Hematocrit value of blood expressed from the isolated perfused kidney

1959 ◽  
Vol 197 (3) ◽  
pp. 571-574 ◽  
Author(s):  
Donald P. Morgan

Isolated dog kidneys were perfused with blood supplied from the carotid artery and returned to the jugular vein. The kidneys were enclosed in a rigid fluid-filled box. When the circulation was temporarily interrupted, about half the estimated total volume of intrarenal blood could be expelled by forcing fluid into the box. Hematocrits of the expressed blood were only slightly lower than hematocrits of arterial and venous blood samples taken during perfusion.

1989 ◽  
Vol 9 (5) ◽  
pp. 717-720 ◽  
Author(s):  
Mogens Jakobsen ◽  
Erna Enevoldsen

Serial measurements of CBF and metabolism require multiple cerebral venous blood samples. Retrograde catheterization of the right internal jugular vein is easily performed at a point 2.5–3 cm lateral to, and 2 cm above, the medial end of the right clavicula. Complications are few and minor. In 1/80 (1.25%) cases the carotid artery was punctured during cannulation. Insertion of the catheter for 16–18 cm reduces contamination with extracerebral blood to a minimum.


1963 ◽  
Vol 26 (2) ◽  
pp. 241-247 ◽  
Author(s):  
I. R. FALCONER

SUMMARY A new method for obtaining thyroid venous blood from an essentially undisturbed ewe has been developed. A lobe of the thyroid gland is transferred to the outside of the neck, in a carotid artery—jugular vein loop. The remaining lobe is removed. The secretory function of the exteriorized lobe has been shown to be normal by 131I studies, measurement of protein bound iodine, response to T.S.H. and histological examination. Cannulation of the jugular vein near the thyroid vein allows continuous or interrupted sampling of thyroid venous blood, the jugular flow being occluded by pressure.


1982 ◽  
Vol 101 (4) ◽  
pp. 517-523 ◽  
Author(s):  
M. Hammer ◽  
H. C. Engell

Abstract. The plasma arginine vasopressin concentration (pAVP) was measured in blood samples obtained from an internal jugular vein of 8 patients who under-went surgery for a carotid artery stenosis. Ten blood samples were taken with 1 min interval during the operation. Arterial pAVP was measured in 10 simultaneous samples from a radial artery and the brain veno-arterial difference of pAVP was calculated. The veno-arterial difference was 0–3 pg/ml in 7 of the patients, while it increased to 35 pg/ml upon baroreceptor stimulation in one patient. A pulsatile pattern was found in the veno-arterial difference of pAVP both at low and higher peripheral pAVP levels. This appeared to reflect a discontinuous release of AVP from the neurohypophysis. The described method results in a more accurate picture of ongoing secretory activity than can be obtained by measurements of peripheral pAVP alone. The general usefulness of the method, however, is restricted by the need of a multitude of samples and the difficult approach to the internal jugular vein.


PEDIATRICS ◽  
1965 ◽  
Vol 36 (5) ◽  
pp. 790-791
Author(s):  
HERBERT S. KAUFMAN

The dangers of venipuncture by an experienced pediatrician are minimal. Nevertheless, some slight risk does attend femoral or jugular vein puncture; moreover, many parents recoil from witnessing femoral blood samples being taken from their healthy child. Many techniques have been described and although laboratory microprocedures may be performed by the "heel-stick" method, many tests require more blood. Anke has modified the technique of Manús and has noted a preference for the scalp veins over other peripheral vessels.Our method for obtaining blood samples requires even less material and does not involve the somewhat hazardous pressure on both jugular veins. PROCEDURE


1975 ◽  
Author(s):  
S. Coccheri ◽  
C. Testa

In a group of patients with Traumatic Brain Laceration (TBL) studies on blood coagulation and platelet function were performed in blood samples obtained during angiography from the omolateral carotid artery and jugular vein. Intraoperatory microbiopsies were also performed during neurosurgical operations in patients with TBL at different stages of evolution. In the acute stages of TBL necrosis of the endothelium and extensive micro-thrombosis with platelet material occurs in the capillaries around the damaged area. In the blood obtained from the jugular vein a fall in platelet count, platelet materials and positive paracoagulation tests can be observed.These data suggest that a process of regional intravascular coagulation occurs after TBL around the damaged area. Resulting microthrombosis may be regarded as a significant factor in the pathogenesis of acute ischemic intracellular edema in neurotraumatology.


