Cortical bone stem cells modify cardiac inflammation after myocardial infarction by inducing a novel macrophage phenotype

Cortical bone stem cell (CBSC) therapy after myocardial infarction alters the inflammatory response to cardiac injury. We found that cortical bone stem cell therapy induces a unique macrophage phenotype in vitro and can modulate macrophage/fibroblast cross talk.

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Abstract 707: Cortical-bone Stem Cell Therapy Alters the Inflammatory Response After Myocardial Infarction

Circulation Research ◽

10.1161/res.125.suppl_1.707 ◽

2019 ◽

Vol 125 (Suppl_1) ◽

Author(s):

Alexander Hobby ◽

Remus Berretta ◽

Giulia Borghetti ◽

Eric Feldsott ◽

Deborah Eaton ◽

...

Keyword(s):

Myocardial Infarction ◽

Stem Cell ◽

Inflammatory Response ◽

Cell Therapy ◽

Cortical Bone ◽

Stem Cell Therapy

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Positively Correlated CD47 Activation and Autophagy in Umbilical Cord Blood-Derived Mesenchymal Stem Cells during Senescence

Stem Cells International ◽

10.1155/2021/5582792 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Gee-Hye Kim ◽

Yun Kyung Bae ◽

Ji Hye Kwon ◽

Miyeon Kim ◽

Soo Jin Choi ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cell Growth ◽

Cell Therapy ◽

Critical Role ◽

Therapeutic Effects ◽

Stem Cell Maintenance ◽

Selection Of

Autophagy plays a critical role in stem cell maintenance and is related to cell growth and cellular senescence. It is important to find a quality-control marker for predicting senescent cells. This study verified that CD47 could be a candidate to select efficient mesenchymal stem cells (MSCs) to enhance the therapeutic effects of stem cell therapy by analyzing the antibody surface array. CD47 expression was significantly decreased during the expansion of MSCs in vitro ( p < 0.01 ), with decreased CD47 expression correlated with accelerated senescence phenotype, which affected cell growth. UCB-MSCs transfected with CD47 siRNA significantly triggered the downregulation of pRB and upregulation of pp38, which are senescence-related markers. Additionally, autophagy-related markers, ATG5, ATG12, Beclin1, and LC3B, revealed significant downregulation with CD47 siRNA transfection. Furthermore, autophagy flux following treatment with an autophagy inducer, rapamycin, has shown that CD47 is a key player in autophagy and senescence to maintain and regulate the growth of MSCs, suggesting that CD47 may be a critical key marker for the selection of effective stem cells in cell therapy.

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Abstract 249: Cortical-bone Derived Stem Cells Improve Cardiac Outcomes After Myocardial Infarction by Modulating the Inflammatory Response

Circulation Research ◽

10.1161/res.123.suppl_1.249 ◽

2018 ◽

Vol 123 (Suppl_1) ◽

Author(s):

Alexander R Hobby ◽

Thomas E Sharp ◽

Eric Feldsott ◽

Sadia Mohsin ◽

Hajime Kubo ◽

...

Keyword(s):

Myocardial Infarction ◽

Stem Cells ◽

Inflammatory Response ◽

Cortical Bone ◽

Cardiac Outcomes

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Stem Cell Therapy in Cardiovascular Diseases: The Reparative Mechanisms of Mesenchymal Stem Cells for Myocardial Infarction Treatment

Journal of Cell Science & Therapy ◽

10.4172/2157-7013.1000167 ◽

2014 ◽

Vol 05 (03) ◽

Author(s):

Jonghoon Choi

Keyword(s):

Myocardial Infarction ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cardiovascular Diseases ◽

Cell Therapy ◽

Stem Cell Therapy

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Stem Cells as a Resource for Treatment of Infertility-related Diseases

Current Molecular Medicine ◽

10.2174/1566524019666190709172636 ◽

2019 ◽

Vol 19 (8) ◽

pp. 539-546

Author(s):

Jing Wang ◽

Chi Liu ◽

Masayuki Fujino ◽

Guoqing Tong ◽

Qinxiu Zhang ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

In Vitro Fertilization ◽

Cell Therapy ◽

Reproductive Medicine ◽

Intrauterine Insemination ◽

Reproductive Age ◽

Vitro Fertilization ◽

Undifferentiated Cells

Worldwide, infertility affects 8-12% of couples of reproductive age and has become a common problem. There are many ways to treat infertility, including medication, intrauterine insemination, and in vitro fertilization. In recent years, stem-cell therapy has raised new hope in the field of reproductive disability management. Stem cells are self-renewing, self-replicating undifferentiated cells that are capable of producing specialized cells under appropriate conditions. They exist throughout a human’s embryo, fetal, and adult stages and can proliferate into different cells. While many issues remain to be addressed concerning stem cells, stem cells have undeniably opened up new ways to treat infertility. In this review, we describe past, present, and future strategies for the use of stem cells in reproductive medicine.

