High-stability NIR-II Fluorescence Polymer Synthesized by Atom Transfer Radical Polymerization for High-Resolution NIR-II Imaging Application

2021 ◽  
Author(s):  
Kun He ◽  
Shangyu Chen ◽  
Wen-Juan Xu ◽  
Xiaoyan Tai ◽  
Yan Chen ◽  
...  
Keyword(s):  
High Resolution ◽  
Atom Transfer Radical Polymerization ◽  
Small Molecules ◽  
Radical Polymerization ◽  
High Stability ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Optical Images ◽  
Imaging Application ◽  
Donor Acceptor

Near-infrared II (NIR-II, 1000 - 1700 nm) fluorescent imaging (FI) has been reported to achieve optical images with higher resolution and deeper penetration. Among organic NIR-II small molecules, donor-acceptor-donor (D-A-D)...

Preparation of an iron porphyrin-based polymer monolithic column via atom transfer radical polymerization for the separation of proteins and small molecules

2017 ◽  
Vol 9 (17) ◽  
pp. 2596-2602 ◽  
Author(s):  
Zhen Wei ◽  
Doudou Zhang ◽  
Cheng Li ◽  
Ligai Bai ◽  
Haiyan Liu ◽  
...  
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Small Molecules ◽  
Radical Polymerization ◽  
Monolithic Column ◽  
Iron Porphyrin ◽  
Atom Transfer ◽  
Polymer Monolithic Column ◽  
Separation Of Proteins

An iron porphyrin-based polymer monolithic column was prepared via ATRP for the separation of proteins from bio-matrices and small molecules.

Atom Transfer Radical Polymerization Driven by Near-Infrared Light with Recyclable Upconversion Nanoparticles

2020 ◽  
Vol 53 (12) ◽  
pp. 4678-4684 ◽  
Author(s):  
Wenjie Zhang ◽  
Jianhao He ◽  
Chunna Lv ◽  
Qianyi Wang ◽  
Xinchang Pang ◽  
...  
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Near Infrared ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Atom Transfer ◽  
Near Infrared Light

scholarly journals Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Simona-Adriana Manea ◽  
Mihaela-Loredana Vlad ◽  
Daniela Rebleanu ◽  
Alexandra-Gela Lazar ◽  
Ioana Madalina Fenyo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
High Resolution ◽  
Near Infrared ◽  
Reactive Oxygen ◽  
Experimental Models ◽  
Fluorescent Imaging ◽  
Quantitative Detection ◽  
Oxygen Species ◽  
Near Infrared Fluorescence ◽  
Models Of Disease

Excessive production of reactive oxygen species (ROS) and the ensuing oxidative stress are instrumental in all phases of atherosclerosis. Despite the major achievements in understanding the regulatory pathways and molecular sources of ROS in the vasculature, the specific detection and quantification of ROS in experimental models of disease remain a challenge. We aimed to develop a reliable and straightforward imaging procedure to interrogate the ROS overproduction in the vasculature and in various organs/tissues in atherosclerosis. To this purpose, the cell-impermeant ROS Brite™ 700 (RB700) probe that produces bright near-infrared fluorescence upon ROS oxidation was encapsulated into VCAM-1-targeted, sterically stabilized liposomes (VLp). Cultured human endothelial cells (EC) and macrophages (Mac) were used for in vitro experiments. C57BL6/J and ApoE-/- mice were randomized to receive normal or high-fat, cholesterol-rich diet for 10 or 32 weeks. The mice received a retroorbital injection with fluorescent tagged VLp incorporating RB700 (VLp-RB700). After two hours, the specific signals of the oxidized RB700 and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-DSPE), inserted into liposome bilayers, were measured ex vivo in the mouse aorta and various organs by high-resolution fluorescent imaging. VLp-RB700 was efficiently taken up by cultured human EC and Mac, as confirmed by fluorescence microscopy and spectrofluorimetry. After systemic administration in atherosclerotic ApoE-/- mice, VLp-RB700 were efficiently concentrated at the sites of aortic lesions, as indicated by the augmented NBD fluorescence. Significant increases in oxidized RB700 signal were detected in the aorta and in the liver and kidney of atherosclerotic ApoE-/- mice. RB700 encapsulation into sterically stabilized VCAM-1-sensitive Lp could be a novel strategy for the qualitative and quantitative detection of ROS in the vasculature and various organs and tissues in animal models of disease. The accurate and precise detection of ROS in experimental models of disease could ease the translation of the results to human pathologies.

scholarly journals Donor–acceptor–acceptor (D–A–A) type 1,8-naphthalimides as non-fullerene small molecule acceptors for bulk heterojunction solar cells

2017 ◽  
Vol 8 (3) ◽  
pp. 2017-2024 ◽  
Author(s):  
Prabhat Gautam ◽  
Rahul Sharma ◽  
Rajneesh Misra ◽  
M. L. Keshtov ◽  
S. A. Kuklin ◽  
...  
Keyword(s):  
Solar Cells ◽  
Small Molecules ◽  
Small Molecule ◽  
Near Infrared ◽  
Bulk Heterojunction ◽  
Strong Absorption ◽  
Heterojunction Solar Cells ◽  
Bulk Heterojunction Solar Cells ◽  
Donor Acceptor

Donor–acceptor–acceptor (D–A–A) type 1,8-naphthalimide based small molecules SM1 and SM2 functionalized with tetracyanobutadiene (TCBD) and dicyanoquino-dimethane (DCNQ) modules, showing strong absorption in the visible and near-infrared (NIR) region are reported.

scholarly journals Synthesis of a dithieno[3,2-b:2′,3′-d]silole-based conjugated polymer and characterization of its short wave near-infrared fluorescence properties

2020 ◽  
Vol 13 (05) ◽  
pp. 2041002 ◽  
Author(s):  
Chuantao Gu ◽  
Chunying Zheng ◽  
Bing Liu ◽  
Tingyu Feng ◽  
Jiping Ma ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Quantum Efficiency ◽  
Conjugated Polymer ◽  
Near Infrared ◽  
Fluorescence Emission ◽  
Short Wave ◽  
Fluorescent Imaging ◽  
Photothermal Effect ◽  
Side Chains ◽  
Donor Acceptor

Short wave near-infrared (SWIR, 900–1700[Formula: see text]nm) fluorescence imaging has attracted extensive research interest from scientists due to its high imaging quality. However, the variety of SWIR fluorescence imaging agents are quite limited and the corresponding quantum efficiency is relatively low. In this work, a novel conjugated polymer PDTSDTBT was reported, consisting of a donor unit with a tetrahedral Si (sp3) named DTS and an acceptor unit named DTBT with branched side chains. The design approach of endowing the donor–acceptor structure with the branched side chains successfully increase the fluorescence quantum efficiency. The polymer was prepared into nanoparticles by nanoprecipitation. The PDTSDTBT nanoparticles showed an absorption peak of 626[Formula: see text]nm and fluorescence emission peak of 924[Formula: see text]nm. The quantum efficiency of the nanoparticles is 0.53%, which is higher than that of nanotube fluorophores (0.4%). The nanoparticles also demonstrate a photothermal effect, the temperature of nanoparticles solution could reach [Formula: see text]C under excitation by 660[Formula: see text]nm laser. Therefore, the PDTSDTBT nanoparticles is an excellent fluorescent imaging agent with potential photothermal applications.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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