In-Line Monitoring of Bacteria in Drinking Water By Infrared Spectroscopy and Micro-Flow Cytometry

2014 ◽  
Keyword(s):  
Flow Cytometry ◽  
Drinking Water ◽  
Infrared Spectroscopy ◽  
Micro Flow

Detection of microbial disturbances in a drinking water microbial community through continuous acquisition and advanced analysis of flow cytometry data

2018 ◽  
Vol 145 ◽  
pp. 73-82 ◽  
Author(s):  
Ruben Props ◽  
Peter Rubbens ◽  
Michael Besmer ◽  
Benjamin Buysschaert ◽  
Jurg Sigrist ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Drinking Water ◽  
Microbial Community ◽  
Flow Cytometry Data ◽  
Advanced Analysis

scholarly journals Online flow cytometry reveals microbial dynamics influenced by concurrent natural and operational events in groundwater used for drinking water treatment

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Michael D. Besmer ◽  
Jannis Epting ◽  
Rebecca M. Page ◽  
Jürg A. Sigrist ◽  
Peter Huggenberger ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Drinking Water ◽  
Water Treatment ◽  
Drinking Water Treatment ◽  
Microbial Dynamics

scholarly journals Self-Mixing Interferometry-Based Micro Flow Cytometry System for Label-Free Cells Classification

2020 ◽  
Vol 10 (2) ◽  
pp. 478 ◽  
Author(s):  
Yu Zhao ◽  
Xuefei Shen ◽  
Menglei Zhang ◽  
Jingwen Yu ◽  
Jintao Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Speed ◽  
Label Free ◽  
Cell Type ◽  
Fluorescence Staining ◽  
Label Free Detection ◽  
Measurement Results ◽  
Micro Flow ◽  
The Individual ◽  
Type Classification

In this paper, we present a novel optical microfluidic cytometry scheme for label-free detection of cells that is based on the self-mixing interferometry (SMI) technique. This device enables simple, fast and accurate detection of the individual cell characteristics and efficient cell type classification. We also propose a novel parameter to classify the cell or particle size. Artificial polystyrene beads and human living cells were measured using this system, and the SMI signal properties were statistically evaluated. The capability of the proposed cytometer for cell type discrimination and size classification has been validated by the measurement results. Our study can provide a very simple technique for cell enumeration and classification without any extra devices such as high-speed camera, photomultiplier and spectrometer. Moreover, the fluorescence staining operation which is necessary in traditional flow cytometry methods is not required either in our system.

Micro Flow Cytometry

2012 ◽  
pp. 1375-1375
Author(s):  
Gabriela Juarez-Martinez ◽  
Alessandro Chiolerio ◽  
Paolo Allia ◽  
Martino Poggio ◽  
Christian L. Degen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Micro Flow

A sheath-less electric impedance micro-flow cytometry device for rapid label-free cell classification and viability testing

2017 ◽  
Vol 9 (7) ◽  
pp. 1201-1212 ◽  
Author(s):  
Xinwu Xie ◽  
Zhen Cheng ◽  
Youchun Xu ◽  
Ran Liu ◽  
Qi Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Simple Structure ◽  
Electrical Conductance ◽  
Free Cell ◽  
Cell Detection ◽  
Label Free ◽  
Electric Impedance ◽  
Single Cell Detection ◽  
Micro Flow

A sheath-less PDMS microfluidic IFC device with a simple structure was constructed, with a good performance in single-cell detection. The electrical conductance and susceptance were used to differentiate the beads/cells.

scholarly journals Microplastics Detection in Streaming Tap Water with Raman Spectroscopy

Sensors ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1839 ◽  
Author(s):  
Ann-Kathrin Kniggendorf ◽  
Christoph Wetzel ◽  
Bernhard Roth
Keyword(s):  
Raman Spectroscopy ◽  
Drinking Water ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Surface Waters ◽  
Tap Water ◽  
Flow Cell ◽  
Proof Of Concept ◽  
Point Of Entry ◽  
Custom Made

Microplastic particles have been found in drinking water sources worldwide and, thus, also in our food and beverages. Especially small microplastics, with sizes of 1 mm and less, cannot be identified reliably without spectroscopic means such as Fourier transform infrared spectroscopy (FTIR) or Raman spectroscopy, usually applied to the particles extracted from the samples. However, for drinking and tap water, with its comparatively low biological loads, direct observation may be possible and allows a point-of-entry monitoring for beverages and food to ensure uncontaminated drinking water is being used. In a proof of concept, we apply Raman spectroscopy to observe individual microplastic particles in tap water with added particulate and fluorescent contaminants streaming with 1 L/h through a custom-made flow cell. We evaluated several tubing materials for compatibility with microplastic suspensions containing three different polymers widely found in microplastic surveys worldwide. The experiment promises the monitoring of streaming tap water and even clear surface waters for microplastics smaller than 0.1 mm.

Monitoring of three drinking water treatment plants using flow cytometry

2014 ◽  
Vol 14 (5) ◽  
pp. 850-856 ◽  
Author(s):  
K. Helmi ◽  
A. Watt ◽  
P. Jacob ◽  
I. Ben-Hadj-Salah ◽  
A. Henry ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Drinking Water ◽  
Water Treatment ◽  
Cell Activity ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Culturable Bacteria ◽  
Culture Methods ◽  
Water Treatment Plants ◽  
Sampling Campaign

A 4-month sampling campaign has been conducted for the monitoring of three drinking water treatment plants using flow cytometry and culture-based methods to provide information related to changes in bacterial concentration according to treatments. Flow cytometry is a fast and user-friendly technique enabling bacteria quantification and viability assessment in less than 1 hour. Specific profiles regarding log-reduction of total bacteria were obtained for each treatment plant. Chlorination appeared to be the most effective by causing metabolism inactivation and nucleic acid damages. Ozonation showed a significant impact on cell activity in contrast with ultraviolet treatment which strongly affected bacterial DNA. In addition, the results showed that active bacteria quantified by flow cytometry were significantly correlated with culturable bacteria. This alternative approach appeared as gainful compared to culture methods as it greatly facilitates the diagnosis of treatment plant process for drinking water production monitoring.

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