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Diabetic Retinopathy and Stem Cell Therapy

10.5772/intechopen.100812 ◽

2021 ◽

Author(s):

Sevil Kestane

Keyword(s):

Stem Cells ◽

Diabetic Retinopathy ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Treatment Strategies ◽

Cell Therapies ◽

Long Time

This overview was evaluated by the development of diabetic retinopathy (DR) and the stem cell therapy approach. DR is a microvascular complication of diabetes mellitus, characterized by damage to the retinal blood vessels leading to progressive loss of vision. However, the pathophysiological mechanisms are complicated and not completely understood yet. The current treatment strategies have included medical, laser, intravitreal, and surgical approaches. It is known that the use of mesenchymal stem cells (MSC), which has a great potential, is promising for the treatment of many degenerative disorders, including the eye. In retinal degenerative diseases, MSCs were ameliorated retinal neurons and retinal pigmented epithelial cells in both in vitro and in vivo studies. Stem cell therapies show promise in neurodegenerative diseases. However, it is very important to know which type of stem cell will be used in which situations, the amount of stem cells to be applied, the method of application, and its physiological/neurophysiological effects. Therefore, it is of great importance to evaluate this subject physiologically. After stem cell application, its safety and efficacy should be followed for a long time. In the near future, widespread application of regenerative stem cell therapy may be a standard treatment in DR.

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Fetal liver mesenchymal stem cells restore ovarian function in premature ovarian insufficiency by targeting MT1

Stem Cell Research & Therapy ◽

10.1186/s13287-019-1490-8 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 2

Author(s):

Boxian Huang ◽

Chunfeng Qian ◽

Chenyue Ding ◽

Qingxia Meng ◽

Qinyan Zou ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Ovarian Function ◽

Ovarian Insufficiency ◽

Apoptotic Genes

Abstract Background With the development of regenerative medicine and tissue engineering technology, almost all stem cell therapy is efficacious for the treatment of premature ovarian failure (POF) or premature ovarian insufficiency (POI) animal models, whereas little stem cell therapy has been practiced in clinical settings. The underlying molecular mechanism and safety of stem cell treatment in POI are not fully understood. In this study, we explored whether fetal mesenchymal stem cells (fMSCs) from the liver restore ovarian function and whether melatonin membrane receptor 1 (MT1) acts as a regulator for treating POI disease. Methods We designed an in vivo model (chemotherapy-induced ovary damage) and an in vitro model (human ovarian granulosa cells (hGCs)) to understand the efficacy and molecular cues of fMSC treatment of POI. Follicle development was observed by H&E staining. The concentration of sex hormones in serum (E2, AMH, and FSH) and the concentration of oxidative and antioxidative metabolites and the enzymes MDA, SOD, CAT, LDH, GR, and GPx were measured by ELISA. Flow cytometry (FACS) was employed to detect the percentages of ROS and proliferation rates. mRNA and protein expression of antiapoptotic genes (SURVIVIN and BCL2), apoptotic genes (CASPASE-3 and CASPASE-9), and MT1 and its downstream genes (JNK1, PCNA, AMPK) were tested by qPCR and western blotting. MT1 siRNA and related antagonists were used to assess the mechanism. Results fMSC treatment prevented cyclophosphamide (CTX)-induced follicle loss and recovered sex hormone levels. Additionally, fMSCs significantly decreased oxidative damage, increased oxidative protection, improved antiapoptotic effects, and inhibited apoptotic genes in vivo and in vitro. Furthermore, fMSCs also upregulated MT1, JNK1, PCNA, and AMPK at the mRNA and protein levels. With MT1 knockdown or antagonist treatment in normal hGCs, the protein expression of JNK1, PCNA, and AMPK and the percentage of proliferation were impaired. Conclusions fMSCs might play a crucial role in mediating follicular development in the POI mouse model and stimulating the activity of POI hGCs by targeting MT1.

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Stem cell therapy for acute myocardial infarction - focusing on the comparison between Muse cells and mesenchymal stem cells

Journal of Cardiology ◽

10.1016/j.jjcc.2021.10.030 ◽

2021 ◽

Author(s):

Yoshihisa Yamada ◽

Shingo Minatoguchi ◽

Hiromitsu Kanamori ◽

Atsushi Mikami ◽

Hiroyuki Okura ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Muse Cells

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Effects of nicotine on the translation of stem cell therapy

Regenerative Medicine ◽

10.2217/rme-2020-0032 ◽

2020 ◽

Vol 15 (5) ◽

pp. 1679-1688

Author(s):

Alex HP Chan ◽

Ngan F Huang

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Therapeutic Potential ◽

Electronic Cigarettes ◽

Nicotine Exposure ◽

Cell Therapies ◽

Lifestyle Choices

Although stem cell therapy has tremendous therapeutic potential, clinical translation of stem cell therapy has yet to be fully realized. Recently, patient comorbidities and lifestyle choices have emerged to be important factors in the efficacy of stem cell therapy. Tobacco usage is an important risk factor for numerous diseases, and nicotine exposure specifically has become increasing more prevalent with the rising use of electronic cigarettes. This review describes the effects of nicotine exposure on the function of various stem cells. We place emphasis on the differential effects of nicotine exposure in vitro and as well as in preclinical models. Further research on the effects of nicotine on stem cells will deepen our understanding of how lifestyle choices can impact the outcome of stem cell therapies.

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