2005 ◽  
Vol 68 (4) ◽  
pp. 882-884 ◽  
Author(s):  
R. R. COORE ◽  
S. LOVE ◽  
J. L. McKINSTRY ◽  
H. R. WEAVER ◽  
A. PHILIPS ◽  
...  

Although the incidence of bovine spongiform encephalopathy in cattle continues to decline in the United Kingdom, it remains important to maintain vigilance of all potential routes of transmission of infection to humans. Initial studies have demonstrated a potential risk of carcass contamination with brain tissue following the use of captive bolt gun stunning in cattle. The objective of this study was to further explore these initial findings particularly in regard to captive bolt guns currently in use in the United Kingdom. Brain tissue fragments or elevated levels of a marker protein for brain tissue were detected in venous blood samples from 4% (95% confidence interval, 1.6 to 9.8%) of cattle stunned by penetrating captive bolt gun and from 2% (95% confidence interval, 0.6 to 7%) of those stunned by nonpenetrating captive bolt gun.


1985 ◽  
Vol 249 (6) ◽  
pp. R787-R791 ◽  
Author(s):  
M. A. Baker ◽  
D. D. Dawson

Six dogs were surgically prepared with a right carotid loop and with the left common carotid artery ligated low in the neck. Respiratory frequency (f) and body temperature (Tb) were measured while the animals were at rest in a warm chamber (36–40 degrees C) in four separate experiments: 1) right intracarotid infusion of hypertonic NaCl; 2) right intracarotid infusion of isotonic NaCl; 3) intravenous infusion of hypertonic NaCl; and 4) no infusions. In a fifth experiment, right and left jugular and cephalic venous blood samples were collected before, during, and after the hypertonic intracarotid infusions. A 10-min infusion of hypertonic NaCl into the right common carotid artery elevated right jugular plasma osmolality by 33 mosmol/kg H2O, left jugular plasma osmolality by 26 mosmol/kg H2O, and cephalic plasma osmolality by 8 mosmol/kg H2O. A significant reversible drop in f and a rise in Tb occurred during hypertonic intracarotid infusions. Neither intracarotid infusion of isotonic NaCl nor intravenous infusion of hypertonic NaCl affected f or Tb. In experiments with no infusion, f and Tb did not change. It is concluded that brain receptors sensitive to extracellular solute concentration are able to influence the rate of thermoregulatory panting. This may underlie, in part, the reduced evaporation and elevated body temperatures that occur in dehydrated mammals exposed to heat.


1994 ◽  
Vol 72 (05) ◽  
pp. 672-675 ◽  
Author(s):  
Nicolas W Shammas ◽  
Michael J Cunningham ◽  
Richard M Pomearntz ◽  
Charles W Francis

SummaryTo characterize the extent of early activation of the hemostatic system following angioplasty, we obtained blood samples from the involved coronary artery of 11 stable angina patients during the procedure and measured sensitive markers of thrombin formation (fibrino-peptide A, prothrombin fragment 1.2, and soluble fibrin) and of platelet activation ((3-thromboglobulin). Levels of hemostatic markers in venous blood obtained from 14 young individuals with low pretest probability for coronary artery disease were not significantly different from levels in venous blood or intracoronary samples obtained prior to angioplasty. Also, there was no translesional (proximal and distal to the lesion) gradient in any of the hemostatic markers before or after angioplasty in samples obtained between 18 and 21 min from the onset of the first balloon inflation. Furthermore, no significant difference was noted between angioplasty and postangioplasty intracoronary concentrations. We conclude that intracoronary hemostatic activation does not occur in the majority of patients during and immediately following coronary angioplasty when high doses of heparin and aspirin are administered.


1972 ◽  
Vol 28 (03) ◽  
pp. 383-392 ◽  
Author(s):  
J Hladovec ◽  
Z Koleilat ◽  
I Přerovský

SummaryThe venous occlusion of all four legs in rats caused a highly significant decrease of platelet counts in venous blood especially after the correction for an opposite change in haematocrit. A very pronounced decrease in platelets was observed in human volunteers after a venostasis in one arm in the blood drawn from the occluded limb just before the release of occlusion. Similar decreases were found after a venostasis of both legs in postocclusion blood samples. The decrease in blood platelets results from temporary sequestration in the occluded limbs. The decreases of platelets after a 10 min occlusion of both legs are more pronounced in patients with post thrombotic states.


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