for 3 months. The SMC for the study last reviewed the data for this study in July 2019 and recommended the trial continue as planned. Clinical trial information: NCT03579602 .

Low-Bandgap Conjugated Polymer Dots for Near-Infrared Fluorescence Imaging

2018 ◽  
Vol 1 (9) ◽  
pp. 4801-4808 ◽  
Author(s):  
Charu V. Rohatgi ◽  
Takaaki Harada ◽  
Eleanor F. Need ◽  
Marta Krasowska ◽  
David A. Beattie ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Conjugated Polymer ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Low Bandgap ◽  
Polymer Dots ◽  
Near Infrared Fluorescence Imaging

High-stability NIR-II Fluorescence Polymer Synthesized by Atom Transfer Radical Polymerization for High-Resolution NIR-II Imaging Application

2021 ◽  
Author(s):  
Kun He ◽  
Shangyu Chen ◽  
Wen-Juan Xu ◽  
Xiaoyan Tai ◽  
Yan Chen ◽  
...  
Keyword(s):  
High Resolution ◽  
Atom Transfer Radical Polymerization ◽  
Small Molecules ◽  
Radical Polymerization ◽  
High Stability ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Optical Images ◽  
Imaging Application ◽  
Donor Acceptor

Near-infrared II (NIR-II, 1000 - 1700 nm) fluorescent imaging (FI) has been reported to achieve optical images with higher resolution and deeper penetration. Among organic NIR-II small molecules, donor-acceptor-donor (D-A-D)...

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